Amino acid dipepetide frequency for Streptomyces phage Itza

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.671AlaAla: 15.671 ± 1.286
0.393AlaCys: 0.393 ± 0.156
6.95AlaAsp: 6.95 ± 0.673
7.737AlaGlu: 7.737 ± 0.839
2.819AlaPhe: 2.819 ± 0.45
7.344AlaGly: 7.344 ± 0.94
2.033AlaHis: 2.033 ± 0.421
3.672AlaIle: 3.672 ± 0.637
4.655AlaLys: 4.655 ± 0.632
10.36AlaLeu: 10.36 ± 1.075
3.606AlaMet: 3.606 ± 0.437
2.819AlaAsn: 2.819 ± 0.441
5.049AlaPro: 5.049 ± 0.841
3.41AlaGln: 3.41 ± 0.525
7.672AlaArg: 7.672 ± 0.821
5.836AlaSer: 5.836 ± 0.758
6.491AlaThr: 6.491 ± 0.718
9.508AlaVal: 9.508 ± 0.821
2.492AlaTrp: 2.492 ± 0.384
3.213AlaTyr: 3.213 ± 0.477
0.0AlaXaa: 0.0 ± 0.0
Cys
0.525CysAla: 0.525 ± 0.238
0.066CysCys: 0.066 ± 0.06
0.525CysAsp: 0.525 ± 0.185
0.393CysGlu: 0.393 ± 0.165
0.262CysPhe: 0.262 ± 0.121
0.984CysGly: 0.984 ± 0.287
0.197CysHis: 0.197 ± 0.114
0.328CysIle: 0.328 ± 0.117
0.131CysLys: 0.131 ± 0.093
0.525CysLeu: 0.525 ± 0.228
0.066CysMet: 0.066 ± 0.065
0.131CysAsn: 0.131 ± 0.096
0.262CysPro: 0.262 ± 0.107
0.328CysGln: 0.328 ± 0.132
1.115CysArg: 1.115 ± 0.292
0.459CysSer: 0.459 ± 0.195
0.197CysThr: 0.197 ± 0.127
0.197CysVal: 0.197 ± 0.101
0.131CysTrp: 0.131 ± 0.097
0.393CysTyr: 0.393 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
7.344AspAla: 7.344 ± 0.73
0.393AspCys: 0.393 ± 0.155
6.098AspAsp: 6.098 ± 0.681
6.098AspGlu: 6.098 ± 0.891
1.836AspPhe: 1.836 ± 0.361
6.95AspGly: 6.95 ± 0.786
1.705AspHis: 1.705 ± 0.328
2.951AspIle: 2.951 ± 0.416
2.492AspLys: 2.492 ± 0.407
6.229AspLeu: 6.229 ± 0.547
1.836AspMet: 1.836 ± 0.379
1.508AspAsn: 1.508 ± 0.264
2.819AspPro: 2.819 ± 0.431
2.098AspGln: 2.098 ± 0.333
3.41AspArg: 3.41 ± 0.489
3.934AspSer: 3.934 ± 0.421
3.869AspThr: 3.869 ± 0.56
3.934AspVal: 3.934 ± 0.503
1.639AspTrp: 1.639 ± 0.335
1.639AspTyr: 1.639 ± 0.264
0.0AspXaa: 0.0 ± 0.0
Glu
8.458GluAla: 8.458 ± 0.783
0.918GluCys: 0.918 ± 0.299
5.246GluAsp: 5.246 ± 0.685
4.459GluGlu: 4.459 ± 0.722
2.098GluPhe: 2.098 ± 0.312
6.098GluGly: 6.098 ± 0.761
1.77GluHis: 1.77 ± 0.378
2.819GluIle: 2.819 ± 0.435
2.164GluLys: 2.164 ± 0.392
6.754GluLeu: 6.754 ± 0.818
1.574GluMet: 1.574 ± 0.274
1.311GluAsn: 1.311 ± 0.261
2.688GluPro: 2.688 ± 0.455
2.885GluGln: 2.885 ± 0.461
5.311GluArg: 5.311 ± 0.85
3.869GluSer: 3.869 ± 0.499
3.606GluThr: 3.606 ± 0.67
4.787GluVal: 4.787 ± 0.634
0.984GluTrp: 0.984 ± 0.243
2.229GluTyr: 2.229 ± 0.388
0.0GluXaa: 0.0 ± 0.0
Phe
2.819PheAla: 2.819 ± 0.423
0.131PheCys: 0.131 ± 0.08
3.213PheAsp: 3.213 ± 0.505
2.229PheGlu: 2.229 ± 0.489
0.787PhePhe: 0.787 ± 0.171
2.557PheGly: 2.557 ± 0.415
0.656PheHis: 0.656 ± 0.205
1.246PheIle: 1.246 ± 0.331
0.984PheLys: 0.984 ± 0.244
1.902PheLeu: 1.902 ± 0.373
0.393PheMet: 0.393 ± 0.158
0.721PheAsn: 0.721 ± 0.279
1.508PhePro: 1.508 ± 0.29
1.246PheGln: 1.246 ± 0.288
1.705PheArg: 1.705 ± 0.389
1.443PheSer: 1.443 ± 0.367
2.098PheThr: 2.098 ± 0.347
2.164PheVal: 2.164 ± 0.383
0.459PheTrp: 0.459 ± 0.155
0.852PheTyr: 0.852 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
7.606GlyAla: 7.606 ± 0.848
0.459GlyCys: 0.459 ± 0.254
5.442GlyAsp: 5.442 ± 0.683
5.442GlyGlu: 5.442 ± 0.645
2.951GlyPhe: 2.951 ± 0.47
7.016GlyGly: 7.016 ± 0.707
1.967GlyHis: 1.967 ± 0.383
3.082GlyIle: 3.082 ± 0.45
4.065GlyLys: 4.065 ± 0.506
7.475GlyLeu: 7.475 ± 1.003
1.246GlyMet: 1.246 ± 0.27
2.361GlyAsn: 2.361 ± 0.381
4.065GlyPro: 4.065 ± 0.701
3.082GlyGln: 3.082 ± 0.424
5.967GlyArg: 5.967 ± 0.712
5.18GlySer: 5.18 ± 0.682
5.573GlyThr: 5.573 ± 0.597
6.426GlyVal: 6.426 ± 0.514
2.688GlyTrp: 2.688 ± 0.485
2.754GlyTyr: 2.754 ± 0.607
0.0GlyXaa: 0.0 ± 0.0
His
2.033HisAla: 2.033 ± 0.358
0.328HisCys: 0.328 ± 0.144
1.443HisAsp: 1.443 ± 0.29
1.311HisGlu: 1.311 ± 0.292
0.656HisPhe: 0.656 ± 0.182
2.033HisGly: 2.033 ± 0.396
0.59HisHis: 0.59 ± 0.179
1.049HisIle: 1.049 ± 0.227
0.197HisLys: 0.197 ± 0.098
1.902HisLeu: 1.902 ± 0.325
0.328HisMet: 0.328 ± 0.149
0.328HisAsn: 0.328 ± 0.12
1.049HisPro: 1.049 ± 0.255
0.393HisGln: 0.393 ± 0.149
1.246HisArg: 1.246 ± 0.312
1.246HisSer: 1.246 ± 0.344
1.443HisThr: 1.443 ± 0.308
1.246HisVal: 1.246 ± 0.335
0.656HisTrp: 0.656 ± 0.203
0.721HisTyr: 0.721 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
3.934IleAla: 3.934 ± 0.484
0.262IleCys: 0.262 ± 0.127
3.606IleAsp: 3.606 ± 0.404
4.328IleGlu: 4.328 ± 0.501
1.377IlePhe: 1.377 ± 0.305
3.213IleGly: 3.213 ± 0.578
0.525IleHis: 0.525 ± 0.207
1.443IleIle: 1.443 ± 0.493
1.705IleLys: 1.705 ± 0.321
2.688IleLeu: 2.688 ± 0.634
0.59IleMet: 0.59 ± 0.174
1.377IleAsn: 1.377 ± 0.343
2.229IlePro: 2.229 ± 0.355
1.18IleGln: 1.18 ± 0.352
3.016IleArg: 3.016 ± 0.335
2.885IleSer: 2.885 ± 0.5
2.623IleThr: 2.623 ± 0.323
3.344IleVal: 3.344 ± 0.536
0.59IleTrp: 0.59 ± 0.158
0.918IleTyr: 0.918 ± 0.207
0.0IleXaa: 0.0 ± 0.0
Lys
3.934LysAla: 3.934 ± 0.633
0.262LysCys: 0.262 ± 0.175
2.819LysAsp: 2.819 ± 0.432
1.049LysGlu: 1.049 ± 0.229
0.852LysPhe: 0.852 ± 0.241
3.869LysGly: 3.869 ± 0.539
0.787LysHis: 0.787 ± 0.204
1.836LysIle: 1.836 ± 0.325
2.426LysLys: 2.426 ± 0.46
3.344LysLeu: 3.344 ± 0.476
0.787LysMet: 0.787 ± 0.271
1.18LysAsn: 1.18 ± 0.355
2.819LysPro: 2.819 ± 0.638
0.984LysGln: 0.984 ± 0.188
2.819LysArg: 2.819 ± 0.498
2.033LysSer: 2.033 ± 0.446
2.361LysThr: 2.361 ± 0.37
3.082LysVal: 3.082 ± 0.542
0.525LysTrp: 0.525 ± 0.197
1.246LysTyr: 1.246 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
9.376LeuAla: 9.376 ± 0.899
0.393LeuCys: 0.393 ± 0.147
6.491LeuAsp: 6.491 ± 0.692
5.049LeuGlu: 5.049 ± 0.562
1.311LeuPhe: 1.311 ± 0.316
6.95LeuGly: 6.95 ± 1.089
1.77LeuHis: 1.77 ± 0.366
3.934LeuIle: 3.934 ± 0.317
3.541LeuLys: 3.541 ± 0.563
6.623LeuLeu: 6.623 ± 0.756
1.902LeuMet: 1.902 ± 0.349
3.082LeuAsn: 3.082 ± 0.443
4.983LeuPro: 4.983 ± 0.548
2.361LeuGln: 2.361 ± 0.407
5.705LeuArg: 5.705 ± 0.781
5.639LeuSer: 5.639 ± 0.645
5.705LeuThr: 5.705 ± 0.659
6.426LeuVal: 6.426 ± 0.838
1.049LeuTrp: 1.049 ± 0.269
2.098LeuTyr: 2.098 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
3.278MetAla: 3.278 ± 0.43
0.0MetCys: 0.0 ± 0.0
0.656MetAsp: 0.656 ± 0.212
0.656MetGlu: 0.656 ± 0.231
0.459MetPhe: 0.459 ± 0.257
1.836MetGly: 1.836 ± 0.507
0.328MetHis: 0.328 ± 0.168
0.656MetIle: 0.656 ± 0.19
1.049MetLys: 1.049 ± 0.264
1.443MetLeu: 1.443 ± 0.315
0.328MetMet: 0.328 ± 0.136
0.918MetAsn: 0.918 ± 0.219
0.984MetPro: 0.984 ± 0.256
0.787MetGln: 0.787 ± 0.197
1.18MetArg: 1.18 ± 0.329
2.098MetSer: 2.098 ± 0.343
2.229MetThr: 2.229 ± 0.371
1.246MetVal: 1.246 ± 0.323
0.131MetTrp: 0.131 ± 0.099
0.459MetTyr: 0.459 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
2.623AsnAla: 2.623 ± 0.404
0.197AsnCys: 0.197 ± 0.111
1.115AsnAsp: 1.115 ± 0.251
2.426AsnGlu: 2.426 ± 0.332
0.852AsnPhe: 0.852 ± 0.276
3.278AsnGly: 3.278 ± 0.377
0.525AsnHis: 0.525 ± 0.199
0.984AsnIle: 0.984 ± 0.232
1.246AsnLys: 1.246 ± 0.255
2.295AsnLeu: 2.295 ± 0.391
0.393AsnMet: 0.393 ± 0.17
0.656AsnAsn: 0.656 ± 0.219
1.967AsnPro: 1.967 ± 0.38
0.59AsnGln: 0.59 ± 0.182
2.098AsnArg: 2.098 ± 0.317
1.115AsnSer: 1.115 ± 0.258
1.705AsnThr: 1.705 ± 0.374
2.098AsnVal: 2.098 ± 0.374
0.525AsnTrp: 0.525 ± 0.148
0.918AsnTyr: 0.918 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
6.557ProAla: 6.557 ± 0.998
0.459ProCys: 0.459 ± 0.187
3.475ProAsp: 3.475 ± 0.496
4.459ProGlu: 4.459 ± 0.534
1.115ProPhe: 1.115 ± 0.269
4.59ProGly: 4.59 ± 0.55
0.59ProHis: 0.59 ± 0.189
2.164ProIle: 2.164 ± 0.508
1.967ProLys: 1.967 ± 0.505
3.737ProLeu: 3.737 ± 0.48
0.656ProMet: 0.656 ± 0.208
1.115ProAsn: 1.115 ± 0.296
2.295ProPro: 2.295 ± 0.481
1.902ProGln: 1.902 ± 0.414
2.492ProArg: 2.492 ± 0.447
3.278ProSer: 3.278 ± 0.6
4.0ProThr: 4.0 ± 0.869
3.344ProVal: 3.344 ± 0.502
0.852ProTrp: 0.852 ± 0.259
1.049ProTyr: 1.049 ± 0.276
0.0ProXaa: 0.0 ± 0.0
Gln
3.737GlnAla: 3.737 ± 0.491
0.197GlnCys: 0.197 ± 0.11
1.377GlnAsp: 1.377 ± 0.262
2.229GlnGlu: 2.229 ± 0.401
1.311GlnPhe: 1.311 ± 0.217
2.426GlnGly: 2.426 ± 0.387
0.393GlnHis: 0.393 ± 0.19
1.639GlnIle: 1.639 ± 0.276
0.852GlnLys: 0.852 ± 0.287
2.492GlnLeu: 2.492 ± 0.605
1.049GlnMet: 1.049 ± 0.208
1.049GlnAsn: 1.049 ± 0.238
1.311GlnPro: 1.311 ± 0.376
0.918GlnGln: 0.918 ± 0.211
3.016GlnArg: 3.016 ± 0.489
1.311GlnSer: 1.311 ± 0.316
2.098GlnThr: 2.098 ± 0.345
3.41GlnVal: 3.41 ± 0.411
0.393GlnTrp: 0.393 ± 0.139
0.984GlnTyr: 0.984 ± 0.27
0.0GlnXaa: 0.0 ± 0.0
Arg
7.737ArgAla: 7.737 ± 0.862
0.852ArgCys: 0.852 ± 0.242
4.0ArgAsp: 4.0 ± 0.502
4.59ArgGlu: 4.59 ± 0.715
2.885ArgPhe: 2.885 ± 0.469
5.377ArgGly: 5.377 ± 0.601
1.443ArgHis: 1.443 ± 0.34
3.934ArgIle: 3.934 ± 0.636
2.951ArgLys: 2.951 ± 0.487
6.098ArgLeu: 6.098 ± 0.527
1.246ArgMet: 1.246 ± 0.253
1.77ArgAsn: 1.77 ± 0.451
2.819ArgPro: 2.819 ± 0.438
2.557ArgGln: 2.557 ± 0.431
6.95ArgArg: 6.95 ± 1.074
4.328ArgSer: 4.328 ± 0.578
4.065ArgThr: 4.065 ± 0.597
4.131ArgVal: 4.131 ± 0.623
0.721ArgTrp: 0.721 ± 0.234
2.229ArgTyr: 2.229 ± 0.407
0.0ArgXaa: 0.0 ± 0.0
Ser
7.082SerAla: 7.082 ± 0.785
0.197SerCys: 0.197 ± 0.107
3.737SerAsp: 3.737 ± 0.422
3.737SerGlu: 3.737 ± 0.512
1.77SerPhe: 1.77 ± 0.288
5.442SerGly: 5.442 ± 0.568
1.508SerHis: 1.508 ± 0.401
2.492SerIle: 2.492 ± 0.448
1.574SerLys: 1.574 ± 0.317
5.377SerLeu: 5.377 ± 0.667
1.115SerMet: 1.115 ± 0.224
1.967SerAsn: 1.967 ± 0.526
2.623SerPro: 2.623 ± 0.42
1.967SerGln: 1.967 ± 0.417
3.672SerArg: 3.672 ± 0.457
3.606SerSer: 3.606 ± 0.61
4.0SerThr: 4.0 ± 0.734
3.803SerVal: 3.803 ± 0.457
1.705SerTrp: 1.705 ± 0.343
1.639SerTyr: 1.639 ± 0.345
0.0SerXaa: 0.0 ± 0.0
Thr
6.164ThrAla: 6.164 ± 0.62
0.262ThrCys: 0.262 ± 0.151
4.131ThrAsp: 4.131 ± 0.535
5.18ThrGlu: 5.18 ± 0.765
2.426ThrPhe: 2.426 ± 0.505
5.573ThrGly: 5.573 ± 0.994
1.639ThrHis: 1.639 ± 0.272
3.082ThrIle: 3.082 ± 0.429
2.295ThrLys: 2.295 ± 0.486
5.508ThrLeu: 5.508 ± 0.707
0.984ThrMet: 0.984 ± 0.25
1.574ThrAsn: 1.574 ± 0.26
4.196ThrPro: 4.196 ± 0.56
1.443ThrGln: 1.443 ± 0.262
3.869ThrArg: 3.869 ± 0.551
3.672ThrSer: 3.672 ± 0.529
3.934ThrThr: 3.934 ± 0.505
4.918ThrVal: 4.918 ± 0.673
0.984ThrTrp: 0.984 ± 0.219
1.902ThrTyr: 1.902 ± 0.397
0.0ThrXaa: 0.0 ± 0.0
Val
8.131ValAla: 8.131 ± 0.706
0.918ValCys: 0.918 ± 0.316
4.721ValAsp: 4.721 ± 0.497
5.377ValGlu: 5.377 ± 0.622
2.098ValPhe: 2.098 ± 0.277
4.459ValGly: 4.459 ± 0.535
0.918ValHis: 0.918 ± 0.312
3.344ValIle: 3.344 ± 0.362
3.082ValLys: 3.082 ± 0.525
6.229ValLeu: 6.229 ± 0.67
1.246ValMet: 1.246 ± 0.322
2.229ValAsn: 2.229 ± 0.338
4.262ValPro: 4.262 ± 0.481
2.557ValGln: 2.557 ± 0.556
5.311ValArg: 5.311 ± 0.563
4.262ValSer: 4.262 ± 0.484
5.049ValThr: 5.049 ± 0.624
5.508ValVal: 5.508 ± 0.617
1.377ValTrp: 1.377 ± 0.284
1.377ValTyr: 1.377 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
2.229TrpAla: 2.229 ± 0.4
0.393TrpCys: 0.393 ± 0.172
1.443TrpAsp: 1.443 ± 0.26
1.246TrpGlu: 1.246 ± 0.284
0.525TrpPhe: 0.525 ± 0.157
1.77TrpGly: 1.77 ± 0.343
0.459TrpHis: 0.459 ± 0.201
0.459TrpIle: 0.459 ± 0.172
0.918TrpLys: 0.918 ± 0.209
1.311TrpLeu: 1.311 ± 0.347
0.328TrpMet: 0.328 ± 0.147
0.656TrpAsn: 0.656 ± 0.265
0.525TrpPro: 0.525 ± 0.259
0.525TrpGln: 0.525 ± 0.163
1.705TrpArg: 1.705 ± 0.423
1.311TrpSer: 1.311 ± 0.272
1.311TrpThr: 1.311 ± 0.276
1.115TrpVal: 1.115 ± 0.236
0.328TrpTrp: 0.328 ± 0.162
0.262TrpTyr: 0.262 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.492TyrAla: 2.492 ± 0.385
0.131TyrCys: 0.131 ± 0.091
2.295TyrAsp: 2.295 ± 0.495
2.033TyrGlu: 2.033 ± 0.449
0.721TyrPhe: 0.721 ± 0.201
3.147TyrGly: 3.147 ± 0.625
0.393TyrHis: 0.393 ± 0.183
0.721TyrIle: 0.721 ± 0.225
0.721TyrLys: 0.721 ± 0.182
2.098TyrLeu: 2.098 ± 0.372
0.984TyrMet: 0.984 ± 0.245
0.852TyrAsn: 0.852 ± 0.187
1.639TyrPro: 1.639 ± 0.349
0.984TyrGln: 0.984 ± 0.253
2.426TyrArg: 2.426 ± 0.382
1.508TyrSer: 1.508 ± 0.319
1.377TyrThr: 1.377 ± 0.249
1.77TyrVal: 1.77 ± 0.332
0.59TyrTrp: 0.59 ± 0.241
0.656TyrTyr: 0.656 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (15252 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski