Aeromonas phage D6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TVZ8|A0A514TVZ8_9CAUD Uncharacterized protein OS=Aeromonas phage D6 OX=2593322 GN=D6_0038 PE=4 SV=1
MM1 pKa = 7.61CGSDD5 pKa = 3.66TPFVLQDD12 pKa = 3.84TIVHH16 pKa = 6.83LDD18 pKa = 3.65LLIDD22 pKa = 3.74EE23 pKa = 4.81VDD25 pKa = 3.5RR26 pKa = 11.84SKK28 pKa = 11.67DD29 pKa = 3.13LANFYY34 pKa = 11.14FEE36 pKa = 4.86GNIFIIVEE44 pKa = 4.12DD45 pKa = 4.26PYY47 pKa = 11.4GTVV50 pKa = 2.95

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TWL3|A0A514TWL3_9CAUD Uncharacterized protein OS=Aeromonas phage D6 OX=2593322 GN=D6_0257 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.83DD4 pKa = 3.99NLPRR8 pKa = 11.84YY9 pKa = 9.56IPLDD13 pKa = 3.74YY14 pKa = 10.25IGKK17 pKa = 8.89IRR19 pKa = 11.84SRR21 pKa = 11.84VKK23 pKa = 10.14KK24 pKa = 9.99HH25 pKa = 5.83DD26 pKa = 3.67AARR29 pKa = 11.84LKK31 pKa = 11.12AMYY34 pKa = 9.56EE35 pKa = 3.8RR36 pKa = 11.84ARR38 pKa = 11.84NVVKK42 pKa = 11.03NNTQSKK48 pKa = 9.89

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

262

0

262

79372

30

2794

302.9

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.487 ± 0.185

0.944 ± 0.053

6.384 ± 0.091

6.738 ± 0.137

4.566 ± 0.135

6.829 ± 0.137

2.021 ± 0.077

6.26 ± 0.116

6.249 ± 0.114

8.281 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.182 ± 0.093

5.381 ± 0.128

4.018 ± 0.113

3.281 ± 0.101

4.873 ± 0.123

6.037 ± 0.1

6.283 ± 0.19

7.15 ± 0.144

1.179 ± 0.063

3.857 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski