Lactate dehydrogenase-elevating virus (LDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Variarterivirinae; Gammaarterivirus; Gammaarterivirus lacdeh

Average proteome isoelectric point is 8.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q83021|Q83021_LDV ORF 4 OS=Lactate dehydrogenase-elevating virus OX=11048 PE=4 SV=1
MM1 pKa = 7.57AGVNAIFCLFICCSGICFVDD21 pKa = 4.13CNVTASTQVCLWFPIHH37 pKa = 5.86EE38 pKa = 4.36TGKK41 pKa = 7.89THH43 pKa = 7.8LEE45 pKa = 3.67LALNFTLCKK54 pKa = 8.96MCGVVEE60 pKa = 3.97NSYY63 pKa = 10.72YY64 pKa = 10.83SHH66 pKa = 7.53PFCVTDD72 pKa = 3.76DD73 pKa = 3.72TCEE76 pKa = 4.09EE77 pKa = 4.35SSSQYY82 pKa = 11.2LDD84 pKa = 3.72FDD86 pKa = 4.35FPDD89 pKa = 4.38DD90 pKa = 3.9VQLLSSIYY98 pKa = 9.46GTIAINALVFAIHH111 pKa = 5.7QVPSMFGNVSAVFFSHH127 pKa = 7.25NSLCYY132 pKa = 9.1NSTPVEE138 pKa = 4.09HH139 pKa = 6.31NQNVTTSLAIFDD151 pKa = 4.12GDD153 pKa = 3.8VEE155 pKa = 4.01KK156 pKa = 10.15WYY158 pKa = 9.92VLEE161 pKa = 4.23YY162 pKa = 10.31IRR164 pKa = 11.84PLFSSWLVLNVSYY177 pKa = 10.11FLRR180 pKa = 11.84RR181 pKa = 11.84STAGRR186 pKa = 11.84ATRR189 pKa = 11.84RR190 pKa = 11.84LL191 pKa = 3.53

Molecular weight: 21.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83020|Q83020_LDV ORF 3 OS=Lactate dehydrogenase-elevating virus OX=11048 PE=4 SV=1
MM1 pKa = 7.51HH2 pKa = 7.55LFSSLFPSLIFWFSWAFFLGLPSPAGCWFGVSDD35 pKa = 4.29WFSPRR40 pKa = 11.84YY41 pKa = 8.31SVRR44 pKa = 11.84VEE46 pKa = 3.74RR47 pKa = 11.84LTSLTYY53 pKa = 10.1RR54 pKa = 11.84RR55 pKa = 11.84YY56 pKa = 10.0FRR58 pKa = 11.84DD59 pKa = 4.23AIKK62 pKa = 10.51LCQPDD67 pKa = 4.32AIHH70 pKa = 6.04YY71 pKa = 8.14GKK73 pKa = 10.57GFGMLWHH80 pKa = 6.12MKK82 pKa = 9.14IATMLDD88 pKa = 3.72DD89 pKa = 4.89VIHH92 pKa = 6.28SRR94 pKa = 11.84LMSHH98 pKa = 6.75MSHH101 pKa = 6.51QGRR104 pKa = 11.84EE105 pKa = 3.38TWGRR109 pKa = 11.84VFTQKK114 pKa = 10.99ALEE117 pKa = 4.62NIGRR121 pKa = 11.84TQVVSHH127 pKa = 6.54LQQIAGLEE135 pKa = 4.14RR136 pKa = 11.84EE137 pKa = 4.16ACTYY141 pKa = 11.0LMSRR145 pKa = 11.84LPAIQAASAVYY156 pKa = 10.15NVTIKK161 pKa = 10.7FSNEE165 pKa = 3.39TIAISFGPSNTSLTVAEE182 pKa = 4.62HH183 pKa = 6.19LWLVSMQSSVFSSVAAAFALWIVMLLRR210 pKa = 11.84LPRR213 pKa = 11.84SVFGFLSMRR222 pKa = 11.84RR223 pKa = 11.84VRR225 pKa = 11.84RR226 pKa = 11.84TT227 pKa = 2.92

Molecular weight: 26.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1078

115

227

179.7

20.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.865 ± 0.367

3.525 ± 0.689

2.226 ± 0.544

2.876 ± 0.564

6.215 ± 0.568

6.679 ± 0.67

3.711 ± 0.742

5.38 ± 0.429

4.267 ± 0.81

11.132 ± 0.543

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.56

4.731 ± 0.826

3.34 ± 0.138

3.618 ± 0.653

4.36 ± 0.843

8.349 ± 0.979

6.494 ± 0.382

8.72 ± 0.742

1.948 ± 0.371

3.154 ± 0.459
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski