Equus caballus papillomavirus 6
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4HXD4|M4HXD4_9PAPI Replication protein E1 OS=Equus caballus papillomavirus 6 OX=1235427 GN=E1 PE=3 SV=1
MM1 pKa = 7.47 LRR3 pKa = 11.84 ARR5 pKa = 11.84 PRR7 pKa = 11.84 NQNPEE12 pKa = 3.88 EE13 pKa = 4.2 SLSDD17 pKa = 3.8 DD18 pKa = 4.0 SGVEE22 pKa = 3.73 ADD24 pKa = 3.58 GSEE27 pKa = 4.32 TEE29 pKa = 4.75 EE30 pKa = 4.04 EE31 pKa = 4.23 QSPDD35 pKa = 3.11 RR36 pKa = 11.84 YY37 pKa = 9.47 PYY39 pKa = 10.42 RR40 pKa = 11.84 VLTFCFYY47 pKa = 10.59 CQLGVRR53 pKa = 11.84 IVLVSTSSGIRR64 pKa = 11.84 QINRR68 pKa = 11.84 LLTDD72 pKa = 3.69 SVAIVCPLCASARR85 pKa = 11.84 GYY87 pKa = 10.38 YY88 pKa = 10.14 GSRR91 pKa = 3.37
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.901
IPC2_protein 5.194
IPC_protein 4.94
Toseland 4.787
ProMoST 5.029
Dawson 4.863
Bjellqvist 5.016
Wikipedia 4.724
Rodwell 4.774
Grimsley 4.698
Solomon 4.863
Lehninger 4.813
Nozaki 4.978
DTASelect 5.105
Thurlkill 4.787
EMBOSS 4.736
Sillero 5.041
Patrickios 3.465
IPC_peptide 4.876
IPC2_peptide 5.029
IPC2.peptide.svr19 5.143
Protein with the highest isoelectric point:
>tr|M4HX85|M4HX85_9PAPI Protein E7 OS=Equus caballus papillomavirus 6 OX=1235427 PE=3 SV=1
HHH2 pKa = 7.16 KKK4 pKa = 10.3 KKK6 pKa = 10.23 HH7 pKa = 6.03 LPDDD11 pKa = 3.46 PRR13 pKa = 11.84 RR14 pKa = 11.84 TPEEE18 pKa = 3.67 LLTLAYYY25 pKa = 9.55 KK26 pKa = 10.51 ATKKK30 pKa = 8.49 TGRR33 pKa = 11.84 PDDD36 pKa = 4.46 IYYY39 pKa = 10.73 YY40 pKa = 9.73 GRR42 pKa = 11.84 LAPRR46 pKa = 11.84 LLVSDDD52 pKa = 4.52 YYY54 pKa = 11.37 RR55 pKa = 11.84 TSYYY59 pKa = 10.97 EEE61 pKa = 3.68 RR62 pKa = 11.84 RR63 pKa = 11.84 GIAEEE68 pKa = 4.11 LTEEE72 pKa = 4.71 SPLGAQGALPMSPIPGPYYY91 pKa = 8.93 AGYYY95 pKa = 9.32 EEE97 pKa = 5.67 YYY99 pKa = 10.05 PPAPCRR105 pKa = 11.84 RR106 pKa = 11.84 LYYY109 pKa = 10.22 AHHH112 pKa = 7.69 YYY114 pKa = 9.71 HH115 pKa = 6.21 RR116 pKa = 11.84 QVYYY120 pKa = 9.81 LYYY123 pKa = 10.08 QYYY126 pKa = 9.33 QDDD129 pKa = 4.83 PAGPPVPPLQ
Molecular weight: 15.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.702
IPC2_protein 9.545
IPC_protein 9.94
Toseland 9.385
ProMoST 9.458
Dawson 9.794
Bjellqvist 9.663
Wikipedia 10.101
Rodwell 9.823
Grimsley 9.955
Solomon 9.853
Lehninger 9.78
Nozaki 9.472
DTASelect 9.633
Thurlkill 9.575
EMBOSS 9.853
Sillero 9.736
Patrickios 3.834
IPC_peptide 9.823
IPC2_peptide 8.741
IPC2.peptide.svr19 8.235
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2423
91
617
346.1
38.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.768 ± 0.609
2.518 ± 0.709
5.695 ± 0.37
6.067 ± 0.246
3.797 ± 0.312
7.099 ± 1.1
1.981 ± 0.195
3.095 ± 0.423
4.333 ± 0.916
9.451 ± 0.675
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.403 ± 0.332
2.848 ± 0.41
7.511 ± 0.894
4.499 ± 0.503
7.305 ± 0.438
7.305 ± 0.449
6.397 ± 0.44
6.521 ± 0.774
1.733 ± 0.448
3.673 ± 0.584
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here