Microbacterium foliorum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0KRF5|A0A0F0KRF5_9MICO Gamma-aminobutyraldehyde dehydrogenase OS=Microbacterium foliorum OX=104336 GN=prr PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 9.0NTKK5 pKa = 9.99RR6 pKa = 11.84RR7 pKa = 11.84WLTGAVATIGAGALALGGAVVPAAADD33 pKa = 3.75DD34 pKa = 4.43AQDD37 pKa = 4.11GPVEE41 pKa = 4.19LQSSASKK48 pKa = 10.84YY49 pKa = 10.78DD50 pKa = 3.43LAAAQSGTSAEE61 pKa = 4.14EE62 pKa = 3.71IKK64 pKa = 10.9NQEE67 pKa = 3.85EE68 pKa = 3.98AGLISVTDD76 pKa = 3.14KK77 pKa = 11.48GFFYY81 pKa = 10.94FNDD84 pKa = 3.88QIHH87 pKa = 6.61EE88 pKa = 4.35SQSLRR93 pKa = 11.84TLALPAAPEE102 pKa = 4.1GAAIPGTPAAGSRR115 pKa = 11.84PGAPVTVYY123 pKa = 11.16LDD125 pKa = 3.62FDD127 pKa = 4.24GEE129 pKa = 4.6TLEE132 pKa = 4.39GTHH135 pKa = 6.62WNTDD139 pKa = 2.87SGIASLPFAPAAAVTDD155 pKa = 4.8RR156 pKa = 11.84AAVWAAVAEE165 pKa = 4.76DD166 pKa = 3.83YY167 pKa = 11.55APFNVNVTTTRR178 pKa = 11.84PSDD181 pKa = 3.43DD182 pKa = 3.85ALYY185 pKa = 8.52KK186 pKa = 10.38TSQDD190 pKa = 3.41DD191 pKa = 3.64NVYY194 pKa = 9.49GSHH197 pKa = 7.25VIITDD202 pKa = 3.34SYY204 pKa = 12.06DD205 pKa = 3.54EE206 pKa = 4.47VLPAAAGNGGIAWLGGTGSDD226 pKa = 4.12YY227 pKa = 10.92LTGALVFTEE236 pKa = 4.5GTGGSPKK243 pKa = 10.48AIAEE247 pKa = 4.26IAAHH251 pKa = 5.95EE252 pKa = 4.57SGHH255 pKa = 6.01NFGLEE260 pKa = 3.65HH261 pKa = 7.3DD262 pKa = 5.17GISGAGGGEE271 pKa = 4.27YY272 pKa = 10.24YY273 pKa = 10.85APEE276 pKa = 5.26DD277 pKa = 4.25GVWGTIMGAAYY288 pKa = 8.91YY289 pKa = 10.57VPVSQWSAGAYY300 pKa = 10.01AGATNDD306 pKa = 3.54QDD308 pKa = 4.42DD309 pKa = 4.49LATITDD315 pKa = 4.16RR316 pKa = 11.84AAGGPLFVNATLPDD330 pKa = 4.11GSPYY334 pKa = 10.05TGNSVCLPAGSTANPSDD351 pKa = 4.24PKK353 pKa = 10.67PGDD356 pKa = 3.27VIFAVGPNGDD366 pKa = 4.66CNPAGPQLTLNFTWTDD382 pKa = 3.4RR383 pKa = 11.84ADD385 pKa = 3.62YY386 pKa = 10.93AADD389 pKa = 3.66TVGNTPEE396 pKa = 4.05TAQTLDD402 pKa = 3.28NAAGTFGAASVIEE415 pKa = 4.5SNSDD419 pKa = 2.78VDD421 pKa = 4.73VFALTTAGGALTANVQVANISPNLDD446 pKa = 3.08AKK448 pKa = 11.0LSVVDD453 pKa = 3.97ASGAVLAEE461 pKa = 4.27NNPEE465 pKa = 4.0STRR468 pKa = 11.84TSDD471 pKa = 3.03TVAAGLNASVTVPNLEE487 pKa = 4.01AGVYY491 pKa = 8.09YY492 pKa = 10.57LAVEE496 pKa = 4.47GVGAGDD502 pKa = 3.84PATATALNANGYY514 pKa = 9.13DD515 pKa = 3.64AYY517 pKa = 10.77GSLGNYY523 pKa = 6.68TLSGAAEE530 pKa = 4.23PFASAPLVILTPEE543 pKa = 4.84DD544 pKa = 3.59GSAVTGGEE552 pKa = 4.24EE553 pKa = 4.04IDD555 pKa = 3.77VTGTATPGATVTLSVGGAAVDD576 pKa = 3.69TVTADD581 pKa = 4.06GAGAWSGNVTVNQYY595 pKa = 11.14DD596 pKa = 3.29ATEE599 pKa = 3.92IVAAQTVGTIAIPGTTSVTVIAPAAPIAAPAITSPTNGATTDD641 pKa = 3.92DD642 pKa = 4.06STPTISGTGVAGATATVTVRR662 pKa = 11.84NATGGEE668 pKa = 4.12LVGQTTVAADD678 pKa = 4.1GAWSLTLTTALANGEE693 pKa = 4.44YY694 pKa = 10.35VVSAVQASAGEE705 pKa = 4.28TSVSSAAVTFTVAAAPPTGNNNNGGGDD732 pKa = 3.76GNLATTGSDD741 pKa = 2.93FDD743 pKa = 3.79AAPFVGGAALLLLIGAGVLVYY764 pKa = 10.58ARR766 pKa = 11.84RR767 pKa = 11.84RR768 pKa = 11.84SSSVDD773 pKa = 2.84AA774 pKa = 5.09

Molecular weight: 76.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0KMS7|A0A0F0KMS7_9MICO Uncharacterized protein OS=Microbacterium foliorum OX=104336 GN=RN50_01637 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3324

0

3324

1078997

29

2095

324.6

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.397 ± 0.061

0.464 ± 0.009

6.46 ± 0.039

5.609 ± 0.042

3.141 ± 0.023

8.874 ± 0.036

1.938 ± 0.021

4.766 ± 0.033

1.857 ± 0.031

10.059 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.822 ± 0.016

1.898 ± 0.025

5.265 ± 0.03

2.759 ± 0.023

7.232 ± 0.051

5.912 ± 0.03

6.208 ± 0.038

8.884 ± 0.039

1.526 ± 0.017

1.929 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski