Amino acid dipepetide frequency for Hypsibius dujardini (Water bear) (Macrobiotus dujardini)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.531AlaAla: 8.531 ± 0.068	1.292AlaCys: 1.292 ± 0.017	4.164AlaAsp: 4.164 ± 0.025	4.579AlaGlu: 4.579 ± 0.032	3.112AlaPhe: 3.112 ± 0.025	5.258AlaGly: 5.258 ± 0.039	1.554AlaHis: 1.554 ± 0.018	3.697AlaIle: 3.697 ± 0.027	3.922AlaLys: 3.922 ± 0.035	6.776AlaLeu: 6.776 ± 0.035	1.772AlaMet: 1.772 ± 0.017	2.914AlaAsn: 2.914 ± 0.023	4.128AlaPro: 4.128 ± 0.045	2.749AlaGln: 2.749 ± 0.028	3.9AlaArg: 3.9 ± 0.029	6.273AlaSer: 6.273 ± 0.036	4.998AlaThr: 4.998 ± 0.044	6.253AlaVal: 6.253 ± 0.039	0.822AlaTrp: 0.822 ± 0.011	1.835AlaTyr: 1.835 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	1.22CysAla: 1.22 ± 0.016	0.533CysCys: 0.533 ± 0.012	1.067CysAsp: 1.067 ± 0.02	0.866CysGlu: 0.866 ± 0.016	0.831CysPhe: 0.831 ± 0.013	1.493CysGly: 1.493 ± 0.022	0.461CysHis: 0.461 ± 0.01	0.883CysIle: 0.883 ± 0.016	0.773CysLys: 0.773 ± 0.013	1.891CysLeu: 1.891 ± 0.022	0.369CysMet: 0.369 ± 0.008	0.703CysAsn: 0.703 ± 0.012	1.06CysPro: 1.06 ± 0.019	0.761CysGln: 0.761 ± 0.013	1.187CysArg: 1.187 ± 0.015	1.503CysSer: 1.503 ± 0.019	0.971CysThr: 0.971 ± 0.015	1.216CysVal: 1.216 ± 0.018	0.281CysTrp: 0.281 ± 0.006	0.535CysTyr: 0.535 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	4.045AspAla: 4.045 ± 0.032	1.015AspCys: 1.015 ± 0.018	3.69AspAsp: 3.69 ± 0.058	3.772AspGlu: 3.772 ± 0.048	2.467AspPhe: 2.467 ± 0.021	4.074AspGly: 4.074 ± 0.035	1.309AspHis: 1.309 ± 0.017	2.725AspIle: 2.725 ± 0.021	2.272AspLys: 2.272 ± 0.022	5.083AspLeu: 5.083 ± 0.031	1.155AspMet: 1.155 ± 0.014	1.924AspAsn: 1.924 ± 0.017	2.776AspPro: 2.776 ± 0.021	1.976AspGln: 1.976 ± 0.019	3.139AspArg: 3.139 ± 0.027	4.11AspSer: 4.11 ± 0.027	2.47AspThr: 2.47 ± 0.02	3.748AspVal: 3.748 ± 0.028	0.719AspTrp: 0.719 ± 0.012	1.676AspTyr: 1.676 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	4.276GluAla: 4.276 ± 0.037	0.873GluCys: 0.873 ± 0.018	3.66GluAsp: 3.66 ± 0.044	4.913GluGlu: 4.913 ± 0.062	2.101GluPhe: 2.101 ± 0.019	2.853GluGly: 2.853 ± 0.022	1.235GluHis: 1.235 ± 0.015	3.008GluIle: 3.008 ± 0.026	3.656GluLys: 3.656 ± 0.035	5.034GluLeu: 5.034 ± 0.039	1.527GluMet: 1.527 ± 0.016	2.445GluAsn: 2.445 ± 0.025	2.55GluPro: 2.55 ± 0.024	2.339GluGln: 2.339 ± 0.023	3.496GluArg: 3.496 ± 0.031	3.928GluSer: 3.928 ± 0.031	3.241GluThr: 3.241 ± 0.029	3.456GluVal: 3.456 ± 0.028	0.697GluTrp: 0.697 ± 0.011	1.535GluTyr: 1.535 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	3.084PheAla: 3.084 ± 0.025	0.944PheCys: 0.944 ± 0.013	2.274PheAsp: 2.274 ± 0.019	2.035PheGlu: 2.035 ± 0.019	1.958PhePhe: 1.958 ± 0.021	3.036PheGly: 3.036 ± 0.032	1.119PheHis: 1.119 ± 0.014	2.131PheIle: 2.131 ± 0.021	1.594PheLys: 1.594 ± 0.016	4.159PheLeu: 4.159 ± 0.036	0.909PheMet: 0.909 ± 0.013	1.685PheAsn: 1.685 ± 0.015	2.212PhePro: 2.212 ± 0.022	1.64PheGln: 1.64 ± 0.014	2.508PheArg: 2.508 ± 0.02	3.301PheSer: 3.301 ± 0.034	2.366PheThr: 2.366 ± 0.02	2.914PheVal: 2.914 ± 0.025	0.562PheTrp: 0.562 ± 0.011	1.277PheTyr: 1.277 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	4.382GlyAla: 4.382 ± 0.032	1.192GlyCys: 1.192 ± 0.016	3.442GlyAsp: 3.442 ± 0.029	3.199GlyGlu: 3.199 ± 0.029	2.815GlyPhe: 2.815 ± 0.025	6.488GlyGly: 6.488 ± 0.102	1.567GlyHis: 1.567 ± 0.017	3.201GlyIle: 3.201 ± 0.025	3.453GlyLys: 3.453 ± 0.032	5.452GlyLeu: 5.452 ± 0.038	1.633GlyMet: 1.633 ± 0.018	2.873GlyAsn: 2.873 ± 0.025	3.05GlyPro: 3.05 ± 0.035	2.59GlyGln: 2.59 ± 0.033	3.853GlyArg: 3.853 ± 0.027	5.847GlySer: 5.847 ± 0.047	3.859GlyThr: 3.859 ± 0.032	3.968GlyVal: 3.968 ± 0.029	0.828GlyTrp: 0.828 ± 0.013	2.02GlyTyr: 2.02 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	1.572HisAla: 1.572 ± 0.019	0.549HisCys: 0.549 ± 0.009	1.156HisAsp: 1.156 ± 0.015	1.154HisGlu: 1.154 ± 0.015	1.111HisPhe: 1.111 ± 0.013	1.655HisGly: 1.655 ± 0.017	1.207HisHis: 1.207 ± 0.026	1.091HisIle: 1.091 ± 0.012	0.904HisLys: 0.904 ± 0.013	2.306HisLeu: 2.306 ± 0.02	0.483HisMet: 0.483 ± 0.008	0.944HisAsn: 0.944 ± 0.011	1.563HisPro: 1.563 ± 0.019	1.096HisGln: 1.096 ± 0.016	1.625HisArg: 1.625 ± 0.019	1.912HisSer: 1.912 ± 0.02	1.154HisThr: 1.154 ± 0.016	1.503HisVal: 1.503 ± 0.015	0.313HisTrp: 0.313 ± 0.009	0.751HisTyr: 0.751 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	3.753IleAla: 3.753 ± 0.03	1.092IleCys: 1.092 ± 0.014	2.399IleAsp: 2.399 ± 0.021	2.324IleGlu: 2.324 ± 0.021	2.337IlePhe: 2.337 ± 0.026	2.986IleGly: 2.986 ± 0.024	1.201IleHis: 1.201 ± 0.013	2.646IleIle: 2.646 ± 0.042	2.176IleLys: 2.176 ± 0.021	4.806IleLeu: 4.806 ± 0.035	1.033IleMet: 1.033 ± 0.014	1.96IleAsn: 1.96 ± 0.02	2.97IlePro: 2.97 ± 0.022	1.93IleGln: 1.93 ± 0.02	3.011IleArg: 3.011 ± 0.022	3.83IleSer: 3.83 ± 0.025	2.911IleThr: 2.911 ± 0.023	3.435IleVal: 3.435 ± 0.038	0.636IleTrp: 0.636 ± 0.011	1.408IleTyr: 1.408 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.67LysAla: 3.67 ± 0.034	0.858LysCys: 0.858 ± 0.016	2.734LysAsp: 2.734 ± 0.026	3.215LysGlu: 3.215 ± 0.03	1.85LysPhe: 1.85 ± 0.018	2.498LysGly: 2.498 ± 0.03	1.064LysHis: 1.064 ± 0.015	2.519LysIle: 2.519 ± 0.024	3.611LysLys: 3.611 ± 0.039	4.404LysLeu: 4.404 ± 0.029	1.247LysMet: 1.247 ± 0.012	2.081LysAsn: 2.081 ± 0.017	2.631LysPro: 2.631 ± 0.033	1.929LysGln: 1.929 ± 0.017	3.321LysArg: 3.321 ± 0.031	3.7LysSer: 3.7 ± 0.032	3.062LysThr: 3.062 ± 0.025	3.002LysVal: 3.002 ± 0.025	0.623LysTrp: 0.623 ± 0.01	1.364LysTyr: 1.364 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	7.034LeuAla: 7.034 ± 0.036	1.611LeuCys: 1.611 ± 0.02	4.832LeuAsp: 4.832 ± 0.029	5.207LeuGlu: 5.207 ± 0.04	3.771LeuPhe: 3.771 ± 0.032	4.956LeuGly: 4.956 ± 0.031	2.335LeuHis: 2.335 ± 0.025	4.53LeuIle: 4.53 ± 0.032	4.655LeuLys: 4.655 ± 0.033	9.201LeuLeu: 9.201 ± 0.062	2.099LeuMet: 2.099 ± 0.021	3.666LeuAsn: 3.666 ± 0.024	5.402LeuPro: 5.402 ± 0.036	4.129LeuGln: 4.129 ± 0.032	5.765LeuArg: 5.765 ± 0.037	6.923LeuSer: 6.923 ± 0.036	5.593LeuThr: 5.593 ± 0.038	5.595LeuVal: 5.595 ± 0.033	1.021LeuTrp: 1.021 ± 0.013	2.378LeuTyr: 2.378 ± 0.021	0.0LeuXaa: 0.0 ± 0.0
Met	2.05MetAla: 2.05 ± 0.019	0.344MetCys: 0.344 ± 0.007	1.435MetAsp: 1.435 ± 0.014	1.625MetGlu: 1.625 ± 0.019	0.84MetPhe: 0.84 ± 0.012	1.344MetGly: 1.344 ± 0.017	0.47MetHis: 0.47 ± 0.009	1.032MetIle: 1.032 ± 0.014	1.233MetLys: 1.233 ± 0.015	1.928MetLeu: 1.928 ± 0.017	0.673MetMet: 0.673 ± 0.013	0.954MetAsn: 0.954 ± 0.013	1.189MetPro: 1.189 ± 0.014	0.936MetGln: 0.936 ± 0.015	1.25MetArg: 1.25 ± 0.014	1.746MetSer: 1.746 ± 0.015	1.457MetThr: 1.457 ± 0.014	1.411MetVal: 1.411 ± 0.016	0.263MetTrp: 0.263 ± 0.006	0.552MetTyr: 0.552 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.995AsnAla: 2.995 ± 0.024	0.823AsnCys: 0.823 ± 0.014	2.008AsnAsp: 2.008 ± 0.019	2.034AsnGlu: 2.034 ± 0.02	1.792AsnPhe: 1.792 ± 0.018	3.645AsnGly: 3.645 ± 0.035	0.971AsnHis: 0.971 ± 0.014	2.086AsnIle: 2.086 ± 0.019	1.614AsnLys: 1.614 ± 0.016	3.647AsnLeu: 3.647 ± 0.025	0.877AsnMet: 0.877 ± 0.015	1.988AsnAsn: 1.988 ± 0.026	2.351AsnPro: 2.351 ± 0.02	1.629AsnGln: 1.629 ± 0.021	2.407AsnArg: 2.407 ± 0.02	3.323AsnSer: 3.323 ± 0.028	1.982AsnThr: 1.982 ± 0.019	2.721AsnVal: 2.721 ± 0.022	0.566AsnTrp: 0.566 ± 0.011	1.232AsnTyr: 1.232 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	4.932ProAla: 4.932 ± 0.045	0.774ProCys: 0.774 ± 0.015	2.998ProAsp: 2.998 ± 0.02	3.352ProGlu: 3.352 ± 0.029	2.202ProPhe: 2.202 ± 0.022	3.504ProGly: 3.504 ± 0.041	1.226ProHis: 1.226 ± 0.016	2.413ProIle: 2.413 ± 0.021	2.588ProLys: 2.588 ± 0.026	4.535ProLeu: 4.535 ± 0.029	1.083ProMet: 1.083 ± 0.015	2.234ProAsn: 2.234 ± 0.019	4.616ProPro: 4.616 ± 0.05	2.204ProGln: 2.204 ± 0.029	2.916ProArg: 2.916 ± 0.025	5.329ProSer: 5.329 ± 0.045	3.731ProThr: 3.731 ± 0.035	4.151ProVal: 4.151 ± 0.034	0.586ProTrp: 0.586 ± 0.011	1.455ProTyr: 1.455 ± 0.017	0.001ProXaa: 0.001 ± 0.0
Gln	2.934GlnAla: 2.934 ± 0.025	0.743GlnCys: 0.743 ± 0.016	1.766GlnAsp: 1.766 ± 0.02	2.206GlnGlu: 2.206 ± 0.024	1.629GlnPhe: 1.629 ± 0.018	1.965GlnGly: 1.965 ± 0.025	1.175GlnHis: 1.175 ± 0.016	1.992GlnIle: 1.992 ± 0.019	2.038GlnLys: 2.038 ± 0.023	3.932GlnLeu: 3.932 ± 0.031	1.036GlnMet: 1.036 ± 0.013	1.713GlnAsn: 1.713 ± 0.019	2.501GlnPro: 2.501 ± 0.032	3.462GlnGln: 3.462 ± 0.072	2.712GlnArg: 2.712 ± 0.025	3.111GlnSer: 3.111 ± 0.026	2.492GlnThr: 2.492 ± 0.024	2.207GlnVal: 2.207 ± 0.019	0.493GlnTrp: 0.493 ± 0.009	1.162GlnTyr: 1.162 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	3.74ArgAla: 3.74 ± 0.025	1.132ArgCys: 1.132 ± 0.019	3.159ArgAsp: 3.159 ± 0.024	3.29ArgGlu: 3.29 ± 0.028	2.382ArgPhe: 2.382 ± 0.021	3.265ArgGly: 3.265 ± 0.027	1.592ArgHis: 1.592 ± 0.017	2.934ArgIle: 2.934 ± 0.019	3.617ArgLys: 3.617 ± 0.027	5.461ArgLeu: 5.461 ± 0.036	1.439ArgMet: 1.439 ± 0.014	2.762ArgAsn: 2.762 ± 0.021	3.389ArgPro: 3.389 ± 0.029	2.643ArgGln: 2.643 ± 0.028	4.743ArgArg: 4.743 ± 0.037	4.898ArgSer: 4.898 ± 0.038	3.548ArgThr: 3.548 ± 0.027	3.296ArgVal: 3.296 ± 0.024	0.741ArgTrp: 0.741 ± 0.011	1.608ArgTyr: 1.608 ± 0.017	0.0ArgXaa: 0.0 ± 0.0
Ser	6.626SerAla: 6.626 ± 0.041	1.442SerCys: 1.442 ± 0.019	4.338SerAsp: 4.338 ± 0.03	3.888SerGlu: 3.888 ± 0.03	3.233SerPhe: 3.233 ± 0.029	6.021SerGly: 6.021 ± 0.048	1.942SerHis: 1.942 ± 0.02	3.435SerIle: 3.435 ± 0.026	3.366SerLys: 3.366 ± 0.028	7.039SerLeu: 7.039 ± 0.037	1.661SerMet: 1.661 ± 0.018	3.093SerAsn: 3.093 ± 0.028	5.089SerPro: 5.089 ± 0.037	3.112SerGln: 3.112 ± 0.025	4.689SerArg: 4.689 ± 0.039	9.659SerSer: 9.659 ± 0.108	5.398SerThr: 5.398 ± 0.047	5.555SerVal: 5.555 ± 0.032	0.866SerTrp: 0.866 ± 0.012	2.091SerTyr: 2.091 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	5.515ThrAla: 5.515 ± 0.038	1.132ThrCys: 1.132 ± 0.018	3.265ThrAsp: 3.265 ± 0.026	3.207ThrGlu: 3.207 ± 0.031	2.562ThrPhe: 2.562 ± 0.024	4.084ThrGly: 4.084 ± 0.033	1.18ThrHis: 1.18 ± 0.014	2.972ThrIle: 2.972 ± 0.026	2.658ThrLys: 2.658 ± 0.024	5.159ThrLeu: 5.159 ± 0.034	1.216ThrMet: 1.216 ± 0.014	2.314ThrAsn: 2.314 ± 0.023	3.543ThrPro: 3.543 ± 0.026	1.941ThrGln: 1.941 ± 0.019	2.883ThrArg: 2.883 ± 0.021	5.183ThrSer: 5.183 ± 0.043	5.233ThrThr: 5.233 ± 0.088	4.853ThrVal: 4.853 ± 0.04	0.709ThrTrp: 0.709 ± 0.011	1.492ThrTyr: 1.492 ± 0.017	0.001ThrXaa: 0.001 ± 0.001
Val	5.568ValAla: 5.568 ± 0.033	1.406ValCys: 1.406 ± 0.02	3.59ValAsp: 3.59 ± 0.026	3.895ValGlu: 3.895 ± 0.029	2.868ValPhe: 2.868 ± 0.029	3.87ValGly: 3.87 ± 0.029	1.462ValHis: 1.462 ± 0.014	3.496ValIle: 3.496 ± 0.029	3.333ValLys: 3.333 ± 0.028	6.136ValLeu: 6.136 ± 0.04	1.57ValMet: 1.57 ± 0.015	2.574ValAsn: 2.574 ± 0.019	3.78ValPro: 3.78 ± 0.029	2.553ValGln: 2.553 ± 0.022	3.649ValArg: 3.649 ± 0.025	4.914ValSer: 4.914 ± 0.035	4.271ValThr: 4.271 ± 0.038	5.214ValVal: 5.214 ± 0.043	0.814ValTrp: 0.814 ± 0.012	1.895ValTyr: 1.895 ± 0.017	0.0ValXaa: 0.0 ± 0.0
Trp	0.707TrpAla: 0.707 ± 0.011	0.219TrpCys: 0.219 ± 0.006	0.649TrpAsp: 0.649 ± 0.011	0.614TrpGlu: 0.614 ± 0.01	0.495TrpPhe: 0.495 ± 0.01	0.561TrpGly: 0.561 ± 0.013	0.273TrpHis: 0.273 ± 0.007	0.749TrpIle: 0.749 ± 0.012	0.797TrpLys: 0.797 ± 0.012	1.173TrpLeu: 1.173 ± 0.016	0.375TrpMet: 0.375 ± 0.007	0.661TrpAsn: 0.661 ± 0.012	0.539TrpPro: 0.539 ± 0.01	0.48TrpGln: 0.48 ± 0.009	0.783TrpArg: 0.783 ± 0.012	0.965TrpSer: 0.965 ± 0.012	0.929TrpThr: 0.929 ± 0.014	0.607TrpVal: 0.607 ± 0.011	0.218TrpTrp: 0.218 ± 0.007	0.361TrpTyr: 0.361 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.892TyrAla: 1.892 ± 0.017	0.63TyrCys: 0.63 ± 0.01	1.521TyrAsp: 1.521 ± 0.016	1.448TyrGlu: 1.448 ± 0.018	1.355TyrPhe: 1.355 ± 0.014	2.154TyrGly: 2.154 ± 0.029	0.708TyrHis: 0.708 ± 0.011	1.305TyrIle: 1.305 ± 0.013	1.122TyrLys: 1.122 ± 0.015	2.581TyrLeu: 2.581 ± 0.022	0.578TyrMet: 0.578 ± 0.009	1.201TyrAsn: 1.201 ± 0.016	1.491TyrPro: 1.491 ± 0.02	1.168TyrGln: 1.168 ± 0.014	1.802TyrArg: 1.802 ± 0.02	2.087TyrSer: 2.087 ± 0.021	1.435TyrThr: 1.435 ± 0.019	1.752TyrVal: 1.752 ± 0.017	0.398TyrTrp: 0.398 ± 0.008	1.018TyrTyr: 1.018 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.024XaaXaa: 0.024 ± 0.004

Statistics based on 14867 proteins (6817457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---