Amino acid dipepetide frequency for Oscillibacter sp. PC13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.01AlaAla: 13.01 ± 0.169	1.713AlaCys: 1.713 ± 0.051	5.782AlaAsp: 5.782 ± 0.096	7.207AlaGlu: 7.207 ± 0.115	3.595AlaPhe: 3.595 ± 0.076	8.007AlaGly: 8.007 ± 0.106	1.445AlaHis: 1.445 ± 0.041	5.33AlaIle: 5.33 ± 0.104	4.575AlaLys: 4.575 ± 0.085	9.921AlaLeu: 9.921 ± 0.139	3.067AlaMet: 3.067 ± 0.066	2.581AlaAsn: 2.581 ± 0.063	3.303AlaPro: 3.303 ± 0.07	3.208AlaGln: 3.208 ± 0.06	4.334AlaArg: 4.334 ± 0.081	4.697AlaSer: 4.697 ± 0.075	3.912AlaThr: 3.912 ± 0.086	8.714AlaVal: 8.714 ± 0.126	0.892AlaTrp: 0.892 ± 0.037	3.075AlaTyr: 3.075 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	1.626CysAla: 1.626 ± 0.048	0.405CysCys: 0.405 ± 0.028	0.974CysAsp: 0.974 ± 0.038	0.883CysGlu: 0.883 ± 0.04	0.69CysPhe: 0.69 ± 0.029	1.983CysGly: 1.983 ± 0.059	0.366CysHis: 0.366 ± 0.023	1.028CysIle: 1.028 ± 0.033	0.67CysLys: 0.67 ± 0.029	1.477CysLeu: 1.477 ± 0.048	0.456CysMet: 0.456 ± 0.024	0.502CysAsn: 0.502 ± 0.028	0.831CysPro: 0.831 ± 0.036	0.486CysGln: 0.486 ± 0.023	1.107CysArg: 1.107 ± 0.036	0.983CysSer: 0.983 ± 0.031	0.964CysThr: 0.964 ± 0.033	1.243CysVal: 1.243 ± 0.044	0.139CysTrp: 0.139 ± 0.011	0.506CysTyr: 0.506 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	5.329AspAla: 5.329 ± 0.086	0.893AspCys: 0.893 ± 0.034	2.552AspAsp: 2.552 ± 0.067	3.548AspGlu: 3.548 ± 0.073	2.366AspPhe: 2.366 ± 0.052	5.089AspGly: 5.089 ± 0.1	0.985AspHis: 0.985 ± 0.039	3.338AspIle: 3.338 ± 0.066	2.226AspLys: 2.226 ± 0.061	5.314AspLeu: 5.314 ± 0.09	1.621AspMet: 1.621 ± 0.047	1.514AspAsn: 1.514 ± 0.039	2.437AspPro: 2.437 ± 0.06	1.551AspGln: 1.551 ± 0.046	2.908AspArg: 2.908 ± 0.057	2.679AspSer: 2.679 ± 0.072	3.208AspThr: 3.208 ± 0.067	3.803AspVal: 3.803 ± 0.077	0.693AspTrp: 0.693 ± 0.026	2.176AspTyr: 2.176 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	6.563GluAla: 6.563 ± 0.106	0.687GluCys: 0.687 ± 0.028	3.58GluAsp: 3.58 ± 0.066	5.601GluGlu: 5.601 ± 0.102	2.065GluPhe: 2.065 ± 0.043	4.179GluGly: 4.179 ± 0.082	1.397GluHis: 1.397 ± 0.046	4.189GluIle: 4.189 ± 0.072	4.354GluLys: 4.354 ± 0.085	6.553GluLeu: 6.553 ± 0.097	2.083GluMet: 2.083 ± 0.05	3.106GluAsn: 3.106 ± 0.07	2.065GluPro: 2.065 ± 0.06	2.928GluGln: 2.928 ± 0.079	3.989GluArg: 3.989 ± 0.082	3.154GluSer: 3.154 ± 0.065	3.946GluThr: 3.946 ± 0.091	3.924GluVal: 3.924 ± 0.086	0.583GluTrp: 0.583 ± 0.026	2.07GluTyr: 2.07 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.615PheAla: 3.615 ± 0.073	0.836PheCys: 0.836 ± 0.031	2.346PheAsp: 2.346 ± 0.057	1.984PheGlu: 1.984 ± 0.057	1.709PhePhe: 1.709 ± 0.059	3.239PheGly: 3.239 ± 0.068	0.855PheHis: 0.855 ± 0.033	1.937PheIle: 1.937 ± 0.059	1.262PheLys: 1.262 ± 0.037	4.115PheLeu: 4.115 ± 0.09	0.887PheMet: 0.887 ± 0.036	1.197PheAsn: 1.197 ± 0.041	1.654PhePro: 1.654 ± 0.043	1.301PheGln: 1.301 ± 0.036	1.988PheArg: 1.988 ± 0.056	2.777PheSer: 2.777 ± 0.054	2.285PheThr: 2.285 ± 0.055	2.452PheVal: 2.452 ± 0.066	0.495PheTrp: 0.495 ± 0.023	1.36PheTyr: 1.36 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	6.848GlyAla: 6.848 ± 0.104	1.599GlyCys: 1.599 ± 0.045	3.972GlyAsp: 3.972 ± 0.078	4.806GlyGlu: 4.806 ± 0.078	3.126GlyPhe: 3.126 ± 0.062	6.688GlyGly: 6.688 ± 0.114	1.532GlyHis: 1.532 ± 0.047	5.426GlyIle: 5.426 ± 0.095	4.596GlyLys: 4.596 ± 0.077	7.179GlyLeu: 7.179 ± 0.132	2.6GlyMet: 2.6 ± 0.069	2.599GlyAsn: 2.599 ± 0.058	1.924GlyPro: 1.924 ± 0.053	2.464GlyGln: 2.464 ± 0.056	4.109GlyArg: 4.109 ± 0.072	4.486GlySer: 4.486 ± 0.086	5.146GlyThr: 5.146 ± 0.099	6.063GlyVal: 6.063 ± 0.096	0.906GlyTrp: 0.906 ± 0.038	3.085GlyTyr: 3.085 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	1.495HisAla: 1.495 ± 0.045	0.4HisCys: 0.4 ± 0.025	0.973HisAsp: 0.973 ± 0.034	0.991HisGlu: 0.991 ± 0.044	0.798HisPhe: 0.798 ± 0.032	1.505HisGly: 1.505 ± 0.039	0.484HisHis: 0.484 ± 0.028	1.256HisIle: 1.256 ± 0.041	0.702HisLys: 0.702 ± 0.032	1.939HisLeu: 1.939 ± 0.048	0.507HisMet: 0.507 ± 0.025	0.576HisAsn: 0.576 ± 0.026	1.092HisPro: 1.092 ± 0.038	0.595HisGln: 0.595 ± 0.025	1.038HisArg: 1.038 ± 0.036	0.989HisSer: 0.989 ± 0.035	1.139HisThr: 1.139 ± 0.039	1.312HisVal: 1.312 ± 0.041	0.201HisTrp: 0.201 ± 0.015	0.692HisTyr: 0.692 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.739IleAla: 5.739 ± 0.101	1.23IleCys: 1.23 ± 0.046	3.221IleAsp: 3.221 ± 0.066	3.03IleGlu: 3.03 ± 0.069	2.11IlePhe: 2.11 ± 0.065	4.584IleGly: 4.584 ± 0.09	1.228IleHis: 1.228 ± 0.038	3.105IleIle: 3.105 ± 0.078	2.116IleLys: 2.116 ± 0.06	6.164IleLeu: 6.164 ± 0.121	1.413IleMet: 1.413 ± 0.046	1.825IleAsn: 1.825 ± 0.053	3.111IlePro: 3.111 ± 0.063	1.961IleGln: 1.961 ± 0.049	3.383IleArg: 3.383 ± 0.068	3.87IleSer: 3.87 ± 0.067	3.472IleThr: 3.472 ± 0.068	3.983IleVal: 3.983 ± 0.09	0.591IleTrp: 0.591 ± 0.024	1.894IleTyr: 1.894 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	4.404LysAla: 4.404 ± 0.081	0.555LysCys: 0.555 ± 0.029	2.484LysAsp: 2.484 ± 0.065	3.57LysGlu: 3.57 ± 0.079	1.39LysPhe: 1.39 ± 0.044	3.182LysGly: 3.182 ± 0.073	0.803LysHis: 0.803 ± 0.032	2.753LysIle: 2.753 ± 0.065	3.357LysLys: 3.357 ± 0.087	4.473LysLeu: 4.473 ± 0.084	1.572LysMet: 1.572 ± 0.043	2.081LysAsn: 2.081 ± 0.058	1.919LysPro: 1.919 ± 0.053	1.753LysGln: 1.753 ± 0.049	2.786LysArg: 2.786 ± 0.057	2.471LysSer: 2.471 ± 0.066	3.025LysThr: 3.025 ± 0.064	3.109LysVal: 3.109 ± 0.069	0.521LysTrp: 0.521 ± 0.027	1.782LysTyr: 1.782 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	9.607LeuAla: 9.607 ± 0.119	2.038LeuCys: 2.038 ± 0.047	5.298LeuAsp: 5.298 ± 0.087	6.158LeuGlu: 6.158 ± 0.097	3.96LeuPhe: 3.96 ± 0.096	7.163LeuGly: 7.163 ± 0.129	1.824LeuHis: 1.824 ± 0.053	4.982LeuIle: 4.982 ± 0.094	4.587LeuLys: 4.587 ± 0.076	11.105LeuLeu: 11.105 ± 0.218	2.739LeuMet: 2.739 ± 0.069	3.159LeuAsn: 3.159 ± 0.071	4.811LeuPro: 4.811 ± 0.08	3.296LeuGln: 3.296 ± 0.074	5.802LeuArg: 5.802 ± 0.092	6.372LeuSer: 6.372 ± 0.101	6.257LeuThr: 6.257 ± 0.099	6.217LeuVal: 6.217 ± 0.101	1.048LeuTrp: 1.048 ± 0.043	3.163LeuTyr: 3.163 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	3.117MetAla: 3.117 ± 0.065	0.304MetCys: 0.304 ± 0.018	1.799MetAsp: 1.799 ± 0.052	2.425MetGlu: 2.425 ± 0.056	0.829MetPhe: 0.829 ± 0.034	2.234MetGly: 2.234 ± 0.056	0.429MetHis: 0.429 ± 0.023	1.467MetIle: 1.467 ± 0.047	1.861MetLys: 1.861 ± 0.045	2.714MetLeu: 2.714 ± 0.071	0.913MetMet: 0.913 ± 0.036	1.179MetAsn: 1.179 ± 0.035	1.244MetPro: 1.244 ± 0.041	0.974MetGln: 0.974 ± 0.035	1.526MetArg: 1.526 ± 0.048	1.707MetSer: 1.707 ± 0.049	1.774MetThr: 1.774 ± 0.053	2.025MetVal: 2.025 ± 0.053	0.204MetTrp: 0.204 ± 0.015	0.666MetTyr: 0.666 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.069AsnAla: 3.069 ± 0.066	0.597AsnCys: 0.597 ± 0.029	1.585AsnAsp: 1.585 ± 0.05	1.718AsnGlu: 1.718 ± 0.049	1.216AsnPhe: 1.216 ± 0.04	3.035AsnGly: 3.035 ± 0.08	0.666AsnHis: 0.666 ± 0.031	2.239AsnIle: 2.239 ± 0.054	1.289AsnLys: 1.289 ± 0.042	3.327AsnLeu: 3.327 ± 0.068	0.989AsnMet: 0.989 ± 0.038	1.088AsnAsn: 1.088 ± 0.04	1.914AsnPro: 1.914 ± 0.055	1.1AsnGln: 1.1 ± 0.042	1.983AsnArg: 1.983 ± 0.052	1.65AsnSer: 1.65 ± 0.045	2.0AsnThr: 2.0 ± 0.051	2.267AsnVal: 2.267 ± 0.058	0.392AsnTrp: 0.392 ± 0.023	1.203AsnTyr: 1.203 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	4.284ProAla: 4.284 ± 0.082	0.628ProCys: 0.628 ± 0.031	2.666ProAsp: 2.666 ± 0.061	3.807ProGlu: 3.807 ± 0.067	1.649ProPhe: 1.649 ± 0.038	3.256ProGly: 3.256 ± 0.066	0.713ProHis: 0.713 ± 0.026	2.169ProIle: 2.169 ± 0.046	1.807ProLys: 1.807 ± 0.05	3.504ProLeu: 3.504 ± 0.071	1.149ProMet: 1.149 ± 0.038	1.294ProAsn: 1.294 ± 0.044	1.368ProPro: 1.368 ± 0.05	1.376ProGln: 1.376 ± 0.042	1.665ProArg: 1.665 ± 0.05	2.142ProSer: 2.142 ± 0.066	2.017ProThr: 2.017 ± 0.05	3.55ProVal: 3.55 ± 0.078	0.419ProTrp: 0.419 ± 0.023	1.516ProTyr: 1.516 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.335GlnAla: 3.335 ± 0.075	0.429GlnCys: 0.429 ± 0.023	1.531GlnAsp: 1.531 ± 0.046	2.606GlnGlu: 2.606 ± 0.06	1.147GlnPhe: 1.147 ± 0.038	2.213GlnGly: 2.213 ± 0.057	0.622GlnHis: 0.622 ± 0.024	2.07GlnIle: 2.07 ± 0.055	2.011GlnLys: 2.011 ± 0.048	3.409GlnLeu: 3.409 ± 0.073	1.191GlnMet: 1.191 ± 0.044	1.339GlnAsn: 1.339 ± 0.043	1.257GlnPro: 1.257 ± 0.044	1.419GlnGln: 1.419 ± 0.04	1.992GlnArg: 1.992 ± 0.045	1.77GlnSer: 1.77 ± 0.049	1.831GlnThr: 1.831 ± 0.05	2.366GlnVal: 2.366 ± 0.057	0.373GlnTrp: 0.373 ± 0.023	1.249GlnTyr: 1.249 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	4.354ArgAla: 4.354 ± 0.082	0.931ArgCys: 0.931 ± 0.036	2.812ArgAsp: 2.812 ± 0.077	4.308ArgGlu: 4.308 ± 0.079	2.115ArgPhe: 2.115 ± 0.053	3.478ArgGly: 3.478 ± 0.062	1.096ArgHis: 1.096 ± 0.037	3.351ArgIle: 3.351 ± 0.064	2.93ArgLys: 2.93 ± 0.066	5.404ArgLeu: 5.404 ± 0.092	1.774ArgMet: 1.774 ± 0.049	1.873ArgAsn: 1.873 ± 0.058	2.084ArgPro: 2.084 ± 0.051	2.44ArgGln: 2.44 ± 0.062	3.744ArgArg: 3.744 ± 0.083	2.751ArgSer: 2.751 ± 0.067	2.798ArgThr: 2.798 ± 0.058	3.308ArgVal: 3.308 ± 0.064	0.615ArgTrp: 0.615 ± 0.03	1.918ArgTyr: 1.918 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	5.568SerAla: 5.568 ± 0.102	0.937SerCys: 0.937 ± 0.037	2.896SerAsp: 2.896 ± 0.076	3.118SerGlu: 3.118 ± 0.066	2.514SerPhe: 2.514 ± 0.057	5.456SerGly: 5.456 ± 0.092	1.122SerHis: 1.122 ± 0.04	3.249SerIle: 3.249 ± 0.055	2.362SerLys: 2.362 ± 0.066	5.311SerLeu: 5.311 ± 0.086	1.649SerMet: 1.649 ± 0.05	1.707SerAsn: 1.707 ± 0.043	2.207SerPro: 2.207 ± 0.053	1.734SerGln: 1.734 ± 0.048	3.017SerArg: 3.017 ± 0.063	3.217SerSer: 3.217 ± 0.083	2.854SerThr: 2.854 ± 0.069	4.227SerVal: 4.227 ± 0.065	0.576SerTrp: 0.576 ± 0.028	1.939SerTyr: 1.939 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	6.407ThrAla: 6.407 ± 0.12	0.817ThrCys: 0.817 ± 0.033	3.024ThrAsp: 3.024 ± 0.066	3.543ThrGlu: 3.543 ± 0.077	2.115ThrPhe: 2.115 ± 0.058	5.146ThrGly: 5.146 ± 0.09	1.003ThrHis: 1.003 ± 0.032	3.412ThrIle: 3.412 ± 0.067	2.152ThrLys: 2.152 ± 0.061	5.569ThrLeu: 5.569 ± 0.085	1.629ThrMet: 1.629 ± 0.042	1.592ThrAsn: 1.592 ± 0.05	2.892ThrPro: 2.892 ± 0.061	1.528ThrGln: 1.528 ± 0.046	2.435ThrArg: 2.435 ± 0.067	2.721ThrSer: 2.721 ± 0.064	2.871ThrThr: 2.871 ± 0.088	5.376ThrVal: 5.376 ± 0.121	0.554ThrTrp: 0.554 ± 0.03	2.015ThrTyr: 2.015 ± 0.071	0.0ThrXaa: 0.0 ± 0.0
Val	6.063ValAla: 6.063 ± 0.099	1.467ValCys: 1.467 ± 0.045	3.994ValAsp: 3.994 ± 0.076	4.697ValGlu: 4.697 ± 0.079	2.979ValPhe: 2.979 ± 0.049	5.099ValGly: 5.099 ± 0.095	1.175ValHis: 1.175 ± 0.041	4.306ValIle: 4.306 ± 0.088	3.286ValLys: 3.286 ± 0.066	7.581ValLeu: 7.581 ± 0.12	2.053ValMet: 2.053 ± 0.056	2.411ValAsn: 2.411 ± 0.054	3.142ValPro: 3.142 ± 0.061	2.17ValGln: 2.17 ± 0.053	3.728ValArg: 3.728 ± 0.072	4.754ValSer: 4.754 ± 0.076	4.623ValThr: 4.623 ± 0.127	5.313ValVal: 5.313 ± 0.089	0.767ValTrp: 0.767 ± 0.035	2.405ValTyr: 2.405 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.807TrpAla: 0.807 ± 0.035	0.212TrpCys: 0.212 ± 0.016	0.584TrpAsp: 0.584 ± 0.027	0.765TrpGlu: 0.765 ± 0.031	0.443TrpPhe: 0.443 ± 0.025	0.815TrpGly: 0.815 ± 0.027	0.182TrpHis: 0.182 ± 0.014	0.553TrpIle: 0.553 ± 0.028	0.587TrpLys: 0.587 ± 0.026	1.089TrpLeu: 1.089 ± 0.041	0.312TrpMet: 0.312 ± 0.021	0.486TrpAsn: 0.486 ± 0.023	0.323TrpPro: 0.323 ± 0.022	0.451TrpGln: 0.451 ± 0.022	0.549TrpArg: 0.549 ± 0.029	0.607TrpSer: 0.607 ± 0.029	0.538TrpThr: 0.538 ± 0.031	0.603TrpVal: 0.603 ± 0.024	0.113TrpTrp: 0.113 ± 0.013	0.419TrpTyr: 0.419 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.13TyrAla: 3.13 ± 0.069	0.64TyrCys: 0.64 ± 0.027	2.157TyrAsp: 2.157 ± 0.051	2.249TyrGlu: 2.249 ± 0.058	1.479TyrPhe: 1.479 ± 0.044	2.802TyrGly: 2.802 ± 0.064	0.76TyrHis: 0.76 ± 0.029	1.87TyrIle: 1.87 ± 0.05	1.26TyrLys: 1.26 ± 0.044	3.437TyrLeu: 3.437 ± 0.069	0.788TyrMet: 0.788 ± 0.033	1.216TyrAsn: 1.216 ± 0.04	1.4TyrPro: 1.4 ± 0.037	1.426TyrGln: 1.426 ± 0.048	2.0TyrArg: 2.0 ± 0.05	1.865TyrSer: 1.865 ± 0.05	2.139TyrThr: 2.139 ± 0.077	2.16TyrVal: 2.16 ± 0.051	0.356TyrTrp: 0.356 ± 0.022	1.334TyrTyr: 1.334 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2711 proteins (811945 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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