Hypsibius dujardini (Water bear) (Macrobiotus dujardini)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Tardigrada; Eutardigrada; Parachela; Hypsibioidea; Hypsibiidae; Hypsibius

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14867 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W0WRC8|A0A1W0WRC8_HYPDU MOB kinase activator-like 4 OS=Hypsibius dujardini OX=232323 GN=BV898_08170 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 4.39SPEE5 pKa = 3.96NMTAFGAWSSVTFPPIDD22 pKa = 3.83YY23 pKa = 8.76PPSSSVVVTHH33 pKa = 6.71LEE35 pKa = 4.2PPAPPAIYY43 pKa = 10.7ALLQQLLVVIGSSLSLVFIAFAFITFTLFSDD74 pKa = 4.44LKK76 pKa = 11.06DD77 pKa = 3.49LAGG80 pKa = 3.88

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W0XAN4|A0A1W0XAN4_HYPDU cAMP-dependent protein kinase type II regulatory subunit OS=Hypsibius dujardini OX=232323 GN=BV898_01870 PE=4 SV=1
MM1 pKa = 7.19NHH3 pKa = 6.85PAADD7 pKa = 3.66NSIGGIRR14 pKa = 11.84RR15 pKa = 11.84SPHH18 pKa = 5.69SPHH21 pKa = 6.78ALGRR25 pKa = 11.84HH26 pKa = 5.33GKK28 pKa = 6.34PTRR31 pKa = 11.84SHH33 pKa = 6.34SPHH36 pKa = 7.29DD37 pKa = 3.67KK38 pKa = 10.45RR39 pKa = 11.84PTGVRR44 pKa = 11.84SPSEE48 pKa = 3.83VARR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84AQRR56 pKa = 11.84MKK58 pKa = 10.54SRR60 pKa = 11.84RR61 pKa = 11.84ASRR64 pKa = 11.84KK65 pKa = 5.21STRR68 pKa = 11.84RR69 pKa = 11.84SHH71 pKa = 6.51RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84SSRR77 pKa = 11.84SRR79 pKa = 11.84SVRR82 pKa = 11.84RR83 pKa = 11.84GRR85 pKa = 11.84SARR88 pKa = 11.84NRR90 pKa = 11.84TRR92 pKa = 11.84KK93 pKa = 9.45SRR95 pKa = 11.84RR96 pKa = 11.84SVKK99 pKa = 10.5KK100 pKa = 8.32IGKK103 pKa = 8.88RR104 pKa = 11.84RR105 pKa = 11.84AVSHH109 pKa = 6.13RR110 pKa = 11.84VVTKK114 pKa = 10.44KK115 pKa = 10.44RR116 pKa = 11.84GTRR119 pKa = 11.84RR120 pKa = 11.84LRR122 pKa = 11.84RR123 pKa = 11.84SGSGRR128 pKa = 11.84SHH130 pKa = 6.59RR131 pKa = 11.84RR132 pKa = 11.84SS133 pKa = 3.17

Molecular weight: 15.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14162

705

14867

6817457

19

9275

458.6

50.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.853 ± 0.017

1.86 ± 0.015

5.415 ± 0.017

5.682 ± 0.026

4.171 ± 0.015

6.571 ± 0.026

2.405 ± 0.011

4.884 ± 0.017

4.938 ± 0.023

9.07 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.009

4.069 ± 0.013

5.683 ± 0.024

4.127 ± 0.017

5.964 ± 0.017

8.254 ± 0.025

6.11 ± 0.024

6.686 ± 0.018

1.216 ± 0.007

2.765 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski