Streptomyces phage Itza

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; unclassified Arquatrovirinae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SZF2|A0A6M3SZF2_9CAUD Uncharacterized protein OS=Streptomyces phage Itza OX=2725627 GN=25 PE=4 SV=1
MM1 pKa = 7.76ALNDD5 pKa = 4.23DD6 pKa = 3.85ATLVIGSGNYY16 pKa = 7.78LTAPAGTPIPEE27 pKa = 4.97DD28 pKa = 3.74LLSPASPWEE37 pKa = 4.29AVGHH41 pKa = 5.85TSLEE45 pKa = 4.53DD46 pKa = 3.06IFGITSEE53 pKa = 4.22GGEE56 pKa = 3.92ATVIGTLQNKK66 pKa = 7.42SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.2TMTFTLQQFDD87 pKa = 3.87APGLRR92 pKa = 11.84LYY94 pKa = 10.82YY95 pKa = 10.24GANAPTLPDD104 pKa = 3.54GSIGVPTEE112 pKa = 4.05PEE114 pKa = 3.85PTVCAFLAIFVDD126 pKa = 4.23GDD128 pKa = 3.48NHH130 pKa = 6.56FAFYY134 pKa = 10.71APKK137 pKa = 10.65AEE139 pKa = 4.56IYY141 pKa = 10.28RR142 pKa = 11.84ADD144 pKa = 4.92DD145 pKa = 3.37MTLSDD150 pKa = 4.12TEE152 pKa = 4.4SLAGLPLGVKK162 pKa = 10.09PMAYY166 pKa = 10.07SSNPWTYY173 pKa = 11.07AVTPLGSSIAATGATAGIPGAFTPTGATPPADD205 pKa = 3.95LEE207 pKa = 4.22ALQGSTIVASPGTAWTEE224 pKa = 4.04GQYY227 pKa = 10.81VALGDD232 pKa = 3.83LTSAHH237 pKa = 7.06WDD239 pKa = 3.3GDD241 pKa = 3.61SWEE244 pKa = 4.41TGAAPAA250 pKa = 4.82

Molecular weight: 25.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SZD3|A0A6M3SZD3_9CAUD Terminase small subunit OS=Streptomyces phage Itza OX=2725627 GN=5 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 9.88ILKK5 pKa = 8.5WVAVVVAALTLAVSSGAGTADD26 pKa = 3.71AAPKK30 pKa = 8.52PTYY33 pKa = 9.92KK34 pKa = 9.93PCRR37 pKa = 11.84YY38 pKa = 8.75EE39 pKa = 5.11DD40 pKa = 3.9SPGPCYY46 pKa = 9.84WDD48 pKa = 3.56AGSFGNGRR56 pKa = 11.84GHH58 pKa = 6.41SFYY61 pKa = 10.33VTPTQRR67 pKa = 11.84VRR69 pKa = 11.84YY70 pKa = 9.24VRR72 pKa = 11.84DD73 pKa = 3.34LVVVRR78 pKa = 11.84GWHH81 pKa = 5.97AAPNSLRR88 pKa = 11.84ARR90 pKa = 11.84QHH92 pKa = 4.58GRR94 pKa = 11.84KK95 pKa = 7.59VTALIRR101 pKa = 11.84PAWHH105 pKa = 7.04KK106 pKa = 11.2APASLVNEE114 pKa = 5.29LIRR117 pKa = 11.84DD118 pKa = 3.88GWRR121 pKa = 11.84GVNGKK126 pKa = 9.98RR127 pKa = 11.84IQRR130 pKa = 11.84SDD132 pKa = 4.24RR133 pKa = 11.84GHH135 pKa = 5.28CWEE138 pKa = 5.29RR139 pKa = 11.84IGDD142 pKa = 3.73TSYY145 pKa = 11.21LVCRR149 pKa = 11.84SGFFEE154 pKa = 4.4TSS156 pKa = 2.73

Molecular weight: 17.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15252

31

1161

190.7

20.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.172 ± 0.316

0.721 ± 0.12

6.878 ± 0.265

7.002 ± 0.466

2.911 ± 0.141

8.491 ± 0.287

2.019 ± 0.184

4.183 ± 0.209

3.901 ± 0.222

8.438 ± 0.435

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.134

2.76 ± 0.152

5.009 ± 0.263

3.219 ± 0.147

7.068 ± 0.448

5.901 ± 0.297

6.373 ± 0.281

7.245 ± 0.237

1.882 ± 0.131

2.754 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski