Parendozoicomonas haliclonae
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4695 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X7AIY5|A0A1X7AIY5_9GAMM Hemin receptor OS=Parendozoicomonas haliclonae OX=1960125 GN=hemR PE=3 SV=1
MM1 pKa = 6.7 FTVGEE6 pKa = 4.27 VTGEE10 pKa = 4.04 VRR12 pKa = 11.84 AVAPDD17 pKa = 3.72 GSSRR21 pKa = 11.84 IVRR24 pKa = 11.84 EE25 pKa = 3.99 GDD27 pKa = 3.33 SLLPGEE33 pKa = 4.3 TLEE36 pKa = 4.91 AEE38 pKa = 4.48 EE39 pKa = 4.56 GAQAILLFPDD49 pKa = 4.19 GRR51 pKa = 11.84 QLALPAGGTLTLPATTLSTQITDD74 pKa = 3.68 LTEE77 pKa = 4.27 SDD79 pKa = 3.81 QSDD82 pKa = 3.77 EE83 pKa = 4.89 LKK85 pKa = 11.03 ASDD88 pKa = 4.95 DD89 pKa = 3.94 SEE91 pKa = 4.67 TIANSQADD99 pKa = 3.6 GMLFPDD105 pKa = 4.02 ISSSGFFEE113 pKa = 4.63 SGPVSYY119 pKa = 10.89 SADD122 pKa = 3.44 SPNHH126 pKa = 5.35 QEE128 pKa = 3.87 NTPAASGQIGGGSSGPIAGLGSTPTLARR156 pKa = 11.84 DD157 pKa = 3.62 GSLGNSLDD165 pKa = 3.46 SGVRR169 pKa = 11.84 DD170 pKa = 3.03 IGYY173 pKa = 7.52 TSSRR177 pKa = 11.84 GPVFSQSSDD186 pKa = 3.17 PGISSGLSTVSSAGAIASSPSTAANSPIALSEE218 pKa = 4.0 ALASGSGASSNTTPIISHH236 pKa = 5.61 PTIVSSSATGGAVISSAQVQTAVSSAPATTSTPSTAPQQTSTQTITPASPSTPEE290 pKa = 3.74 PAAQQATPTPTPEE303 pKa = 4.0 PVVAASTTADD313 pKa = 3.04 TGSVVFGQSLVQQSGLLSNDD333 pKa = 2.72 QGTGITLSRR342 pKa = 11.84 VGDD345 pKa = 3.65 TDD347 pKa = 3.24 ILEE350 pKa = 4.65 TGSTTIQGQYY360 pKa = 10.86 GSLSIQADD368 pKa = 3.06 GMYY371 pKa = 10.1 NYY373 pKa = 8.36 TAASVDD379 pKa = 3.57 LHH381 pKa = 8.53 SDD383 pKa = 3.59 MVAHH387 pKa = 6.95 WAFNEE392 pKa = 4.54 SVGSASVQDD401 pKa = 3.85 SSIMDD406 pKa = 3.82 SVTDD410 pKa = 3.63 TGTLNNNASIVSGGRR425 pKa = 11.84 TGNALQLDD433 pKa = 4.05 GTGDD437 pKa = 3.83 YY438 pKa = 11.39 VSFADD443 pKa = 3.7 SVEE446 pKa = 4.24 INTPTSDD453 pKa = 2.56 ISARR457 pKa = 11.84 TISLFFQPDD466 pKa = 3.43 SATSLSDD473 pKa = 3.04 RR474 pKa = 11.84 QVIYY478 pKa = 10.99 EE479 pKa = 4.33 EE480 pKa = 5.04 GGTTNGFIIYY490 pKa = 9.3 IQSNTLYY497 pKa = 10.26 AGAYY501 pKa = 9.33 SSSTGWTGHH510 pKa = 5.61 YY511 pKa = 10.14 FSTDD515 pKa = 2.42 ISNLDD520 pKa = 3.25 TSQWHH525 pKa = 6.55 SIVLSLDD532 pKa = 2.95 ASTNTMEE539 pKa = 4.13 AWLDD543 pKa = 3.51 GASIGEE549 pKa = 4.04 ISNAQAVASHH559 pKa = 6.71 ASDD562 pKa = 2.95 IRR564 pKa = 11.84 LGGSGDD570 pKa = 3.44 GNLFHH575 pKa = 7.87 DD576 pKa = 5.6 GEE578 pKa = 4.46 QTGAFNFNGKK588 pKa = 8.84 IDD590 pKa = 3.65 EE591 pKa = 4.15 VRR593 pKa = 11.84 VYY595 pKa = 11.05 NRR597 pKa = 11.84 ALNEE601 pKa = 4.03 QEE603 pKa = 4.26 VSLLHH608 pKa = 6.57 SGNTSATEE616 pKa = 3.75 IFNYY620 pKa = 8.83 TATDD624 pKa = 3.55 SAGGTSSSTLTLTVSTPSNISPVAVDD650 pKa = 4.24 DD651 pKa = 4.29 VLSVMSDD658 pKa = 2.59 QSAAINSVAGQGLLGNDD675 pKa = 3.44 RR676 pKa = 11.84 DD677 pKa = 4.19 AEE679 pKa = 4.43 GDD681 pKa = 3.79 SLTVTRR687 pKa = 11.84 VGTTDD692 pKa = 3.01 VAASGTTSITGTYY705 pKa = 7.08 GTLVIAANGTYY716 pKa = 10.06 SYY718 pKa = 11.09 TPLNSAANGGTDD730 pKa = 2.99 TFTYY734 pKa = 9.37 TVSDD738 pKa = 3.6 GTTTSTANLTVNVIADD754 pKa = 4.03 GSANADD760 pKa = 3.57 TLTVTEE766 pKa = 4.72 SGFSASAVYY775 pKa = 9.83 MLDD778 pKa = 3.18 EE779 pKa = 4.93 TGGQDD784 pKa = 3.36 YY785 pKa = 10.61 LAVVDD790 pKa = 4.12 TEE792 pKa = 4.61 SGVSHH797 pKa = 7.29 RR798 pKa = 11.84 LGAITGSSASRR809 pKa = 11.84 KK810 pKa = 6.58 TLAMGPDD817 pKa = 3.21 GTLYY821 pKa = 9.74 VTDD824 pKa = 3.71 STNLYY829 pKa = 8.81 TLDD832 pKa = 4.58 PSTQALTLVGAHH844 pKa = 6.13 GVTDD848 pKa = 3.5 SMSFIGLTVAPDD860 pKa = 3.54 GTIYY864 pKa = 10.06 AAKK867 pKa = 9.85 YY868 pKa = 9.48 DD869 pKa = 3.61 GHH871 pKa = 8.25 IYY873 pKa = 9.34 TLNPSTGAATDD884 pKa = 3.71 LGGVSGFEE892 pKa = 4.23 NNWGDD897 pKa = 4.71 LVWHH901 pKa = 7.25 DD902 pKa = 3.51 GALYY906 pKa = 7.98 TQAYY910 pKa = 7.0 TYY912 pKa = 11.18 TNGQSIYY919 pKa = 9.62 QFVRR923 pKa = 11.84 IEE925 pKa = 3.95 PSNSGFTATPLPNASDD941 pKa = 3.62 AGSLHH946 pKa = 7.2 GITSVNGEE954 pKa = 3.88 LRR956 pKa = 11.84 GVVWSYY962 pKa = 11.2 TGGEE966 pKa = 4.41 TVTIDD971 pKa = 3.34 TTTGLVSSRR980 pKa = 11.84 APVTGAAKK988 pKa = 10.21 IEE990 pKa = 4.44 DD991 pKa = 4.15 SASSGTSMAGNVLTNDD1007 pKa = 3.19 TGTTGVTTVALPDD1020 pKa = 3.94 GTSSTVSNGGATIQGTYY1037 pKa = 8.63 GTLIMQTDD1045 pKa = 3.86 GSYY1048 pKa = 10.23 IYY1050 pKa = 10.38 TLDD1053 pKa = 3.62 NSLEE1057 pKa = 4.1 ATNNLASEE1065 pKa = 4.4 EE1066 pKa = 4.33 TGTDD1070 pKa = 2.63 RR1071 pKa = 11.84 FVYY1074 pKa = 9.17 TASTTGGSTQSILTVFVNGSSEE1096 pKa = 4.57 VVTTDD1101 pKa = 2.64 AATFSNYY1108 pKa = 9.5 NVMTSVDD1115 pKa = 3.86 ASSSTIPIDD1124 pKa = 4.05 FTITTQNDD1132 pKa = 3.04 SDD1134 pKa = 4.81 KK1135 pKa = 9.86 ITEE1138 pKa = 4.06 IVISGVNGSTFNVPSVLTDD1157 pKa = 2.94 NGTITGNTSNSSEE1170 pKa = 4.35 FVWRR1174 pKa = 11.84 ATPGTNAGLDD1184 pKa = 3.67 SFDD1187 pKa = 3.86 AASAGLTLATINGSQAWGQHH1207 pKa = 4.76 MGISATVSEE1216 pKa = 4.59 YY1217 pKa = 11.22 DD1218 pKa = 3.59 SNGATTSSWTSTSAIHH1234 pKa = 5.41 TTLAGIDD1241 pKa = 3.5 HH1242 pKa = 6.81 TRR1244 pKa = 11.84 TGTSGDD1250 pKa = 3.63 DD1251 pKa = 3.52 TLTGSSDD1258 pKa = 2.45 TWTNIDD1264 pKa = 4.08 EE1265 pKa = 5.23 FIGGAGNDD1273 pKa = 3.58 TMTGGSGSDD1282 pKa = 3.15 FYY1284 pKa = 11.55 VWRR1287 pKa = 11.84 GADD1290 pKa = 3.31 VGTSSSPAIDD1300 pKa = 4.2 TITDD1304 pKa = 4.0 FSAGRR1309 pKa = 11.84 LGDD1312 pKa = 3.74 VLDD1315 pKa = 4.56 LRR1317 pKa = 11.84 EE1318 pKa = 4.8 LLPDD1322 pKa = 3.67 TAEE1325 pKa = 4.05 STLDD1329 pKa = 3.37 EE1330 pKa = 4.4 FLSFSFADD1338 pKa = 3.79 GNTTIGVSTTAGGPVVQNIVLDD1360 pKa = 4.03 NVDD1363 pKa = 4.19 LSSSFGTTDD1372 pKa = 2.93 VTQLTNQLTDD1382 pKa = 3.06 NGNLLTT1388 pKa = 4.76
Molecular weight: 141.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.694
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 1.367
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A1X7AJD2|A0A1X7AJD2_9GAMM Histone H1-like protein HC2 OS=Parendozoicomonas haliclonae OX=1960125 GN=hctB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 AVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.7 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4695
0
4695
1626144
29
7743
346.4
38.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.909 ± 0.035
1.106 ± 0.014
5.6 ± 0.04
6.361 ± 0.037
3.781 ± 0.025
7.297 ± 0.034
2.258 ± 0.018
5.612 ± 0.029
4.605 ± 0.038
10.484 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.702 ± 0.022
3.838 ± 0.028
4.551 ± 0.031
4.556 ± 0.033
5.338 ± 0.03
6.68 ± 0.037
5.511 ± 0.033
6.707 ± 0.031
1.295 ± 0.013
2.807 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here