Oscillibacter sp. PC13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Oscillibacter; unclassified Oscillibacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5SCQ1|A0A1I5SCQ1_9FIRM Ferrous iron transport protein B OS=Oscillibacter sp. PC13 OX=1855299 GN=SAMN05216343_11216 PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.51KK3 pKa = 10.26FFALLLTLACVLSLAACGSEE23 pKa = 3.97AAPTEE28 pKa = 4.38TPDD31 pKa = 3.22NQTNEE36 pKa = 4.44DD37 pKa = 3.89PADD40 pKa = 3.58TAFTTVEE47 pKa = 3.85EE48 pKa = 5.1GKK50 pKa = 10.33LHH52 pKa = 6.13MATNAAFPPYY62 pKa = 10.98EE63 pKa = 4.16MTTDD67 pKa = 3.08AGGFEE72 pKa = 5.05GIDD75 pKa = 3.27VDD77 pKa = 4.16VAAAIAEE84 pKa = 4.24KK85 pKa = 10.65LGLEE89 pKa = 4.17LVVDD93 pKa = 4.49DD94 pKa = 4.76MDD96 pKa = 4.27FNSVITAVQQGSSDD110 pKa = 2.83IAMAGLTVNEE120 pKa = 3.94EE121 pKa = 3.93RR122 pKa = 11.84KK123 pKa = 10.16QNVDD127 pKa = 3.76FTASYY132 pKa = 8.43ATGVQVVIVKK142 pKa = 10.34AGSDD146 pKa = 3.07ITLDD150 pKa = 3.59NLGDD154 pKa = 3.77QMIGTQEE161 pKa = 3.92STTGYY166 pKa = 9.63IYY168 pKa = 10.94CADD171 pKa = 4.05EE172 pKa = 4.35FGEE175 pKa = 4.3DD176 pKa = 3.69HH177 pKa = 7.12VIAYY181 pKa = 7.51TNGATAVQSLLAGSVDD197 pKa = 3.77CVVIDD202 pKa = 4.2NEE204 pKa = 4.05PAKK207 pKa = 10.88AFVAANPDD215 pKa = 3.55LTILDD220 pKa = 3.54TEE222 pKa = 4.67YY223 pKa = 11.17AVEE226 pKa = 4.88DD227 pKa = 3.86YY228 pKa = 11.16AIGVSKK234 pKa = 10.58EE235 pKa = 3.73NSALLEE241 pKa = 4.34AVDD244 pKa = 4.4TALQDD249 pKa = 4.53LIADD253 pKa = 4.12GTVQSIVDD261 pKa = 3.46KK262 pKa = 11.13YY263 pKa = 9.58ITAEE267 pKa = 3.75

Molecular weight: 28.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5U3E9|A0A1I5U3E9_9FIRM 30S ribosomal protein S20 OS=Oscillibacter sp. PC13 OX=1855299 GN=rpsT PE=3 SV=1
MM1 pKa = 7.51AFDD4 pKa = 3.87RR5 pKa = 11.84EE6 pKa = 4.29ARR8 pKa = 11.84PARR11 pKa = 11.84PMRR14 pKa = 11.84GKK16 pKa = 10.06RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.1VCQFCADD26 pKa = 3.26KK27 pKa = 11.23CEE29 pKa = 4.7SIDD32 pKa = 3.69YY33 pKa = 10.71KK34 pKa = 11.09DD35 pKa = 3.24AAKK38 pKa = 10.4LRR40 pKa = 11.84RR41 pKa = 11.84FVSEE45 pKa = 3.77RR46 pKa = 11.84SKK48 pKa = 10.62ILPRR52 pKa = 11.84RR53 pKa = 11.84TTGTCAMHH61 pKa = 6.16QRR63 pKa = 11.84QLTEE67 pKa = 4.31AIKK70 pKa = 10.35RR71 pKa = 11.84ARR73 pKa = 11.84QIALLPYY80 pKa = 9.27VTDD83 pKa = 3.53

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2711

0

2711

811945

25

4336

299.5

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.837 ± 0.058

1.732 ± 0.025

5.423 ± 0.049

6.702 ± 0.05

3.829 ± 0.032

7.915 ± 0.046

1.869 ± 0.024

5.803 ± 0.045

4.799 ± 0.049

9.959 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.939 ± 0.03

3.333 ± 0.03

4.098 ± 0.028

3.416 ± 0.026

5.408 ± 0.054

5.566 ± 0.043

5.68 ± 0.069

7.201 ± 0.04

1.044 ± 0.019

3.447 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski