Nanoarchaeum equitans (strain Kin4-M)

Taxonomy: cellular organisms; Archaea; DPANN group; Nanoarchaeota; Nanoarchaeales; Nanoarchaeaceae; Nanoarchaeum; Nanoarchaeum equitans

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q74MM7|Q74MM7_NANEQ NEQ162 OS=Nanoarchaeum equitans (strain Kin4-M) OX=228908 GN=NEQ162 PE=3 SV=1
MM1 pKa = 8.43DD2 pKa = 5.09ILDD5 pKa = 3.95KK6 pKa = 11.16KK7 pKa = 10.54KK8 pKa = 10.67LPEE11 pKa = 4.09EE12 pKa = 4.35EE13 pKa = 4.47EE14 pKa = 4.02EE15 pKa = 4.14EE16 pKa = 4.66EE17 pKa = 3.97YY18 pKa = 11.16DD19 pKa = 4.12EE20 pKa = 5.42EE21 pKa = 4.78YY22 pKa = 10.94EE23 pKa = 4.57EE24 pKa = 4.43YY25 pKa = 10.16MEE27 pKa = 5.32DD28 pKa = 3.33LYY30 pKa = 11.64EE31 pKa = 4.19EE32 pKa = 4.45VEE34 pKa = 4.23EE35 pKa = 5.09EE36 pKa = 3.9EE37 pKa = 3.85DD38 pKa = 4.0DD39 pKa = 4.25YY40 pKa = 12.03YY41 pKa = 10.53IDD43 pKa = 4.06EE44 pKa = 4.45EE45 pKa = 4.45FEE47 pKa = 3.92EE48 pKa = 4.74LEE50 pKa = 4.41EE51 pKa = 4.84IEE53 pKa = 4.7EE54 pKa = 4.28EE55 pKa = 4.24

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q74MP0|Q74MP0_NANEQ NEQ180 OS=Nanoarchaeum equitans (strain Kin4-M) OX=228908 GN=NEQ180 PE=4 SV=1
MM1 pKa = 7.64GLYY4 pKa = 9.95QYY6 pKa = 10.98VRR8 pKa = 11.84ALYY11 pKa = 10.06KK12 pKa = 10.54DD13 pKa = 3.36PKK15 pKa = 9.97EE16 pKa = 4.14FLGEE20 pKa = 4.09LYY22 pKa = 10.15KK23 pKa = 10.66QRR25 pKa = 11.84IQQWRR30 pKa = 11.84RR31 pKa = 11.84EE32 pKa = 4.06PPIVEE37 pKa = 3.99VEE39 pKa = 3.86RR40 pKa = 11.84PTRR43 pKa = 11.84IDD45 pKa = 3.02RR46 pKa = 11.84ARR48 pKa = 11.84ALGYY52 pKa = 10.45KK53 pKa = 9.01PLPGIKK59 pKa = 8.84IVRR62 pKa = 11.84VRR64 pKa = 11.84VRR66 pKa = 11.84KK67 pKa = 7.96GTRR70 pKa = 11.84KK71 pKa = 9.68RR72 pKa = 11.84EE73 pKa = 4.0EE74 pKa = 3.82IKK76 pKa = 10.64GGRR79 pKa = 11.84RR80 pKa = 11.84PKK82 pKa = 10.15AEE84 pKa = 3.55YY85 pKa = 10.02RR86 pKa = 11.84IRR88 pKa = 11.84PLGVNLQWIAEE99 pKa = 3.96EE100 pKa = 4.04RR101 pKa = 11.84ANRR104 pKa = 11.84LHH106 pKa = 7.37RR107 pKa = 11.84NMEE110 pKa = 4.07VLGSYY115 pKa = 8.27WVGEE119 pKa = 3.99DD120 pKa = 5.3GLYY123 pKa = 10.17KK124 pKa = 9.53WYY126 pKa = 10.31EE127 pKa = 4.15VILVDD132 pKa = 4.54PFNPNIYY139 pKa = 10.15NRR141 pKa = 11.84PEE143 pKa = 4.14YY144 pKa = 10.95VWLLQKK150 pKa = 9.87NQRR153 pKa = 11.84GRR155 pKa = 11.84VFRR158 pKa = 11.84GKK160 pKa = 8.93TSSARR165 pKa = 11.84KK166 pKa = 9.12FRR168 pKa = 11.84GLRR171 pKa = 11.84NGGLGAEE178 pKa = 4.52KK179 pKa = 10.49VRR181 pKa = 11.84PSKK184 pKa = 10.57RR185 pKa = 11.84ANFKK189 pKa = 10.62DD190 pKa = 3.34

Molecular weight: 22.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

536

0

536

151454

54

2197

282.6

32.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.186 ± 0.081

0.81 ± 0.058

4.972 ± 0.07

7.88 ± 0.134

4.434 ± 0.088

5.303 ± 0.077

1.343 ± 0.037

10.456 ± 0.124

10.826 ± 0.112

10.422 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.703 ± 0.044

5.262 ± 0.123

4.005 ± 0.058

2.216 ± 0.046

3.901 ± 0.092

4.622 ± 0.097

4.106 ± 0.084

5.942 ± 0.098

0.963 ± 0.036

5.646 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski