Prevotella nigrescens ATCC 33563

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella nigrescens

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9DAP5|F9DAP5_9BACT DNA primase OS=Prevotella nigrescens ATCC 33563 OX=997352 GN=traP PE=4 SV=1
MM1 pKa = 6.77EE2 pKa = 4.49TKK4 pKa = 9.81EE5 pKa = 4.04LNEE8 pKa = 3.36ARR10 pKa = 11.84IYY12 pKa = 10.47VGTYY16 pKa = 9.66AKK18 pKa = 10.48YY19 pKa = 11.08NNGSLQGEE27 pKa = 4.35WVEE30 pKa = 5.59LSDD33 pKa = 6.78FYY35 pKa = 11.83DD36 pKa = 4.18LDD38 pKa = 4.99DD39 pKa = 5.71FIEE42 pKa = 4.58HH43 pKa = 6.76CAEE46 pKa = 3.43IHH48 pKa = 6.13EE49 pKa = 5.0DD50 pKa = 3.74EE51 pKa = 5.0EE52 pKa = 4.7EE53 pKa = 4.62PEE55 pKa = 4.68LMFQAWEE62 pKa = 4.39EE63 pKa = 4.26IPDD66 pKa = 3.75GLIDD70 pKa = 4.19EE71 pKa = 4.75GHH73 pKa = 6.58LEE75 pKa = 4.11EE76 pKa = 5.78NFFEE80 pKa = 5.82LRR82 pKa = 11.84DD83 pKa = 3.77EE84 pKa = 5.04LDD86 pKa = 3.4RR87 pKa = 11.84LSDD90 pKa = 3.81KK91 pKa = 10.61EE92 pKa = 4.99QEE94 pKa = 4.66TFWVWADD101 pKa = 3.27GNNAQFTQDD110 pKa = 3.13AYY112 pKa = 11.56SLVKK116 pKa = 10.45AFQSAYY122 pKa = 8.28IGSYY126 pKa = 10.59ASRR129 pKa = 11.84EE130 pKa = 4.07DD131 pKa = 3.69FAEE134 pKa = 4.24EE135 pKa = 3.76LAKK138 pKa = 10.16MEE140 pKa = 4.7NDD142 pKa = 3.78LPDD145 pKa = 3.5FALIYY150 pKa = 10.6FDD152 pKa = 3.84FSKK155 pKa = 11.21YY156 pKa = 10.79ADD158 pKa = 4.53DD159 pKa = 5.77LFCTDD164 pKa = 3.63FWYY167 pKa = 10.77KK168 pKa = 10.62DD169 pKa = 3.26GYY171 pKa = 9.62VFRR174 pKa = 11.84NNN176 pKa = 3.2

Molecular weight: 20.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9DD20|F9DD20_9BACT NADH-quinone oxidoreductase subunit H OS=Prevotella nigrescens ATCC 33563 OX=997352 GN=nuoH PE=3 SV=1
MM1 pKa = 7.67LYY3 pKa = 10.11SACKK7 pKa = 9.48DD8 pKa = 3.49TEE10 pKa = 4.33KK11 pKa = 10.95KK12 pKa = 10.7LKK14 pKa = 10.52LQATTNKK21 pKa = 8.91IAVVAGLIFQPDD33 pKa = 4.29ALCLRR38 pKa = 11.84CGMAGKK44 pKa = 10.16RR45 pKa = 11.84KK46 pKa = 8.77PYY48 pKa = 9.78KK49 pKa = 10.11GRR51 pKa = 11.84HH52 pKa = 4.55TVTGGCPRR60 pKa = 11.84CQTTPLRR67 pKa = 11.84SLLWGRR73 pKa = 11.84TLQKK77 pKa = 10.97SLFYY81 pKa = 10.7GSKK84 pKa = 9.9QALLQCKK91 pKa = 8.66TMGFAAPRR99 pKa = 11.84RR100 pKa = 11.84AFRR103 pKa = 11.84IFLTMRR109 pKa = 11.84WLHH112 pKa = 5.48NLEE115 pKa = 4.27IYY117 pKa = 9.64GICLRR122 pKa = 11.84IFHH125 pKa = 6.3VAAISPLACYY135 pKa = 7.85FTFITLLPAIFYY147 pKa = 10.63LSVAILAAIHH157 pKa = 5.65SRR159 pKa = 11.84RR160 pKa = 11.84SSLRR164 pKa = 11.84FRR166 pKa = 11.84VNDD169 pKa = 3.39SPIRR173 pKa = 11.84LTMKK177 pKa = 10.45QNTPKK182 pKa = 10.58FF183 pKa = 3.72

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2420

0

2420

758684

39

2536

313.5

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.192 ± 0.044

1.283 ± 0.018

5.433 ± 0.034

6.233 ± 0.057

4.621 ± 0.039

6.537 ± 0.053

1.975 ± 0.023

7.096 ± 0.049

7.337 ± 0.049

8.905 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.028

5.517 ± 0.045

3.475 ± 0.025

3.581 ± 0.029

4.579 ± 0.039

5.822 ± 0.038

5.868 ± 0.04

6.379 ± 0.035

1.1 ± 0.019

4.292 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski