Caldicoprobacter faecalis
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2413 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I5T977|A0A1I5T977_9FIRM FemAB family protein OS=Caldicoprobacter faecalis OX=937334 GN=SAMN05444406_10413 PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.56 AHH4 pKa = 6.51 VDD6 pKa = 3.02 KK7 pKa = 10.11 DD8 pKa = 3.72 TCIGCGLCADD18 pKa = 4.2 ICPEE22 pKa = 4.13 VFSMDD27 pKa = 3.99 DD28 pKa = 3.6 DD29 pKa = 4.37 GLAVAIDD36 pKa = 3.55 ADD38 pKa = 3.86 IPEE41 pKa = 5.09 DD42 pKa = 3.83 ALDD45 pKa = 3.72 SAKK48 pKa = 10.65 DD49 pKa = 3.71 AEE51 pKa = 4.45 QQCPSGAISIEE62 pKa = 4.0
Molecular weight: 6.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.605
IPC_protein 3.567
Toseland 3.35
ProMoST 3.745
Dawson 3.592
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.401
Grimsley 3.274
Solomon 3.554
Lehninger 3.516
Nozaki 3.732
DTASelect 3.973
Thurlkill 3.439
EMBOSS 3.579
Sillero 3.706
Patrickios 0.693
IPC_peptide 3.554
IPC2_peptide 3.668
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A1I5X7J7|A0A1I5X7J7_9FIRM Xylan alpha-1 2-glucuronidase OS=Caldicoprobacter faecalis OX=937334 GN=SAMN05444406_1235 PE=3 SV=1
MM1 pKa = 7.46 LRR3 pKa = 11.84 TYY5 pKa = 10.53 QPNNRR10 pKa = 11.84 KK11 pKa = 9.22 RR12 pKa = 11.84 KK13 pKa = 8.49 KK14 pKa = 8.63 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 KK20 pKa = 9.98 RR21 pKa = 11.84 MSTKK25 pKa = 9.78 SGRR28 pKa = 11.84 MVIKK32 pKa = 10.44 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.16 KK37 pKa = 9.91 GRR39 pKa = 11.84 KK40 pKa = 8.98 VLTAA44 pKa = 4.27
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2413
0
2413
730157
25
2868
302.6
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.128 ± 0.049
1.15 ± 0.022
5.388 ± 0.034
7.309 ± 0.059
4.115 ± 0.039
7.212 ± 0.05
1.755 ± 0.023
8.076 ± 0.047
6.648 ± 0.051
9.353 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.794 ± 0.022
4.05 ± 0.033
3.929 ± 0.032
3.304 ± 0.029
5.338 ± 0.038
5.262 ± 0.036
4.491 ± 0.028
7.725 ± 0.05
1.016 ± 0.019
3.958 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here