Streptococcus virus 7201
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9MCK4|Q9MCK4_9CAUD MPL-7201 OS=Streptococcus virus 7201 OX=112023 GN=mpl-7201 PE=4 SV=1
MM1 pKa = 7.31 SVSKK5 pKa = 9.71 TSIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.69 IEE28 pKa = 4.01 SAKK31 pKa = 10.51 QYY33 pKa = 10.21 IINAVGSDD41 pKa = 3.28 SKK43 pKa = 11.27 FYY45 pKa = 10.83 DD46 pKa = 3.78 LEE48 pKa = 4.23 NVQPLFDD55 pKa = 3.7 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.49 TVTYY78 pKa = 9.35 PINLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.4 ATYY98 pKa = 10.89 SEE100 pKa = 4.8 VVANGG105 pKa = 3.05
Molecular weight: 11.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.91
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.783
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.113
Patrickios 0.477
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.991
Protein with the highest isoelectric point:
>tr|Q9MCK3|Q9MCK3_9CAUD ORF27 OS=Streptococcus virus 7201 OX=112023 GN=orf27 PE=4 SV=1
MM1 pKa = 7.36 ARR3 pKa = 11.84 VRR5 pKa = 11.84 YY6 pKa = 9.3 LPSDD10 pKa = 3.51 FRR12 pKa = 11.84 FKK14 pKa = 11.35 ADD16 pKa = 3.23 FGTYY20 pKa = 8.85 QSSPNKK26 pKa = 8.44 FTGVSVPKK34 pKa = 10.14 FVKK37 pKa = 10.38 QFTLHH42 pKa = 5.9 YY43 pKa = 9.16 KK44 pKa = 9.46 PHH46 pKa = 6.1 TRR48 pKa = 11.84 TLNQEE53 pKa = 3.69 YY54 pKa = 10.23 LAIQNGEE61 pKa = 3.55 NDD63 pKa = 3.11 TRR65 pKa = 11.84 VIVIRR70 pKa = 11.84 HH71 pKa = 4.91 NSKK74 pKa = 9.81 VLEE77 pKa = 4.45 GQVVTLNGTQYY88 pKa = 11.15 DD89 pKa = 3.86 IVRR92 pKa = 11.84 ISPDD96 pKa = 3.02 EE97 pKa = 3.9 NFGFNHH103 pKa = 6.67 YY104 pKa = 11.11 DD105 pKa = 3.11 FLTLKK110 pKa = 10.2 KK111 pKa = 9.72 RR112 pKa = 11.84 KK113 pKa = 9.51 KK114 pKa = 10.37 VGG116 pKa = 3.08
Molecular weight: 13.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.119
IPC2_protein 9.399
IPC_protein 9.428
Toseland 10.014
ProMoST 9.706
Dawson 10.218
Bjellqvist 9.882
Wikipedia 10.394
Rodwell 10.657
Grimsley 10.306
Solomon 10.248
Lehninger 10.218
Nozaki 9.97
DTASelect 9.882
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.131
Patrickios 10.277
IPC_peptide 10.248
IPC2_peptide 8.404
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
10960
47
1554
243.6
27.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.761 ± 0.473
0.566 ± 0.124
6.277 ± 0.239
6.359 ± 0.596
4.234 ± 0.264
6.715 ± 0.545
1.469 ± 0.116
6.542 ± 0.264
8.102 ± 0.551
7.892 ± 0.379
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.345 ± 0.179
6.442 ± 0.299
3.002 ± 0.166
4.644 ± 0.253
3.768 ± 0.29
6.77 ± 0.346
6.515 ± 0.471
6.031 ± 0.201
1.533 ± 0.176
4.033 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here