Paracoccus phage vB_PbeS_Pben1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IJB9|A0A3T0IJB9_9CAUD Type II toxin-antitoxin HicA family toxin OS=Paracoccus phage vB_PbeS_Pben1 OX=2500567 GN=pben1_p26 PE=4 SV=1
MM1 pKa = 6.62NTAHH5 pKa = 7.47RR6 pKa = 11.84LEE8 pKa = 4.85FFTDD12 pKa = 3.35ADD14 pKa = 4.19GEE16 pKa = 4.25PWACFAWGDD25 pKa = 3.85VRR27 pKa = 11.84PEE29 pKa = 3.95TITRR33 pKa = 11.84EE34 pKa = 4.2RR35 pKa = 11.84ILEE38 pKa = 4.15AAAYY42 pKa = 8.33YY43 pKa = 10.92ADD45 pKa = 3.98YY46 pKa = 11.51SEE48 pKa = 6.15DD49 pKa = 4.06DD50 pKa = 4.09LPLEE54 pKa = 4.45DD55 pKa = 5.56FEE57 pKa = 4.91VTRR60 pKa = 11.84FWIRR64 pKa = 11.84NSGSSAEE71 pKa = 4.46FDD73 pKa = 4.24EE74 pKa = 4.75MWCRR78 pKa = 11.84CLAEE82 pKa = 4.91DD83 pKa = 4.73DD84 pKa = 4.11RR85 pKa = 11.84AVLVTGVQFQQ95 pKa = 3.18

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R813|A0A3Q9R813_9CAUD Uncharacterized protein OS=Paracoccus phage vB_PbeS_Pben1 OX=2500567 GN=pben1_p33 PE=4 SV=1
MM1 pKa = 6.2VHH3 pKa = 7.02KK4 pKa = 10.38SITVLEE10 pKa = 4.19VAIGILGSKK19 pKa = 9.31RR20 pKa = 11.84CWIIRR25 pKa = 11.84SMSGTRR31 pKa = 11.84RR32 pKa = 11.84CTRR35 pKa = 11.84SGQDD39 pKa = 3.01GCDD42 pKa = 3.36GSFGGAGYY50 pKa = 9.24SVRR53 pKa = 11.84RR54 pKa = 11.84SIVSRR59 pKa = 11.84EE60 pKa = 3.59FCRR63 pKa = 11.84PVLLFLKK70 pKa = 9.88YY71 pKa = 9.86RR72 pKa = 11.84AWLAYY77 pKa = 7.27STRR80 pKa = 11.84FKK82 pKa = 11.3

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12336

32

731

173.7

19.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.135 ± 0.453

0.948 ± 0.129

6.209 ± 0.216

5.966 ± 0.315

3.283 ± 0.17

8.852 ± 0.276

1.897 ± 0.149

5.05 ± 0.198

3.048 ± 0.27

8.196 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.13

2.602 ± 0.171

5.869 ± 0.302

3.226 ± 0.134

8.285 ± 0.38

6.153 ± 0.36

4.961 ± 0.264

6.266 ± 0.262

2.213 ± 0.174

2.035 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski