Hubei odonate virus 11
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KMU3|A0A1L3KMU3_9VIRU Uncharacterized protein OS=Hubei odonate virus 11 OX=1922992 PE=4 SV=1
MM1 pKa = 7.29 YY2 pKa = 9.65 IKK4 pKa = 10.58 KK5 pKa = 9.84 IFWFISLTLCMLEE18 pKa = 3.65 ISGLIGFDD26 pKa = 3.8 CKK28 pKa = 10.91 HH29 pKa = 6.67 KK30 pKa = 10.56 DD31 pKa = 3.43 VEE33 pKa = 4.39 YY34 pKa = 10.78 SAVSVKK40 pKa = 9.8 QVAEE44 pKa = 3.98 CDD46 pKa = 3.52 EE47 pKa = 4.55 VKK49 pKa = 10.54 PAVHH53 pKa = 5.57 QSKK56 pKa = 10.21 MNIQLLQKK64 pKa = 10.21 RR65 pKa = 11.84 RR66 pKa = 11.84 LSKK69 pKa = 10.66 VPYY72 pKa = 9.13 ISCYY76 pKa = 6.35 THH78 pKa = 6.73 ANILITHH85 pKa = 7.1 CGMHH89 pKa = 5.47 SHH91 pKa = 6.8 ASVVAGGLSQRR102 pKa = 11.84 IEE104 pKa = 4.06 QYY106 pKa = 10.28 TSEE109 pKa = 4.08 TCRR112 pKa = 11.84 RR113 pKa = 11.84 AFATKK118 pKa = 9.4 TVILFGTPIDD128 pKa = 3.53 INSFNTTITGSIIPYY143 pKa = 10.01 GSVINNDD150 pKa = 3.23 ASCIGTSFAYY160 pKa = 10.47 NGNHH164 pKa = 4.79 YY165 pKa = 9.41 RR166 pKa = 11.84 ASVMQVSYY174 pKa = 10.77 EE175 pKa = 4.19 IILKK179 pKa = 8.85 TGYY182 pKa = 8.36 GTYY185 pKa = 10.47 DD186 pKa = 3.28 SEE188 pKa = 4.38 TGIFNTGLGSYY199 pKa = 10.52 GDD201 pKa = 3.84 YY202 pKa = 11.03 KK203 pKa = 11.06 EE204 pKa = 5.59 GVLLDD209 pKa = 3.87 RR210 pKa = 11.84 NFGYY214 pKa = 10.15 VFWKK218 pKa = 9.8 TSDD221 pKa = 4.18 NEE223 pKa = 4.39 DD224 pKa = 3.81 CSSSTSYY231 pKa = 11.04 LVLYY235 pKa = 8.17 EE236 pKa = 4.6 GPAEE240 pKa = 4.09 KK241 pKa = 10.4 LVSEE245 pKa = 4.47 RR246 pKa = 11.84 DD247 pKa = 3.3 NRR249 pKa = 11.84 SIIVVEE255 pKa = 4.14 QQSTTFSLQLRR266 pKa = 11.84 EE267 pKa = 4.04 PTLLCSQHH275 pKa = 6.36 AFNTEE280 pKa = 3.35 HH281 pKa = 7.08 DD282 pKa = 3.72 QLKK285 pKa = 10.54 IITSNNRR292 pKa = 11.84 MFFFKK297 pKa = 10.61 RR298 pKa = 11.84 IADD301 pKa = 3.73 SLDD304 pKa = 3.43 INLSTYY310 pKa = 10.55 INAKK314 pKa = 8.1 FVYY317 pKa = 9.78 VEE319 pKa = 3.55 RR320 pKa = 11.84 HH321 pKa = 4.48 LRR323 pKa = 11.84 ANIEE327 pKa = 4.05 TLYY330 pKa = 10.44 EE331 pKa = 3.87 DD332 pKa = 3.63 VMKK335 pKa = 10.35 FRR337 pKa = 11.84 CRR339 pKa = 11.84 VTRR342 pKa = 11.84 QTLTNLLSLASIDD355 pKa = 3.72 EE356 pKa = 4.49 DD357 pKa = 3.75 EE358 pKa = 4.31 FAYY361 pKa = 10.29 AYY363 pKa = 8.77 MGRR366 pKa = 11.84 PGYY369 pKa = 9.38 TSVKK373 pKa = 9.14 RR374 pKa = 11.84 GEE376 pKa = 3.79 VAYY379 pKa = 10.31 LIKK382 pKa = 10.62 CVPTYY387 pKa = 9.59 ITLQSSTRR395 pKa = 11.84 CYY397 pKa = 8.83 NDD399 pKa = 3.06 VVISHH404 pKa = 5.84 NNKK407 pKa = 8.77 TKK409 pKa = 10.59 FLASKK414 pKa = 9.43 TNIIQDD420 pKa = 3.41 FSEE423 pKa = 4.29 EE424 pKa = 4.43 VEE426 pKa = 4.45 CSQLTPVLYY435 pKa = 10.16 EE436 pKa = 4.79 LQGKK440 pKa = 8.03 WYY442 pKa = 10.12 NVYY445 pKa = 9.69 PSPIAVPDD453 pKa = 4.85 PIVLNPNRR461 pKa = 11.84 EE462 pKa = 4.08 RR463 pKa = 11.84 EE464 pKa = 4.21 WEE466 pKa = 4.3 YY467 pKa = 11.26 KK468 pKa = 10.38 SPNHH472 pKa = 6.2 LSKK475 pKa = 10.98 SGIYY479 pKa = 8.95 SQEE482 pKa = 4.39 SIEE485 pKa = 4.32 SWQQSLMLASSQSALTKK502 pKa = 10.21 IITSKK507 pKa = 11.04 FIGKK511 pKa = 9.79 DD512 pKa = 2.68 IDD514 pKa = 3.92 SQGSSIHH521 pKa = 6.11 TYY523 pKa = 9.68 IDD525 pKa = 3.56 EE526 pKa = 6.07 DD527 pKa = 3.76 IMAHH531 pKa = 5.21 ITTNFFVKK539 pKa = 10.26 AWGVFSNFGQIMSGLLGFIIIGKK562 pKa = 8.69 LFKK565 pKa = 10.81 LVVDD569 pKa = 4.96 SIMNGIALYY578 pKa = 9.88 RR579 pKa = 11.84 VYY581 pKa = 10.78 GFSIALMGCIWDD593 pKa = 4.03 NVAHH597 pKa = 6.62 LLISFRR603 pKa = 11.84 KK604 pKa = 9.19 KK605 pKa = 10.31 DD606 pKa = 3.76 KK607 pKa = 11.17 KK608 pKa = 9.1 PTAPSKK614 pKa = 11.13 EE615 pKa = 4.21 DD616 pKa = 3.33 LCDD619 pKa = 3.81 NQCEE623 pKa = 4.21 PPAAQNPLLTQSTPVSMYY641 pKa = 10.09 PNVRR645 pKa = 11.84 SMTSHH650 pKa = 7.0 EE651 pKa = 4.14 LQQ653 pKa = 3.72
Molecular weight: 73.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.16
IPC2_protein 6.287
IPC_protein 6.364
Toseland 6.491
ProMoST 6.751
Dawson 6.766
Bjellqvist 6.766
Wikipedia 6.766
Rodwell 6.766
Grimsley 6.59
Solomon 6.766
Lehninger 6.766
Nozaki 7.059
DTASelect 7.161
Thurlkill 7.205
EMBOSS 7.176
Sillero 7.176
Patrickios 3.605
IPC_peptide 6.781
IPC2_peptide 6.912
IPC2.peptide.svr19 6.842
Protein with the highest isoelectric point:
>tr|A0A1L3KMU3|A0A1L3KMU3_9VIRU Uncharacterized protein OS=Hubei odonate virus 11 OX=1922992 PE=4 SV=1
MM1 pKa = 7.31 QNPAMEE7 pKa = 4.46 VDD9 pKa = 3.68 NVRR12 pKa = 11.84 PGPSGAQRR20 pKa = 11.84 PVVPEE25 pKa = 3.87 HH26 pKa = 6.59 QILTLGVRR34 pKa = 11.84 EE35 pKa = 3.97 PRR37 pKa = 11.84 YY38 pKa = 10.68 AEE40 pKa = 3.71 IAALAGKK47 pKa = 8.72 PDD49 pKa = 3.32 SGFRR53 pKa = 11.84 TSPSFSQQLFHH64 pKa = 7.54 NLVTPNIMYY73 pKa = 10.44 RR74 pKa = 11.84 GFLLTHH80 pKa = 6.56 TSANFPRR87 pKa = 11.84 VMYY90 pKa = 8.55 QTGAYY95 pKa = 9.48 LAGAVVAIGFAKK107 pKa = 10.02 MKK109 pKa = 9.7 VPQSATVDD117 pKa = 4.1 DD118 pKa = 3.96 IKK120 pKa = 11.23 LRR122 pKa = 11.84 IIGEE126 pKa = 4.07 VTAINIAPEE135 pKa = 4.36 LLLQTQEE142 pKa = 3.68 ADD144 pKa = 3.47 ALFLEE149 pKa = 4.44 IMNFILRR156 pKa = 11.84 SDD158 pKa = 3.56 PAIFRR163 pKa = 11.84 GGLEE167 pKa = 3.97 YY168 pKa = 10.58 TSDD171 pKa = 4.02 EE172 pKa = 4.34 FVSAYY177 pKa = 10.43 SDD179 pKa = 3.42 PRR181 pKa = 11.84 NVDD184 pKa = 3.25 VYY186 pKa = 10.97 PIGAGFKK193 pKa = 10.34 IPLPWSNDD201 pKa = 2.7 VEE203 pKa = 4.29 LRR205 pKa = 11.84 QNSMADD211 pKa = 3.2 ATRR214 pKa = 11.84 ICLWLTTKK222 pKa = 10.6 LANNYY227 pKa = 8.93 KK228 pKa = 9.42 NCKK231 pKa = 8.97 PEE233 pKa = 4.24 LSFSYY238 pKa = 10.61 LIAICRR244 pKa = 11.84 RR245 pKa = 11.84 GSVTTQFLTKK255 pKa = 10.46 VSDD258 pKa = 5.05 DD259 pKa = 3.42 INTEE263 pKa = 3.92 LGRR266 pKa = 11.84 TVSFDD271 pKa = 3.18 EE272 pKa = 4.09 DD273 pKa = 4.78 LIRR276 pKa = 11.84 KK277 pKa = 9.3 IYY279 pKa = 9.91 RR280 pKa = 11.84 IYY282 pKa = 11.03 GIFINEE288 pKa = 3.68 NNAEE292 pKa = 4.07 NVLRR296 pKa = 11.84 NILTWIPEE304 pKa = 3.71 EE305 pKa = 4.13 GLRR308 pKa = 11.84 LRR310 pKa = 11.84 LMLQHH315 pKa = 7.02 AEE317 pKa = 4.22 GSGLTPHH324 pKa = 5.73 YY325 pKa = 8.92 TVKK328 pKa = 10.48 EE329 pKa = 3.99 ALLKK333 pKa = 11.19 YY334 pKa = 10.19 PDD336 pKa = 4.34 FPWGRR341 pKa = 11.84 VDD343 pKa = 4.25 GLLSGEE349 pKa = 3.6 IRR351 pKa = 11.84 SFIRR355 pKa = 11.84 AWALIKK361 pKa = 11.01 GNLYY365 pKa = 10.84 YY366 pKa = 10.83 GFKK369 pKa = 10.09 RR370 pKa = 11.84 DD371 pKa = 3.54 LKK373 pKa = 9.54 EE374 pKa = 4.59 ASSQSFKK381 pKa = 11.12 GLAYY385 pKa = 9.89 VAKK388 pKa = 10.01 EE389 pKa = 4.06 LLIKK393 pKa = 10.59 AAGKK397 pKa = 10.59 GGLKK401 pKa = 10.08 AYY403 pKa = 10.11 RR404 pKa = 11.84 GWTRR408 pKa = 11.84 TPPSKK413 pKa = 10.9 GLLDD417 pKa = 4.68 EE418 pKa = 5.67 IIEE421 pKa = 4.42 NYY423 pKa = 8.62 LASMGEE429 pKa = 3.73 EE430 pKa = 3.8 AAALDD435 pKa = 4.1 QTTLDD440 pKa = 3.41 QLRR443 pKa = 11.84 FIANSTRR450 pKa = 3.22
Molecular weight: 50.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.046
IPC2_protein 7.439
IPC_protein 7.527
Toseland 7.366
ProMoST 8.141
Dawson 8.156
Bjellqvist 8.229
Wikipedia 8.126
Rodwell 8.156
Grimsley 7.38
Solomon 8.317
Lehninger 8.317
Nozaki 8.287
DTASelect 8.17
Thurlkill 8.214
EMBOSS 8.375
Sillero 8.448
Patrickios 4.393
IPC_peptide 8.317
IPC2_peptide 7.249
IPC2.peptide.svr19 7.564
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3271
450
2168
1090.3
124.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.044 ± 1.021
2.66 ± 0.574
4.983 ± 0.246
5.319 ± 0.191
4.127 ± 0.169
4.953 ± 0.627
2.293 ± 0.302
7.49 ± 0.372
6.023 ± 0.436
10.639 ± 0.887
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.14 ± 0.055
5.105 ± 0.056
3.944 ± 0.352
3.76 ± 0.141
4.769 ± 0.45
8.224 ± 0.755
6.084 ± 0.222
6.389 ± 0.364
1.192 ± 0.046
4.861 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here