Bat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Sarbecovirus; Severe acute respiratory syndrome-related coronavirus; Bat SARS CoV Rm1/2004

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0C6F5|R1A_BC279 Replicase polyprotein 1a OS=Bat coronavirus 279/2005 OX=389167 GN=1a PE=3 SV=1
MM1 pKa = 7.71NEE3 pKa = 3.36LTLIDD8 pKa = 5.36FYY10 pKa = 11.69LCFLAFLLFLVLIMLIIFWFSLEE33 pKa = 4.06LQDD36 pKa = 5.95IEE38 pKa = 4.55EE39 pKa = 4.77PCNKK43 pKa = 9.46VV44 pKa = 2.79

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q0Q469|NS8_BC279 Non-structural protein 8 OS=Bat coronavirus 279/2005 OX=389167 GN=8 PE=3 SV=1
MM1 pKa = 7.77SDD3 pKa = 4.56NGPQNQRR10 pKa = 11.84SAPRR14 pKa = 11.84ITFGGPSDD22 pKa = 3.8STDD25 pKa = 3.04NNQDD29 pKa = 3.07GGRR32 pKa = 11.84SGARR36 pKa = 11.84PKK38 pKa = 10.25QRR40 pKa = 11.84RR41 pKa = 11.84PQGLPNNTASWFTALTQHH59 pKa = 6.06GKK61 pKa = 10.36EE62 pKa = 3.96EE63 pKa = 4.07LRR65 pKa = 11.84FPRR68 pKa = 11.84GQGVPINTNSGKK80 pKa = 10.01DD81 pKa = 3.58DD82 pKa = 3.56QIGYY86 pKa = 8.17YY87 pKa = 9.92RR88 pKa = 11.84RR89 pKa = 11.84ATRR92 pKa = 11.84RR93 pKa = 11.84VRR95 pKa = 11.84GGDD98 pKa = 3.05GKK100 pKa = 10.01MKK102 pKa = 10.32KK103 pKa = 10.19LSPRR107 pKa = 11.84WYY109 pKa = 9.82FYY111 pKa = 11.5YY112 pKa = 10.73LGTGPEE118 pKa = 3.63ASLPYY123 pKa = 9.9GANKK127 pKa = 9.97EE128 pKa = 4.24GIVWVATEE136 pKa = 4.37GALNTPKK143 pKa = 10.91DD144 pKa = 4.0HH145 pKa = 7.46IGTRR149 pKa = 11.84NPNNNAAIVLQLPQGTTLPKK169 pKa = 10.34GFYY172 pKa = 10.67AEE174 pKa = 4.5GSRR177 pKa = 11.84GGSQASSRR185 pKa = 11.84SSSRR189 pKa = 11.84SRR191 pKa = 11.84GNSRR195 pKa = 11.84NSTPGSSRR203 pKa = 11.84GNSPARR209 pKa = 11.84MASGSGEE216 pKa = 3.99TALALLLLDD225 pKa = 4.89RR226 pKa = 11.84LNQLEE231 pKa = 4.58SKK233 pKa = 10.86VSGKK237 pKa = 10.04GQQQQGQTVTKK248 pKa = 9.98KK249 pKa = 10.44SAAEE253 pKa = 3.73ASKK256 pKa = 10.76KK257 pKa = 9.61PRR259 pKa = 11.84QKK261 pKa = 9.55RR262 pKa = 11.84TATKK266 pKa = 10.22SYY268 pKa = 11.04NVTQAFGRR276 pKa = 11.84RR277 pKa = 11.84GPEE280 pKa = 3.4QTQGNFGDD288 pKa = 3.74QDD290 pKa = 5.15LIRR293 pKa = 11.84QGTDD297 pKa = 2.9YY298 pKa = 11.01KK299 pKa = 10.7YY300 pKa = 9.82WPQIAQFAPSASAFFGMSRR319 pKa = 11.84IGMEE323 pKa = 4.09VTPLGTWLTYY333 pKa = 10.49HH334 pKa = 7.02GAIKK338 pKa = 10.61LDD340 pKa = 4.18DD341 pKa = 4.89KK342 pKa = 11.64DD343 pKa = 3.91PQFKK347 pKa = 11.14DD348 pKa = 3.7NVILLNKK355 pKa = 10.08HH356 pKa = 4.75IDD358 pKa = 3.32AYY360 pKa = 10.23KK361 pKa = 10.51AFPPTEE367 pKa = 3.95PKK369 pKa = 9.91KK370 pKa = 10.6DD371 pKa = 3.48KK372 pKa = 10.73KK373 pKa = 10.99KK374 pKa = 9.88KK375 pKa = 8.29TDD377 pKa = 3.29EE378 pKa = 4.34AQPLPQRR385 pKa = 11.84KK386 pKa = 8.41KK387 pKa = 10.06QPTVTLLPAADD398 pKa = 3.84MDD400 pKa = 4.26DD401 pKa = 5.3FSRR404 pKa = 11.84QLQNSMSGASADD416 pKa = 3.63STQAA420 pKa = 3.04

Molecular weight: 45.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

14146

44

7079

1178.8

131.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.267 ± 0.245

3.139 ± 0.445

5.182 ± 0.262

5.054 ± 0.415

4.779 ± 0.285

6.016 ± 0.374

1.951 ± 0.242

5.019 ± 0.53

5.691 ± 0.289

9.847 ± 0.518

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.227

4.991 ± 0.245

3.923 ± 0.49

3.662 ± 0.587

3.733 ± 0.501

6.659 ± 0.467

7.034 ± 0.227

8.059 ± 0.699

1.082 ± 0.107

4.51 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski