Blautia sp. YL58

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C7I788|A0A1C7I788_9FIRM Uroporphyrinogen_deCOase domain-containing protein OS=Blautia sp. YL58 OX=1796616 GN=A4V09_03225 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84AEE4 pKa = 3.83TGIRR8 pKa = 11.84ADD10 pKa = 4.57CNTSTNVIDD19 pKa = 5.49LLMANKK25 pKa = 10.16SPVEE29 pKa = 3.56ICGIINKK36 pKa = 9.36LLNCLLFDD44 pKa = 5.17GIEE47 pKa = 4.19VQDD50 pKa = 4.1GDD52 pKa = 4.36NPGDD56 pKa = 3.64GCLDD60 pKa = 4.08YY61 pKa = 10.28ITYY64 pKa = 10.81NPDD67 pKa = 3.6DD68 pKa = 3.8DD69 pKa = 4.07TLYY72 pKa = 11.0FFTKK76 pKa = 10.56DD77 pKa = 2.99LGRR80 pKa = 5.45

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C7IDT7|A0A1C7IDT7_9FIRM Stage 0 sporulation protein A homolog OS=Blautia sp. YL58 OX=1796616 GN=A4V09_19985 PE=4 SV=2
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.26AGRR28 pKa = 11.84KK29 pKa = 8.22VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.38GRR39 pKa = 11.84KK40 pKa = 8.94RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4518

0

4518

1457493

29

3477

322.6

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.367 ± 0.04

1.625 ± 0.016

5.44 ± 0.034

7.615 ± 0.045

4.253 ± 0.029

7.337 ± 0.033

1.802 ± 0.017

7.09 ± 0.038

6.697 ± 0.028

9.104 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.261 ± 0.02

4.104 ± 0.03

3.432 ± 0.02

3.272 ± 0.021

4.601 ± 0.03

5.743 ± 0.025

5.224 ± 0.024

6.813 ± 0.034

1.043 ± 0.017

4.178 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski