Blastomyces silverae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8561 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H1BDG1|A0A0H1BDG1_9EURO Uncharacterized protein OS=Blastomyces silverae OX=2060906 GN=EMPG_15100 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.26NGVVGGCGSGSGSGSADD19 pKa = 2.96GGGDD23 pKa = 3.23GGVNGVGSAAGCVGGDD39 pKa = 3.39YY40 pKa = 10.95SLEE43 pKa = 4.19TEE45 pKa = 4.5AEE47 pKa = 4.18HH48 pKa = 7.42PSAHH52 pKa = 7.27DD53 pKa = 3.46EE54 pKa = 4.32ADD56 pKa = 3.16NDD58 pKa = 4.02TPQVKK63 pKa = 10.29DD64 pKa = 3.49RR65 pKa = 11.84MDD67 pKa = 3.26IALHH71 pKa = 5.95NFGDD75 pKa = 4.2YY76 pKa = 10.85SS77 pKa = 3.47

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H1BHU2|A0A0H1BHU2_9EURO Uncharacterized protein OS=Blastomyces silverae OX=2060906 GN=EMPG_13931 PE=4 SV=1
MM1 pKa = 7.01NWIMVAATVPRR12 pKa = 11.84PIRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84VVLRR21 pKa = 11.84VRR23 pKa = 11.84LPVSAGGVGIVRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AWRR40 pKa = 11.84VGMRR44 pKa = 11.84SGIRR48 pKa = 11.84GMGGRR53 pKa = 11.84GGIRR57 pKa = 11.84SGSGRR62 pKa = 11.84GIAIAIGNGEE72 pKa = 4.08RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84SGSGRR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.65RR83 pKa = 3.59

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8561

0

8561

3669030

34

5926

428.6

47.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.041 ± 0.022

1.201 ± 0.009

5.664 ± 0.019

6.251 ± 0.028

3.644 ± 0.018

6.685 ± 0.023

2.448 ± 0.011

5.082 ± 0.018

5.019 ± 0.024

8.863 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.009

3.915 ± 0.016

6.331 ± 0.028

4.037 ± 0.021

6.337 ± 0.022

8.699 ± 0.029

5.919 ± 0.021

5.772 ± 0.017

1.318 ± 0.01

2.659 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski