Amino acid dipepetide frequency for Pelagibacter phage HTVC025P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.171AlaAla: 0.171 ± 0.191
0.171AlaCys: 0.171 ± 0.118
4.775AlaAsp: 4.775 ± 0.533
3.752AlaGlu: 3.752 ± 0.726
2.047AlaPhe: 2.047 ± 0.428
5.457AlaGly: 5.457 ± 1.131
0.512AlaHis: 0.512 ± 0.189
4.861AlaIle: 4.861 ± 0.684
5.202AlaLys: 5.202 ± 0.806
5.031AlaLeu: 5.031 ± 0.865
1.876AlaMet: 1.876 ± 0.352
6.651AlaAsn: 6.651 ± 2.731
2.217AlaPro: 2.217 ± 0.395
2.899AlaGln: 2.899 ± 0.44
2.814AlaArg: 2.814 ± 0.557
6.225AlaSer: 6.225 ± 1.036
6.31AlaThr: 6.31 ± 1.222
4.264AlaVal: 4.264 ± 0.725
0.597AlaTrp: 0.597 ± 0.238
2.729AlaTyr: 2.729 ± 0.553
0.0AlaXaa: 0.0 ± 0.0
Cys
0.426CysAla: 0.426 ± 0.22
0.085CysCys: 0.085 ± 0.106
0.085CysAsp: 0.085 ± 0.083
0.512CysGlu: 0.512 ± 0.232
0.853CysPhe: 0.853 ± 0.318
0.767CysGly: 0.767 ± 0.278
0.171CysHis: 0.171 ± 0.142
1.194CysIle: 1.194 ± 0.312
0.512CysLys: 0.512 ± 0.212
1.023CysLeu: 1.023 ± 0.34
0.085CysMet: 0.085 ± 0.084
0.512CysAsn: 0.512 ± 0.275
0.341CysPro: 0.341 ± 0.178
0.256CysGln: 0.256 ± 0.136
0.512CysArg: 0.512 ± 0.179
0.682CysSer: 0.682 ± 0.248
0.341CysThr: 0.341 ± 0.174
0.597CysVal: 0.597 ± 0.232
0.0CysTrp: 0.0 ± 0.0
0.341CysTyr: 0.341 ± 0.222
0.0CysXaa: 0.0 ± 0.0
Asp
4.349AspAla: 4.349 ± 0.751
0.426AspCys: 0.426 ± 0.279
2.985AspAsp: 2.985 ± 0.409
4.093AspGlu: 4.093 ± 0.692
3.923AspPhe: 3.923 ± 0.689
3.923AspGly: 3.923 ± 0.751
0.512AspHis: 0.512 ± 0.242
4.178AspIle: 4.178 ± 0.887
4.093AspLys: 4.093 ± 0.654
4.093AspLeu: 4.093 ± 0.566
1.279AspMet: 1.279 ± 0.303
2.899AspAsn: 2.899 ± 0.552
1.876AspPro: 1.876 ± 0.405
1.279AspGln: 1.279 ± 0.358
2.217AspArg: 2.217 ± 0.403
2.729AspSer: 2.729 ± 0.522
4.349AspThr: 4.349 ± 0.612
3.24AspVal: 3.24 ± 0.628
0.767AspTrp: 0.767 ± 0.313
2.643AspTyr: 2.643 ± 0.59
0.0AspXaa: 0.0 ± 0.0
Glu
5.628GluAla: 5.628 ± 0.886
0.512GluCys: 0.512 ± 0.193
2.729GluAsp: 2.729 ± 0.507
5.202GluGlu: 5.202 ± 0.696
3.326GluPhe: 3.326 ± 0.579
3.155GluGly: 3.155 ± 0.704
0.597GluHis: 0.597 ± 0.253
5.287GluIle: 5.287 ± 0.668
5.031GluLys: 5.031 ± 0.88
5.372GluLeu: 5.372 ± 0.735
1.535GluMet: 1.535 ± 0.28
4.434GluAsn: 4.434 ± 0.835
1.364GluPro: 1.364 ± 0.356
3.667GluGln: 3.667 ± 0.541
2.643GluArg: 2.643 ± 0.378
2.985GluSer: 2.985 ± 0.434
4.093GluThr: 4.093 ± 0.409
3.155GluVal: 3.155 ± 0.523
0.597GluTrp: 0.597 ± 0.245
2.132GluTyr: 2.132 ± 0.428
0.0GluXaa: 0.0 ± 0.0
Phe
2.558PheAla: 2.558 ± 0.448
0.597PheCys: 0.597 ± 0.339
3.24PheAsp: 3.24 ± 0.498
2.558PheGlu: 2.558 ± 0.355
1.535PhePhe: 1.535 ± 0.401
3.24PheGly: 3.24 ± 0.562
0.426PheHis: 0.426 ± 0.21
3.496PheIle: 3.496 ± 0.812
4.264PheLys: 4.264 ± 0.849
3.07PheLeu: 3.07 ± 0.504
0.682PheMet: 0.682 ± 0.244
3.667PheAsn: 3.667 ± 0.613
1.705PhePro: 1.705 ± 0.371
1.023PheGln: 1.023 ± 0.241
1.45PheArg: 1.45 ± 0.357
3.411PheSer: 3.411 ± 0.737
1.961PheThr: 1.961 ± 0.429
2.047PheVal: 2.047 ± 0.368
0.085PheTrp: 0.085 ± 0.094
1.194PheTyr: 1.194 ± 0.307
0.0PheXaa: 0.0 ± 0.0
Gly
4.093GlyAla: 4.093 ± 0.666
0.341GlyCys: 0.341 ± 0.165
4.178GlyAsp: 4.178 ± 0.671
3.411GlyGlu: 3.411 ± 0.664
2.985GlyPhe: 2.985 ± 0.609
7.589GlyGly: 7.589 ± 3.355
0.853GlyHis: 0.853 ± 0.304
3.496GlyIle: 3.496 ± 0.503
5.031GlyLys: 5.031 ± 0.805
4.519GlyLeu: 4.519 ± 0.611
1.279GlyMet: 1.279 ± 0.355
4.093GlyAsn: 4.093 ± 0.522
0.0GlyPro: 0.0 ± 0.0
3.155GlyGln: 3.155 ± 0.612
2.899GlyArg: 2.899 ± 0.52
6.395GlySer: 6.395 ± 1.73
6.14GlyThr: 6.14 ± 1.519
3.923GlyVal: 3.923 ± 0.421
0.682GlyTrp: 0.682 ± 0.248
2.985GlyTyr: 2.985 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
0.512HisAla: 0.512 ± 0.189
0.597HisCys: 0.597 ± 0.268
0.426HisAsp: 0.426 ± 0.174
0.426HisGlu: 0.426 ± 0.173
0.682HisPhe: 0.682 ± 0.311
0.512HisGly: 0.512 ± 0.226
0.512HisHis: 0.512 ± 0.241
0.597HisIle: 0.597 ± 0.279
0.938HisLys: 0.938 ± 0.316
1.62HisLeu: 1.62 ± 0.449
0.171HisMet: 0.171 ± 0.131
0.597HisAsn: 0.597 ± 0.24
0.171HisPro: 0.171 ± 0.125
0.682HisGln: 0.682 ± 0.297
0.767HisArg: 0.767 ± 0.297
0.767HisSer: 0.767 ± 0.366
1.279HisThr: 1.279 ± 0.303
0.256HisVal: 0.256 ± 0.135
0.085HisTrp: 0.085 ± 0.085
0.682HisTyr: 0.682 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
6.054IleAla: 6.054 ± 1.346
0.853IleCys: 0.853 ± 0.281
4.093IleAsp: 4.093 ± 0.699
4.264IleGlu: 4.264 ± 0.574
2.047IlePhe: 2.047 ± 0.45
4.093IleGly: 4.093 ± 0.7
0.767IleHis: 0.767 ± 0.304
4.861IleIle: 4.861 ± 0.951
5.628IleLys: 5.628 ± 0.94
5.628IleLeu: 5.628 ± 0.824
1.194IleMet: 1.194 ± 0.382
5.116IleAsn: 5.116 ± 0.695
1.961IlePro: 1.961 ± 0.459
2.473IleGln: 2.473 ± 0.557
2.899IleArg: 2.899 ± 0.549
5.031IleSer: 5.031 ± 0.847
5.116IleThr: 5.116 ± 1.231
4.605IleVal: 4.605 ± 0.552
0.682IleTrp: 0.682 ± 0.218
2.132IleTyr: 2.132 ± 0.537
0.0IleXaa: 0.0 ± 0.0
Lys
5.031LysAla: 5.031 ± 0.741
0.682LysCys: 0.682 ± 0.271
4.434LysAsp: 4.434 ± 0.899
5.457LysGlu: 5.457 ± 1.004
3.837LysPhe: 3.837 ± 0.862
3.837LysGly: 3.837 ± 0.672
1.364LysHis: 1.364 ± 0.444
5.457LysIle: 5.457 ± 0.789
7.419LysLys: 7.419 ± 1.278
8.613LysLeu: 8.613 ± 1.087
2.047LysMet: 2.047 ± 0.519
5.202LysAsn: 5.202 ± 0.833
2.473LysPro: 2.473 ± 0.669
4.605LysGln: 4.605 ± 0.751
2.814LysArg: 2.814 ± 0.612
4.946LysSer: 4.946 ± 0.708
4.519LysThr: 4.519 ± 0.719
5.457LysVal: 5.457 ± 0.568
0.853LysTrp: 0.853 ± 0.219
3.326LysTyr: 3.326 ± 0.664
0.0LysXaa: 0.0 ± 0.0
Leu
6.054LeuAla: 6.054 ± 0.615
0.682LeuCys: 0.682 ± 0.306
5.031LeuAsp: 5.031 ± 0.627
6.395LeuGlu: 6.395 ± 0.816
2.302LeuPhe: 2.302 ± 0.382
4.946LeuGly: 4.946 ± 0.677
1.109LeuHis: 1.109 ± 0.362
5.287LeuIle: 5.287 ± 0.984
5.884LeuLys: 5.884 ± 0.721
5.628LeuLeu: 5.628 ± 0.798
1.791LeuMet: 1.791 ± 0.379
6.395LeuAsn: 6.395 ± 0.591
3.752LeuPro: 3.752 ± 0.549
3.326LeuGln: 3.326 ± 0.67
4.519LeuArg: 4.519 ± 0.676
4.946LeuSer: 4.946 ± 0.794
4.434LeuThr: 4.434 ± 0.581
5.202LeuVal: 5.202 ± 0.47
1.109LeuTrp: 1.109 ± 0.341
1.791LeuTyr: 1.791 ± 0.302
0.0LeuXaa: 0.0 ± 0.0
Met
1.364MetAla: 1.364 ± 0.281
0.512MetCys: 0.512 ± 0.214
0.512MetAsp: 0.512 ± 0.235
0.682MetGlu: 0.682 ± 0.252
0.853MetPhe: 0.853 ± 0.302
1.109MetGly: 1.109 ± 0.296
0.341MetHis: 0.341 ± 0.164
1.364MetIle: 1.364 ± 0.436
1.961MetLys: 1.961 ± 0.386
1.961MetLeu: 1.961 ± 0.428
0.767MetMet: 0.767 ± 0.286
1.364MetAsn: 1.364 ± 0.35
0.597MetPro: 0.597 ± 0.243
0.767MetGln: 0.767 ± 0.249
0.682MetArg: 0.682 ± 0.186
1.535MetSer: 1.535 ± 0.429
1.62MetThr: 1.62 ± 0.389
0.767MetVal: 0.767 ± 0.329
0.0MetTrp: 0.0 ± 0.0
0.426MetTyr: 0.426 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
4.605AsnAla: 4.605 ± 1.022
0.341AsnCys: 0.341 ± 0.203
3.752AsnAsp: 3.752 ± 0.714
4.264AsnGlu: 4.264 ± 0.652
2.558AsnPhe: 2.558 ± 0.418
5.202AsnGly: 5.202 ± 0.889
0.682AsnHis: 0.682 ± 0.216
4.946AsnIle: 4.946 ± 1.168
5.287AsnLys: 5.287 ± 0.659
4.775AsnLeu: 4.775 ± 0.516
1.109AsnMet: 1.109 ± 0.316
3.155AsnAsn: 3.155 ± 0.537
2.388AsnPro: 2.388 ± 0.364
2.302AsnGln: 2.302 ± 0.377
2.985AsnArg: 2.985 ± 0.442
5.116AsnSer: 5.116 ± 0.758
4.434AsnThr: 4.434 ± 1.166
4.775AsnVal: 4.775 ± 1.328
0.767AsnTrp: 0.767 ± 0.234
3.496AsnTyr: 3.496 ± 0.601
0.0AsnXaa: 0.0 ± 0.0
Pro
1.62ProAla: 1.62 ± 0.371
0.256ProCys: 0.256 ± 0.187
1.535ProAsp: 1.535 ± 0.316
3.24ProGlu: 3.24 ± 0.499
1.876ProPhe: 1.876 ± 0.526
0.0ProGly: 0.0 ± 0.0
0.426ProHis: 0.426 ± 0.213
1.961ProIle: 1.961 ± 0.509
2.473ProLys: 2.473 ± 0.479
1.705ProLeu: 1.705 ± 0.417
0.597ProMet: 0.597 ± 0.243
1.705ProAsn: 1.705 ± 0.295
1.279ProPro: 1.279 ± 0.42
1.279ProGln: 1.279 ± 0.387
0.682ProArg: 0.682 ± 0.224
2.643ProSer: 2.643 ± 0.501
2.302ProThr: 2.302 ± 0.596
2.473ProVal: 2.473 ± 0.433
0.085ProTrp: 0.085 ± 0.078
1.791ProTyr: 1.791 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
3.496GlnAla: 3.496 ± 0.506
0.341GlnCys: 0.341 ± 0.158
1.876GlnAsp: 1.876 ± 0.329
2.899GlnGlu: 2.899 ± 0.622
1.961GlnPhe: 1.961 ± 0.367
2.729GlnGly: 2.729 ± 0.49
0.597GlnHis: 0.597 ± 0.282
2.558GlnIle: 2.558 ± 0.38
3.837GlnLys: 3.837 ± 0.756
3.326GlnLeu: 3.326 ± 0.711
0.767GlnMet: 0.767 ± 0.245
1.705GlnAsn: 1.705 ± 0.37
1.023GlnPro: 1.023 ± 0.272
2.643GlnGln: 2.643 ± 0.919
1.62GlnArg: 1.62 ± 0.396
3.837GlnSer: 3.837 ± 0.597
2.729GlnThr: 2.729 ± 0.545
2.132GlnVal: 2.132 ± 0.451
0.512GlnTrp: 0.512 ± 0.229
1.876GlnTyr: 1.876 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
1.876ArgAla: 1.876 ± 0.542
0.426ArgCys: 0.426 ± 0.2
2.558ArgAsp: 2.558 ± 0.352
2.388ArgGlu: 2.388 ± 0.456
2.047ArgPhe: 2.047 ± 0.448
2.473ArgGly: 2.473 ± 0.496
0.682ArgHis: 0.682 ± 0.319
2.558ArgIle: 2.558 ± 0.43
4.178ArgLys: 4.178 ± 0.722
4.434ArgLeu: 4.434 ± 0.64
0.171ArgMet: 0.171 ± 0.117
3.155ArgAsn: 3.155 ± 0.537
1.194ArgPro: 1.194 ± 0.318
1.535ArgGln: 1.535 ± 0.403
1.705ArgArg: 1.705 ± 0.36
2.047ArgSer: 2.047 ± 0.362
2.132ArgThr: 2.132 ± 0.509
2.132ArgVal: 2.132 ± 0.566
0.256ArgTrp: 0.256 ± 0.157
2.388ArgTyr: 2.388 ± 0.512
0.0ArgXaa: 0.0 ± 0.0
Ser
5.884SerAla: 5.884 ± 1.153
0.426SerCys: 0.426 ± 0.209
4.434SerAsp: 4.434 ± 0.589
3.581SerGlu: 3.581 ± 0.485
3.326SerPhe: 3.326 ± 0.599
6.651SerGly: 6.651 ± 1.894
0.767SerHis: 0.767 ± 0.343
5.116SerIle: 5.116 ± 0.82
5.457SerLys: 5.457 ± 0.684
5.287SerLeu: 5.287 ± 0.641
0.767SerMet: 0.767 ± 0.395
3.837SerAsn: 3.837 ± 0.682
1.961SerPro: 1.961 ± 0.418
2.814SerGln: 2.814 ± 0.744
3.411SerArg: 3.411 ± 0.496
6.31SerSer: 6.31 ± 1.264
4.264SerThr: 4.264 ± 0.704
4.434SerVal: 4.434 ± 0.69
0.938SerTrp: 0.938 ± 0.361
2.047SerTyr: 2.047 ± 0.5
0.0SerXaa: 0.0 ± 0.0
Thr
6.054ThrAla: 6.054 ± 1.205
0.767ThrCys: 0.767 ± 0.342
3.496ThrAsp: 3.496 ± 0.971
4.008ThrGlu: 4.008 ± 0.559
2.388ThrPhe: 2.388 ± 0.5
4.946ThrGly: 4.946 ± 0.949
0.682ThrHis: 0.682 ± 0.252
4.861ThrIle: 4.861 ± 0.985
4.775ThrLys: 4.775 ± 0.643
4.775ThrLeu: 4.775 ± 0.514
1.023ThrMet: 1.023 ± 0.268
4.775ThrAsn: 4.775 ± 0.832
2.388ThrPro: 2.388 ± 0.458
2.729ThrGln: 2.729 ± 0.369
1.705ThrArg: 1.705 ± 0.472
4.008ThrSer: 4.008 ± 0.844
6.14ThrThr: 6.14 ± 1.464
5.628ThrVal: 5.628 ± 1.838
0.597ThrTrp: 0.597 ± 0.288
3.07ThrTyr: 3.07 ± 0.523
0.0ThrXaa: 0.0 ± 0.0
Val
6.054ValAla: 6.054 ± 1.669
0.597ValCys: 0.597 ± 0.245
3.155ValAsp: 3.155 ± 0.487
3.24ValGlu: 3.24 ± 0.516
2.302ValPhe: 2.302 ± 0.359
4.178ValGly: 4.178 ± 0.49
0.512ValHis: 0.512 ± 0.192
4.008ValIle: 4.008 ± 0.764
5.713ValLys: 5.713 ± 0.791
4.69ValLeu: 4.69 ± 0.625
1.705ValMet: 1.705 ± 0.326
4.605ValAsn: 4.605 ± 1.549
1.535ValPro: 1.535 ± 0.352
2.643ValGln: 2.643 ± 0.396
1.961ValArg: 1.961 ± 0.419
4.349ValSer: 4.349 ± 0.572
3.24ValThr: 3.24 ± 0.764
2.814ValVal: 2.814 ± 0.639
0.426ValTrp: 0.426 ± 0.16
2.302ValTyr: 2.302 ± 0.496
0.0ValXaa: 0.0 ± 0.0
Trp
0.597TrpAla: 0.597 ± 0.217
0.256TrpCys: 0.256 ± 0.161
0.426TrpAsp: 0.426 ± 0.186
0.767TrpGlu: 0.767 ± 0.255
0.0TrpPhe: 0.0 ± 0.0
0.597TrpGly: 0.597 ± 0.258
0.256TrpHis: 0.256 ± 0.15
1.023TrpIle: 1.023 ± 0.316
0.767TrpLys: 0.767 ± 0.275
1.279TrpLeu: 1.279 ± 0.323
0.0TrpMet: 0.0 ± 0.0
0.682TrpAsn: 0.682 ± 0.239
0.0TrpPro: 0.0 ± 0.0
0.256TrpGln: 0.256 ± 0.127
0.256TrpArg: 0.256 ± 0.187
0.853TrpSer: 0.853 ± 0.262
0.767TrpThr: 0.767 ± 0.271
0.256TrpVal: 0.256 ± 0.146
0.171TrpTrp: 0.171 ± 0.108
0.256TrpTyr: 0.256 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.132TyrAla: 2.132 ± 0.585
0.426TyrCys: 0.426 ± 0.176
2.132TyrAsp: 2.132 ± 0.454
2.302TyrGlu: 2.302 ± 0.485
1.364TyrPhe: 1.364 ± 0.392
2.643TyrGly: 2.643 ± 0.391
0.512TyrHis: 0.512 ± 0.199
2.388TyrIle: 2.388 ± 0.444
4.008TyrLys: 4.008 ± 0.785
3.752TyrLeu: 3.752 ± 0.545
0.256TyrMet: 0.256 ± 0.12
2.388TyrAsn: 2.388 ± 0.401
1.705TyrPro: 1.705 ± 0.525
2.132TyrGln: 2.132 ± 0.494
1.791TyrArg: 1.791 ± 0.423
2.899TyrSer: 2.899 ± 0.598
2.643TyrThr: 2.643 ± 0.51
1.876TyrVal: 1.876 ± 0.379
0.256TyrTrp: 0.256 ± 0.181
1.364TyrTyr: 1.364 ± 0.358
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11728 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski