Amino acid dipepetide frequency for bacterium HR20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.889AlaAla: 11.889 ± 0.207	1.074AlaCys: 1.074 ± 0.041	4.878AlaAsp: 4.878 ± 0.091	7.003AlaGlu: 7.003 ± 0.14	3.639AlaPhe: 3.639 ± 0.095	6.771AlaGly: 6.771 ± 0.125	2.08AlaHis: 2.08 ± 0.066	6.875AlaIle: 6.875 ± 0.114	2.432AlaLys: 2.432 ± 0.074	12.657AlaLeu: 12.657 ± 0.191	2.295AlaMet: 2.295 ± 0.068	2.325AlaAsn: 2.325 ± 0.064	4.491AlaPro: 4.491 ± 0.098	4.332AlaGln: 4.332 ± 0.087	7.55AlaArg: 7.55 ± 0.12	6.106AlaSer: 6.106 ± 0.119	6.326AlaThr: 6.326 ± 0.124	7.872AlaVal: 7.872 ± 0.121	1.337AlaTrp: 1.337 ± 0.051	2.653AlaTyr: 2.653 ± 0.065	0.0AlaXaa: 0.0 ± 0.0
Cys	1.18CysAla: 1.18 ± 0.045	0.223CysCys: 0.223 ± 0.02	0.708CysAsp: 0.708 ± 0.034	0.467CysGlu: 0.467 ± 0.03	0.374CysPhe: 0.374 ± 0.028	1.097CysGly: 1.097 ± 0.049	0.258CysHis: 0.258 ± 0.021	0.764CysIle: 0.764 ± 0.032	0.276CysLys: 0.276 ± 0.025	0.73CysLeu: 0.73 ± 0.032	0.205CysMet: 0.205 ± 0.02	0.371CysAsn: 0.371 ± 0.021	0.573CysPro: 0.573 ± 0.035	0.437CysGln: 0.437 ± 0.029	0.811CysArg: 0.811 ± 0.042	0.903CysSer: 0.903 ± 0.047	0.854CysThr: 0.854 ± 0.042	0.794CysVal: 0.794 ± 0.038	0.159CysTrp: 0.159 ± 0.017	0.377CysTyr: 0.377 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	5.515AspAla: 5.515 ± 0.103	0.551AspCys: 0.551 ± 0.032	2.598AspAsp: 2.598 ± 0.083	3.043AspGlu: 3.043 ± 0.08	1.723AspPhe: 1.723 ± 0.058	4.411AspGly: 4.411 ± 0.116	0.827AspHis: 0.827 ± 0.04	2.424AspIle: 2.424 ± 0.072	0.92AspLys: 0.92 ± 0.047	3.657AspLeu: 3.657 ± 0.079	0.713AspMet: 0.713 ± 0.034	1.135AspAsn: 1.135 ± 0.051	2.737AspPro: 2.737 ± 0.077	1.483AspGln: 1.483 ± 0.047	3.894AspArg: 3.894 ± 0.087	3.347AspSer: 3.347 ± 0.112	3.017AspThr: 3.017 ± 0.097	4.373AspVal: 4.373 ± 0.081	0.654AspTrp: 0.654 ± 0.034	1.685AspTyr: 1.685 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.787GluAla: 4.787 ± 0.114	0.736GluCys: 0.736 ± 0.04	2.257GluAsp: 2.257 ± 0.064	3.812GluGlu: 3.812 ± 0.124	2.202GluPhe: 2.202 ± 0.062	3.419GluGly: 3.419 ± 0.079	2.26GluHis: 2.26 ± 0.061	3.209GluIle: 3.209 ± 0.088	1.65GluLys: 1.65 ± 0.069	7.511GluLeu: 7.511 ± 0.131	1.041GluMet: 1.041 ± 0.049	1.334GluAsn: 1.334 ± 0.049	2.927GluPro: 2.927 ± 0.074	4.196GluGln: 4.196 ± 0.105	7.637GluArg: 7.637 ± 0.152	2.869GluSer: 2.869 ± 0.077	2.527GluThr: 2.527 ± 0.07	3.427GluVal: 3.427 ± 0.081	0.824GluTrp: 0.824 ± 0.035	1.905GluTyr: 1.905 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	3.836PheAla: 3.836 ± 0.082	0.478PheCys: 0.478 ± 0.028	2.623PheAsp: 2.623 ± 0.067	2.272PheGlu: 2.272 ± 0.063	1.435PhePhe: 1.435 ± 0.055	3.074PheGly: 3.074 ± 0.081	0.758PheHis: 0.758 ± 0.038	1.681PheIle: 1.681 ± 0.062	0.66PheLys: 0.66 ± 0.037	3.503PheLeu: 3.503 ± 0.093	0.521PheMet: 0.521 ± 0.028	0.978PheAsn: 0.978 ± 0.045	1.962PhePro: 1.962 ± 0.057	0.937PheGln: 0.937 ± 0.042	2.732PheArg: 2.732 ± 0.062	2.138PheSer: 2.138 ± 0.063	1.878PheThr: 1.878 ± 0.052	2.846PheVal: 2.846 ± 0.075	0.501PheTrp: 0.501 ± 0.027	1.124PheTyr: 1.124 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	6.75GlyAla: 6.75 ± 0.119	0.956GlyCys: 0.956 ± 0.04	3.685GlyAsp: 3.685 ± 0.09	3.851GlyGlu: 3.851 ± 0.079	2.777GlyPhe: 2.777 ± 0.067	5.81GlyGly: 5.81 ± 0.144	1.304GlyHis: 1.304 ± 0.055	5.49GlyIle: 5.49 ± 0.104	2.821GlyLys: 2.821 ± 0.082	5.447GlyLeu: 5.447 ± 0.104	1.88GlyMet: 1.88 ± 0.062	2.348GlyAsn: 2.348 ± 0.075	1.891GlyPro: 1.891 ± 0.055	2.772GlyGln: 2.772 ± 0.079	4.825GlyArg: 4.825 ± 0.101	4.919GlySer: 4.919 ± 0.113	5.358GlyThr: 5.358 ± 0.123	5.646GlyVal: 5.646 ± 0.108	1.125GlyTrp: 1.125 ± 0.042	2.553GlyTyr: 2.553 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	2.431HisAla: 2.431 ± 0.068	0.356HisCys: 0.356 ± 0.025	0.948HisAsp: 0.948 ± 0.042	1.023HisGlu: 1.023 ± 0.042	0.837HisPhe: 0.837 ± 0.035	1.55HisGly: 1.55 ± 0.053	0.688HisHis: 0.688 ± 0.037	1.129HisIle: 1.129 ± 0.045	0.377HisLys: 0.377 ± 0.026	2.087HisLeu: 2.087 ± 0.066	0.324HisMet: 0.324 ± 0.024	0.515HisAsn: 0.515 ± 0.034	1.579HisPro: 1.579 ± 0.053	0.768HisGln: 0.768 ± 0.038	1.977HisArg: 1.977 ± 0.062	1.218HisSer: 1.218 ± 0.043	1.201HisThr: 1.201 ± 0.043	1.794HisVal: 1.794 ± 0.071	0.291HisTrp: 0.291 ± 0.022	0.716HisTyr: 0.716 ± 0.039	0.0HisXaa: 0.0 ± 0.0
Ile	8.643IleAla: 8.643 ± 0.116	0.536IleCys: 0.536 ± 0.026	2.097IleAsp: 2.097 ± 0.068	5.494IleGlu: 5.494 ± 0.098	1.711IlePhe: 1.711 ± 0.06	5.02IleGly: 5.02 ± 0.091	0.894IleHis: 0.894 ± 0.039	3.02IleIle: 3.02 ± 0.078	1.16IleLys: 1.16 ± 0.053	4.64IleLeu: 4.64 ± 0.09	0.708IleMet: 0.708 ± 0.034	1.415IleAsn: 1.415 ± 0.049	3.26IlePro: 3.26 ± 0.077	1.297IleGln: 1.297 ± 0.045	3.961IleArg: 3.961 ± 0.085	2.833IleSer: 2.833 ± 0.072	3.533IleThr: 3.533 ± 0.083	5.715IleVal: 5.715 ± 0.108	0.483IleTrp: 0.483 ± 0.025	1.297IleTyr: 1.297 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	2.214LysAla: 2.214 ± 0.07	0.199LysCys: 0.199 ± 0.017	0.937LysAsp: 0.937 ± 0.047	1.428LysGlu: 1.428 ± 0.064	0.728LysPhe: 0.728 ± 0.037	1.502LysGly: 1.502 ± 0.05	0.577LysHis: 0.577 ± 0.034	1.368LysIle: 1.368 ± 0.051	0.938LysLys: 0.938 ± 0.048	2.755LysLeu: 2.755 ± 0.077	0.455LysMet: 0.455 ± 0.028	0.738LysAsn: 0.738 ± 0.04	1.459LysPro: 1.459 ± 0.052	1.095LysGln: 1.095 ± 0.046	2.232LysArg: 2.232 ± 0.071	1.248LysSer: 1.248 ± 0.044	1.423LysThr: 1.423 ± 0.049	1.78LysVal: 1.78 ± 0.063	0.25LysTrp: 0.25 ± 0.02	0.72LysTyr: 0.72 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	11.734LeuAla: 11.734 ± 0.167	1.261LeuCys: 1.261 ± 0.049	6.42LeuAsp: 6.42 ± 0.109	7.155LeuGlu: 7.155 ± 0.12	3.559LeuPhe: 3.559 ± 0.091	7.754LeuGly: 7.754 ± 0.134	2.245LeuHis: 2.245 ± 0.067	4.286LeuIle: 4.286 ± 0.092	2.058LeuLys: 2.058 ± 0.069	11.408LeuLeu: 11.408 ± 0.193	1.58LeuMet: 1.58 ± 0.055	2.191LeuAsn: 2.191 ± 0.062	6.098LeuPro: 6.098 ± 0.108	3.899LeuGln: 3.899 ± 0.087	9.609LeuArg: 9.609 ± 0.132	6.149LeuSer: 6.149 ± 0.119	4.703LeuThr: 4.703 ± 0.095	7.982LeuVal: 7.982 ± 0.117	1.489LeuTrp: 1.489 ± 0.063	3.109LeuTyr: 3.109 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	1.663MetAla: 1.663 ± 0.061	0.154MetCys: 0.154 ± 0.016	0.761MetAsp: 0.761 ± 0.036	1.069MetGlu: 1.069 ± 0.043	0.688MetPhe: 0.688 ± 0.035	0.991MetGly: 0.991 ± 0.041	0.485MetHis: 0.485 ± 0.031	0.973MetIle: 0.973 ± 0.043	0.685MetLys: 0.685 ± 0.031	2.682MetLeu: 2.682 ± 0.067	0.448MetMet: 0.448 ± 0.027	0.616MetAsn: 0.616 ± 0.029	1.236MetPro: 1.236 ± 0.04	0.971MetGln: 0.971 ± 0.042	1.557MetArg: 1.557 ± 0.048	1.011MetSer: 1.011 ± 0.047	1.135MetThr: 1.135 ± 0.044	1.322MetVal: 1.322 ± 0.047	0.18MetTrp: 0.18 ± 0.017	0.409MetTyr: 0.409 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.066AsnAla: 3.066 ± 0.072	0.311AsnCys: 0.311 ± 0.024	1.415AsnAsp: 1.415 ± 0.047	1.153AsnGlu: 1.153 ± 0.043	0.981AsnPhe: 0.981 ± 0.044	2.333AsnGly: 2.333 ± 0.084	0.467AsnHis: 0.467 ± 0.03	1.565AsnIle: 1.565 ± 0.055	0.597AsnLys: 0.597 ± 0.031	2.29AsnLeu: 2.29 ± 0.059	0.394AsnMet: 0.394 ± 0.026	0.842AsnAsn: 0.842 ± 0.044	2.087AsnPro: 2.087 ± 0.068	0.862AsnGln: 0.862 ± 0.042	1.878AsnArg: 1.878 ± 0.063	1.289AsnSer: 1.289 ± 0.057	1.519AsnThr: 1.519 ± 0.068	2.33AsnVal: 2.33 ± 0.054	0.314AsnTrp: 0.314 ± 0.024	0.803AsnTyr: 0.803 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	4.699ProAla: 4.699 ± 0.099	0.549ProCys: 0.549 ± 0.031	2.974ProAsp: 2.974 ± 0.068	3.253ProGlu: 3.253 ± 0.084	1.919ProPhe: 1.919 ± 0.051	3.232ProGly: 3.232 ± 0.074	1.249ProHis: 1.249 ± 0.049	3.094ProIle: 3.094 ± 0.077	1.266ProLys: 1.266 ± 0.049	5.439ProLeu: 5.439 ± 0.097	1.153ProMet: 1.153 ± 0.039	1.732ProAsn: 1.732 ± 0.051	3.419ProPro: 3.419 ± 0.087	2.113ProGln: 2.113 ± 0.063	3.117ProArg: 3.117 ± 0.084	3.803ProSer: 3.803 ± 0.093	3.45ProThr: 3.45 ± 0.081	3.432ProVal: 3.432 ± 0.076	0.65ProTrp: 0.65 ± 0.032	1.552ProTyr: 1.552 ± 0.059	0.0ProXaa: 0.0 ± 0.0
Gln	3.49GlnAla: 3.49 ± 0.092	0.47GlnCys: 0.47 ± 0.027	1.215GlnAsp: 1.215 ± 0.044	2.063GlnGlu: 2.063 ± 0.07	1.345GlnPhe: 1.345 ± 0.05	1.982GlnGly: 1.982 ± 0.063	1.124GlnHis: 1.124 ± 0.045	1.981GlnIle: 1.981 ± 0.061	0.887GlnLys: 0.887 ± 0.038	5.427GlnLeu: 5.427 ± 0.114	1.042GlnMet: 1.042 ± 0.039	0.933GlnAsn: 0.933 ± 0.049	2.495GlnPro: 2.495 ± 0.07	2.662GlnGln: 2.662 ± 0.082	4.168GlnArg: 4.168 ± 0.08	2.292GlnSer: 2.292 ± 0.074	2.009GlnThr: 2.009 ± 0.065	2.414GlnVal: 2.414 ± 0.059	1.051GlnTrp: 1.051 ± 0.05	1.277GlnTyr: 1.277 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	7.592ArgAla: 7.592 ± 0.13	1.049ArgCys: 1.049 ± 0.05	3.601ArgAsp: 3.601 ± 0.081	3.894ArgGlu: 3.894 ± 0.093	3.209ArgPhe: 3.209 ± 0.075	5.065ArgGly: 5.065 ± 0.107	1.574ArgHis: 1.574 ± 0.059	5.583ArgIle: 5.583 ± 0.102	2.136ArgLys: 2.136 ± 0.065	7.959ArgLeu: 7.959 ± 0.128	2.082ArgMet: 2.082 ± 0.058	2.292ArgAsn: 2.292 ± 0.062	3.134ArgPro: 3.134 ± 0.069	3.755ArgGln: 3.755 ± 0.076	6.531ArgArg: 6.531 ± 0.141	5.538ArgSer: 5.538 ± 0.113	5.042ArgThr: 5.042 ± 0.097	5.118ArgVal: 5.118 ± 0.109	1.693ArgTrp: 1.693 ± 0.058	3.157ArgTyr: 3.157 ± 0.078	0.0ArgXaa: 0.0 ± 0.0
Ser	6.359SerAla: 6.359 ± 0.108	0.602SerCys: 0.602 ± 0.034	3.412SerAsp: 3.412 ± 0.074	2.848SerGlu: 2.848 ± 0.07	2.113SerPhe: 2.113 ± 0.061	5.197SerGly: 5.197 ± 0.129	1.039SerHis: 1.039 ± 0.046	3.789SerIle: 3.789 ± 0.085	1.329SerLys: 1.329 ± 0.044	6.078SerLeu: 6.078 ± 0.11	1.066SerMet: 1.066 ± 0.04	1.89SerAsn: 1.89 ± 0.068	3.404SerPro: 3.404 ± 0.079	2.106SerGln: 2.106 ± 0.072	3.362SerArg: 3.362 ± 0.086	4.398SerSer: 4.398 ± 0.13	3.812SerThr: 3.812 ± 0.099	4.627SerVal: 4.627 ± 0.088	0.852SerTrp: 0.852 ± 0.042	1.732SerTyr: 1.732 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	6.156ThrAla: 6.156 ± 0.114	0.601ThrCys: 0.601 ± 0.035	2.825ThrAsp: 2.825 ± 0.087	2.8ThrGlu: 2.8 ± 0.075	2.376ThrPhe: 2.376 ± 0.055	4.006ThrGly: 4.006 ± 0.096	1.084ThrHis: 1.084 ± 0.038	4.433ThrIle: 4.433 ± 0.085	1.324ThrLys: 1.324 ± 0.061	6.945ThrLeu: 6.945 ± 0.127	1.056ThrMet: 1.056 ± 0.043	1.665ThrAsn: 1.665 ± 0.077	3.447ThrPro: 3.447 ± 0.081	1.789ThrGln: 1.789 ± 0.053	3.132ThrArg: 3.132 ± 0.074	3.427ThrSer: 3.427 ± 0.113	4.029ThrThr: 4.029 ± 0.108	5.277ThrVal: 5.277 ± 0.116	0.707ThrTrp: 0.707 ± 0.045	1.696ThrTyr: 1.696 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	8.373ValAla: 8.373 ± 0.148	0.798ValCys: 0.798 ± 0.041	2.927ValAsp: 2.927 ± 0.075	5.502ValGlu: 5.502 ± 0.109	2.608ValPhe: 2.608 ± 0.072	5.737ValGly: 5.737 ± 0.101	1.694ValHis: 1.694 ± 0.06	3.917ValIle: 3.917 ± 0.075	1.45ValLys: 1.45 ± 0.058	9.071ValLeu: 9.071 ± 0.147	1.276ValMet: 1.276 ± 0.053	1.746ValAsn: 1.746 ± 0.058	4.215ValPro: 4.215 ± 0.091	2.83ValGln: 2.83 ± 0.074	6.495ValArg: 6.495 ± 0.107	3.816ValSer: 3.816 ± 0.096	4.393ValThr: 4.393 ± 0.103	6.918ValVal: 6.918 ± 0.141	0.938ValTrp: 0.938 ± 0.046	2.01ValTyr: 2.01 ± 0.065	0.0ValXaa: 0.0 ± 0.0
Trp	1.003TrpAla: 1.003 ± 0.043	0.25TrpCys: 0.25 ± 0.021	0.692TrpAsp: 0.692 ± 0.032	0.839TrpGlu: 0.839 ± 0.036	0.503TrpPhe: 0.503 ± 0.026	0.872TrpGly: 0.872 ± 0.039	0.42TrpHis: 0.42 ± 0.027	0.784TrpIle: 0.784 ± 0.037	0.483TrpLys: 0.483 ± 0.027	1.673TrpLeu: 1.673 ± 0.061	0.394TrpMet: 0.394 ± 0.025	0.566TrpAsn: 0.566 ± 0.034	0.212TrpPro: 0.212 ± 0.021	0.842TrpGln: 0.842 ± 0.044	1.33TrpArg: 1.33 ± 0.052	0.981TrpSer: 0.981 ± 0.05	0.773TrpThr: 0.773 ± 0.037	0.836TrpVal: 0.836 ± 0.04	0.354TrpTrp: 0.354 ± 0.026	0.511TrpTyr: 0.511 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.207TyrAla: 3.207 ± 0.079	0.407TyrCys: 0.407 ± 0.028	1.736TyrAsp: 1.736 ± 0.062	1.562TyrGlu: 1.562 ± 0.058	1.21TyrPhe: 1.21 ± 0.039	2.383TyrGly: 2.383 ± 0.066	0.781TyrHis: 0.781 ± 0.041	1.35TyrIle: 1.35 ± 0.046	0.597TyrLys: 0.597 ± 0.037	2.73TyrLeu: 2.73 ± 0.065	0.402TyrMet: 0.402 ± 0.026	0.867TyrAsn: 0.867 ± 0.044	1.473TyrPro: 1.473 ± 0.049	1.183TyrGln: 1.183 ± 0.053	3.204TyrArg: 3.204 ± 0.077	1.731TyrSer: 1.731 ± 0.059	1.79TyrThr: 1.79 ± 0.052	2.209TyrVal: 2.209 ± 0.058	0.465TyrTrp: 0.465 ± 0.031	1.138TyrTyr: 1.138 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1779 proteins (604333 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---