Amino acid dipepetide frequency for Dermabacter sp. HFH0086

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.838AlaAla: 14.838 ± 0.211
0.951AlaCys: 0.951 ± 0.039
6.341AlaAsp: 6.341 ± 0.1
7.694AlaGlu: 7.694 ± 0.123
3.994AlaPhe: 3.994 ± 0.095
9.951AlaGly: 9.951 ± 0.126
3.091AlaHis: 3.091 ± 0.086
5.894AlaIle: 5.894 ± 0.104
4.285AlaLys: 4.285 ± 0.102
14.049AlaLeu: 14.049 ± 0.186
2.728AlaMet: 2.728 ± 0.074
2.728AlaAsn: 2.728 ± 0.067
5.874AlaPro: 5.874 ± 0.151
3.704AlaGln: 3.704 ± 0.073
8.815AlaArg: 8.815 ± 0.151
7.477AlaSer: 7.477 ± 0.113
6.314AlaThr: 6.314 ± 0.089
8.525AlaVal: 8.525 ± 0.125
1.622AlaTrp: 1.622 ± 0.058
2.381AlaTyr: 2.381 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.903CysAla: 0.903 ± 0.04
0.046CysCys: 0.046 ± 0.008
0.388CysAsp: 0.388 ± 0.024
0.53CysGlu: 0.53 ± 0.03
0.201CysPhe: 0.201 ± 0.017
0.818CysGly: 0.818 ± 0.037
0.153CysHis: 0.153 ± 0.015
0.269CysIle: 0.269 ± 0.02
0.111CysLys: 0.111 ± 0.013
0.567CysLeu: 0.567 ± 0.029
0.117CysMet: 0.117 ± 0.012
0.148CysAsn: 0.148 ± 0.015
0.345CysPro: 0.345 ± 0.022
0.156CysGln: 0.156 ± 0.016
0.401CysArg: 0.401 ± 0.025
0.42CysSer: 0.42 ± 0.027
0.443CysThr: 0.443 ± 0.026
0.527CysVal: 0.527 ± 0.026
0.065CysTrp: 0.065 ± 0.01
0.141CysTyr: 0.141 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
7.13AspAla: 7.13 ± 0.109
0.316AspCys: 0.316 ± 0.021
3.554AspAsp: 3.554 ± 0.075
4.264AspGlu: 4.264 ± 0.096
1.935AspPhe: 1.935 ± 0.057
5.106AspGly: 5.106 ± 0.126
1.27AspHis: 1.27 ± 0.045
2.872AspIle: 2.872 ± 0.062
1.546AspLys: 1.546 ± 0.062
5.959AspLeu: 5.959 ± 0.101
1.125AspMet: 1.125 ± 0.041
1.266AspAsn: 1.266 ± 0.053
3.844AspPro: 3.844 ± 0.075
1.537AspGln: 1.537 ± 0.052
3.686AspArg: 3.686 ± 0.068
3.322AspSer: 3.322 ± 0.071
3.018AspThr: 3.018 ± 0.068
4.859AspVal: 4.859 ± 0.105
0.685AspTrp: 0.685 ± 0.031
1.315AspTyr: 1.315 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
8.313GluAla: 8.313 ± 0.129
0.433GluCys: 0.433 ± 0.025
3.617GluAsp: 3.617 ± 0.076
4.578GluGlu: 4.578 ± 0.094
2.084GluPhe: 2.084 ± 0.053
5.606GluGly: 5.606 ± 0.112
1.793GluHis: 1.793 ± 0.06
3.465GluIle: 3.465 ± 0.075
2.898GluLys: 2.898 ± 0.078
6.442GluLeu: 6.442 ± 0.115
1.173GluMet: 1.173 ± 0.042
2.09GluAsn: 2.09 ± 0.054
3.098GluPro: 3.098 ± 0.093
2.365GluGln: 2.365 ± 0.061
5.871GluArg: 5.871 ± 0.117
3.751GluSer: 3.751 ± 0.07
3.53GluThr: 3.53 ± 0.067
4.841GluVal: 4.841 ± 0.089
0.893GluTrp: 0.893 ± 0.035
1.354GluTyr: 1.354 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
4.13PheAla: 4.13 ± 0.096
0.217PheCys: 0.217 ± 0.018
2.368PheAsp: 2.368 ± 0.062
2.257PheGlu: 2.257 ± 0.057
1.208PhePhe: 1.208 ± 0.052
3.206PheGly: 3.206 ± 0.081
0.672PheHis: 0.672 ± 0.036
1.351PheIle: 1.351 ± 0.047
0.776PheLys: 0.776 ± 0.038
2.97PheLeu: 2.97 ± 0.072
0.617PheMet: 0.617 ± 0.031
0.811PheAsn: 0.811 ± 0.032
1.408PhePro: 1.408 ± 0.049
0.831PheGln: 0.831 ± 0.031
1.651PheArg: 1.651 ± 0.051
2.159PheSer: 2.159 ± 0.057
2.617PheThr: 2.617 ± 0.059
2.801PheVal: 2.801 ± 0.07
0.432PheTrp: 0.432 ± 0.026
0.692PheTyr: 0.692 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
9.964GlyAla: 9.964 ± 0.139
0.661GlyCys: 0.661 ± 0.037
4.687GlyAsp: 4.687 ± 0.076
6.051GlyGlu: 6.051 ± 0.116
3.108GlyPhe: 3.108 ± 0.073
6.905GlyGly: 6.905 ± 0.136
1.81GlyHis: 1.81 ± 0.054
4.656GlyIle: 4.656 ± 0.081
3.181GlyLys: 3.181 ± 0.091
7.688GlyLeu: 7.688 ± 0.123
1.876GlyMet: 1.876 ± 0.052
2.093GlyAsn: 2.093 ± 0.057
3.244GlyPro: 3.244 ± 0.069
2.346GlyGln: 2.346 ± 0.065
5.222GlyArg: 5.222 ± 0.084
5.292GlySer: 5.292 ± 0.113
5.393GlyThr: 5.393 ± 0.088
6.579GlyVal: 6.579 ± 0.103
1.187GlyTrp: 1.187 ± 0.045
2.155GlyTyr: 2.155 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
2.562HisAla: 2.562 ± 0.069
0.145HisCys: 0.145 ± 0.015
1.385HisAsp: 1.385 ± 0.041
1.681HisGlu: 1.681 ± 0.051
0.668HisPhe: 0.668 ± 0.029
1.906HisGly: 1.906 ± 0.07
0.556HisHis: 0.556 ± 0.028
0.892HisIle: 0.892 ± 0.035
0.515HisLys: 0.515 ± 0.027
2.114HisLeu: 2.114 ± 0.061
0.489HisMet: 0.489 ± 0.029
0.52HisAsn: 0.52 ± 0.025
1.384HisPro: 1.384 ± 0.044
0.522HisGln: 0.522 ± 0.03
1.46HisArg: 1.46 ± 0.044
1.254HisSer: 1.254 ± 0.04
1.371HisThr: 1.371 ± 0.038
1.906HisVal: 1.906 ± 0.049
0.243HisTrp: 0.243 ± 0.02
0.548HisTyr: 0.548 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.891IleAla: 6.891 ± 0.118
0.266IleCys: 0.266 ± 0.018
3.877IleAsp: 3.877 ± 0.071
3.375IleGlu: 3.375 ± 0.081
1.384IlePhe: 1.384 ± 0.046
4.119IleGly: 4.119 ± 0.084
0.882IleHis: 0.882 ± 0.038
2.128IleIle: 2.128 ± 0.056
1.159IleLys: 1.159 ± 0.039
4.336IleLeu: 4.336 ± 0.101
0.859IleMet: 0.859 ± 0.034
1.212IleAsn: 1.212 ± 0.044
2.756IlePro: 2.756 ± 0.054
1.089IleGln: 1.089 ± 0.04
2.605IleArg: 2.605 ± 0.064
2.697IleSer: 2.697 ± 0.053
2.862IleThr: 2.862 ± 0.063
4.687IleVal: 4.687 ± 0.095
0.38IleTrp: 0.38 ± 0.024
0.799IleTyr: 0.799 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
4.07LysAla: 4.07 ± 0.091
0.145LysCys: 0.145 ± 0.016
2.025LysAsp: 2.025 ± 0.066
1.906LysGlu: 1.906 ± 0.058
0.781LysPhe: 0.781 ± 0.029
2.723LysGly: 2.723 ± 0.076
0.653LysHis: 0.653 ± 0.03
1.406LysIle: 1.406 ± 0.052
1.794LysLys: 1.794 ± 0.075
2.71LysLeu: 2.71 ± 0.076
0.612LysMet: 0.612 ± 0.03
1.153LysAsn: 1.153 ± 0.038
1.841LysPro: 1.841 ± 0.074
0.911LysGln: 0.911 ± 0.037
2.409LysArg: 2.409 ± 0.058
1.869LysSer: 1.869 ± 0.057
1.79LysThr: 1.79 ± 0.059
2.475LysVal: 2.475 ± 0.063
0.37LysTrp: 0.37 ± 0.025
0.727LysTyr: 0.727 ± 0.035
0.0LysXaa: 0.0 ± 0.0
Leu
13.567LeuAla: 13.567 ± 0.195
0.598LeuCys: 0.598 ± 0.035
6.222LeuAsp: 6.222 ± 0.115
6.909LeuGlu: 6.909 ± 0.118
3.043LeuPhe: 3.043 ± 0.075
8.186LeuGly: 8.186 ± 0.135
1.899LeuHis: 1.899 ± 0.048
4.459LeuIle: 4.459 ± 0.097
2.692LeuLys: 2.692 ± 0.065
8.583LeuLeu: 8.583 ± 0.145
1.921LeuMet: 1.921 ± 0.06
2.357LeuAsn: 2.357 ± 0.058
5.0LeuPro: 5.0 ± 0.094
2.08LeuGln: 2.08 ± 0.055
6.037LeuArg: 6.037 ± 0.104
6.16LeuSer: 6.16 ± 0.1
6.621LeuThr: 6.621 ± 0.106
8.248LeuVal: 8.248 ± 0.163
1.101LeuTrp: 1.101 ± 0.05
1.586LeuTyr: 1.586 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.117MetAla: 2.117 ± 0.057
0.179MetCys: 0.179 ± 0.015
1.013MetAsp: 1.013 ± 0.036
0.765MetGlu: 0.765 ± 0.035
0.696MetPhe: 0.696 ± 0.039
1.522MetGly: 1.522 ± 0.047
0.458MetHis: 0.458 ± 0.028
1.004MetIle: 1.004 ± 0.04
0.647MetLys: 0.647 ± 0.034
2.008MetLeu: 2.008 ± 0.052
0.388MetMet: 0.388 ± 0.023
0.804MetAsn: 0.804 ± 0.03
1.112MetPro: 1.112 ± 0.041
0.581MetGln: 0.581 ± 0.026
1.585MetArg: 1.585 ± 0.045
1.759MetSer: 1.759 ± 0.054
1.553MetThr: 1.553 ± 0.05
1.394MetVal: 1.394 ± 0.048
0.274MetTrp: 0.274 ± 0.019
0.364MetTyr: 0.364 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.074AsnAla: 3.074 ± 0.061
0.136AsnCys: 0.136 ± 0.014
1.499AsnAsp: 1.499 ± 0.048
1.638AsnGlu: 1.638 ± 0.057
0.792AsnPhe: 0.792 ± 0.035
2.274AsnGly: 2.274 ± 0.078
0.488AsnHis: 0.488 ± 0.027
1.162AsnIle: 1.162 ± 0.041
0.71AsnLys: 0.71 ± 0.032
2.429AsnLeu: 2.429 ± 0.062
0.515AsnMet: 0.515 ± 0.026
0.653AsnAsn: 0.653 ± 0.035
1.942AsnPro: 1.942 ± 0.056
0.766AsnGln: 0.766 ± 0.033
1.546AsnArg: 1.546 ± 0.053
1.478AsnSer: 1.478 ± 0.048
1.515AsnThr: 1.515 ± 0.047
2.011AsnVal: 2.011 ± 0.048
0.353AsnTrp: 0.353 ± 0.024
0.638AsnTyr: 0.638 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
5.961ProAla: 5.961 ± 0.131
0.243ProCys: 0.243 ± 0.017
2.852ProAsp: 2.852 ± 0.064
4.485ProGlu: 4.485 ± 0.096
1.695ProPhe: 1.695 ± 0.045
4.538ProGly: 4.538 ± 0.102
1.287ProHis: 1.287 ± 0.044
2.223ProIle: 2.223 ± 0.057
1.693ProLys: 1.693 ± 0.057
4.677ProLeu: 4.677 ± 0.094
0.953ProMet: 0.953 ± 0.035
1.38ProAsn: 1.38 ± 0.049
1.794ProPro: 1.794 ± 0.06
1.468ProGln: 1.468 ± 0.059
3.076ProArg: 3.076 ± 0.082
3.526ProSer: 3.526 ± 0.082
3.178ProThr: 3.178 ± 0.076
3.865ProVal: 3.865 ± 0.084
0.709ProTrp: 0.709 ± 0.032
1.087ProTyr: 1.087 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
3.235GlnAla: 3.235 ± 0.079
0.179GlnCys: 0.179 ± 0.018
1.247GlnAsp: 1.247 ± 0.04
1.513GlnGlu: 1.513 ± 0.058
0.994GlnPhe: 0.994 ± 0.041
2.226GlnGly: 2.226 ± 0.054
0.637GlnHis: 0.637 ± 0.031
1.536GlnIle: 1.536 ± 0.043
1.034GlnLys: 1.034 ± 0.045
2.716GlnLeu: 2.716 ± 0.069
0.631GlnMet: 0.631 ± 0.031
0.692GlnAsn: 0.692 ± 0.032
1.449GlnPro: 1.449 ± 0.053
1.08GlnGln: 1.08 ± 0.049
2.126GlnArg: 2.126 ± 0.06
1.531GlnSer: 1.531 ± 0.043
1.263GlnThr: 1.263 ± 0.045
2.205GlnVal: 2.205 ± 0.051
0.474GlnTrp: 0.474 ± 0.027
0.686GlnTyr: 0.686 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
8.23ArgAla: 8.23 ± 0.14
0.367ArgCys: 0.367 ± 0.023
3.936ArgAsp: 3.936 ± 0.082
5.928ArgGlu: 5.928 ± 0.113
2.259ArgPhe: 2.259 ± 0.062
5.022ArgGly: 5.022 ± 0.097
1.439ArgHis: 1.439 ± 0.042
3.446ArgIle: 3.446 ± 0.075
2.034ArgLys: 2.034 ± 0.06
6.288ArgLeu: 6.288 ± 0.11
1.592ArgMet: 1.592 ± 0.043
1.467ArgAsn: 1.467 ± 0.05
3.001ArgPro: 3.001 ± 0.068
1.74ArgGln: 1.74 ± 0.056
5.153ArgArg: 5.153 ± 0.107
3.907ArgSer: 3.907 ± 0.084
4.372ArgThr: 4.372 ± 0.083
5.3ArgVal: 5.3 ± 0.092
0.862ArgTrp: 0.862 ± 0.03
1.496ArgTyr: 1.496 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
7.288SerAla: 7.288 ± 0.117
0.383SerCys: 0.383 ± 0.026
3.53SerAsp: 3.53 ± 0.076
3.839SerGlu: 3.839 ± 0.083
2.27SerPhe: 2.27 ± 0.061
5.614SerGly: 5.614 ± 0.094
1.311SerHis: 1.311 ± 0.039
3.012SerIle: 3.012 ± 0.068
1.828SerLys: 1.828 ± 0.063
5.981SerLeu: 5.981 ± 0.096
1.354SerMet: 1.354 ± 0.044
1.55SerAsn: 1.55 ± 0.053
3.228SerPro: 3.228 ± 0.07
1.661SerGln: 1.661 ± 0.055
4.202SerArg: 4.202 ± 0.083
4.132SerSer: 4.132 ± 0.083
3.775SerThr: 3.775 ± 0.075
4.676SerVal: 4.676 ± 0.084
0.848SerTrp: 0.848 ± 0.037
1.259SerTyr: 1.259 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
6.261ThrAla: 6.261 ± 0.095
0.439ThrCys: 0.439 ± 0.026
3.225ThrAsp: 3.225 ± 0.069
3.444ThrGlu: 3.444 ± 0.076
2.15ThrPhe: 2.15 ± 0.061
5.203ThrGly: 5.203 ± 0.097
1.429ThrHis: 1.429 ± 0.044
2.974ThrIle: 2.974 ± 0.063
1.946ThrLys: 1.946 ± 0.061
6.31ThrLeu: 6.31 ± 0.097
1.098ThrMet: 1.098 ± 0.045
1.56ThrAsn: 1.56 ± 0.047
3.756ThrPro: 3.756 ± 0.077
1.558ThrGln: 1.558 ± 0.05
3.862ThrArg: 3.862 ± 0.077
3.997ThrSer: 3.997 ± 0.073
3.572ThrThr: 3.572 ± 0.088
5.268ThrVal: 5.268 ± 0.096
0.987ThrTrp: 0.987 ± 0.035
1.433ThrTyr: 1.433 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
8.891ValAla: 8.891 ± 0.121
0.736ValCys: 0.736 ± 0.032
4.804ValAsp: 4.804 ± 0.085
5.164ValGlu: 5.164 ± 0.088
2.733ValPhe: 2.733 ± 0.072
6.334ValGly: 6.334 ± 0.127
1.665ValHis: 1.665 ± 0.053
4.125ValIle: 4.125 ± 0.07
2.545ValLys: 2.545 ± 0.065
8.041ValLeu: 8.041 ± 0.123
1.51ValMet: 1.51 ± 0.048
2.162ValAsn: 2.162 ± 0.059
3.973ValPro: 3.973 ± 0.088
1.97ValGln: 1.97 ± 0.055
5.613ValArg: 5.613 ± 0.1
4.867ValSer: 4.867 ± 0.096
5.213ValThr: 5.213 ± 0.101
6.649ValVal: 6.649 ± 0.117
1.104ValTrp: 1.104 ± 0.04
1.519ValTyr: 1.519 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.401TrpAla: 1.401 ± 0.045
0.131TrpCys: 0.131 ± 0.016
0.781TrpAsp: 0.781 ± 0.043
0.734TrpGlu: 0.734 ± 0.034
0.419TrpPhe: 0.419 ± 0.028
0.979TrpGly: 0.979 ± 0.04
0.304TrpHis: 0.304 ± 0.02
0.723TrpIle: 0.723 ± 0.031
0.416TrpLys: 0.416 ± 0.025
1.35TrpLeu: 1.35 ± 0.048
0.288TrpMet: 0.288 ± 0.019
0.425TrpAsn: 0.425 ± 0.026
0.591TrpPro: 0.591 ± 0.03
0.487TrpGln: 0.487 ± 0.026
0.982TrpArg: 0.982 ± 0.037
0.779TrpSer: 0.779 ± 0.032
0.782TrpThr: 0.782 ± 0.033
1.043TrpVal: 1.043 ± 0.04
0.319TrpTrp: 0.319 ± 0.02
0.264TrpTyr: 0.264 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.433TyrAla: 2.433 ± 0.056
0.18TyrCys: 0.18 ± 0.017
1.256TyrAsp: 1.256 ± 0.048
1.44TyrGlu: 1.44 ± 0.045
0.764TyrPhe: 0.764 ± 0.035
1.859TyrGly: 1.859 ± 0.057
0.325TyrHis: 0.325 ± 0.023
0.765TyrIle: 0.765 ± 0.033
0.558TyrLys: 0.558 ± 0.028
2.05TyrLeu: 2.05 ± 0.057
0.373TyrMet: 0.373 ± 0.022
0.55TyrAsn: 0.55 ± 0.03
1.052TyrPro: 1.052 ± 0.044
0.602TyrGln: 0.602 ± 0.029
1.539TyrArg: 1.539 ± 0.047
1.361TyrSer: 1.361 ± 0.044
1.291TyrThr: 1.291 ± 0.049
1.758TyrVal: 1.758 ± 0.048
0.29TyrTrp: 0.29 ± 0.021
0.494TyrTyr: 0.494 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2120 proteins (711070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski