Tatumella morbirosei
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3697 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A095TEG5|A0A095TEG5_9GAMM Uncharacterized protein OS=Tatumella morbirosei OX=642227 GN=HA49_06490 PE=4 SV=1
MM1 pKa = 7.81 KK2 pKa = 10.19 IGLFYY7 pKa = 10.96 GSSTCYY13 pKa = 10.19 TEE15 pKa = 4.84 IVAEE19 pKa = 4.59 KK20 pKa = 10.16 IRR22 pKa = 11.84 DD23 pKa = 4.11 FIGADD28 pKa = 3.5 LVTLHH33 pKa = 5.95 NVKK36 pKa = 10.76 DD37 pKa = 3.84 GTTEE41 pKa = 3.75 SMQYY45 pKa = 10.92 YY46 pKa = 10.25 DD47 pKa = 4.9 LLIMGIPTWDD57 pKa = 3.41 FGEE60 pKa = 4.35 LQEE63 pKa = 4.58 DD64 pKa = 4.38 WEE66 pKa = 5.36 AIWPMLPDD74 pKa = 3.98 LQLQGKK80 pKa = 8.73 IVALYY85 pKa = 11.06 GMGDD89 pKa = 3.46 QIGYY93 pKa = 8.42 SEE95 pKa = 4.58 WFLDD99 pKa = 4.0 ALGMLHH105 pKa = 7.65 DD106 pKa = 5.49 LLQPMGVRR114 pKa = 11.84 FIGYY118 pKa = 7.91 WPTEE122 pKa = 3.78 GFEE125 pKa = 4.74 FISQKK130 pKa = 10.93 AVVPGGSQFVGLALDD145 pKa = 4.41 DD146 pKa = 4.14 VNQFEE151 pKa = 4.52 QTDD154 pKa = 3.56 EE155 pKa = 6.03 RR156 pKa = 11.84 IEE158 pKa = 3.98 QWCEE162 pKa = 3.55 QILTEE167 pKa = 4.99 MSDD170 pKa = 3.57 LLL172 pKa = 4.68
Molecular weight: 19.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.668
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.694
Grimsley 3.579
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.986
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A095V0Z6|A0A095V0Z6_9GAMM Uncharacterized protein OS=Tatumella morbirosei OX=642227 GN=HA49_00455 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3697
0
3697
1154041
29
1484
312.2
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.443 ± 0.049
1.073 ± 0.014
5.056 ± 0.034
5.323 ± 0.04
3.844 ± 0.027
7.496 ± 0.034
2.309 ± 0.019
5.908 ± 0.031
3.874 ± 0.033
11.011 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.02
3.606 ± 0.026
4.637 ± 0.026
5.037 ± 0.04
5.648 ± 0.033
6.545 ± 0.035
5.398 ± 0.03
6.92 ± 0.033
1.45 ± 0.016
2.8 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here