Brevifollis gellanilyticus

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; Brevifollis

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5783 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512MDV2|A0A512MDV2_9BACT Non-specific serine/threonine protein kinase OS=Brevifollis gellanilyticus OX=748831 GN=BGE01nite_41630 PE=4 SV=1
MM1 pKa = 7.57IFLIFSGAHH10 pKa = 5.33FAWGYY15 pKa = 10.39LGSFEE20 pKa = 5.54EE21 pKa = 4.23NDD23 pKa = 4.12GYY25 pKa = 10.94RR26 pKa = 11.84VPSSGNILSLNFAGDD41 pKa = 3.39AQFYY45 pKa = 10.53LGNNPTNGLTGIVAPGAYY63 pKa = 9.27PSTLGDD69 pKa = 3.51ATHH72 pKa = 6.72GLDD75 pKa = 3.45VSRR78 pKa = 11.84YY79 pKa = 8.68NAGQYY84 pKa = 10.58GGGGGGPGGNGTDD97 pKa = 3.35IQDD100 pKa = 3.55NTGLWRR106 pKa = 11.84ALSGGRR112 pKa = 11.84LNEE115 pKa = 4.33DD116 pKa = 2.46QGAPYY121 pKa = 10.47YY122 pKa = 10.8LGGPFQRR129 pKa = 11.84DD130 pKa = 3.41YY131 pKa = 11.28IAGYY135 pKa = 10.12NYY137 pKa = 10.39GSARR141 pKa = 11.84TGSQVLNLLAYY152 pKa = 10.11DD153 pKa = 3.55EE154 pKa = 4.34NLRR157 pKa = 11.84YY158 pKa = 9.9SYY160 pKa = 11.45SLDD163 pKa = 3.29SRR165 pKa = 11.84DD166 pKa = 3.4FDD168 pKa = 4.87GINPATTSDD177 pKa = 3.37MRR179 pKa = 11.84IEE181 pKa = 4.1ASFWTCPTDD190 pKa = 3.94ADD192 pKa = 4.01DD193 pKa = 6.66DD194 pKa = 3.9IAIGVNTVGLSFRR207 pKa = 11.84DD208 pKa = 3.4SMDD211 pKa = 3.39QILVDD216 pKa = 4.05IGYY219 pKa = 8.65TGDD222 pKa = 3.7NFLQYY227 pKa = 10.45RR228 pKa = 11.84IGGSSTWQVTALNLGATGWSQINLVLDD255 pKa = 3.92TQANSVSLAARR266 pKa = 11.84AYY268 pKa = 10.83SDD270 pKa = 2.94ITGTLGTDD278 pKa = 3.14TTVFTDD284 pKa = 3.21QLLGFNTDD292 pKa = 2.61NVTQIEE298 pKa = 4.34WTATDD303 pKa = 2.95VDD305 pKa = 3.94GFKK308 pKa = 11.22NFFDD312 pKa = 5.4DD313 pKa = 5.3FDD315 pKa = 4.01FTLMSVVPEE324 pKa = 4.34PGSLTFLVLAWLYY337 pKa = 10.1SMRR340 pKa = 11.84RR341 pKa = 11.84KK342 pKa = 10.11RR343 pKa = 11.84EE344 pKa = 3.82SS345 pKa = 2.92

Molecular weight: 37.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512MIS1|A0A512MIS1_9BACT Uncharacterized protein OS=Brevifollis gellanilyticus OX=748831 GN=BGE01nite_54920 PE=4 SV=1
MM1 pKa = 7.1VAGAQQVRR9 pKa = 11.84RR10 pKa = 11.84ARR12 pKa = 11.84EE13 pKa = 3.62AAGLRR18 pKa = 11.84THH20 pKa = 7.14CAAKK24 pKa = 9.24STLPQLLQAMIQQALAGSGSLGHH47 pKa = 6.69HH48 pKa = 6.57LRR50 pKa = 11.84LVMNTRR56 pKa = 11.84ISDD59 pKa = 3.45SAAIQRR65 pKa = 11.84RR66 pKa = 11.84ASLNWEE72 pKa = 4.97FYY74 pKa = 9.96QEE76 pKa = 3.94LFGRR80 pKa = 11.84VLKK83 pKa = 10.5PLARR87 pKa = 11.84PKK89 pKa = 10.58DD90 pKa = 3.64HH91 pKa = 7.4AEE93 pKa = 3.94SFHH96 pKa = 7.28AGLRR100 pKa = 11.84LLALDD105 pKa = 4.77GSHH108 pKa = 7.52FSLRR112 pKa = 11.84NSAAVMAMTRR122 pKa = 11.84PRR124 pKa = 11.84PGNQRR129 pKa = 11.84ASAGAFMKK137 pKa = 10.31WSTAVLLEE145 pKa = 4.77LGTHH149 pKa = 6.62APLSVACSTLGMEE162 pKa = 4.41RR163 pKa = 11.84MEE165 pKa = 4.25MEE167 pKa = 3.39IDD169 pKa = 2.85IARR172 pKa = 11.84RR173 pKa = 11.84TLPTLATLGPSLLLADD189 pKa = 4.7RR190 pKa = 11.84LYY192 pKa = 11.29GCASFMLEE200 pKa = 4.85VMEE203 pKa = 4.14QGQGHH208 pKa = 6.33AHH210 pKa = 6.42CLMRR214 pKa = 11.84VRR216 pKa = 11.84DD217 pKa = 4.03NLRR220 pKa = 11.84AQQLEE225 pKa = 4.21VLHH228 pKa = 6.96DD229 pKa = 4.31GSALMRR235 pKa = 11.84VRR237 pKa = 11.84SDD239 pKa = 2.85TKK241 pKa = 10.99ARR243 pKa = 11.84AKK245 pKa = 10.29AASCEE250 pKa = 4.09MVVRR254 pKa = 11.84EE255 pKa = 4.13LRR257 pKa = 11.84AEE259 pKa = 3.97VRR261 pKa = 11.84RR262 pKa = 11.84TGGKK266 pKa = 9.59AQTLRR271 pKa = 11.84LWTTLLDD278 pKa = 4.0ARR280 pKa = 11.84KK281 pKa = 9.38HH282 pKa = 4.98PAEE285 pKa = 4.44EE286 pKa = 3.97LLKK289 pKa = 10.79LYY291 pKa = 9.91AQRR294 pKa = 11.84WEE296 pKa = 3.83QEE298 pKa = 3.86LFFRR302 pKa = 11.84EE303 pKa = 4.55LKK305 pKa = 10.12RR306 pKa = 11.84HH307 pKa = 5.34TGATSMLQADD317 pKa = 4.55SEE319 pKa = 4.47QTAQGAFAALVLAASEE335 pKa = 4.32VAQRR339 pKa = 11.84RR340 pKa = 11.84VEE342 pKa = 4.05AATEE346 pKa = 3.72ADD348 pKa = 4.08LPVLRR353 pKa = 11.84LSIAKK358 pKa = 8.51MGQMLGQLTSMLQSAGDD375 pKa = 3.82VLTPAQRR382 pKa = 11.84AAMSHH387 pKa = 5.79KK388 pKa = 8.64WLQVMVRR395 pKa = 11.84EE396 pKa = 4.18AVIPPRR402 pKa = 11.84RR403 pKa = 11.84ARR405 pKa = 11.84SCQHH409 pKa = 5.54GLRR412 pKa = 11.84RR413 pKa = 11.84PHH415 pKa = 6.64SPWPVIRR422 pKa = 11.84KK423 pKa = 8.89RR424 pKa = 11.84VFIDD428 pKa = 3.47PEE430 pKa = 4.14VLLTLLPATSPP441 pKa = 3.52

Molecular weight: 48.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5783

0

5783

2267602

39

12113

392.1

42.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.309 ± 0.038

0.927 ± 0.014

5.427 ± 0.031

5.722 ± 0.055

3.77 ± 0.018

8.326 ± 0.069

2.296 ± 0.027

4.758 ± 0.023

4.6 ± 0.056

9.865 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.022

3.331 ± 0.058

5.471 ± 0.031

3.62 ± 0.025

5.717 ± 0.042

6.243 ± 0.042

6.343 ± 0.094

6.94 ± 0.029

1.602 ± 0.019

2.387 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski