Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis; Sphingopyxis alaskensis

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1GQW0|Q1GQW0_SPHAL Ferrochelatase OS=Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) OX=317655 GN=hemH PE=3 SV=1
MM1 pKa = 7.0QSVRR5 pKa = 11.84ACIMLVWLALTFASNPVTAQTTTSYY30 pKa = 10.02STTTTGTISEE40 pKa = 4.55SATPCSAPMLRR51 pKa = 11.84SFTVAANAQVSDD63 pKa = 3.95VNIGVQFAHH72 pKa = 7.09TYY74 pKa = 10.21RR75 pKa = 11.84GDD77 pKa = 3.07IRR79 pKa = 11.84ATLTSPAGTVVDD91 pKa = 5.93LITNVGASADD101 pKa = 3.81NLNVLFDD108 pKa = 4.82DD109 pKa = 5.24GAAASITTHH118 pKa = 6.27NANDD122 pKa = 3.77STATAPPYY130 pKa = 10.48QRR132 pKa = 11.84TFAPQGSLAAFNGQASGGTWQLSICDD158 pKa = 4.45SLNGDD163 pKa = 3.33AGTFTRR169 pKa = 11.84ADD171 pKa = 3.55LTLTTVPLSPYY182 pKa = 10.78ADD184 pKa = 3.59LSLAKK189 pKa = 8.98TVSNAAPASGAIINYY204 pKa = 8.45VLSVTNAGASTLTATGVTVQDD225 pKa = 3.59TLPAGFAFTGASGFGSYY242 pKa = 10.96NSTTGVWTVGSVPPGVTRR260 pKa = 11.84TLTISGTVAASAGASVTNSAEE281 pKa = 3.92IAASSAFDD289 pKa = 4.13PDD291 pKa = 3.72STPGNGAAGEE301 pKa = 4.32DD302 pKa = 4.36DD303 pKa = 4.18GDD305 pKa = 4.04SASFTVSGTRR315 pKa = 11.84VAGTPPTLVCPVGTTALDD333 pKa = 3.36WDD335 pKa = 4.71TIAWNAGTTSASPPLTAIGTASIGIAVSGGVFLSNATYY373 pKa = 10.51GGQSPTRR380 pKa = 11.84QNIVTGGLSPAQYY393 pKa = 10.79SIFQLVDD400 pKa = 3.78FTSQAGSVATTISLPTAVPGAQFRR424 pKa = 11.84LFDD427 pKa = 3.33VDD429 pKa = 3.59YY430 pKa = 11.14AAGQFADD437 pKa = 3.61RR438 pKa = 11.84VTVTGSYY445 pKa = 10.45NGAPVTPILTNGTANYY461 pKa = 10.26VIGNSAYY468 pKa = 10.55GDD470 pKa = 3.84VLSTDD475 pKa = 3.08ASGNGNITVTFAAPVDD491 pKa = 4.25TIVIDD496 pKa = 4.04YY497 pKa = 9.9GSHH500 pKa = 6.53ALAPANPGQQGMALHH515 pKa = 7.36DD516 pKa = 4.74IIFCRR521 pKa = 11.84PTANLTVAKK530 pKa = 9.05TSSIVSDD537 pKa = 3.73PVGGATNPKK546 pKa = 9.23AIPGATMRR554 pKa = 11.84YY555 pKa = 9.18CILVTNNGSGTATAINIADD574 pKa = 4.4ALPARR579 pKa = 11.84TTFVPGSLRR588 pKa = 11.84SGTSCAAATTVEE600 pKa = 4.72DD601 pKa = 4.78DD602 pKa = 3.68NASGGDD608 pKa = 3.38EE609 pKa = 4.31SDD611 pKa = 3.53PFGASFAGSAIAASTATLAPGGAMAIAFTVTIDD644 pKa = 3.0

Molecular weight: 64.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1GQ97|Q1GQ97_SPHAL Oxidoreductase FAD-binding region OS=Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) OX=317655 GN=Sala_2468 PE=4 SV=1
MM1 pKa = 7.52SSPRR5 pKa = 11.84GSDD8 pKa = 3.35PASGAGDD15 pKa = 3.17WSGNWRR21 pKa = 11.84TLVGLWAIPGLAMLAAMWLEE41 pKa = 3.91PTPRR45 pKa = 11.84AVIWTVMLASMGAACIMNARR65 pKa = 11.84RR66 pKa = 11.84CGRR69 pKa = 11.84THH71 pKa = 6.99CRR73 pKa = 11.84FTGPFLIGMAILVVAYY89 pKa = 9.92AIGMLPLGPHH99 pKa = 5.86GWGILGGVTLGGFAALWWGSEE120 pKa = 4.11RR121 pKa = 11.84AWGKK125 pKa = 9.76FSRR128 pKa = 11.84SKK130 pKa = 11.18DD131 pKa = 3.26KK132 pKa = 11.16GVSRR136 pKa = 11.84SCC138 pKa = 3.95

Molecular weight: 14.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3184

0

3184

1011156

41

2090

317.6

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.039 ± 0.071

0.805 ± 0.015

6.197 ± 0.035

5.336 ± 0.04

3.529 ± 0.026

8.921 ± 0.045

2.015 ± 0.02

5.113 ± 0.031

2.912 ± 0.036

9.765 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.019

2.391 ± 0.025

5.383 ± 0.035

2.877 ± 0.021

7.649 ± 0.046

4.969 ± 0.031

5.021 ± 0.027

7.021 ± 0.035

1.462 ± 0.018

2.111 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski