Oryctes borbonicus
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8820 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0T6BA91|A0A0T6BA91_9SCAR Uncharacterized protein (Fragment) OS=Oryctes borbonicus OX=1629725 GN=AMK59_1340 PE=4 SV=1
VV1 pKa = 5.79 EE2 pKa = 4.18 TADD5 pKa = 3.7 SLRR8 pKa = 11.84 ATWEE12 pKa = 3.85 APLNSEE18 pKa = 4.31 EE19 pKa = 4.78 CGVAYY24 pKa = 9.52 EE25 pKa = 4.24 VIYY28 pKa = 10.69 EE29 pKa = 3.92 NEE31 pKa = 4.06 ILGSSAVEE39 pKa = 4.25 VVGLAHH45 pKa = 7.27 DD46 pKa = 4.39 FNIEE50 pKa = 4.77 LIPCTDD56 pKa = 3.31 YY57 pKa = 11.24 TITVTPISFTGSKK70 pKa = 8.31 GTPVSQEE77 pKa = 3.63 VTPSIRR83 pKa = 11.84 VGSVNDD89 pKa = 4.93 LIAADD94 pKa = 3.82 VGDD97 pKa = 4.24 SVVLSWSTATVGVCPLVYY115 pKa = 9.9 IVNYY119 pKa = 9.96 AVNDD123 pKa = 3.7 VQGEE127 pKa = 4.14 EE128 pKa = 4.52 LEE130 pKa = 4.4 TTEE133 pKa = 5.14 VSVTFNRR140 pKa = 11.84 IPCSDD145 pKa = 3.37 SQFSVKK151 pKa = 10.13 VLSEE155 pKa = 4.55 GIVGPVAVANLEE167 pKa = 4.29 LGSADD172 pKa = 3.35 INSITQVEE180 pKa = 4.55 GLNLDD185 pKa = 3.61 GTILSWSPPVTVGLCEE201 pKa = 3.3 IVYY204 pKa = 10.32 IVDD207 pKa = 3.34 IEE209 pKa = 4.46 NTFGLQQTATRR220 pKa = 11.84 DD221 pKa = 3.46 LSIDD225 pKa = 3.99 LEE227 pKa = 4.86 LIPCSRR233 pKa = 11.84 NLFVVRR239 pKa = 11.84 GSANGITGEE248 pKa = 3.89 PATIEE253 pKa = 4.15 EE254 pKa = 4.45 YY255 pKa = 11.27 APIAEE260 pKa = 4.83 LAPPTHH266 pKa = 6.35 VRR268 pKa = 11.84 TKK270 pKa = 10.32 PAFTSADD277 pKa = 2.93 ITLQGQAISEE287 pKa = 4.31 NICRR291 pKa = 11.84 VEE293 pKa = 3.96 TAILEE298 pKa = 4.39 CSDD301 pKa = 2.89 ISGGVIEE308 pKa = 5.52 RR309 pKa = 11.84 EE310 pKa = 3.79 IEE312 pKa = 4.26 VMDD315 pKa = 3.4 NTPRR319 pKa = 11.84 IFTVLLDD326 pKa = 4.84 DD327 pKa = 5.54 LEE329 pKa = 4.67 QDD331 pKa = 3.81 TIYY334 pKa = 10.6 TCVARR339 pKa = 11.84 LEE341 pKa = 4.45 DD342 pKa = 3.58 SAAGNEE348 pKa = 4.25 FSFRR352 pKa = 11.84 TVPLVEE358 pKa = 4.65 KK359 pKa = 10.61 DD360 pKa = 4.53 LIISDD365 pKa = 3.33 ITEE368 pKa = 4.11 GGFLVSWIEE377 pKa = 4.39 DD378 pKa = 3.33 YY379 pKa = 11.16 GQQFVIGYY387 pKa = 7.75 EE388 pKa = 3.91 LTIEE392 pKa = 4.35 SLGPAHH398 pKa = 7.37 RR399 pKa = 11.84 VPDD402 pKa = 4.12 GCSVDD407 pKa = 3.69 TTVTTVSLSNEE418 pKa = 4.07 EE419 pKa = 4.06 LSSAFVGGLPDD430 pKa = 3.18 YY431 pKa = 10.5 AYY433 pKa = 10.21 RR434 pKa = 11.84 VVLTTYY440 pKa = 8.4 YY441 pKa = 10.66 TLGLSLQSNTASARR455 pKa = 11.84 TLAGVPSLPEE465 pKa = 3.7 NLVLDD470 pKa = 4.68 FVSGSTEE477 pKa = 3.91 AYY479 pKa = 9.01 NVSAILSWSSPCEE492 pKa = 3.78 LNGGLRR498 pKa = 11.84 GFGFNITARR507 pKa = 11.84 FSLGGVEE514 pKa = 4.84 QSVTVNLGVDD524 pKa = 3.64 EE525 pKa = 5.64 DD526 pKa = 4.6 DD527 pKa = 3.93 VNAVISKK534 pKa = 8.8 TITDD538 pKa = 3.55 INPFFRR544 pKa = 11.84 YY545 pKa = 8.61 EE546 pKa = 3.79 VSVNVIAADD555 pKa = 4.05 DD556 pKa = 4.48 GPNAVLGFIPPAACVCDD573 pKa = 3.59 AAA575 pKa = 5.95
Molecular weight: 61.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.643
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.088
Thurlkill 3.668
EMBOSS 3.706
Sillero 3.948
Patrickios 0.731
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A0T6B3U6|A0A0T6B3U6_9SCAR LIM domain containing protein OS=Oryctes borbonicus OX=1629725 GN=AMK59_6067 PE=4 SV=1
RR1 pKa = 7.08 NGGRR5 pKa = 11.84 AKK7 pKa = 10.35 HH8 pKa = 5.76 GRR10 pKa = 11.84 GHH12 pKa = 5.99 VKK14 pKa = 10.0 PVRR17 pKa = 11.84 CTNCARR23 pKa = 11.84 CVPKK27 pKa = 10.77 DD28 pKa = 3.35 KK29 pKa = 10.79 AIKK32 pKa = 10.32 KK33 pKa = 8.63 FVIRR37 pKa = 11.84 NIVEE41 pKa = 3.73 AAAVRR46 pKa = 11.84 DD47 pKa = 3.62 ITDD50 pKa = 3.15 ASVYY54 pKa = 9.41 TSYY57 pKa = 11.64 VLPKK61 pKa = 10.2 LYY63 pKa = 10.77 AKK65 pKa = 10.42 LHH67 pKa = 5.53 YY68 pKa = 10.0 CVSCAIHH75 pKa = 6.27 SKK77 pKa = 8.47 VVRR80 pKa = 11.84 NRR82 pKa = 11.84 SKK84 pKa = 11.01 EE85 pKa = 3.92 EE86 pKa = 3.78 RR87 pKa = 11.84 KK88 pKa = 10.18 KK89 pKa = 9.95 RR90 pKa = 11.84 TPPARR95 pKa = 11.84 NFPGRR100 pKa = 11.84 DD101 pKa = 3.11 NVRR104 pKa = 11.84 VVQQ107 pKa = 3.84
Molecular weight: 12.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.78
IPC_protein 10.409
Toseland 10.818
ProMoST 10.599
Dawson 10.891
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.242
Grimsley 10.935
Solomon 10.994
Lehninger 10.965
Nozaki 10.804
DTASelect 10.555
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.818
Patrickios 10.965
IPC_peptide 11.008
IPC2_peptide 9.677
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8820
0
8820
3587004
99
6462
406.7
46.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.598 ± 0.025
2.059 ± 0.029
5.564 ± 0.02
6.818 ± 0.037
3.856 ± 0.022
5.195 ± 0.037
2.424 ± 0.012
6.284 ± 0.021
7.037 ± 0.036
9.127 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.237 ± 0.013
5.566 ± 0.024
4.946 ± 0.035
4.227 ± 0.025
4.977 ± 0.024
7.821 ± 0.034
5.882 ± 0.021
5.996 ± 0.022
1.018 ± 0.009
3.368 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here