Tobacco necrosis virus (strain A) (TNV-A)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphanecrovirus

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P22961|P6_TNVA Uncharacterized protein p6 OS=Tobacco necrosis virus (strain A) OX=12055 GN=ORF3 PE=4 SV=1
MM1 pKa = 8.43DD2 pKa = 3.9YY3 pKa = 9.48QTDD6 pKa = 3.58VTEE9 pKa = 5.35DD10 pKa = 3.08NVQIRR15 pKa = 11.84GRR17 pKa = 11.84ARR19 pKa = 11.84SVEE22 pKa = 4.17GKK24 pKa = 9.35KK25 pKa = 10.22HH26 pKa = 5.76NGSGLTGVKK35 pKa = 9.46RR36 pKa = 11.84HH37 pKa = 5.83AVSEE41 pKa = 4.38TSQKK45 pKa = 9.99SQQGTGNGTMTNIAEE60 pKa = 4.27EE61 pKa = 3.98QTITVTYY68 pKa = 10.55NFNFF72 pKa = 3.71

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P22962|P7_TNVA Uncharacterized protein p7 OS=Tobacco necrosis virus (strain A) OX=12055 GN=ORF5 PE=4 SV=1
MM1 pKa = 7.54PKK3 pKa = 9.78VEE5 pKa = 4.03SHH7 pKa = 4.61HH8 pKa = 4.57WRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ILGNRR18 pKa = 11.84LDD20 pKa = 3.99GRR22 pKa = 11.84GVVPPTTHH30 pKa = 6.34HH31 pKa = 7.01SGYY34 pKa = 9.78QWYY37 pKa = 7.06TTMAGSAKK45 pKa = 10.03VLCTKK50 pKa = 10.58NPWKK54 pKa = 10.56RR55 pKa = 11.84GGGGG59 pKa = 3.02

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1187

56

724

237.4

26.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.498 ± 1.842

1.853 ± 0.442

4.128 ± 0.527

4.718 ± 1.31

3.875 ± 0.815

7.751 ± 1.002

2.696 ± 0.974

5.223 ± 1.254

5.981 ± 0.928

8.088 ± 1.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.187

5.392 ± 0.761

5.055 ± 0.86

5.139 ± 0.633

6.318 ± 0.754

6.066 ± 0.555

6.234 ± 1.353

6.571 ± 0.713

1.264 ± 0.466

3.875 ± 0.499
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski