Amino acid dipepetide frequency for Betacoronavirus HKU24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.659AlaAla: 5.659 ± 0.693
1.918AlaCys: 1.918 ± 0.501
3.741AlaAsp: 3.741 ± 0.999
2.782AlaGlu: 2.782 ± 0.985
3.07AlaPhe: 3.07 ± 0.571
3.549AlaGly: 3.549 ± 0.565
0.863AlaHis: 0.863 ± 0.337
3.645AlaIle: 3.645 ± 0.734
3.645AlaLys: 3.645 ± 0.7
5.755AlaLeu: 5.755 ± 1.358
1.247AlaMet: 1.247 ± 0.523
4.412AlaAsn: 4.412 ± 0.879
2.398AlaPro: 2.398 ± 0.575
2.014AlaGln: 2.014 ± 0.621
2.686AlaArg: 2.686 ± 0.633
4.412AlaSer: 4.412 ± 0.923
3.933AlaThr: 3.933 ± 0.564
5.851AlaVal: 5.851 ± 1.425
0.959AlaTrp: 0.959 ± 0.25
3.357AlaTyr: 3.357 ± 0.46
0.0AlaXaa: 0.0 ± 0.0
Cys
2.686CysAla: 2.686 ± 0.629
1.535CysCys: 1.535 ± 0.368
2.014CysAsp: 2.014 ± 0.637
1.247CysGlu: 1.247 ± 0.408
2.014CysPhe: 2.014 ± 0.179
2.11CysGly: 2.11 ± 0.553
0.384CysHis: 0.384 ± 0.201
1.823CysIle: 1.823 ± 0.369
1.823CysLys: 1.823 ± 0.414
3.07CysLeu: 3.07 ± 0.459
0.48CysMet: 0.48 ± 0.146
2.974CysAsn: 2.974 ± 0.61
1.439CysPro: 1.439 ± 0.461
1.151CysGln: 1.151 ± 0.411
0.959CysArg: 0.959 ± 0.238
2.398CysSer: 2.398 ± 0.664
2.302CysThr: 2.302 ± 0.261
2.878CysVal: 2.878 ± 0.68
0.48CysTrp: 0.48 ± 0.251
2.11CysTyr: 2.11 ± 0.578
0.0CysXaa: 0.0 ± 0.0
Asp
3.933AspAla: 3.933 ± 0.606
2.014AspCys: 2.014 ± 0.502
3.837AspAsp: 3.837 ± 0.633
1.918AspGlu: 1.918 ± 0.327
3.453AspPhe: 3.453 ± 1.086
4.317AspGly: 4.317 ± 0.899
0.863AspHis: 0.863 ± 0.171
3.453AspIle: 3.453 ± 0.409
3.453AspLys: 3.453 ± 0.472
4.317AspLeu: 4.317 ± 0.544
1.823AspMet: 1.823 ± 0.319
3.165AspAsn: 3.165 ± 0.461
1.631AspPro: 1.631 ± 0.589
1.535AspGln: 1.535 ± 0.329
0.959AspArg: 0.959 ± 0.167
3.165AspSer: 3.165 ± 0.484
2.974AspThr: 2.974 ± 0.467
6.043AspVal: 6.043 ± 0.941
0.384AspTrp: 0.384 ± 0.174
3.165AspTyr: 3.165 ± 0.585
0.0AspXaa: 0.0 ± 0.0
Glu
3.453GluAla: 3.453 ± 0.612
1.727GluCys: 1.727 ± 0.448
2.206GluAsp: 2.206 ± 0.653
2.398GluGlu: 2.398 ± 0.363
2.302GluPhe: 2.302 ± 0.53
2.398GluGly: 2.398 ± 0.637
0.576GluHis: 0.576 ± 0.247
2.398GluIle: 2.398 ± 0.646
1.631GluLys: 1.631 ± 0.403
4.125GluLeu: 4.125 ± 0.986
0.767GluMet: 0.767 ± 0.225
0.959GluAsn: 0.959 ± 0.3
1.727GluPro: 1.727 ± 0.902
1.439GluGln: 1.439 ± 0.253
1.439GluArg: 1.439 ± 0.344
2.302GluSer: 2.302 ± 0.427
1.631GluThr: 1.631 ± 0.484
2.878GluVal: 2.878 ± 0.6
0.192GluTrp: 0.192 ± 0.259
2.782GluTyr: 2.782 ± 0.663
0.0GluXaa: 0.0 ± 0.0
Phe
2.686PheAla: 2.686 ± 0.501
2.11PheCys: 2.11 ± 0.48
3.261PheAsp: 3.261 ± 0.641
2.59PheGlu: 2.59 ± 0.732
1.247PhePhe: 1.247 ± 0.378
3.453PheGly: 3.453 ± 1.328
0.671PheHis: 0.671 ± 0.171
3.165PheIle: 3.165 ± 0.918
4.412PheLys: 4.412 ± 0.667
2.782PheLeu: 2.782 ± 0.654
1.151PheMet: 1.151 ± 0.483
3.453PheAsn: 3.453 ± 0.905
1.151PhePro: 1.151 ± 0.308
1.247PheGln: 1.247 ± 0.408
1.439PheArg: 1.439 ± 0.764
3.933PheSer: 3.933 ± 0.492
3.453PheThr: 3.453 ± 0.628
6.235PheVal: 6.235 ± 1.279
0.767PheTrp: 0.767 ± 0.402
3.357PheTyr: 3.357 ± 0.958
0.0PheXaa: 0.0 ± 0.0
Gly
3.357GlyAla: 3.357 ± 0.784
3.165GlyCys: 3.165 ± 0.614
3.07GlyAsp: 3.07 ± 0.527
1.247GlyGlu: 1.247 ± 0.525
3.645GlyPhe: 3.645 ± 0.669
3.549GlyGly: 3.549 ± 0.807
0.863GlyHis: 0.863 ± 0.291
4.221GlyIle: 4.221 ± 1.595
3.261GlyLys: 3.261 ± 1.118
5.084GlyLeu: 5.084 ± 0.869
1.343GlyMet: 1.343 ± 0.389
3.837GlyAsn: 3.837 ± 0.632
1.535GlyPro: 1.535 ± 0.548
1.823GlyGln: 1.823 ± 1.036
2.11GlyArg: 2.11 ± 0.868
5.564GlySer: 5.564 ± 1.381
4.508GlyThr: 4.508 ± 1.067
5.947GlyVal: 5.947 ± 0.926
0.671GlyTrp: 0.671 ± 0.225
3.261GlyTyr: 3.261 ± 0.978
0.0GlyXaa: 0.0 ± 0.0
His
1.631HisAla: 1.631 ± 0.508
0.192HisCys: 0.192 ± 0.157
0.863HisAsp: 0.863 ± 0.221
0.48HisGlu: 0.48 ± 0.18
1.343HisPhe: 1.343 ± 0.347
0.576HisGly: 0.576 ± 0.226
0.192HisHis: 0.192 ± 0.1
1.151HisIle: 1.151 ± 0.526
0.959HisLys: 0.959 ± 0.383
1.535HisLeu: 1.535 ± 0.704
0.48HisMet: 0.48 ± 0.146
0.576HisAsn: 0.576 ± 0.375
0.767HisPro: 0.767 ± 0.255
0.48HisGln: 0.48 ± 0.198
0.288HisArg: 0.288 ± 0.11
0.863HisSer: 0.863 ± 0.262
0.863HisThr: 0.863 ± 0.51
2.398HisVal: 2.398 ± 1.255
0.192HisTrp: 0.192 ± 0.157
0.863HisTyr: 0.863 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
3.261IleAla: 3.261 ± 0.551
1.343IleCys: 1.343 ± 0.537
3.165IleAsp: 3.165 ± 0.364
1.918IleGlu: 1.918 ± 0.377
2.782IlePhe: 2.782 ± 0.419
3.549IleGly: 3.549 ± 0.733
0.384IleHis: 0.384 ± 0.119
3.645IleIle: 3.645 ± 1.697
3.357IleLys: 3.357 ± 0.512
5.564IleLeu: 5.564 ± 1.426
1.151IleMet: 1.151 ± 0.716
3.261IleAsn: 3.261 ± 1.436
2.302IlePro: 2.302 ± 0.52
2.302IleGln: 2.302 ± 0.638
2.494IleArg: 2.494 ± 1.467
3.357IleSer: 3.357 ± 0.715
2.494IleThr: 2.494 ± 0.57
4.7IleVal: 4.7 ± 0.732
0.671IleTrp: 0.671 ± 0.262
1.823IleTyr: 1.823 ± 0.788
0.0IleXaa: 0.0 ± 0.0
Lys
3.741LysAla: 3.741 ± 0.764
2.014LysCys: 2.014 ± 0.417
2.59LysAsp: 2.59 ± 0.446
2.398LysGlu: 2.398 ± 0.517
3.357LysPhe: 3.357 ± 0.693
3.741LysGly: 3.741 ± 0.998
1.535LysHis: 1.535 ± 0.671
2.206LysIle: 2.206 ± 0.291
3.07LysLys: 3.07 ± 0.469
6.715LysLeu: 6.715 ± 1.231
1.247LysMet: 1.247 ± 0.298
2.302LysAsn: 2.302 ± 0.396
3.165LysPro: 3.165 ± 0.967
3.07LysGln: 3.07 ± 1.266
2.494LysArg: 2.494 ± 0.453
3.645LysSer: 3.645 ± 0.457
2.686LysThr: 2.686 ± 0.565
4.604LysVal: 4.604 ± 0.615
1.151LysTrp: 1.151 ± 0.303
2.878LysTyr: 2.878 ± 0.946
0.0LysXaa: 0.0 ± 0.0
Leu
5.755LeuAla: 5.755 ± 1.114
3.261LeuCys: 3.261 ± 1.067
4.796LeuAsp: 4.796 ± 0.538
4.029LeuGlu: 4.029 ± 0.811
4.029LeuPhe: 4.029 ± 1.036
4.221LeuGly: 4.221 ± 0.624
1.727LeuHis: 1.727 ± 0.545
3.741LeuIle: 3.741 ± 0.704
4.892LeuLys: 4.892 ± 0.925
7.962LeuLeu: 7.962 ± 1.708
1.727LeuMet: 1.727 ± 0.518
4.892LeuAsn: 4.892 ± 1.007
3.933LeuPro: 3.933 ± 0.916
3.453LeuGln: 3.453 ± 0.588
2.782LeuArg: 2.782 ± 1.039
7.386LeuSer: 7.386 ± 0.923
6.427LeuThr: 6.427 ± 0.951
7.002LeuVal: 7.002 ± 0.629
1.343LeuTrp: 1.343 ± 0.349
4.892LeuTyr: 4.892 ± 0.984
0.0LeuXaa: 0.0 ± 0.0
Met
1.535MetAla: 1.535 ± 0.247
1.055MetCys: 1.055 ± 0.485
1.151MetAsp: 1.151 ± 0.42
0.48MetGlu: 0.48 ± 0.188
1.918MetPhe: 1.918 ± 0.616
0.959MetGly: 0.959 ± 0.286
0.288MetHis: 0.288 ± 0.151
1.439MetIle: 1.439 ± 0.357
0.48MetLys: 0.48 ± 0.345
2.398MetLeu: 2.398 ± 0.609
0.48MetMet: 0.48 ± 0.146
1.055MetAsn: 1.055 ± 0.169
1.343MetPro: 1.343 ± 0.559
0.863MetGln: 0.863 ± 0.452
0.767MetArg: 0.767 ± 0.314
2.398MetSer: 2.398 ± 0.453
1.151MetThr: 1.151 ± 0.461
1.247MetVal: 1.247 ± 0.397
0.576MetTrp: 0.576 ± 0.505
1.055MetTyr: 1.055 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
2.974AsnAla: 2.974 ± 1.063
2.206AsnCys: 2.206 ± 0.628
2.206AsnAsp: 2.206 ± 0.631
1.535AsnGlu: 1.535 ± 0.153
2.782AsnPhe: 2.782 ± 0.692
4.988AsnGly: 4.988 ± 0.906
0.959AsnHis: 0.959 ± 0.483
2.302AsnIle: 2.302 ± 0.942
3.741AsnLys: 3.741 ± 0.914
4.125AsnLeu: 4.125 ± 0.703
1.247AsnMet: 1.247 ± 0.326
4.029AsnAsn: 4.029 ± 2.139
1.823AsnPro: 1.823 ± 0.528
1.631AsnGln: 1.631 ± 0.418
1.727AsnArg: 1.727 ± 0.585
3.837AsnSer: 3.837 ± 1.315
2.974AsnThr: 2.974 ± 0.961
6.331AsnVal: 6.331 ± 0.863
0.576AsnTrp: 0.576 ± 0.183
1.631AsnTyr: 1.631 ± 0.52
0.0AsnXaa: 0.0 ± 0.0
Pro
2.494ProAla: 2.494 ± 0.282
1.343ProCys: 1.343 ± 0.332
2.398ProAsp: 2.398 ± 0.413
1.535ProGlu: 1.535 ± 0.554
1.727ProPhe: 1.727 ± 0.33
2.686ProGly: 2.686 ± 0.539
0.671ProHis: 0.671 ± 0.303
2.782ProIle: 2.782 ± 0.708
2.302ProLys: 2.302 ± 0.973
3.07ProLeu: 3.07 ± 0.407
0.576ProMet: 0.576 ± 0.226
1.439ProAsn: 1.439 ± 0.905
2.11ProPro: 2.11 ± 0.457
1.343ProGln: 1.343 ± 0.839
0.863ProArg: 0.863 ± 0.487
2.878ProSer: 2.878 ± 1.236
2.878ProThr: 2.878 ± 1.364
2.494ProVal: 2.494 ± 0.669
0.384ProTrp: 0.384 ± 0.351
1.151ProTyr: 1.151 ± 0.498
0.0ProXaa: 0.0 ± 0.0
Gln
2.014GlnAla: 2.014 ± 0.458
0.959GlnCys: 0.959 ± 0.396
1.823GlnAsp: 1.823 ± 0.287
1.535GlnGlu: 1.535 ± 0.288
1.823GlnPhe: 1.823 ± 0.575
2.398GlnGly: 2.398 ± 0.798
0.767GlnHis: 0.767 ± 0.292
1.918GlnIle: 1.918 ± 0.547
2.206GlnLys: 2.206 ± 1.097
3.645GlnLeu: 3.645 ± 0.433
0.671GlnMet: 0.671 ± 0.257
1.918GlnAsn: 1.918 ± 0.818
1.055GlnPro: 1.055 ± 0.604
1.918GlnGln: 1.918 ± 0.531
0.959GlnArg: 0.959 ± 0.533
3.549GlnSer: 3.549 ± 0.891
1.727GlnThr: 1.727 ± 0.521
2.59GlnVal: 2.59 ± 0.304
0.863GlnTrp: 0.863 ± 0.262
2.014GlnTyr: 2.014 ± 0.447
0.0GlnXaa: 0.0 ± 0.0
Arg
2.974ArgAla: 2.974 ± 0.786
0.863ArgCys: 0.863 ± 0.337
2.014ArgAsp: 2.014 ± 0.418
1.343ArgGlu: 1.343 ± 0.372
1.727ArgPhe: 1.727 ± 0.464
1.918ArgGly: 1.918 ± 0.722
0.959ArgHis: 0.959 ± 0.375
1.631ArgIle: 1.631 ± 0.46
1.918ArgLys: 1.918 ± 0.382
3.357ArgLeu: 3.357 ± 0.649
0.48ArgMet: 0.48 ± 0.285
2.206ArgAsn: 2.206 ± 1.13
1.055ArgPro: 1.055 ± 0.305
1.247ArgGln: 1.247 ± 0.492
0.959ArgArg: 0.959 ± 0.589
2.398ArgSer: 2.398 ± 1.212
1.631ArgThr: 1.631 ± 0.804
2.974ArgVal: 2.974 ± 0.781
0.288ArgTrp: 0.288 ± 0.337
1.535ArgTyr: 1.535 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
5.18SerAla: 5.18 ± 0.901
2.59SerCys: 2.59 ± 0.762
4.221SerAsp: 4.221 ± 0.352
3.261SerGlu: 3.261 ± 0.627
3.453SerPhe: 3.453 ± 0.567
4.317SerGly: 4.317 ± 2.729
1.439SerHis: 1.439 ± 0.715
3.645SerIle: 3.645 ± 0.918
3.933SerLys: 3.933 ± 0.742
6.331SerLeu: 6.331 ± 1.396
1.727SerMet: 1.727 ± 0.222
2.11SerAsn: 2.11 ± 0.639
1.727SerPro: 1.727 ± 1.189
3.07SerGln: 3.07 ± 0.469
2.686SerArg: 2.686 ± 2.467
5.659SerSer: 5.659 ± 1.434
4.029SerThr: 4.029 ± 0.667
7.578SerVal: 7.578 ± 1.406
1.151SerTrp: 1.151 ± 0.747
3.357SerTyr: 3.357 ± 0.444
0.0SerXaa: 0.0 ± 0.0
Thr
3.165ThrAla: 3.165 ± 1.019
1.727ThrCys: 1.727 ± 0.55
2.974ThrAsp: 2.974 ± 0.543
1.823ThrGlu: 1.823 ± 0.396
3.549ThrPhe: 3.549 ± 1.039
4.7ThrGly: 4.7 ± 1.225
0.959ThrHis: 0.959 ± 0.319
3.549ThrIle: 3.549 ± 1.311
2.782ThrLys: 2.782 ± 0.368
5.084ThrLeu: 5.084 ± 0.453
2.11ThrMet: 2.11 ± 0.535
2.686ThrAsn: 2.686 ± 0.563
2.206ThrPro: 2.206 ± 1.952
2.206ThrGln: 2.206 ± 0.722
1.727ThrArg: 1.727 ± 0.354
4.221ThrSer: 4.221 ± 1.977
4.125ThrThr: 4.125 ± 0.728
5.468ThrVal: 5.468 ± 0.466
0.48ThrTrp: 0.48 ± 0.237
2.974ThrTyr: 2.974 ± 0.667
0.0ThrXaa: 0.0 ± 0.0
Val
5.564ValAla: 5.564 ± 1.304
3.549ValCys: 3.549 ± 0.845
6.427ValAsp: 6.427 ± 1.552
4.029ValGlu: 4.029 ± 0.59
4.412ValPhe: 4.412 ± 1.181
5.276ValGly: 5.276 ± 1.111
0.959ValHis: 0.959 ± 0.32
4.412ValIle: 4.412 ± 0.745
6.906ValLys: 6.906 ± 2.04
8.153ValLeu: 8.153 ± 1.244
2.686ValMet: 2.686 ± 0.605
4.221ValAsn: 4.221 ± 1.037
3.933ValPro: 3.933 ± 0.633
3.645ValGln: 3.645 ± 0.432
2.878ValArg: 2.878 ± 0.881
5.659ValSer: 5.659 ± 0.855
4.221ValThr: 4.221 ± 1.045
9.113ValVal: 9.113 ± 2.043
0.959ValTrp: 0.959 ± 0.297
5.755ValTyr: 5.755 ± 1.153
0.0ValXaa: 0.0 ± 0.0
Trp
0.576TrpAla: 0.576 ± 0.375
0.384TrpCys: 0.384 ± 0.213
0.48TrpAsp: 0.48 ± 0.251
0.384TrpGlu: 0.384 ± 0.119
1.343TrpPhe: 1.343 ± 0.191
0.288TrpGly: 0.288 ± 0.151
0.48TrpHis: 0.48 ± 0.146
0.671TrpIle: 0.671 ± 0.513
0.288TrpLys: 0.288 ± 0.151
1.631TrpLeu: 1.631 ± 0.468
0.288TrpMet: 0.288 ± 0.217
1.055TrpAsn: 1.055 ± 0.277
0.48TrpPro: 0.48 ± 0.327
0.48TrpGln: 0.48 ± 0.146
0.767TrpArg: 0.767 ± 0.183
0.288TrpSer: 0.288 ± 0.253
0.863TrpThr: 0.863 ± 0.291
1.247TrpVal: 1.247 ± 0.329
0.192TrpTrp: 0.192 ± 0.259
0.767TrpTyr: 0.767 ± 0.523
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.261TyrAla: 3.261 ± 0.474
1.918TyrCys: 1.918 ± 0.289
3.165TyrAsp: 3.165 ± 0.729
2.398TyrGlu: 2.398 ± 0.424
2.59TyrPhe: 2.59 ± 0.415
3.07TyrGly: 3.07 ± 0.584
1.151TyrHis: 1.151 ± 0.32
1.727TyrIle: 1.727 ± 0.308
3.549TyrLys: 3.549 ± 1.066
3.549TyrLeu: 3.549 ± 0.469
0.863TyrMet: 0.863 ± 0.221
3.07TyrAsn: 3.07 ± 0.685
1.343TyrPro: 1.343 ± 0.344
1.439TyrGln: 1.439 ± 0.353
2.494TyrArg: 2.494 ± 0.567
3.549TyrSer: 3.549 ± 0.55
3.645TyrThr: 3.645 ± 0.597
5.276TyrVal: 5.276 ± 0.751
0.671TyrTrp: 0.671 ± 0.162
3.165TyrTyr: 3.165 ± 0.721
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10 proteins (10426 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski