Acholeplasma phage L2 (Bacteriophage L2)

Taxonomy: Viruses; Plasmaviridae; Plasmavirus

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P42549|YO14_BPL2 Uncharacterized 26.1 kDa protein OS=Acholeplasma phage L2 OX=46014 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.52KK3 pKa = 10.46LLTSLMLSTASFMLLLTVSNKK24 pKa = 9.85AYY26 pKa = 10.11AYY28 pKa = 9.99EE29 pKa = 4.04EE30 pKa = 4.64TEE32 pKa = 4.04TFTFEE37 pKa = 4.02IYY39 pKa = 11.29NMNSVVANPGLYY51 pKa = 10.2NYY53 pKa = 10.64LVDD56 pKa = 3.83LTDD59 pKa = 4.25GYY61 pKa = 11.78GYY63 pKa = 11.25GQMQHH68 pKa = 4.74VTVDD72 pKa = 3.52ISLLDD77 pKa = 3.57YY78 pKa = 9.34NTAYY82 pKa = 10.86LEE84 pKa = 4.3KK85 pKa = 10.59NLNGFNLFFTSDD97 pKa = 2.91TGVYY101 pKa = 10.08EE102 pKa = 4.21FGIDD106 pKa = 3.13ASGNIEE112 pKa = 4.47LLNDD116 pKa = 3.9PVAHH120 pKa = 7.15DD121 pKa = 4.51EE122 pKa = 4.22YY123 pKa = 11.5SLTFTGLNNAYY134 pKa = 9.94QDD136 pKa = 3.61LFYY139 pKa = 11.42EE140 pKa = 4.5EE141 pKa = 4.48IVVEE145 pKa = 4.35FEE147 pKa = 3.97IVSFDD152 pKa = 3.42SLEE155 pKa = 4.37GVGTLDD161 pKa = 3.47PNPYY165 pKa = 9.86VVLSEE170 pKa = 4.7LLTEE174 pKa = 4.05NYY176 pKa = 9.54EE177 pKa = 4.22YY178 pKa = 11.37NEE180 pKa = 4.32TIAPFIWVNDD190 pKa = 3.53VQYY193 pKa = 10.95QNYY196 pKa = 9.12YY197 pKa = 9.48SVYY200 pKa = 10.59DD201 pKa = 3.58IGSDD205 pKa = 3.35LALIEE210 pKa = 4.51IDD212 pKa = 4.42GPTLNIYY219 pKa = 9.84SDD221 pKa = 3.36RR222 pKa = 11.84FEE224 pKa = 4.85EE225 pKa = 4.27YY226 pKa = 10.96NEE228 pKa = 4.12TFDD231 pKa = 4.22VGDD234 pKa = 3.6EE235 pKa = 3.71FRR237 pKa = 11.84IIFKK241 pKa = 10.11IYY243 pKa = 8.73KK244 pKa = 8.2TWTQAHH250 pKa = 7.17DD251 pKa = 3.56YY252 pKa = 11.23GRR254 pKa = 11.84IRR256 pKa = 11.84TNASTLYY263 pKa = 10.73LNQQSDD269 pKa = 3.63TSALLSHH276 pKa = 6.53SEE278 pKa = 3.98STNVINVNNIAVGDD292 pKa = 4.36EE293 pKa = 3.97IVMQSPYY300 pKa = 10.4TDD302 pKa = 4.27FSVSYY307 pKa = 10.31GNVGFPRR314 pKa = 11.84TYY316 pKa = 10.6LVEE319 pKa = 4.09QEE321 pKa = 4.45NIAAVKK327 pKa = 9.26MIKK330 pKa = 10.25NSSDD334 pKa = 2.97NTNYY338 pKa = 9.94TFILFYY344 pKa = 10.11WEE346 pKa = 4.03NGSVVSRR353 pKa = 11.84TVNNINLANRR363 pKa = 11.84NSLNIKK369 pKa = 9.92FDD371 pKa = 3.3GGFRR375 pKa = 11.84KK376 pKa = 9.9ALNYY380 pKa = 10.08SDD382 pKa = 5.0VMVANVDD389 pKa = 4.04LLTPLEE395 pKa = 4.54DD396 pKa = 4.17FLPNMVAWDD405 pKa = 5.07AIDD408 pKa = 5.04GDD410 pKa = 3.88ISDD413 pKa = 5.37SIVITDD419 pKa = 3.67NDD421 pKa = 4.62GYY423 pKa = 11.52SPDD426 pKa = 3.38TVGIYY431 pKa = 10.06NVEE434 pKa = 4.0FSVTNSNGQTSSIIAPVHH452 pKa = 5.51VVDD455 pKa = 5.36IVNPVINGVSDD466 pKa = 3.88TVHH469 pKa = 6.48ISYY472 pKa = 10.42DD473 pKa = 3.16QTFNVTNWVNSLTVSDD489 pKa = 4.31NYY491 pKa = 9.98YY492 pKa = 9.31TGLSISIKK500 pKa = 9.89EE501 pKa = 3.93NTYY504 pKa = 9.14TVNKK508 pKa = 9.88NKK510 pKa = 10.51LGTYY514 pKa = 9.64KK515 pKa = 9.4ITVQAVDD522 pKa = 3.43PSGNIGTLTRR532 pKa = 11.84TIVVNDD538 pKa = 4.93GIGPVFNGINTITASINEE556 pKa = 4.7NITVEE561 pKa = 4.1QIKK564 pKa = 10.75AGLAAIDD571 pKa = 5.08AIDD574 pKa = 4.18GNVTTSIVVDD584 pKa = 3.71SDD586 pKa = 3.64NLTGKK591 pKa = 10.51ANTVGVYY598 pKa = 9.58EE599 pKa = 4.36VVFRR603 pKa = 11.84AVDD606 pKa = 3.12AAGNQTFHH614 pKa = 6.44TVTVSIVASPPGFYY628 pKa = 10.06ILNSNSVRR636 pKa = 11.84LLPGANLTIEE646 pKa = 4.25QILNILNASDD656 pKa = 4.44AEE658 pKa = 4.48NISTNYY664 pKa = 8.05TVSVPGTYY672 pKa = 10.35NLSFTLYY679 pKa = 10.42GEE681 pKa = 4.21SHH683 pKa = 5.52QVSITVLGQNDD694 pKa = 4.76SIIPTPVIPGEE705 pKa = 4.28SNPGFNITYY714 pKa = 10.41ALIIGLSAIALLATTVTILNKK735 pKa = 9.61KK736 pKa = 9.61RR737 pKa = 11.84KK738 pKa = 9.02

Molecular weight: 81.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P42541|YO06_BPL2 Uncharacterized 9.8 kDa protein OS=Acholeplasma phage L2 OX=46014 PE=4 SV=1
MM1 pKa = 7.38NIEE4 pKa = 4.18KK5 pKa = 10.76ALMNYY10 pKa = 9.87YY11 pKa = 10.37EE12 pKa = 4.53LTLASYY18 pKa = 10.82EE19 pKa = 4.21DD20 pKa = 3.58STNRR24 pKa = 11.84YY25 pKa = 8.16HH26 pKa = 7.19YY27 pKa = 10.36RR28 pKa = 11.84IVQHH32 pKa = 6.6LIKK35 pKa = 10.07GLKK38 pKa = 8.12YY39 pKa = 10.14IKK41 pKa = 10.09VKK43 pKa = 10.64KK44 pKa = 10.07LEE46 pKa = 4.33KK47 pKa = 10.06VDD49 pKa = 3.13ITIGYY54 pKa = 9.53KK55 pKa = 10.71LIDD58 pKa = 3.48YY59 pKa = 8.36LKK61 pKa = 10.2NHH63 pKa = 5.56THH65 pKa = 6.56NGNNSIKK72 pKa = 10.58KK73 pKa = 9.31IINYY77 pKa = 8.4LRR79 pKa = 11.84KK80 pKa = 9.74VMQHH84 pKa = 5.06YY85 pKa = 10.51RR86 pKa = 11.84ITTSIIDD93 pKa = 4.31LPHH96 pKa = 6.89LPNDD100 pKa = 3.77TKK102 pKa = 10.69PFEE105 pKa = 4.42RR106 pKa = 11.84FYY108 pKa = 11.19HH109 pKa = 7.2DD110 pKa = 4.35DD111 pKa = 3.86LEE113 pKa = 6.8LIMTYY118 pKa = 9.33TKK120 pKa = 10.97NLNSSKK126 pKa = 11.09NSITYY131 pKa = 10.16KK132 pKa = 10.61SFIRR136 pKa = 11.84LLLDD140 pKa = 3.25SGLRR144 pKa = 11.84VSEE147 pKa = 4.06ALNIKK152 pKa = 9.95ISDD155 pKa = 3.48MDD157 pKa = 4.08FKK159 pKa = 11.52NKK161 pKa = 9.59VIRR164 pKa = 11.84VLSSKK169 pKa = 7.56TRR171 pKa = 11.84KK172 pKa = 8.58QRR174 pKa = 11.84YY175 pKa = 8.83APFSSFSLKK184 pKa = 10.45YY185 pKa = 9.1IKK187 pKa = 10.21EE188 pKa = 4.28LIEE191 pKa = 3.98VNPKK195 pKa = 9.85RR196 pKa = 11.84DD197 pKa = 3.4YY198 pKa = 11.49LFYY201 pKa = 11.42NFIKK205 pKa = 10.58DD206 pKa = 3.33RR207 pKa = 11.84QTNKK211 pKa = 10.15NDD213 pKa = 2.59IKK215 pKa = 11.03LFYY218 pKa = 10.41KK219 pKa = 10.46RR220 pKa = 11.84LKK222 pKa = 10.12KK223 pKa = 9.99HH224 pKa = 6.2LNLEE228 pKa = 4.78RR229 pKa = 11.84IHH231 pKa = 5.08THH233 pKa = 5.76RR234 pKa = 11.84FRR236 pKa = 11.84KK237 pKa = 7.82TFASILIEE245 pKa = 4.23NGLNIDD251 pKa = 4.3DD252 pKa = 4.07LQKK255 pKa = 10.67IFDD258 pKa = 3.77HH259 pKa = 6.61SRR261 pKa = 11.84IEE263 pKa = 4.01TTIKK267 pKa = 10.3YY268 pKa = 8.37VQHH271 pKa = 5.74NEE273 pKa = 3.27KK274 pKa = 10.4RR275 pKa = 11.84ALQEE279 pKa = 3.82YY280 pKa = 10.26KK281 pKa = 10.53KK282 pKa = 11.15YY283 pKa = 10.98NDD285 pKa = 3.2WGLNN289 pKa = 3.43

Molecular weight: 34.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

3273

64

738

233.8

26.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.286 ± 0.684

0.183 ± 0.115

5.805 ± 0.321

5.255 ± 0.637

4.919 ± 0.486

5.469 ± 0.626

1.558 ± 0.338

9.96 ± 0.618

7.241 ± 1.313

9.227 ± 0.553

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.955 ± 0.266

7.088 ± 0.819

2.903 ± 0.466

3.33 ± 0.34

3.422 ± 0.594

6.569 ± 0.465

6.386 ± 0.69

6.874 ± 0.827

1.436 ± 0.351

5.133 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski