Paracoccus phage vB_PbeS_Pben1
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3T0IJB9|A0A3T0IJB9_9CAUD Type II toxin-antitoxin HicA family toxin OS=Paracoccus phage vB_PbeS_Pben1 OX=2500567 GN=pben1_p26 PE=4 SV=1
MM1 pKa = 6.62 NTAHH5 pKa = 7.47 RR6 pKa = 11.84 LEE8 pKa = 4.85 FFTDD12 pKa = 3.35 ADD14 pKa = 4.19 GEE16 pKa = 4.25 PWACFAWGDD25 pKa = 3.85 VRR27 pKa = 11.84 PEE29 pKa = 3.95 TITRR33 pKa = 11.84 EE34 pKa = 4.2 RR35 pKa = 11.84 ILEE38 pKa = 4.15 AAAYY42 pKa = 8.33 YY43 pKa = 10.92 ADD45 pKa = 3.98 YY46 pKa = 11.51 SEE48 pKa = 6.15 DD49 pKa = 4.06 DD50 pKa = 4.09 LPLEE54 pKa = 4.45 DD55 pKa = 5.56 FEE57 pKa = 4.91 VTRR60 pKa = 11.84 FWIRR64 pKa = 11.84 NSGSSAEE71 pKa = 4.46 FDD73 pKa = 4.24 EE74 pKa = 4.75 MWCRR78 pKa = 11.84 CLAEE82 pKa = 4.91 DD83 pKa = 4.73 DD84 pKa = 4.11 RR85 pKa = 11.84 AVLVTGVQFQQ95 pKa = 3.18
Molecular weight: 11.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.192
IPC2_protein 4.101
IPC_protein 4.024
Toseland 3.834
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.897
Rodwell 3.859
Grimsley 3.745
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.279
Thurlkill 3.872
EMBOSS 3.91
Sillero 4.139
Patrickios 3.401
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.046
Protein with the highest isoelectric point:
>tr|A0A3Q9R813|A0A3Q9R813_9CAUD Uncharacterized protein OS=Paracoccus phage vB_PbeS_Pben1 OX=2500567 GN=pben1_p33 PE=4 SV=1
MM1 pKa = 6.2 VHH3 pKa = 7.02 KK4 pKa = 10.38 SITVLEE10 pKa = 4.19 VAIGILGSKK19 pKa = 9.31 RR20 pKa = 11.84 CWIIRR25 pKa = 11.84 SMSGTRR31 pKa = 11.84 RR32 pKa = 11.84 CTRR35 pKa = 11.84 SGQDD39 pKa = 3.01 GCDD42 pKa = 3.36 GSFGGAGYY50 pKa = 9.24 SVRR53 pKa = 11.84 RR54 pKa = 11.84 SIVSRR59 pKa = 11.84 EE60 pKa = 3.59 FCRR63 pKa = 11.84 PVLLFLKK70 pKa = 9.88 YY71 pKa = 9.86 RR72 pKa = 11.84 AWLAYY77 pKa = 7.27 STRR80 pKa = 11.84 FKK82 pKa = 11.3
Molecular weight: 9.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.78
IPC_protein 10.73
Toseland 10.745
ProMoST 10.628
Dawson 10.847
Bjellqvist 10.628
Wikipedia 11.096
Rodwell 10.965
Grimsley 10.906
Solomon 11.008
Lehninger 10.965
Nozaki 10.76
DTASelect 10.599
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.789
Patrickios 10.716
IPC_peptide 11.008
IPC2_peptide 9.999
IPC2.peptide.svr19 8.424
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
12336
32
731
173.7
19.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.135 ± 0.453
0.948 ± 0.129
6.209 ± 0.216
5.966 ± 0.315
3.283 ± 0.17
8.852 ± 0.276
1.897 ± 0.149
5.05 ± 0.198
3.048 ± 0.27
8.196 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.805 ± 0.13
2.602 ± 0.171
5.869 ± 0.302
3.226 ± 0.134
8.285 ± 0.38
6.153 ± 0.36
4.961 ± 0.264
6.266 ± 0.262
2.213 ± 0.174
2.035 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here