Muribaculum sp. An287
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2021 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4CFU6|A0A1Y4CFU6_9BACT TPM_phosphatase domain-containing protein OS=Muribaculum sp. An287 OX=1965623 GN=B5F81_05730 PE=4 SV=1
MM1 pKa = 7.2 KK2 pKa = 10.35 RR3 pKa = 11.84 ILIFMLSCVSAAAIIGCEE21 pKa = 3.98 KK22 pKa = 10.37 QIEE25 pKa = 4.48 SEE27 pKa = 4.32 PPVLSSDD34 pKa = 3.26 AKK36 pKa = 10.46 DD37 pKa = 3.11 IHH39 pKa = 5.69 VTAEE43 pKa = 4.26 GGTQTITYY51 pKa = 9.54 NIEE54 pKa = 3.68 NPAEE58 pKa = 4.44 DD59 pKa = 4.14 GMITALPDD67 pKa = 4.5 SEE69 pKa = 5.25 CDD71 pKa = 3.22 WIKK74 pKa = 9.94 ITDD77 pKa = 3.88 TEE79 pKa = 4.43 TSGVITFNVDD89 pKa = 3.52 KK90 pKa = 11.34 NEE92 pKa = 3.89 TDD94 pKa = 3.29 AKK96 pKa = 9.97 RR97 pKa = 11.84 EE98 pKa = 4.06 SKK100 pKa = 9.06 ITVTYY105 pKa = 9.14 EE106 pKa = 3.72 YY107 pKa = 11.06 SGGDD111 pKa = 3.41 PQSFSVNVSQDD122 pKa = 3.15 SGEE125 pKa = 4.34 NNPDD129 pKa = 4.17 PDD131 pKa = 5.27 PDD133 pKa = 5.0 PDD135 pKa = 5.1 PDD137 pKa = 4.62 PDD139 pKa = 4.38 PNPGEE144 pKa = 4.22 DD145 pKa = 3.26 VFTITVDD152 pKa = 3.32 NITYY156 pKa = 7.96 STAHH160 pKa = 6.22 IRR162 pKa = 11.84 VVPDD166 pKa = 4.14 DD167 pKa = 3.52 EE168 pKa = 4.41 TMPYY172 pKa = 9.48 IVYY175 pKa = 10.47 VDD177 pKa = 3.56 MASEE181 pKa = 4.15 IKK183 pKa = 10.49 DD184 pKa = 3.67 LSDD187 pKa = 5.03 DD188 pKa = 3.87 EE189 pKa = 4.67 VFQMDD194 pKa = 3.65 YY195 pKa = 11.24 DD196 pKa = 4.28 IIVGNANASQITINEE211 pKa = 4.11 YY212 pKa = 10.61 LNTIVKK218 pKa = 10.08 HH219 pKa = 6.0 GPQEE223 pKa = 4.21 TTQKK227 pKa = 10.86 SLNPGNEE234 pKa = 4.04 YY235 pKa = 10.9 AVWAYY240 pKa = 10.45 GIDD243 pKa = 3.72 LQEE246 pKa = 5.41 DD247 pKa = 4.04 GTLVRR252 pKa = 11.84 LSKK255 pKa = 9.49 ITRR258 pKa = 11.84 EE259 pKa = 4.06 YY260 pKa = 9.42 FTTEE264 pKa = 3.45 IPPVTEE270 pKa = 3.62 NSITLEE276 pKa = 4.09 VEE278 pKa = 4.2 STGTTLSAVATPYY291 pKa = 11.04 TNDD294 pKa = 3.31 KK295 pKa = 10.77 YY296 pKa = 11.98 YY297 pKa = 11.04 NLNWNNEE304 pKa = 4.16 STLVNAGYY312 pKa = 9.14 TDD314 pKa = 3.43 GTVAEE319 pKa = 4.39 RR320 pKa = 11.84 CFQFEE325 pKa = 4.13 YY326 pKa = 10.7 DD327 pKa = 3.9 YY328 pKa = 9.9 MIKK331 pKa = 10.51 YY332 pKa = 9.9 ISFYY336 pKa = 11.39 AVTDD340 pKa = 3.64 WANVKK345 pKa = 10.01 QGPATTSTVMTKK357 pKa = 10.28 PDD359 pKa = 3.34 TYY361 pKa = 11.57 YY362 pKa = 11.29 LFAYY366 pKa = 8.6 FINNDD371 pKa = 2.78 ATADD375 pKa = 3.7 GEE377 pKa = 4.64 IILKK381 pKa = 9.33 TIEE384 pKa = 3.75 FDD386 pKa = 3.53 GANVTIRR393 pKa = 3.51
Molecular weight: 43.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.706
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.732
EMBOSS 3.808
Sillero 4.024
Patrickios 1.113
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A1Y4CL05|A0A1Y4CL05_9BACT Uncharacterized protein OS=Muribaculum sp. An287 OX=1965623 GN=B5F81_02195 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 4.06 YY3 pKa = 10.44 SINKK7 pKa = 9.56 GIGRR11 pKa = 11.84 SVEE14 pKa = 4.22 FKK16 pKa = 10.81 GLKK19 pKa = 9.21 AQYY22 pKa = 11.01 LFIFCGGLLAVFVLFMVMYY41 pKa = 8.69 MAGVAQGVCVGFGAVSASALIWLTFRR67 pKa = 11.84 LNARR71 pKa = 11.84 YY72 pKa = 9.82 GEE74 pKa = 4.32 HH75 pKa = 7.16 GLMKK79 pKa = 10.59 LGALRR84 pKa = 11.84 SRR86 pKa = 11.84 PRR88 pKa = 11.84 YY89 pKa = 8.8 IINRR93 pKa = 11.84 RR94 pKa = 11.84 AVFRR98 pKa = 11.84 LFKK101 pKa = 9.99 QTDD104 pKa = 3.19 RR105 pKa = 11.84 KK106 pKa = 9.94 EE107 pKa = 4.35 AIRR110 pKa = 4.45
Molecular weight: 12.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.955
IPC_protein 10.774
Toseland 10.701
ProMoST 10.687
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 11.052
Grimsley 10.891
Solomon 10.935
Lehninger 10.906
Nozaki 10.687
DTASelect 10.555
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.76
Patrickios 10.789
IPC_peptide 10.935
IPC2_peptide 9.663
IPC2.peptide.svr19 8.325
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2021
0
2021
695823
38
1912
344.3
38.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.922 ± 0.056
1.332 ± 0.021
5.978 ± 0.037
6.887 ± 0.054
4.566 ± 0.037
7.661 ± 0.044
1.611 ± 0.024
6.947 ± 0.051
5.858 ± 0.049
8.554 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.887 ± 0.027
4.267 ± 0.036
3.809 ± 0.032
2.519 ± 0.03
5.202 ± 0.046
6.744 ± 0.048
5.187 ± 0.042
6.783 ± 0.041
1.066 ± 0.02
4.22 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here