Aeromicrobium terrae
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3087 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C8NNS0|A0A5C8NNS0_9ACTN Imidazoleglycerol-phosphate dehydratase OS=Aeromicrobium terrae OX=2498846 GN=hisB PE=3 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 IEE4 pKa = 4.13 SRR6 pKa = 11.84 GVLAAVLTVFTLVGAGLLAVASPAGAATANISAEE40 pKa = 4.23 CTPDD44 pKa = 3.44 GNKK47 pKa = 8.78 LTVEE51 pKa = 4.13 LSGYY55 pKa = 10.64 NSGNPSTVVLYY66 pKa = 10.57 INDD69 pKa = 3.85 SVFIAAGFFEE79 pKa = 5.1 GFSGSYY85 pKa = 10.15 SADD88 pKa = 3.2 PTIDD92 pKa = 3.64 VTWKK96 pKa = 10.0 VQVTSSDD103 pKa = 3.98 DD104 pKa = 3.65 PDD106 pKa = 3.78 GTTGATFTKK115 pKa = 9.64 TISVDD120 pKa = 3.11 ACVSSVTPQDD130 pKa = 3.39 PTVTQAQCSGPGEE143 pKa = 4.28 HH144 pKa = 7.1 SDD146 pKa = 3.64 PVVTFATGPDD156 pKa = 3.69 HH157 pKa = 6.6 VTYY160 pKa = 10.36 DD161 pKa = 3.79 YY162 pKa = 11.85 DD163 pKa = 4.24 DD164 pKa = 3.91 ATHH167 pKa = 7.15 LVTATAVATSDD178 pKa = 2.9 MTYY181 pKa = 10.79 RR182 pKa = 11.84 FDD184 pKa = 3.67 TVPAGWTKK192 pKa = 10.72 VDD194 pKa = 3.52 DD195 pKa = 3.61 THH197 pKa = 6.87 ATFEE201 pKa = 4.36 VTLTDD206 pKa = 3.92 PGTCIVEE213 pKa = 4.21 VTPTPPTVVPAQTCGDD229 pKa = 3.73 DD230 pKa = 4.05 VLNVPADD237 pKa = 3.81 TAGITYY243 pKa = 8.69 TRR245 pKa = 11.84 ADD247 pKa = 4.14 DD248 pKa = 4.56 GSSVTATAGTGYY260 pKa = 9.67 EE261 pKa = 4.17 LPGGARR267 pKa = 11.84 SQTWTNRR274 pKa = 11.84 VDD276 pKa = 3.51 YY277 pKa = 10.74 EE278 pKa = 4.27 YY279 pKa = 9.94 DD280 pKa = 3.21 TVANGGAEE288 pKa = 4.18 ACPDD292 pKa = 3.85 PPGDD296 pKa = 4.1 PDD298 pKa = 4.36 DD299 pKa = 5.44 PGVSDD304 pKa = 3.96 SSGPAADD311 pKa = 4.24 ISDD314 pKa = 4.04 SSGVLPDD321 pKa = 4.16 TGSSMTPVVLGIALALLVGGCLVVRR346 pKa = 11.84 RR347 pKa = 11.84 RR348 pKa = 11.84 LTT350 pKa = 3.15
Molecular weight: 35.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.541
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.757
Rodwell 3.592
Grimsley 3.439
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.202
Thurlkill 3.605
EMBOSS 3.757
Sillero 3.91
Patrickios 0.769
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A5C8NG56|A0A5C8NG56_9ACTN 2-oxoacid:ferredoxin oxidoreductase subunit beta OS=Aeromicrobium terrae OX=2498846 GN=FHP06_12475 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.56 KK16 pKa = 9.27 HH17 pKa = 5.36 RR18 pKa = 11.84 KK19 pKa = 5.5 MLKK22 pKa = 7.41 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3087
0
3087
983032
33
3029
318.4
34.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.484 ± 0.066
0.69 ± 0.012
6.839 ± 0.038
5.873 ± 0.046
2.941 ± 0.028
8.928 ± 0.042
2.202 ± 0.022
3.932 ± 0.033
2.443 ± 0.045
9.997 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.949 ± 0.019
1.873 ± 0.026
5.285 ± 0.026
2.777 ± 0.022
7.269 ± 0.054
5.445 ± 0.034
6.134 ± 0.049
9.516 ± 0.045
1.454 ± 0.019
1.968 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here