Rickettsia amblyommatis str. Darkwater

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group; Rickettsia amblyommatis

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1902 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F3R2A6|A0A0F3R2A6_RICAM Uncharacterized protein (Fragment) OS=Rickettsia amblyommatis str. Darkwater OX=1359166 GN=RAMDARK_0170 PE=4 SV=1
MM1 pKa = 8.29DD2 pKa = 4.74DD3 pKa = 4.2HH4 pKa = 7.04NISALSFDD12 pKa = 3.18ITAEE16 pKa = 4.04QKK18 pKa = 10.16EE19 pKa = 3.89ALINSGYY26 pKa = 10.48SATCDD31 pKa = 2.99YY32 pKa = 8.37VTRR35 pKa = 11.84MEE37 pKa = 4.77NIMLAGLGVNDD48 pKa = 4.68SNDD51 pKa = 3.55SLVLL55 pKa = 3.71

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F3QF98|A0A0F3QF98_RICAM UbiE/COQ5 methyltransferase family protein OS=Rickettsia amblyommatis str. Darkwater OX=1359166 GN=RAMDARK_1126 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPTGRR28 pKa = 11.84AILRR32 pKa = 11.84KK33 pKa = 8.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84HH40 pKa = 5.49KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1902

0

1902

370007

30

2228

194.5

21.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.491 ± 0.077

1.06 ± 0.027

4.977 ± 0.04

6.188 ± 0.068

4.575 ± 0.058

5.353 ± 0.073

1.886 ± 0.029

9.762 ± 0.074

8.427 ± 0.071

10.08 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.031

6.435 ± 0.065

3.278 ± 0.053

3.356 ± 0.045

3.521 ± 0.047

6.87 ± 0.055

5.244 ± 0.064

5.575 ± 0.053

0.781 ± 0.02

3.81 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski