Pelagicola litoralis
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3367 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U7MRW7|A0A4U7MRW7_9RHOB Uncharacterized protein OS=Pelagicola litoralis OX=420403 GN=FAP39_17065 PE=4 SV=1
GG1 pKa = 6.82 ATQQVTVPVTVTDD14 pKa = 3.6 STGATDD20 pKa = 3.63 TEE22 pKa = 4.24 NLVITVTGTNDD33 pKa = 2.94 GPAVSGPVTLPGGTEE48 pKa = 3.91 DD49 pKa = 3.44 TSVQITAAQLLEE61 pKa = 4.4 HH62 pKa = 6.59 ATDD65 pKa = 4.66 ADD67 pKa = 3.76 TGDD70 pKa = 3.55 QLAVTGLTASHH81 pKa = 5.91 GTITGDD87 pKa = 3.08 AATGFTFTPDD97 pKa = 2.89 ANYY100 pKa = 10.54 NGPVSLSYY108 pKa = 10.47 TVTDD112 pKa = 3.3 GHH114 pKa = 6.54 GGSVAQTASLTLGAVGDD131 pKa = 3.79 PAIIGGVDD139 pKa = 3.05 TGDD142 pKa = 3.37 VTEE145 pKa = 4.84 NKK147 pKa = 9.95 AGQDD151 pKa = 3.45 MSPDD155 pKa = 3.41 YY156 pKa = 10.13 AQPGMATLGRR166 pKa = 11.84 AQLYY170 pKa = 10.83 ADD172 pKa = 4.11 GQLTILDD179 pKa = 4.57 PDD181 pKa = 3.53 TGEE184 pKa = 4.05 NQFDD188 pKa = 3.9 TRR190 pKa = 11.84 GSMGMNYY197 pKa = 10.11 SGTYY201 pKa = 10.01 GDD203 pKa = 5.35 LRR205 pKa = 11.84 LNSDD209 pKa = 4.2 GSWHH213 pKa = 6.26 YY214 pKa = 11.19 YY215 pKa = 9.58 GDD217 pKa = 3.92 AGHH220 pKa = 6.8 IRR222 pKa = 11.84 VTGGRR227 pKa = 11.84 TTTRR231 pKa = 11.84 GTAIDD236 pKa = 3.64 QLGEE240 pKa = 4.22 GQTLTDD246 pKa = 4.46 TITVYY251 pKa = 11.07 SKK253 pKa = 11.02 DD254 pKa = 3.4 GTAHH258 pKa = 7.58 DD259 pKa = 3.75 ITITIHH265 pKa = 6.11 GSNDD269 pKa = 2.92 RR270 pKa = 11.84 PYY272 pKa = 10.65 CSSEE276 pKa = 3.92 VALTPGTEE284 pKa = 4.22 DD285 pKa = 3.16 TAQTLTKK292 pKa = 10.45 AQLLANTVEE301 pKa = 4.01 VDD303 pKa = 3.29 ANDD306 pKa = 3.71 AGKK309 pKa = 10.77 LSIAGLRR316 pKa = 11.84 ADD318 pKa = 4.1 HH319 pKa = 7.17 GSIRR323 pKa = 11.84 DD324 pKa = 3.92 NGDD327 pKa = 2.64 GTYY330 pKa = 10.28 TFTPARR336 pKa = 11.84 DD337 pKa = 3.62 YY338 pKa = 11.55 NGAVHH343 pKa = 6.2 FTYY346 pKa = 10.23 DD347 pKa = 3.07 VKK349 pKa = 10.92 DD350 pKa = 3.26 AHH352 pKa = 6.92 GGVTHH357 pKa = 7.18 TGATTTLAAVSDD369 pKa = 3.81 AAIIGGTDD377 pKa = 2.98 TGSATEE383 pKa = 3.87 DD384 pKa = 3.46 HH385 pKa = 5.47 VTGRR389 pKa = 11.84 IVISGLLTVSDD400 pKa = 4.52 PDD402 pKa = 4.13 GPSQEE407 pKa = 4.2 HH408 pKa = 5.16 FQYY411 pKa = 11.05 SQFGEE416 pKa = 4.24 HH417 pKa = 7.14 AISDD421 pKa = 3.95 PFGGNLHH428 pKa = 6.76 IDD430 pKa = 3.73 SAGSWSYY437 pKa = 9.36 VTDD440 pKa = 3.5 NSNPAVQQLAAGQEE454 pKa = 4.0 GHH456 pKa = 5.06 ATYY459 pKa = 10.36 RR460 pKa = 11.84 VRR462 pKa = 11.84 SSDD465 pKa = 3.16 GTTHH469 pKa = 6.83 QIQITIHH476 pKa = 5.47 GTNDD480 pKa = 3.23 APVLSASTASATEE493 pKa = 4.6 DD494 pKa = 3.72 GQSVTGQVSGTDD506 pKa = 3.2 VDD508 pKa = 3.74 TGDD511 pKa = 3.66 ALAYY515 pKa = 10.19 SLGSAAPAGFTLNPDD530 pKa = 3.81 GSWSFDD536 pKa = 3.69 PADD539 pKa = 3.58 AAYY542 pKa = 10.19 QHH544 pKa = 6.84 LAAGATQQVTVPVTVTDD561 pKa = 3.6 STGATDD567 pKa = 3.59 TQNLVITVTGTNDD580 pKa = 3.06 GPTVSGPVTLPGGTEE595 pKa = 3.91 DD596 pKa = 3.44 TSVQITAAQLLAHH609 pKa = 6.49 ATDD612 pKa = 4.02 IDD614 pKa = 3.89 TGDD617 pKa = 3.21 TT618 pKa = 3.49
Molecular weight: 62.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.923
IPC_protein 3.973
Toseland 3.745
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.948
Rodwell 3.795
Grimsley 3.643
Solomon 3.986
Lehninger 3.948
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.795
EMBOSS 3.961
Sillero 4.113
Patrickios 1.952
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.98
Protein with the highest isoelectric point:
>tr|A0A4U7N8S8|A0A4U7N8S8_9RHOB ABC transporter ATP-binding protein OS=Pelagicola litoralis OX=420403 GN=FAP39_00465 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.34 SLSAA44 pKa = 3.83
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3367
0
3367
1051415
8
2823
312.3
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.347 ± 0.052
0.931 ± 0.013
6.286 ± 0.042
5.626 ± 0.04
3.852 ± 0.023
8.323 ± 0.041
2.208 ± 0.02
5.403 ± 0.029
3.714 ± 0.033
9.757 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.941 ± 0.022
2.883 ± 0.023
4.799 ± 0.026
3.565 ± 0.024
6.043 ± 0.038
5.599 ± 0.028
5.657 ± 0.028
7.339 ± 0.033
1.389 ± 0.017
2.338 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here