Betacoronavirus HKU24

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; China Rattus coronavirus HKU24

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7UXS1|A0A0A7UXS1_9BETC 2'-O-methyltransferase OS=Betacoronavirus HKU24 OX=1590370 GN=orf1ab PE=3 SV=1
MM1 pKa = 7.49VEE3 pKa = 4.78LSLTDD8 pKa = 3.52TVWYY12 pKa = 9.28VGQIILIVAICLLVILIVVAALATFKK38 pKa = 11.12LCIQLCGMCNTLVVSPSIYY57 pKa = 9.39VYY59 pKa = 10.36NRR61 pKa = 11.84GRR63 pKa = 11.84QFYY66 pKa = 10.6KK67 pKa = 10.25FYY69 pKa = 11.09SDD71 pKa = 3.89AKK73 pKa = 10.65PPVLDD78 pKa = 4.69LDD80 pKa = 4.37DD81 pKa = 3.68VV82 pKa = 3.95

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7UXF2|A0A0A7UXF2_9BETC Nucleoprotein OS=Betacoronavirus HKU24 OX=1590370 GN=N PE=4 SV=1
MM1 pKa = 7.62SDD3 pKa = 3.13SVKK6 pKa = 10.43IIAILVLTPLLIALVVGVCLLHH28 pKa = 7.17LSFVISGLDD37 pKa = 3.26IRR39 pKa = 11.84NTTNPSARR47 pKa = 11.84IGTVCYY53 pKa = 9.77TIRR56 pKa = 11.84TPPTSVVTSGDD67 pKa = 3.55DD68 pKa = 4.02GRR70 pKa = 11.84VTLIKK75 pKa = 9.67PTDD78 pKa = 3.65TYY80 pKa = 10.97TSVYY84 pKa = 9.75LGKK87 pKa = 10.37FRR89 pKa = 11.84GFDD92 pKa = 3.02TGTTGFKK99 pKa = 10.25TPDD102 pKa = 3.33YY103 pKa = 9.55STSTKK108 pKa = 10.4PILTTGKK115 pKa = 9.34PYY117 pKa = 10.94NFRR120 pKa = 11.84HH121 pKa = 5.44LPKK124 pKa = 10.1FRR126 pKa = 11.84RR127 pKa = 11.84LTPTWIPGLL136 pKa = 3.81

Molecular weight: 14.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

10426

82

7141

1042.6

116.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.589 ± 0.514

3.587 ± 0.544

5.64 ± 0.503

3.932 ± 0.364

5.256 ± 0.38

6.206 ± 0.56

1.669 ± 0.237

5.352 ± 0.85

5.86 ± 0.687

8.824 ± 0.601

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.264

5.362 ± 0.809

3.712 ± 0.726

3.635 ± 0.526

3.347 ± 0.505

7.146 ± 0.624

5.851 ± 0.699

9.284 ± 1.164

1.295 ± 0.119

5.189 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski