Dermabacter sp. HFH0086
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S3YAR8|S3YAR8_9MICO Uncharacterized protein OS=Dermabacter sp. HFH0086 OX=1203568 GN=HMPREF1484_01977 PE=4 SV=1
MM1 pKa = 7.2 PTGPKK6 pKa = 8.71 HH7 pKa = 5.43 WKK9 pKa = 7.22 HH10 pKa = 5.7 TEE12 pKa = 3.93 VGDD15 pKa = 4.05 GTDD18 pKa = 3.51 DD19 pKa = 3.16 TWAEE23 pKa = 4.11 EE24 pKa = 4.37 CRR26 pKa = 11.84 CMIGEE31 pKa = 4.11 DD32 pKa = 3.88 HH33 pKa = 6.92 YY34 pKa = 11.95 VGADD38 pKa = 3.29 SPMSEE43 pKa = 4.29 MLSEE47 pKa = 4.12 VDD49 pKa = 5.21 AEE51 pKa = 4.83 DD52 pKa = 4.27 IYY54 pKa = 11.31 LSSGMDD60 pKa = 3.42 PDD62 pKa = 3.71 YY63 pKa = 11.43 DD64 pKa = 3.73 FRR66 pKa = 6.61
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.986
IPC_protein 3.923
Toseland 3.732
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.05
Patrickios 1.926
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.93
Protein with the highest isoelectric point:
>tr|S3ZBV9|S3ZBV9_9MICO Aamy domain-containing protein OS=Dermabacter sp. HFH0086 OX=1203568 GN=HMPREF1484_00147 PE=4 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.09 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 10.04 KK16 pKa = 8.86 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIINARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.75 GRR40 pKa = 11.84 ASLSAA45 pKa = 3.83
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2120
0
2120
711070
28
2235
335.4
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.126 ± 0.068
0.675 ± 0.014
5.861 ± 0.043
6.82 ± 0.056
3.318 ± 0.038
8.41 ± 0.046
2.212 ± 0.025
4.836 ± 0.037
3.237 ± 0.048
9.727 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.022 ± 0.021
2.562 ± 0.032
5.069 ± 0.044
2.745 ± 0.031
6.784 ± 0.065
6.174 ± 0.034
6.047 ± 0.047
7.962 ± 0.045
1.32 ± 0.021
2.094 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here