Labeo rohita (Indian major carp) (Cyprinus rohita)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophy

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498NI06|A0A498NI06_LABRO Butyrophilin subfamily 1 member A1-like isoform X2 OS=Labeo rohita OX=84645 GN=ROHU_004769 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.49VDD4 pKa = 3.69VDD6 pKa = 3.42WSEE9 pKa = 3.98EE10 pKa = 3.94LSVEE14 pKa = 4.14EE15 pKa = 4.47PVNGLLEE22 pKa = 4.14VDD24 pKa = 4.37NDD26 pKa = 3.53WSEE29 pKa = 4.61DD30 pKa = 3.57PTLEE34 pKa = 4.25GPITSRR40 pKa = 11.84SQVDD44 pKa = 4.21DD45 pKa = 3.8DD46 pKa = 3.98WSDD49 pKa = 3.43KK50 pKa = 10.77VFQDD54 pKa = 4.07LVGDD58 pKa = 4.13LSEE61 pKa = 4.09WSTDD65 pKa = 2.99VLYY68 pKa = 11.02ALDD71 pKa = 3.87GVTDD75 pKa = 4.12KK76 pKa = 11.04FHH78 pKa = 7.81SIQTLVAHH86 pKa = 7.08DD87 pKa = 4.42SSDD90 pKa = 3.92DD91 pKa = 3.69VPEE94 pKa = 4.24ATVGTTSDD102 pKa = 3.49SSDD105 pKa = 3.78DD106 pKa = 3.58VPAATVCATSDD117 pKa = 3.49SSNMSDD123 pKa = 3.55SSNDD127 pKa = 3.34VPQSPVCDD135 pKa = 3.59MSDD138 pKa = 3.24SRR140 pKa = 11.84DD141 pKa = 3.86DD142 pKa = 3.6VPEE145 pKa = 3.94ATVCEE150 pKa = 4.24TSSCSDD156 pKa = 3.84DD157 pKa = 3.65VLQATVCPTSDD168 pKa = 3.95CSDD171 pKa = 4.95GIPSAFLCAVSYY183 pKa = 10.97CSDD186 pKa = 3.44GVHH189 pKa = 6.48PSSYY193 pKa = 11.26SEE195 pKa = 3.92MSYY198 pKa = 11.07SSNDD202 pKa = 3.01GFLEE206 pKa = 3.93ATISGTSSYY215 pKa = 11.23SDD217 pKa = 3.86DD218 pKa = 4.1VPPSSCCDD226 pKa = 3.22MSYY229 pKa = 10.89CSSDD233 pKa = 3.8DD234 pKa = 3.52VLEE237 pKa = 4.39ATACQTEE244 pKa = 4.72DD245 pKa = 3.15TVAPQSNQLQNEE257 pKa = 4.53TKK259 pKa = 10.77VIGQFANITHH269 pKa = 6.65ISIHH273 pKa = 5.49QFMPLWNRR281 pKa = 11.84PSAGKK286 pKa = 9.97RR287 pKa = 11.84RR288 pKa = 11.84IWNGLCPTRR297 pKa = 11.84LDD299 pKa = 4.78DD300 pKa = 4.7GFQVALKK307 pKa = 10.14FVSNRR312 pKa = 11.84NKK314 pKa = 10.44KK315 pKa = 9.83FFLIDD320 pKa = 4.36GYY322 pKa = 10.94SKK324 pKa = 10.38PLPLEE329 pKa = 3.86VALLILASQDD339 pKa = 3.35PPVPEE344 pKa = 3.92IVEE347 pKa = 3.99LLDD350 pKa = 3.32WDD352 pKa = 4.4VEE354 pKa = 4.07PDD356 pKa = 3.28HH357 pKa = 6.94YY358 pKa = 11.17ILVLEE363 pKa = 4.51RR364 pKa = 11.84PMSFVEE370 pKa = 4.36LNWFVLRR377 pKa = 11.84QIMSLEE383 pKa = 3.86EE384 pKa = 4.09DD385 pKa = 3.83VAGSSCGRR393 pKa = 11.84QYY395 pKa = 11.32VQLSRR400 pKa = 11.84AADD403 pKa = 3.38VEE405 pKa = 4.63YY406 pKa = 9.44CTGILRR412 pKa = 11.84WKK414 pKa = 9.44TSS416 pKa = 2.83

Molecular weight: 45.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498MHH9|A0A498MHH9_LABRO Tyrosine-protein kinase 223-like OS=Labeo rohita OX=84645 GN=ROHU_035416 PE=4 SV=1
MM1 pKa = 7.59LGFTRR6 pKa = 11.84LQTKK10 pKa = 10.26LGFIRR15 pKa = 11.84LQTKK19 pKa = 10.4LGFTRR24 pKa = 11.84LQTKK28 pKa = 10.42LGFTRR33 pKa = 11.84LQTKK37 pKa = 10.42LGFTRR42 pKa = 11.84LQNMLGFTRR51 pKa = 11.84LQTKK55 pKa = 10.26LGFIRR60 pKa = 11.84LQTKK64 pKa = 10.4LGFTRR69 pKa = 11.84LQTKK73 pKa = 10.42LGFTRR78 pKa = 11.84LQTKK82 pKa = 10.2LGFIRR87 pKa = 11.84LPTRR91 pKa = 11.84LGFTRR96 pKa = 11.84LQTRR100 pKa = 11.84MGFTRR105 pKa = 11.84LQTKK109 pKa = 10.2LGFIRR114 pKa = 11.84LPTRR118 pKa = 11.84LGFTRR123 pKa = 11.84LQTRR127 pKa = 11.84MGFTRR132 pKa = 11.84LQTRR136 pKa = 11.84MGFTRR141 pKa = 11.84LQTRR145 pKa = 11.84LGFIKK150 pKa = 9.69ITDD153 pKa = 3.61QAGLHH158 pKa = 5.83

Molecular weight: 18.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32379

0

32379

16615359

54

13795

513.2

57.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.465 ± 0.016

2.202 ± 0.012

5.334 ± 0.013

6.977 ± 0.025

3.662 ± 0.011

5.978 ± 0.019

2.537 ± 0.006

4.681 ± 0.013

5.843 ± 0.019

9.341 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.007

4.067 ± 0.017

5.412 ± 0.019

4.648 ± 0.018

5.627 ± 0.016

8.481 ± 0.019

5.792 ± 0.018

6.556 ± 0.018

1.177 ± 0.005

2.844 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski