bacterium HR20

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5XX39|A0A2H5XX39_9BACT Uncharacterized protein OS=bacterium HR20 OX=2035415 GN=HRbin20_01359 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 4.68NKK4 pKa = 10.82DD5 pKa = 3.49VLTEE9 pKa = 3.89EE10 pKa = 5.11EE11 pKa = 4.08IAEE14 pKa = 4.72GYY16 pKa = 10.31ILTCQAHH23 pKa = 5.48PTAHH27 pKa = 6.14GVSVDD32 pKa = 3.42YY33 pKa = 11.1DD34 pKa = 3.35QQ35 pKa = 5.85

Molecular weight: 3.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5XUQ2|A0A2H5XUQ2_9BACT Uncharacterized protein OS=bacterium HR20 OX=2035415 GN=HRbin20_00484 PE=4 SV=1
MM1 pKa = 7.39INAASTEE8 pKa = 4.26PFCLEE13 pKa = 3.65ATTGGRR19 pKa = 11.84FLAGILPAAEE29 pKa = 3.98RR30 pKa = 11.84SVVSLPSQQKK40 pKa = 9.21RR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 6.71QTVRR47 pKa = 11.84KK48 pKa = 7.05TRR50 pKa = 11.84KK51 pKa = 4.83TTTVRR56 pKa = 11.84AKK58 pKa = 10.17RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84FAKK64 pKa = 10.09KK65 pKa = 8.16RR66 pKa = 11.84TAPNVSVRR74 pKa = 11.84PGLIRR79 pKa = 11.84GMTIVYY85 pKa = 7.7DD86 pKa = 3.82TLLVPGVIYY95 pKa = 10.54RR96 pKa = 11.84RR97 pKa = 11.84VDD99 pKa = 3.19VEE101 pKa = 4.39LADD104 pKa = 3.68STHH107 pKa = 6.51ALVHH111 pKa = 6.26AVTCVVRR118 pKa = 11.84NPRR121 pKa = 11.84YY122 pKa = 10.12GIEE125 pKa = 4.04LVQAHH130 pKa = 6.8DD131 pKa = 3.3RR132 pKa = 11.84LGRR135 pKa = 11.84LEE137 pKa = 4.25TLPALTRR144 pKa = 11.84RR145 pKa = 11.84LDD147 pKa = 3.47SAQGKK152 pKa = 9.18IVLAAINGSFWRR164 pKa = 11.84AGTRR168 pKa = 11.84QPLGISLSDD177 pKa = 3.68GEE179 pKa = 4.5IVAVTGAPWYY189 pKa = 10.33ALWLDD194 pKa = 3.84RR195 pKa = 11.84RR196 pKa = 11.84WRR198 pKa = 11.84PWLDD202 pKa = 2.97TGGVVATVHH211 pKa = 6.41LSDD214 pKa = 3.49GSTYY218 pKa = 10.53RR219 pKa = 11.84IDD221 pKa = 5.26AINASADD228 pKa = 3.2GVQLFNRR235 pKa = 11.84YY236 pKa = 9.49AGDD239 pKa = 4.31SIPVLPGQIPAEE251 pKa = 4.03LSTAQRR257 pKa = 11.84LITSDD262 pKa = 3.34TLDD265 pKa = 4.03PQWTSDD271 pKa = 3.42SLAAMLSTLQHH282 pKa = 4.44QWQRR286 pKa = 11.84DD287 pKa = 3.64LQKK290 pKa = 10.8PKK292 pKa = 10.9VLLRR296 pKa = 11.84YY297 pKa = 9.17LRR299 pKa = 11.84QPMVNQSTPCLVLRR313 pKa = 11.84TLDD316 pKa = 3.67SEE318 pKa = 4.34RR319 pKa = 11.84VEE321 pKa = 4.37VPLRR325 pKa = 11.84GCVLTVPRR333 pKa = 11.84GHH335 pKa = 7.12PLEE338 pKa = 4.28KK339 pKa = 10.32VIEE342 pKa = 4.27SGKK345 pKa = 10.25LKK347 pKa = 10.82RR348 pKa = 11.84GDD350 pKa = 3.66TVILQVRR357 pKa = 11.84TLRR360 pKa = 11.84NDD362 pKa = 3.96SIPFHH367 pKa = 5.7TAISGTPLLVKK378 pKa = 10.63NGTVKK383 pKa = 10.45PQLEE387 pKa = 4.18DD388 pKa = 3.13TLRR391 pKa = 11.84RR392 pKa = 11.84GSPFVEE398 pKa = 3.9QRR400 pKa = 11.84LSRR403 pKa = 11.84TAIGTDD409 pKa = 3.85VIQSVLYY416 pKa = 10.43LVTVEE421 pKa = 3.94QSPGRR426 pKa = 11.84SVGMSLRR433 pKa = 11.84EE434 pKa = 3.53LAQFMKK440 pKa = 10.64TFGASDD446 pKa = 3.78ALNLDD451 pKa = 4.11GGGSATMVVGDD462 pKa = 4.05RR463 pKa = 11.84CVVPPSGPSQCRR475 pKa = 11.84AVANALVIWRR485 pKa = 11.84RR486 pKa = 11.84KK487 pKa = 8.03PLSRR491 pKa = 5.17

Molecular weight: 53.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1779

0

1779

604333

35

2059

339.7

37.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.458 ± 0.067

1.156 ± 0.025

4.871 ± 0.043

6.053 ± 0.07

3.594 ± 0.035

7.221 ± 0.056

2.181 ± 0.032

5.772 ± 0.041

2.405 ± 0.045

10.469 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.904 ± 0.024

2.65 ± 0.04

5.21 ± 0.037

4.033 ± 0.045

8.0 ± 0.054

6.002 ± 0.052

5.877 ± 0.062

7.671 ± 0.056

1.432 ± 0.025

3.043 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski