Apis mellifera associated microvirus 3
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5Y5|A0A3Q8U5Y5_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 3 OX=2494758 PE=4 SV=1
MM1 pKa = 7.32 SKK3 pKa = 10.57 VEE5 pKa = 3.63 IRR7 pKa = 11.84 LPYY10 pKa = 9.06 VQYY13 pKa = 10.97 DD14 pKa = 4.12 DD15 pKa = 5.51 PGLTCPEE22 pKa = 4.08 PTLTQQNFADD32 pKa = 3.66 EE33 pKa = 4.64 CDD35 pKa = 3.89 FNNVLKK41 pKa = 10.58 KK42 pKa = 9.76 WEE44 pKa = 3.89 QSGLITHH51 pKa = 7.24 LNPAQPQYY59 pKa = 11.76 GDD61 pKa = 3.44 VFDD64 pKa = 3.93 IADD67 pKa = 3.66 YY68 pKa = 11.27 QEE70 pKa = 4.44 ALNTVIKK77 pKa = 10.94 AEE79 pKa = 4.02 AAFAALPAALRR90 pKa = 11.84 DD91 pKa = 3.68 RR92 pKa = 11.84 FQNDD96 pKa = 3.46 PANLLAFVEE105 pKa = 5.08 DD106 pKa = 3.55 SANYY110 pKa = 10.03 EE111 pKa = 3.72 EE112 pKa = 4.84 ALRR115 pKa = 11.84 LGLVGPRR122 pKa = 11.84 PSAAEE127 pKa = 3.95 RR128 pKa = 11.84 PHH130 pKa = 6.27 EE131 pKa = 4.69 SGVADD136 pKa = 4.12 EE137 pKa = 4.97 GQPPQAATT145 pKa = 3.34
Molecular weight: 15.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.291
IPC2_protein 4.266
IPC_protein 4.19
Toseland 4.012
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.05
Rodwell 4.024
Grimsley 3.923
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.444
Thurlkill 4.037
EMBOSS 4.062
Sillero 4.304
Patrickios 3.503
IPC_peptide 4.151
IPC2_peptide 4.291
IPC2.peptide.svr19 4.223
Protein with the highest isoelectric point:
>tr|A0A3Q8U5M5|A0A3Q8U5M5_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 3 OX=2494758 PE=3 SV=1
MM1 pKa = 7.37 LNSRR5 pKa = 11.84 SMKK8 pKa = 9.89 RR9 pKa = 11.84 LEE11 pKa = 4.46 GVHH14 pKa = 6.79 PDD16 pKa = 3.24 LVKK19 pKa = 10.83 VVLRR23 pKa = 11.84 AAEE26 pKa = 4.05 LSDD29 pKa = 3.23 VQFIITEE36 pKa = 4.33 GLRR39 pKa = 11.84 SPEE42 pKa = 3.65 RR43 pKa = 11.84 QKK45 pKa = 11.39 RR46 pKa = 11.84 LFEE49 pKa = 4.68 SGASRR54 pKa = 11.84 TLKK57 pKa = 10.34 SRR59 pKa = 11.84 HH60 pKa = 4.73 LTGHH64 pKa = 6.22 AVDD67 pKa = 4.06 VAALVDD73 pKa = 4.47 GAIVWSWHH81 pKa = 5.71 LYY83 pKa = 8.64 PRR85 pKa = 11.84 IAAAFKK91 pKa = 10.35 AAAAEE96 pKa = 4.01 LSVPIVWGGSWPKK109 pKa = 10.25 FRR111 pKa = 11.84 DD112 pKa = 4.12 GPHH115 pKa = 6.49 FEE117 pKa = 4.78 LSSAVYY123 pKa = 10.03 RR124 pKa = 4.26
Molecular weight: 13.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.136
IPC2_protein 9.238
IPC_protein 9.428
Toseland 10.218
ProMoST 10.014
Dawson 10.35
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.716
Grimsley 10.394
Solomon 10.409
Lehninger 10.394
Nozaki 10.175
DTASelect 9.984
Thurlkill 10.233
EMBOSS 10.613
Sillero 10.262
Patrickios 10.496
IPC_peptide 10.423
IPC2_peptide 8.448
IPC2.peptide.svr19 8.407
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1410
86
526
235.0
26.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.064 ± 1.631
0.922 ± 0.522
4.681 ± 0.492
5.461 ± 1.159
5.035 ± 0.655
6.879 ± 0.745
2.482 ± 0.594
3.759 ± 0.331
4.184 ± 0.806
8.582 ± 0.414
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.128 ± 0.389
4.255 ± 1.068
6.028 ± 0.91
5.035 ± 0.542
6.028 ± 0.92
6.241 ± 0.799
5.461 ± 1.049
6.525 ± 0.5
1.418 ± 0.416
3.83 ± 0.835
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here