Muribaculum sp. An287

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; Muribaculum; unclassified Muribaculum

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y4CFU6|A0A1Y4CFU6_9BACT TPM_phosphatase domain-containing protein OS=Muribaculum sp. An287 OX=1965623 GN=B5F81_05730 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.35RR3 pKa = 11.84ILIFMLSCVSAAAIIGCEE21 pKa = 3.98KK22 pKa = 10.37QIEE25 pKa = 4.48SEE27 pKa = 4.32PPVLSSDD34 pKa = 3.26AKK36 pKa = 10.46DD37 pKa = 3.11IHH39 pKa = 5.69VTAEE43 pKa = 4.26GGTQTITYY51 pKa = 9.54NIEE54 pKa = 3.68NPAEE58 pKa = 4.44DD59 pKa = 4.14GMITALPDD67 pKa = 4.5SEE69 pKa = 5.25CDD71 pKa = 3.22WIKK74 pKa = 9.94ITDD77 pKa = 3.88TEE79 pKa = 4.43TSGVITFNVDD89 pKa = 3.52KK90 pKa = 11.34NEE92 pKa = 3.89TDD94 pKa = 3.29AKK96 pKa = 9.97RR97 pKa = 11.84EE98 pKa = 4.06SKK100 pKa = 9.06ITVTYY105 pKa = 9.14EE106 pKa = 3.72YY107 pKa = 11.06SGGDD111 pKa = 3.41PQSFSVNVSQDD122 pKa = 3.15SGEE125 pKa = 4.34NNPDD129 pKa = 4.17PDD131 pKa = 5.27PDD133 pKa = 5.0PDD135 pKa = 5.1PDD137 pKa = 4.62PDD139 pKa = 4.38PNPGEE144 pKa = 4.22DD145 pKa = 3.26VFTITVDD152 pKa = 3.32NITYY156 pKa = 7.96STAHH160 pKa = 6.22IRR162 pKa = 11.84VVPDD166 pKa = 4.14DD167 pKa = 3.52EE168 pKa = 4.41TMPYY172 pKa = 9.48IVYY175 pKa = 10.47VDD177 pKa = 3.56MASEE181 pKa = 4.15IKK183 pKa = 10.49DD184 pKa = 3.67LSDD187 pKa = 5.03DD188 pKa = 3.87EE189 pKa = 4.67VFQMDD194 pKa = 3.65YY195 pKa = 11.24DD196 pKa = 4.28IIVGNANASQITINEE211 pKa = 4.11YY212 pKa = 10.61LNTIVKK218 pKa = 10.08HH219 pKa = 6.0GPQEE223 pKa = 4.21TTQKK227 pKa = 10.86SLNPGNEE234 pKa = 4.04YY235 pKa = 10.9AVWAYY240 pKa = 10.45GIDD243 pKa = 3.72LQEE246 pKa = 5.41DD247 pKa = 4.04GTLVRR252 pKa = 11.84LSKK255 pKa = 9.49ITRR258 pKa = 11.84EE259 pKa = 4.06YY260 pKa = 9.42FTTEE264 pKa = 3.45IPPVTEE270 pKa = 3.62NSITLEE276 pKa = 4.09VEE278 pKa = 4.2STGTTLSAVATPYY291 pKa = 11.04TNDD294 pKa = 3.31KK295 pKa = 10.77YY296 pKa = 11.98YY297 pKa = 11.04NLNWNNEE304 pKa = 4.16STLVNAGYY312 pKa = 9.14TDD314 pKa = 3.43GTVAEE319 pKa = 4.39RR320 pKa = 11.84CFQFEE325 pKa = 4.13YY326 pKa = 10.7DD327 pKa = 3.9YY328 pKa = 9.9MIKK331 pKa = 10.51YY332 pKa = 9.9ISFYY336 pKa = 11.39AVTDD340 pKa = 3.64WANVKK345 pKa = 10.01QGPATTSTVMTKK357 pKa = 10.28PDD359 pKa = 3.34TYY361 pKa = 11.57YY362 pKa = 11.29LFAYY366 pKa = 8.6FINNDD371 pKa = 2.78ATADD375 pKa = 3.7GEE377 pKa = 4.64IILKK381 pKa = 9.33TIEE384 pKa = 3.75FDD386 pKa = 3.53GANVTIRR393 pKa = 3.51

Molecular weight:
43.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y4CL05|A0A1Y4CL05_9BACT Uncharacterized protein OS=Muribaculum sp. An287 OX=1965623 GN=B5F81_02195 PE=4 SV=1
MM1 pKa = 7.78EE2 pKa = 4.06YY3 pKa = 10.44SINKK7 pKa = 9.56GIGRR11 pKa = 11.84SVEE14 pKa = 4.22FKK16 pKa = 10.81GLKK19 pKa = 9.21AQYY22 pKa = 11.01LFIFCGGLLAVFVLFMVMYY41 pKa = 8.69MAGVAQGVCVGFGAVSASALIWLTFRR67 pKa = 11.84LNARR71 pKa = 11.84YY72 pKa = 9.82GEE74 pKa = 4.32HH75 pKa = 7.16GLMKK79 pKa = 10.59LGALRR84 pKa = 11.84SRR86 pKa = 11.84PRR88 pKa = 11.84YY89 pKa = 8.8IINRR93 pKa = 11.84RR94 pKa = 11.84AVFRR98 pKa = 11.84LFKK101 pKa = 9.99QTDD104 pKa = 3.19RR105 pKa = 11.84KK106 pKa = 9.94EE107 pKa = 4.35AIRR110 pKa = 4.45

Molecular weight:
12.43 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2021

0

2021

695823

38

1912

344.3

38.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.922 ± 0.056

1.332 ± 0.021

5.978 ± 0.037

6.887 ± 0.054

4.566 ± 0.037

7.661 ± 0.044

1.611 ± 0.024

6.947 ± 0.051

5.858 ± 0.049

8.554 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.027

4.267 ± 0.036

3.809 ± 0.032

2.519 ± 0.03

5.202 ± 0.046

6.744 ± 0.048

5.187 ± 0.042

6.783 ± 0.041

1.066 ± 0.02

4.22 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski