Lactobacillus vini DSM 20605
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2C681|A0A0R2C681_9LACO Uncharacterized protein OS=Lactobacillus vini DSM 20605 OX=1133569 GN=FD21_GL001447 PE=4 SV=1
MM1 pKa = 6.35 EE2 pKa = 4.44 TVRR5 pKa = 11.84 VFIVNLGYY13 pKa = 10.8 YY14 pKa = 9.48 NQGKK18 pKa = 6.46 TTGKK22 pKa = 9.32 WFTPPLYY29 pKa = 9.43 PDD31 pKa = 5.54 AIAEE35 pKa = 4.01 QLEE38 pKa = 4.38 LKK40 pKa = 10.48 NDD42 pKa = 3.5 SEE44 pKa = 4.9 EE45 pKa = 4.28 YY46 pKa = 10.44 AIHH49 pKa = 7.62 DD50 pKa = 3.83 YY51 pKa = 9.66 EE52 pKa = 5.82 APFEE56 pKa = 4.18 IDD58 pKa = 3.38 RR59 pKa = 11.84 FDD61 pKa = 5.72 SIDD64 pKa = 3.83 EE65 pKa = 4.16 INRR68 pKa = 11.84 KK69 pKa = 8.59 YY70 pKa = 10.79 AALEE74 pKa = 3.7 RR75 pKa = 11.84 LEE77 pKa = 5.22 EE78 pKa = 4.16 YY79 pKa = 11.01 DD80 pKa = 4.14 FGADD84 pKa = 3.17 VSALIGEE91 pKa = 4.7 WFRR94 pKa = 11.84 DD95 pKa = 3.48 IEE97 pKa = 4.19 EE98 pKa = 4.21 LAEE101 pKa = 4.04 NAEE104 pKa = 4.84 DD105 pKa = 3.27 IVIYY109 pKa = 10.69 KK110 pKa = 10.11 GVSDD114 pKa = 4.06 MAEE117 pKa = 3.94 LAQDD121 pKa = 3.61 AVEE124 pKa = 4.5 SGAIFGDD131 pKa = 4.29 LPDD134 pKa = 3.66 QVIAYY139 pKa = 10.24 LDD141 pKa = 4.06 FEE143 pKa = 4.49 ALGRR147 pKa = 11.84 DD148 pKa = 3.37 IEE150 pKa = 4.34 IEE152 pKa = 3.99 GNYY155 pKa = 10.28 LVTNSAIYY163 pKa = 10.05 EE164 pKa = 4.22 YY165 pKa = 11.49 ANN167 pKa = 3.22
Molecular weight: 19.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.668
EMBOSS 3.694
Sillero 3.935
Patrickios 1.863
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A0R2CHI2|A0A0R2CHI2_9LACO Site-specific recombinase OS=Lactobacillus vini DSM 20605 OX=1133569 GN=FD21_GL001615 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 LTVKK6 pKa = 10.19 VVARR10 pKa = 11.84 LWRR13 pKa = 11.84 HH14 pKa = 4.35 IQVLQRR20 pKa = 11.84 GMQIQQAAANQARR33 pKa = 11.84 IQAQQQHH40 pKa = 5.05 QVVKK44 pKa = 10.79 VRR46 pKa = 11.84 ILAQLNRR53 pKa = 11.84 VQIQQAAVNQARR65 pKa = 11.84 IQAQRR70 pKa = 11.84 QRR72 pKa = 11.84 QVVKK76 pKa = 10.98 ARR78 pKa = 11.84 MLAQLNRR85 pKa = 11.84 VQIQQAAANQVRR97 pKa = 11.84 IQAQQQRR104 pKa = 11.84 QVVKK108 pKa = 11.01 ARR110 pKa = 11.84 MLAQLNRR117 pKa = 11.84 VQIQQAAANQVRR129 pKa = 11.84 IQAQQQRR136 pKa = 11.84 QVVKK140 pKa = 11.01 ARR142 pKa = 11.84 MLAQLNRR149 pKa = 11.84 VQIQQAAANQVRR161 pKa = 11.84 IQAQQQRR168 pKa = 11.84 QVVKK172 pKa = 11.01 ARR174 pKa = 11.84 MLAQLNRR181 pKa = 11.84 VQIQQAAANQVRR193 pKa = 11.84 TQAQRR198 pKa = 11.84 QHH200 pKa = 5.27 QVAKK204 pKa = 10.82 ARR206 pKa = 11.84 ILAQLNQVRR215 pKa = 11.84 IPQAAANQVRR225 pKa = 11.84 IQAQRR230 pKa = 11.84 QHH232 pKa = 5.3 QVAKK236 pKa = 10.94 ARR238 pKa = 11.84 MLAQLNRR245 pKa = 11.84 VRR247 pKa = 11.84 IPQAAANQVRR257 pKa = 11.84 IQAQRR262 pKa = 11.84 QCQVVKK268 pKa = 10.9 VRR270 pKa = 11.84 IQLII274 pKa = 3.74
Molecular weight: 31.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.462
IPC_protein 13.071
Toseland 13.232
ProMoST 13.729
Dawson 13.232
Bjellqvist 13.232
Wikipedia 13.715
Rodwell 12.837
Grimsley 13.276
Solomon 13.729
Lehninger 13.627
Nozaki 13.232
DTASelect 13.232
Thurlkill 13.232
EMBOSS 13.729
Sillero 13.232
Patrickios 12.544
IPC_peptide 13.729
IPC2_peptide 12.72
IPC2.peptide.svr19 9.349
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2108
0
2108
626100
44
1489
297.0
33.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.118 ± 0.055
0.657 ± 0.016
4.879 ± 0.041
5.01 ± 0.049
4.542 ± 0.045
6.391 ± 0.048
2.008 ± 0.022
7.431 ± 0.048
7.145 ± 0.05
10.92 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.306 ± 0.023
4.742 ± 0.035
3.578 ± 0.027
5.745 ± 0.057
3.934 ± 0.038
5.933 ± 0.058
5.582 ± 0.045
6.598 ± 0.047
1.041 ± 0.021
3.433 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here