Cardiobacterium valvarum
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2618 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A381DW87|A0A381DW87_9GAMM EIIAB-Man OS=Cardiobacterium valvarum OX=194702 GN=manX_1 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.37 YY3 pKa = 10.87 DD4 pKa = 3.87 DD5 pKa = 5.38 ASWHH9 pKa = 5.82 YY10 pKa = 10.97 GGNFPADD17 pKa = 4.05 LPPEE21 pKa = 4.5 AGATHH26 pKa = 6.94 IGMFLAWMLLHH37 pKa = 6.83 GFASNDD43 pKa = 3.5 VQEE46 pKa = 4.73 DD47 pKa = 3.93 AVDD50 pKa = 3.61 EE51 pKa = 4.26 LAALTRR57 pKa = 11.84 RR58 pKa = 11.84 EE59 pKa = 3.62 ISGAEE64 pKa = 3.88 FLCRR68 pKa = 11.84 VCDD71 pKa = 3.52 EE72 pKa = 4.78 ALTDD76 pKa = 4.08 GEE78 pKa = 4.58 FSEE81 pKa = 4.96 EE82 pKa = 4.01 GNAFAAAYY90 pKa = 7.63 YY91 pKa = 10.02 QSEE94 pKa = 4.34 DD95 pKa = 3.59 GEE97 pKa = 5.11 GYY99 pKa = 10.85 GLYY102 pKa = 9.79 IDD104 pKa = 5.92 DD105 pKa = 5.1 YY106 pKa = 10.4 IATFALSDD114 pKa = 3.86 DD115 pKa = 4.22 TDD117 pKa = 4.76 DD118 pKa = 5.43 DD119 pKa = 4.02 NCLYY123 pKa = 10.84 RR124 pKa = 11.84 LDD126 pKa = 5.64 DD127 pKa = 4.71 DD128 pKa = 3.69 WDD130 pKa = 4.16 NYY132 pKa = 11.36 DD133 pKa = 4.17 KK134 pKa = 11.21 LAAVIDD140 pKa = 3.78 KK141 pKa = 10.7 RR142 pKa = 11.84 YY143 pKa = 10.78 GEE145 pKa = 4.33 WLAQGRR151 pKa = 11.84 PASLL155 pKa = 3.33
Molecular weight: 17.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.897
Patrickios 0.629
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A381EBW9|A0A381EBW9_9GAMM NAD(P) transhydrogenase subunit alpha OS=Cardiobacterium valvarum OX=194702 GN=pntA PE=3 SV=1
MM1 pKa = 7.75 KK2 pKa = 10.14 LQISAAHH9 pKa = 6.42 GPGEE13 pKa = 4.42 CEE15 pKa = 3.33 RR16 pKa = 11.84 AAAFALQRR24 pKa = 11.84 LSAEE28 pKa = 4.17 AAAHH32 pKa = 6.53 GIRR35 pKa = 11.84 VEE37 pKa = 3.79 IHH39 pKa = 6.04 DD40 pKa = 4.31 EE41 pKa = 3.74 RR42 pKa = 11.84 RR43 pKa = 11.84 SRR45 pKa = 11.84 HH46 pKa = 5.66 GIQSAVITLSGDD58 pKa = 3.22 EE59 pKa = 4.23 VAAFAARR66 pKa = 11.84 WQGTIQWIWQSRR78 pKa = 11.84 IRR80 pKa = 11.84 PRR82 pKa = 11.84 HH83 pKa = 5.19 PRR85 pKa = 11.84 KK86 pKa = 9.2 NWYY89 pKa = 9.21 IGIYY93 pKa = 9.24 PLAEE97 pKa = 3.94 TAPVARR103 pKa = 11.84 GDD105 pKa = 4.83 ILIQSCKK112 pKa = 10.52 AGGKK116 pKa = 9.81 GGQHH120 pKa = 4.76 VNKK123 pKa = 9.1 TRR125 pKa = 11.84 SAVWATDD132 pKa = 3.41 SVSGLRR138 pKa = 11.84 VKK140 pKa = 10.47 VQAEE144 pKa = 4.33 RR145 pKa = 11.84 SQHH148 pKa = 4.56 ANRR151 pKa = 11.84 RR152 pKa = 11.84 LAYY155 pKa = 8.39 QQLDD159 pKa = 3.44 ARR161 pKa = 11.84 HH162 pKa = 5.82 AALVVEE168 pKa = 4.83 RR169 pKa = 11.84 EE170 pKa = 3.92 AVQRR174 pKa = 11.84 HH175 pKa = 5.08 AQHH178 pKa = 6.4 CRR180 pKa = 11.84 HH181 pKa = 5.56 FQIEE185 pKa = 4.1 RR186 pKa = 11.84 GNPVRR191 pKa = 11.84 IFVGDD196 pKa = 3.8 DD197 pKa = 3.11 FRR199 pKa = 11.84 EE200 pKa = 4.14 RR201 pKa = 11.84 AA202 pKa = 3.45
Molecular weight: 22.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.516
IPC_protein 10.555
Toseland 10.73
ProMoST 10.584
Dawson 10.804
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 10.833
Grimsley 10.847
Solomon 11.008
Lehninger 10.965
Nozaki 10.73
DTASelect 10.584
Thurlkill 10.73
EMBOSS 11.155
Sillero 10.76
Patrickios 10.57
IPC_peptide 11.023
IPC2_peptide 9.838
IPC2.peptide.svr19 8.726
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2618
0
2618
800029
29
4028
305.6
33.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.662 ± 0.099
0.994 ± 0.017
5.87 ± 0.054
5.337 ± 0.048
3.366 ± 0.037
7.385 ± 0.063
2.69 ± 0.032
5.92 ± 0.048
4.142 ± 0.051
10.422 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.104 ± 0.03
3.732 ± 0.05
4.841 ± 0.044
4.131 ± 0.036
6.196 ± 0.051
5.078 ± 0.035
5.668 ± 0.055
5.764 ± 0.057
1.351 ± 0.021
3.345 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here