Amino acid dipepetide frequency for Paenibacillus nuruki

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.096AlaAla: 6.096 ± 0.088	0.637AlaCys: 0.637 ± 0.021	4.221AlaAsp: 4.221 ± 0.06	4.96AlaGlu: 4.96 ± 0.072	2.977AlaPhe: 2.977 ± 0.049	5.832AlaGly: 5.832 ± 0.077	1.56AlaHis: 1.56 ± 0.038	5.633AlaIle: 5.633 ± 0.066	3.988AlaLys: 3.988 ± 0.06	7.507AlaLeu: 7.507 ± 0.074	2.0AlaMet: 2.0 ± 0.037	2.87AlaAsn: 2.87 ± 0.051	2.516AlaPro: 2.516 ± 0.044	3.356AlaGln: 3.356 ± 0.051	2.926AlaArg: 2.926 ± 0.044	5.045AlaSer: 5.045 ± 0.068	4.586AlaThr: 4.586 ± 0.074	5.61AlaVal: 5.61 ± 0.077	0.742AlaTrp: 0.742 ± 0.025	2.663AlaTyr: 2.663 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.479CysAla: 0.479 ± 0.018	0.1CysCys: 0.1 ± 0.009	0.377CysAsp: 0.377 ± 0.017	0.396CysGlu: 0.396 ± 0.016	0.287CysPhe: 0.287 ± 0.013	0.625CysGly: 0.625 ± 0.02	0.182CysHis: 0.182 ± 0.012	0.625CysIle: 0.625 ± 0.022	0.277CysLys: 0.277 ± 0.014	0.659CysLeu: 0.659 ± 0.021	0.199CysMet: 0.199 ± 0.011	0.279CysAsn: 0.279 ± 0.014	0.314CysPro: 0.314 ± 0.015	0.241CysGln: 0.241 ± 0.013	0.352CysArg: 0.352 ± 0.016	0.48CysSer: 0.48 ± 0.021	0.415CysThr: 0.415 ± 0.018	0.406CysVal: 0.406 ± 0.017	0.079CysTrp: 0.079 ± 0.007	0.279CysTyr: 0.279 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.575AspAla: 3.575 ± 0.056	0.367AspCys: 0.367 ± 0.015	2.518AspAsp: 2.518 ± 0.047	3.545AspGlu: 3.545 ± 0.054	2.002AspPhe: 2.002 ± 0.043	3.485AspGly: 3.485 ± 0.052	1.599AspHis: 1.599 ± 0.034	4.297AspIle: 4.297 ± 0.05	2.5AspLys: 2.5 ± 0.045	5.003AspLeu: 5.003 ± 0.059	1.536AspMet: 1.536 ± 0.036	1.972AspAsn: 1.972 ± 0.04	2.413AspPro: 2.413 ± 0.04	2.952AspGln: 2.952 ± 0.054	2.511AspArg: 2.511 ± 0.039	2.979AspSer: 2.979 ± 0.052	3.055AspThr: 3.055 ± 0.047	3.389AspVal: 3.389 ± 0.057	0.818AspTrp: 0.818 ± 0.026	2.333AspTyr: 2.333 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.115GluAla: 5.115 ± 0.06	0.388GluCys: 0.388 ± 0.016	3.276GluAsp: 3.276 ± 0.057	4.976GluGlu: 4.976 ± 0.076	2.036GluPhe: 2.036 ± 0.039	3.766GluGly: 3.766 ± 0.052	1.898GluHis: 1.898 ± 0.034	4.376GluIle: 4.376 ± 0.063	3.834GluLys: 3.834 ± 0.057	6.674GluLeu: 6.674 ± 0.083	1.972GluMet: 1.972 ± 0.045	2.529GluAsn: 2.529 ± 0.04	2.062GluPro: 2.062 ± 0.041	4.711GluGln: 4.711 ± 0.084	3.332GluArg: 3.332 ± 0.052	3.708GluSer: 3.708 ± 0.055	2.866GluThr: 2.866 ± 0.051	4.42GluVal: 4.42 ± 0.062	0.935GluTrp: 0.935 ± 0.025	2.291GluTyr: 2.291 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	3.17PheAla: 3.17 ± 0.049	0.338PheCys: 0.338 ± 0.016	2.164PheAsp: 2.164 ± 0.035	2.355PheGlu: 2.355 ± 0.041	1.719PhePhe: 1.719 ± 0.043	2.829PheGly: 2.829 ± 0.051	0.82PheHis: 0.82 ± 0.024	2.93PheIle: 2.93 ± 0.041	1.964PheLys: 1.964 ± 0.042	3.504PheLeu: 3.504 ± 0.058	1.051PheMet: 1.051 ± 0.025	1.66PheAsn: 1.66 ± 0.038	1.498PhePro: 1.498 ± 0.031	1.506PheGln: 1.506 ± 0.031	1.64PheArg: 1.64 ± 0.032	2.593PheSer: 2.593 ± 0.044	2.445PheThr: 2.445 ± 0.047	2.815PheVal: 2.815 ± 0.049	0.533PheTrp: 0.533 ± 0.017	1.495PheTyr: 1.495 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	4.84GlyAla: 4.84 ± 0.067	0.567GlyCys: 0.567 ± 0.02	3.244GlyAsp: 3.244 ± 0.052	3.974GlyGlu: 3.974 ± 0.061	2.87GlyPhe: 2.87 ± 0.049	4.734GlyGly: 4.734 ± 0.076	1.421GlyHis: 1.421 ± 0.028	5.737GlyIle: 5.737 ± 0.067	4.117GlyLys: 4.117 ± 0.055	6.395GlyLeu: 6.395 ± 0.08	2.135GlyMet: 2.135 ± 0.038	2.666GlyAsn: 2.666 ± 0.05	1.43GlyPro: 1.43 ± 0.034	2.885GlyGln: 2.885 ± 0.046	2.704GlyArg: 2.704 ± 0.045	4.355GlySer: 4.355 ± 0.07	4.448GlyThr: 4.448 ± 0.074	4.802GlyVal: 4.802 ± 0.063	0.948GlyTrp: 0.948 ± 0.029	2.811GlyTyr: 2.811 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	1.582HisAla: 1.582 ± 0.035	0.206HisCys: 0.206 ± 0.013	1.1HisAsp: 1.1 ± 0.027	1.404HisGlu: 1.404 ± 0.035	1.091HisPhe: 1.091 ± 0.027	1.437HisGly: 1.437 ± 0.033	0.812HisHis: 0.812 ± 0.027	1.962HisIle: 1.962 ± 0.044	0.906HisLys: 0.906 ± 0.029	2.36HisLeu: 2.36 ± 0.044	0.71HisMet: 0.71 ± 0.022	0.843HisAsn: 0.843 ± 0.023	1.34HisPro: 1.34 ± 0.03	1.189HisGln: 1.189 ± 0.031	1.075HisArg: 1.075 ± 0.028	1.464HisSer: 1.464 ± 0.027	1.444HisThr: 1.444 ± 0.034	1.552HisVal: 1.552 ± 0.03	0.351HisTrp: 0.351 ± 0.015	1.066HisTyr: 1.066 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.439IleAla: 6.439 ± 0.09	0.642IleCys: 0.642 ± 0.02	4.247IleAsp: 4.247 ± 0.054	5.097IleGlu: 5.097 ± 0.069	2.693IlePhe: 2.693 ± 0.049	5.438IleGly: 5.438 ± 0.065	1.892IleHis: 1.892 ± 0.037	5.041IleIle: 5.041 ± 0.075	3.298IleLys: 3.298 ± 0.047	6.555IleLeu: 6.555 ± 0.093	1.816IleMet: 1.816 ± 0.037	2.929IleAsn: 2.929 ± 0.055	3.467IlePro: 3.467 ± 0.055	3.609IleGln: 3.609 ± 0.057	3.491IleArg: 3.491 ± 0.053	4.93IleSer: 4.93 ± 0.066	4.555IleThr: 4.555 ± 0.065	5.478IleVal: 5.478 ± 0.062	0.795IleTrp: 0.795 ± 0.022	2.369IleTyr: 2.369 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	3.812LysAla: 3.812 ± 0.066	0.249LysCys: 0.249 ± 0.013	2.949LysAsp: 2.949 ± 0.052	4.061LysGlu: 4.061 ± 0.061	1.461LysPhe: 1.461 ± 0.032	3.501LysGly: 3.501 ± 0.055	1.151LysHis: 1.151 ± 0.03	3.448LysIle: 3.448 ± 0.053	3.317LysLys: 3.317 ± 0.058	4.76LysLeu: 4.76 ± 0.054	1.717LysMet: 1.717 ± 0.039	2.338LysAsn: 2.338 ± 0.047	1.944LysPro: 1.944 ± 0.033	2.717LysGln: 2.717 ± 0.05	2.425LysArg: 2.425 ± 0.045	3.102LysSer: 3.102 ± 0.043	2.721LysThr: 2.721 ± 0.05	3.907LysVal: 3.907 ± 0.055	0.77LysTrp: 0.77 ± 0.021	1.891LysTyr: 1.891 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	7.421LeuAla: 7.421 ± 0.077	0.773LeuCys: 0.773 ± 0.02	5.107LeuAsp: 5.107 ± 0.07	6.081LeuGlu: 6.081 ± 0.076	4.157LeuPhe: 4.157 ± 0.06	6.099LeuGly: 6.099 ± 0.078	2.338LeuHis: 2.338 ± 0.046	7.08LeuIle: 7.08 ± 0.087	4.939LeuLys: 4.939 ± 0.056	10.153LeuLeu: 10.153 ± 0.117	2.532LeuMet: 2.532 ± 0.048	4.207LeuAsn: 4.207 ± 0.057	4.321LeuPro: 4.321 ± 0.065	4.706LeuGln: 4.706 ± 0.069	4.103LeuArg: 4.103 ± 0.061	7.148LeuSer: 7.148 ± 0.066	5.803LeuThr: 5.803 ± 0.06	5.739LeuVal: 5.739 ± 0.071	1.031LeuTrp: 1.031 ± 0.027	3.346LeuTyr: 3.346 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	1.967MetAla: 1.967 ± 0.036	0.158MetCys: 0.158 ± 0.01	1.501MetAsp: 1.501 ± 0.036	1.679MetGlu: 1.679 ± 0.032	0.928MetPhe: 0.928 ± 0.023	1.633MetGly: 1.633 ± 0.034	0.497MetHis: 0.497 ± 0.019	2.526MetIle: 2.526 ± 0.045	1.897MetLys: 1.897 ± 0.033	2.725MetLeu: 2.725 ± 0.045	0.992MetMet: 0.992 ± 0.03	1.776MetAsn: 1.776 ± 0.037	1.061MetPro: 1.061 ± 0.026	1.168MetGln: 1.168 ± 0.027	1.183MetArg: 1.183 ± 0.03	1.952MetSer: 1.952 ± 0.037	1.801MetThr: 1.801 ± 0.031	1.826MetVal: 1.826 ± 0.037	0.226MetTrp: 0.226 ± 0.013	0.886MetTyr: 0.886 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.823AsnAla: 2.823 ± 0.057	0.233AsnCys: 0.233 ± 0.013	2.19AsnAsp: 2.19 ± 0.039	2.754AsnGlu: 2.754 ± 0.047	1.393AsnPhe: 1.393 ± 0.034	3.096AsnGly: 3.096 ± 0.06	1.115AsnHis: 1.115 ± 0.027	3.094AsnIle: 3.094 ± 0.051	2.264AsnLys: 2.264 ± 0.044	3.541AsnLeu: 3.541 ± 0.043	1.218AsnMet: 1.218 ± 0.028	2.093AsnAsn: 2.093 ± 0.045	2.09AsnPro: 2.09 ± 0.039	2.077AsnGln: 2.077 ± 0.036	1.938AsnArg: 1.938 ± 0.034	2.489AsnSer: 2.489 ± 0.052	2.668AsnThr: 2.668 ± 0.051	2.83AsnVal: 2.83 ± 0.049	0.568AsnTrp: 0.568 ± 0.021	1.535AsnTyr: 1.535 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	3.161ProAla: 3.161 ± 0.05	0.198ProCys: 0.198 ± 0.012	2.461ProAsp: 2.461 ± 0.047	3.141ProGlu: 3.141 ± 0.05	1.738ProPhe: 1.738 ± 0.031	2.192ProGly: 2.192 ± 0.042	0.894ProHis: 0.894 ± 0.027	2.73ProIle: 2.73 ± 0.04	1.753ProLys: 1.753 ± 0.033	3.838ProLeu: 3.838 ± 0.058	0.921ProMet: 0.921 ± 0.026	1.676ProAsn: 1.676 ± 0.036	1.074ProPro: 1.074 ± 0.035	1.629ProGln: 1.629 ± 0.033	1.173ProArg: 1.173 ± 0.031	2.605ProSer: 2.605 ± 0.047	2.487ProThr: 2.487 ± 0.049	3.077ProVal: 3.077 ± 0.052	0.445ProTrp: 0.445 ± 0.019	1.521ProTyr: 1.521 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.126GlnAla: 4.126 ± 0.058	0.253GlnCys: 0.253 ± 0.012	2.161GlnAsp: 2.161 ± 0.035	3.088GlnGlu: 3.088 ± 0.061	1.725GlnPhe: 1.725 ± 0.036	2.825GlnGly: 2.825 ± 0.047	1.329GlnHis: 1.329 ± 0.037	3.255GlnIle: 3.255 ± 0.052	2.473GlnLys: 2.473 ± 0.044	5.201GlnLeu: 5.201 ± 0.08	1.482GlnMet: 1.482 ± 0.029	1.753GlnAsn: 1.753 ± 0.034	1.864GlnPro: 1.864 ± 0.035	3.332GlnGln: 3.332 ± 0.084	2.05GlnArg: 2.05 ± 0.044	3.269GlnSer: 3.269 ± 0.052	2.648GlnThr: 2.648 ± 0.044	3.069GlnVal: 3.069 ± 0.044	0.696GlnTrp: 0.696 ± 0.024	1.879GlnTyr: 1.879 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.779ArgAla: 2.779 ± 0.049	0.266ArgCys: 0.266 ± 0.014	2.123ArgAsp: 2.123 ± 0.035	2.984ArgGlu: 2.984 ± 0.05	1.746ArgPhe: 1.746 ± 0.034	2.371ArgGly: 2.371 ± 0.043	0.976ArgHis: 0.976 ± 0.025	3.411ArgIle: 3.411 ± 0.051	2.664ArgLys: 2.664 ± 0.046	4.525ArgLeu: 4.525 ± 0.067	1.364ArgMet: 1.364 ± 0.031	1.81ArgAsn: 1.81 ± 0.035	1.496ArgPro: 1.496 ± 0.031	2.174ArgGln: 2.174 ± 0.037	2.081ArgArg: 2.081 ± 0.045	2.767ArgSer: 2.767 ± 0.046	2.504ArgThr: 2.504 ± 0.039	2.553ArgVal: 2.553 ± 0.037	0.574ArgTrp: 0.574 ± 0.02	1.9ArgTyr: 1.9 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	4.766SerAla: 4.766 ± 0.063	0.403SerCys: 0.403 ± 0.018	3.299SerAsp: 3.299 ± 0.05	3.756SerGlu: 3.756 ± 0.05	2.91SerPhe: 2.91 ± 0.043	4.867SerGly: 4.867 ± 0.071	1.408SerHis: 1.408 ± 0.032	5.141SerIle: 5.141 ± 0.064	3.449SerLys: 3.449 ± 0.057	6.445SerLeu: 6.445 ± 0.07	1.906SerMet: 1.906 ± 0.037	2.859SerAsn: 2.859 ± 0.053	2.368SerPro: 2.368 ± 0.046	2.618SerGln: 2.618 ± 0.042	2.606SerArg: 2.606 ± 0.045	5.358SerSer: 5.358 ± 0.08	4.338SerThr: 4.338 ± 0.072	4.366SerVal: 4.366 ± 0.054	0.858SerTrp: 0.858 ± 0.028	2.493SerTyr: 2.493 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	5.029ThrAla: 5.029 ± 0.07	0.335ThrCys: 0.335 ± 0.015	3.447ThrAsp: 3.447 ± 0.055	3.626ThrGlu: 3.626 ± 0.055	2.47ThrPhe: 2.47 ± 0.044	4.673ThrGly: 4.673 ± 0.063	1.306ThrHis: 1.306 ± 0.026	4.407ThrIle: 4.407 ± 0.059	2.644ThrLys: 2.644 ± 0.055	5.919ThrLeu: 5.919 ± 0.07	1.45ThrMet: 1.45 ± 0.03	2.362ThrAsn: 2.362 ± 0.044	2.901ThrPro: 2.901 ± 0.052	2.174ThrGln: 2.174 ± 0.047	2.179ThrArg: 2.179 ± 0.038	4.063ThrSer: 4.063 ± 0.066	4.116ThrThr: 4.116 ± 0.086	4.439ThrVal: 4.439 ± 0.064	0.699ThrTrp: 0.699 ± 0.021	2.182ThrTyr: 2.182 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	5.174ValAla: 5.174 ± 0.063	0.545ValCys: 0.545 ± 0.017	3.674ValAsp: 3.674 ± 0.053	4.221ValGlu: 4.221 ± 0.055	2.701ValPhe: 2.701 ± 0.041	4.351ValGly: 4.351 ± 0.064	1.535ValHis: 1.535 ± 0.031	5.293ValIle: 5.293 ± 0.066	3.505ValLys: 3.505 ± 0.047	6.649ValLeu: 6.649 ± 0.073	1.948ValMet: 1.948 ± 0.037	2.922ValAsn: 2.922 ± 0.051	2.723ValPro: 2.723 ± 0.045	3.094ValGln: 3.094 ± 0.043	2.774ValArg: 2.774 ± 0.044	4.639ValSer: 4.639 ± 0.049	4.439ValThr: 4.439 ± 0.066	4.711ValVal: 4.711 ± 0.057	0.801ValTrp: 0.801 ± 0.022	2.385ValTyr: 2.385 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.743TrpAla: 0.743 ± 0.025	0.09TrpCys: 0.09 ± 0.006	0.65TrpAsp: 0.65 ± 0.024	0.764TrpGlu: 0.764 ± 0.026	0.518TrpPhe: 0.518 ± 0.017	0.743TrpGly: 0.743 ± 0.023	0.271TrpHis: 0.271 ± 0.012	1.133TrpIle: 1.133 ± 0.031	0.753TrpLys: 0.753 ± 0.027	1.33TrpLeu: 1.33 ± 0.034	0.428TrpMet: 0.428 ± 0.018	0.743TrpAsn: 0.743 ± 0.024	0.252TrpPro: 0.252 ± 0.014	0.614TrpGln: 0.614 ± 0.024	0.52TrpArg: 0.52 ± 0.02	0.854TrpSer: 0.854 ± 0.023	0.685TrpThr: 0.685 ± 0.025	0.786TrpVal: 0.786 ± 0.026	0.175TrpTrp: 0.175 ± 0.011	0.43TrpTyr: 0.43 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.628TyrAla: 2.628 ± 0.045	0.302TyrCys: 0.302 ± 0.013	2.139TyrAsp: 2.139 ± 0.048	2.305TyrGlu: 2.305 ± 0.036	1.607TyrPhe: 1.607 ± 0.031	2.585TyrGly: 2.585 ± 0.04	0.891TyrHis: 0.891 ± 0.027	2.664TyrIle: 2.664 ± 0.042	1.699TyrLys: 1.699 ± 0.037	3.337TyrLeu: 3.337 ± 0.061	1.004TyrMet: 1.004 ± 0.028	1.725TyrAsn: 1.725 ± 0.036	1.58TyrPro: 1.58 ± 0.032	1.725TyrGln: 1.725 ± 0.034	2.007TyrArg: 2.007 ± 0.038	2.415TyrSer: 2.415 ± 0.047	2.399TyrThr: 2.399 ± 0.041	2.307TyrVal: 2.307 ± 0.034	0.437TyrTrp: 0.437 ± 0.019	1.494TyrTyr: 1.494 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4645 proteins (1523825 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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