Sclerotinia sclerotiorum fusarivirus 1
Average proteome isoelectric point is 8.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3BFZ2|A0A0G3BFZ2_9VIRU Uncharacterized protein OS=Sclerotinia sclerotiorum fusarivirus 1 OX=1661062 PE=4 SV=1
MM1 pKa = 8.07 DD2 pKa = 4.94 SFMQEE7 pKa = 2.78 VWRR10 pKa = 11.84 IFRR13 pKa = 11.84 SRR15 pKa = 11.84 SDD17 pKa = 3.08 VRR19 pKa = 11.84 LVKK22 pKa = 10.55 KK23 pKa = 10.31 ILSLGLIAPPPVGVTYY39 pKa = 10.29 YY40 pKa = 10.68 SNEE43 pKa = 3.45 GMLVGWSPKK52 pKa = 10.63 LEE54 pKa = 4.2 DD55 pKa = 3.35 EE56 pKa = 4.78 VYY58 pKa = 10.68 EE59 pKa = 3.94 KK60 pKa = 11.17 SKK62 pKa = 9.2 WLKK65 pKa = 9.41 RR66 pKa = 11.84 ALYY69 pKa = 8.32 TISYY73 pKa = 9.77 LFLFGMLLVLVLSSCLFSALFCGMIFGLFWCFLLHH108 pKa = 6.73 PFVCMGTLIALFFLVFVEE126 pKa = 4.37 MVSYY130 pKa = 11.05 SNVMHH135 pKa = 6.9 NIIMTTINTIRR146 pKa = 11.84 GKK148 pKa = 10.0 NFNVIGGSHH157 pKa = 5.9 NMISSIILNYY167 pKa = 9.72 FVSKK171 pKa = 10.39 RR172 pKa = 11.84 HH173 pKa = 5.18 VEE175 pKa = 3.67 ASFEE179 pKa = 3.96 YY180 pKa = 10.94 DD181 pKa = 3.18 EE182 pKa = 6.25 FFGTTNRR189 pKa = 11.84 VEE191 pKa = 4.71 LDD193 pKa = 3.44 TNANNYY199 pKa = 9.01 HH200 pKa = 6.51 WFDD203 pKa = 3.14 IPEE206 pKa = 4.09 YY207 pKa = 10.66 QEE209 pKa = 3.7 FDD211 pKa = 3.32 SRR213 pKa = 11.84 LVKK216 pKa = 10.38 IQMKK220 pKa = 10.11 YY221 pKa = 10.28 LDD223 pKa = 4.07 WYY225 pKa = 10.6 EE226 pKa = 3.96 KK227 pKa = 11.09 GLWQKK232 pKa = 9.07 LTALVIALVSLKK244 pKa = 10.43 FCSFLFKK251 pKa = 9.13 TTTKK255 pKa = 10.21 VAQKK259 pKa = 10.85 VITCLMLLAVVIGTGMLTPFTIFAMIDD286 pKa = 3.32 WIHH289 pKa = 6.45 YY290 pKa = 7.7 GIQTFFYY297 pKa = 10.77 NCVKK301 pKa = 10.39 LYY303 pKa = 10.71 NYY305 pKa = 9.41 VRR307 pKa = 11.84 SIIKK311 pKa = 10.05 NSLIIFASGLPEE323 pKa = 3.97 INEE326 pKa = 4.35 DD327 pKa = 3.2 GKK329 pKa = 9.52 TIIFKK334 pKa = 10.81 LDD336 pKa = 3.29 GTIKK340 pKa = 10.51 LSSFVHH346 pKa = 6.53 LKK348 pKa = 10.63 FKK350 pKa = 10.83 LLRR353 pKa = 11.84 LRR355 pKa = 11.84 WAIAVLRR362 pKa = 11.84 FLLRR366 pKa = 11.84 SQFIVDD372 pKa = 3.64 RR373 pKa = 11.84 KK374 pKa = 7.5 TANEE378 pKa = 3.94 KK379 pKa = 10.13 KK380 pKa = 10.26 FKK382 pKa = 9.95 FRR384 pKa = 11.84 SVFTSFSLEE393 pKa = 3.7 VTRR396 pKa = 11.84 KK397 pKa = 9.11 INEE400 pKa = 3.86 FNVPGFVRR408 pKa = 11.84 KK409 pKa = 9.35 FPSHH413 pKa = 7.05 LNRR416 pKa = 11.84 LDD418 pKa = 3.46 VQSTIDD424 pKa = 3.27 ILKK427 pKa = 10.64 DD428 pKa = 2.73 IGYY431 pKa = 9.12 PIGDD435 pKa = 3.49 VKK437 pKa = 10.87 AQRR440 pKa = 11.84 PNEE443 pKa = 4.21 TILGGRR449 pKa = 11.84 HH450 pKa = 5.7 NSWLQDD456 pKa = 3.07 GTNFITGLRR465 pKa = 11.84 ALKK468 pKa = 9.83 TYY470 pKa = 11.15 DD471 pKa = 3.3 FVEE474 pKa = 5.23 FNAFKK479 pKa = 10.89 LDD481 pKa = 3.44 VQEE484 pKa = 4.16 YY485 pKa = 9.2 RR486 pKa = 11.84 RR487 pKa = 11.84 SDD489 pKa = 3.41 SYY491 pKa = 10.75 VTIEE495 pKa = 4.31 NEE497 pKa = 3.94 LEE499 pKa = 4.16 SVSRR503 pKa = 11.84 YY504 pKa = 9.28 FNNKK508 pKa = 8.56 SVSIPKK514 pKa = 8.93 EE515 pKa = 3.81 QQVVDD520 pKa = 4.21 AVWEE524 pKa = 4.29 VVKK527 pKa = 10.77 EE528 pKa = 3.9 IFSDD532 pKa = 4.02 SKK534 pKa = 9.42 ITPIRR539 pKa = 11.84 SIYY542 pKa = 10.13 KK543 pKa = 9.47 KK544 pKa = 9.04 WKK546 pKa = 8.6 KK547 pKa = 10.25 NYY549 pKa = 9.79 NVGPLAPSSKK559 pKa = 9.89 LRR561 pKa = 11.84 KK562 pKa = 9.48 DD563 pKa = 3.29 GSMKK567 pKa = 10.48 KK568 pKa = 8.24 MRR570 pKa = 11.84 RR571 pKa = 11.84 TEE573 pKa = 4.53 DD574 pKa = 2.69 ISRR577 pKa = 11.84 FPTLTSYY584 pKa = 11.47 LNYY587 pKa = 9.39 WNNLYY592 pKa = 10.62 RR593 pKa = 11.84 HH594 pKa = 6.35 FPAMAMISSAFYY606 pKa = 10.42 KK607 pKa = 10.52 SEE609 pKa = 3.97 ALPEE613 pKa = 4.25 KK614 pKa = 9.65 KK615 pKa = 8.96 WRR617 pKa = 11.84 RR618 pKa = 11.84 NKK620 pKa = 9.47 VRR622 pKa = 11.84 TPIGSMLPQYY632 pKa = 9.51 LWQMVWSYY640 pKa = 11.23 EE641 pKa = 3.87 PNHH644 pKa = 6.07 RR645 pKa = 11.84 FKK647 pKa = 11.14 PNEE650 pKa = 3.82 TPIQIGLPLTGFHH663 pKa = 6.86 LSKK666 pKa = 10.71 LFEE669 pKa = 4.09 RR670 pKa = 11.84 HH671 pKa = 5.59 SKK673 pKa = 8.81 MKK675 pKa = 9.84 YY676 pKa = 9.82 HH677 pKa = 6.37 YY678 pKa = 10.95 AGDD681 pKa = 3.84 CTEE684 pKa = 4.45 FDD686 pKa = 3.46 STITKK691 pKa = 9.74 NVQRR695 pKa = 11.84 IIKK698 pKa = 8.88 SVRR701 pKa = 11.84 KK702 pKa = 9.85 KK703 pKa = 10.06 GFSRR707 pKa = 11.84 HH708 pKa = 5.25 RR709 pKa = 11.84 QFDD712 pKa = 4.22 MISEE716 pKa = 4.47 MIDD719 pKa = 2.95 INYY722 pKa = 9.82 DD723 pKa = 3.44 RR724 pKa = 11.84 LNEE727 pKa = 4.08 SLIMTPSTGNVYY739 pKa = 10.63 KK740 pKa = 10.7 KK741 pKa = 10.34 GTGLTTGHH749 pKa = 7.26 ASTSSDD755 pKa = 2.86 NSLALVSLYY764 pKa = 9.37 MAAWVNLTGLSAHH777 pKa = 6.13 EE778 pKa = 3.86 FKK780 pKa = 11.18 NFNEE784 pKa = 4.16 LSCYY788 pKa = 10.65 GDD790 pKa = 4.57 DD791 pKa = 4.34 NIFSIAEE798 pKa = 4.02 GAPKK802 pKa = 10.25 VWTFEE807 pKa = 4.32 NICNVLATWGVTMNNEE823 pKa = 4.11 VPDD826 pKa = 3.97 VDD828 pKa = 3.91 PTDD831 pKa = 3.74 LTKK834 pKa = 11.02 LPFLSKK840 pKa = 9.47 FCKK843 pKa = 10.01 RR844 pKa = 11.84 STVADD849 pKa = 3.21 QAYY852 pKa = 9.24 FRR854 pKa = 11.84 EE855 pKa = 4.3 KK856 pKa = 10.1 MGMNAPQFIVYY867 pKa = 9.67 HH868 pKa = 6.22 NPDD871 pKa = 3.4 ALLGKK876 pKa = 9.62 MKK878 pKa = 10.77 APVLNRR884 pKa = 11.84 DD885 pKa = 2.94 PRR887 pKa = 11.84 YY888 pKa = 9.9 KK889 pKa = 8.88 VTRR892 pKa = 11.84 VLSYY896 pKa = 10.62 MDD898 pKa = 4.28 LCAHH902 pKa = 6.0 NEE904 pKa = 3.32 KK905 pKa = 10.42 AYY907 pKa = 9.82 YY908 pKa = 9.31 VGRR911 pKa = 11.84 EE912 pKa = 4.07 VIEE915 pKa = 4.16 TLLFKK920 pKa = 11.25 YY921 pKa = 10.11 PDD923 pKa = 3.71 LAFMRR928 pKa = 11.84 ARR930 pKa = 11.84 IPSYY934 pKa = 10.3 DD935 pKa = 3.2 QVMEE939 pKa = 4.41 KK940 pKa = 10.24 FYY942 pKa = 11.34 SSNTSVRR949 pKa = 11.84 DD950 pKa = 3.34 PEE952 pKa = 4.04 EE953 pKa = 4.48 RR954 pKa = 11.84 DD955 pKa = 3.65 FEE957 pKa = 4.91 NFDD960 pKa = 3.73 PDD962 pKa = 5.32 DD963 pKa = 3.68 IVEE966 pKa = 4.18 YY967 pKa = 11.24 GSMTFMDD974 pKa = 4.58 YY975 pKa = 10.68 IFNYY979 pKa = 8.91 MSVVPDD985 pKa = 3.43 VLNPSIKK992 pKa = 10.24 NVGFGRR998 pKa = 11.84 TAQKK1002 pKa = 11.27 ALGFLTTWPRR1012 pKa = 11.84 QLLSKK1017 pKa = 10.88 SNGVFSEE1024 pKa = 4.25 GHH1026 pKa = 6.4 LSMLLQSSCYY1036 pKa = 10.67 DD1037 pKa = 4.04 FIEE1040 pKa = 4.73 DD1041 pKa = 4.52 RR1042 pKa = 11.84 SLTIQDD1048 pKa = 3.72 EE1049 pKa = 4.57 TDD1051 pKa = 2.91 LTLLVRR1057 pKa = 11.84 HH1058 pKa = 5.86 WLFLYY1063 pKa = 10.3 FRR1065 pKa = 11.84 LDD1067 pKa = 3.43 SEE1069 pKa = 4.87 KK1070 pKa = 10.67 YY1071 pKa = 10.75 NPIAWIDD1078 pKa = 3.36 WLLMKK1083 pKa = 10.04 FANVQFILNGKK1094 pKa = 8.32 IQTKK1098 pKa = 8.61 YY1099 pKa = 10.47 KK1100 pKa = 10.54 NYY1102 pKa = 10.5 SFPVWNLLLLGILNIVSIPDD1122 pKa = 3.22 IEE1124 pKa = 4.81 FIEE1127 pKa = 4.88 HH1128 pKa = 5.42 GTDD1131 pKa = 3.02 LKK1133 pKa = 11.2 FSVGEE1138 pKa = 4.11 SIMNFTLIDD1147 pKa = 3.34 PTYY1150 pKa = 9.77 WFGKK1154 pKa = 9.64 IYY1156 pKa = 10.89 NFVLNLIWDD1165 pKa = 3.77 QVPPNFKK1172 pKa = 10.31 NLQYY1176 pKa = 10.99 LEE1178 pKa = 4.72 NIEE1181 pKa = 4.42 HH1182 pKa = 6.74 HH1183 pKa = 6.77 GKK1185 pKa = 7.27 THH1187 pKa = 6.63 LVVAGTGTGKK1197 pKa = 8.34 STTMILYY1204 pKa = 8.53 LQSALGHH1211 pKa = 5.29 MFKK1214 pKa = 10.51 KK1215 pKa = 10.37 IIVIEE1220 pKa = 4.04 PRR1222 pKa = 11.84 SKK1224 pKa = 10.15 VVKK1227 pKa = 10.54 GLVSYY1232 pKa = 8.81 TKK1234 pKa = 10.16 TIGVQSSGLTSGLKK1248 pKa = 9.76 LDD1250 pKa = 3.66 QRR1252 pKa = 11.84 EE1253 pKa = 4.36 KK1254 pKa = 9.19 VWYY1257 pKa = 7.16 MTAQEE1262 pKa = 4.76 LLLNPQWVSKK1272 pKa = 10.94 EE1273 pKa = 3.8 NLFVLDD1279 pKa = 4.4 EE1280 pKa = 4.21 SHH1282 pKa = 7.63 IDD1284 pKa = 3.47 EE1285 pKa = 5.67 LPYY1288 pKa = 10.63 QVVKK1292 pKa = 10.84 QMIGAGSGLTTIMTTATPAQKK1313 pKa = 10.29 EE1314 pKa = 3.71 KK1315 pKa = 11.47 DD1316 pKa = 3.41 MAFTTTEE1323 pKa = 3.49 IEE1325 pKa = 4.01 LPKK1328 pKa = 10.16 IYY1330 pKa = 9.26 STKK1333 pKa = 10.27 LKK1335 pKa = 10.54 SYY1337 pKa = 10.01 NKK1339 pKa = 9.87 DD1340 pKa = 2.9 VEE1342 pKa = 4.55 VQVDD1346 pKa = 3.68 RR1347 pKa = 11.84 NNWCSRR1353 pKa = 11.84 YY1354 pKa = 10.14 LGIVTEE1360 pKa = 4.38 IMSSYY1365 pKa = 8.71 RR1366 pKa = 11.84 TPEE1369 pKa = 3.54 KK1370 pKa = 10.54 FLIFINDD1377 pKa = 3.54 KK1378 pKa = 11.09 ADD1380 pKa = 3.66 LEE1382 pKa = 4.59 VFSKK1386 pKa = 10.59 NVKK1389 pKa = 10.22 GKK1391 pKa = 10.54 GLFLSSEE1398 pKa = 4.28 TEE1400 pKa = 4.04 EE1401 pKa = 4.12 VDD1403 pKa = 5.0 LSEE1406 pKa = 5.57 DD1407 pKa = 3.38 CDD1409 pKa = 4.12 FVVTTAVCDD1418 pKa = 3.69 VAITIPGVTVVITPNFTRR1436 pKa = 11.84 NVQHH1440 pKa = 6.79 KK1441 pKa = 9.68 PDD1443 pKa = 3.8 GTTEE1447 pKa = 4.01 PVFVQIDD1454 pKa = 4.0 PSTLRR1459 pKa = 11.84 QRR1461 pKa = 11.84 MGRR1464 pKa = 11.84 TGRR1467 pKa = 11.84 TNNGDD1472 pKa = 3.37 AYY1474 pKa = 11.06 VINFKK1479 pKa = 11.0 GSFISQSLTINNEE1492 pKa = 3.72 GLLTEE1497 pKa = 4.66 WLAGGLDD1504 pKa = 3.56 INILGKK1510 pKa = 10.48 YY1511 pKa = 7.12 MPKK1514 pKa = 10.55 LFDD1517 pKa = 3.99 CFEE1520 pKa = 4.8 KK1521 pKa = 11.12 NLTTEE1526 pKa = 3.99 QLLTLTKK1533 pKa = 10.33 FIEE1536 pKa = 4.31 TNRR1539 pKa = 11.84 VDD1541 pKa = 3.95 KK1542 pKa = 11.13 FKK1544 pKa = 10.79 TKK1546 pKa = 9.94 QRR1548 pKa = 11.84 LKK1550 pKa = 10.82 NPDD1553 pKa = 4.05 EE1554 pKa = 4.34 IPPRR1558 pKa = 11.84 EE1559 pKa = 3.86 MNLFGIHH1566 pKa = 5.79 MEE1568 pKa = 4.98 GISGTSSGFSFIYY1581 pKa = 10.39 SDD1583 pKa = 4.38 LLNDD1587 pKa = 3.91 YY1588 pKa = 8.37 VTLLKK1593 pKa = 10.39 NAKK1596 pKa = 9.35 EE1597 pKa = 4.36 FVSKK1601 pKa = 9.95 TDD1603 pKa = 2.99 WSKK1606 pKa = 11.84 EE1607 pKa = 3.72 NLSLSKK1613 pKa = 10.94 ARR1615 pKa = 11.84 FARR1618 pKa = 11.84 DD1619 pKa = 3.19 NFLSSIGSRR1628 pKa = 11.84 GAEE1631 pKa = 3.84 GSAKK1635 pKa = 9.88 PVRR1638 pKa = 11.84 EE1639 pKa = 4.12 EE1640 pKa = 3.56 IYY1642 pKa = 8.41 GTKK1645 pKa = 10.01 RR1646 pKa = 11.84 QFHH1649 pKa = 6.25 LPEE1652 pKa = 4.34 TKK1654 pKa = 8.85 PTGRR1658 pKa = 11.84 PVRR1661 pKa = 11.84 KK1662 pKa = 10.02 GSFF1665 pKa = 3.03
Molecular weight: 191.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.287
IPC2_protein 8.317
IPC_protein 8.141
Toseland 8.712
ProMoST 8.697
Dawson 9.121
Bjellqvist 9.004
Wikipedia 9.268
Rodwell 9.282
Grimsley 9.121
Solomon 9.136
Lehninger 9.092
Nozaki 9.077
DTASelect 8.902
Thurlkill 9.004
EMBOSS 9.253
Sillero 9.18
Patrickios 4.495
IPC_peptide 9.136
IPC2_peptide 7.6
IPC2.peptide.svr19 7.631
Protein with the highest isoelectric point:
>tr|A0A0G3BAH4|A0A0G3BAH4_9VIRU RNA-directed RNA polymerase OS=Sclerotinia sclerotiorum fusarivirus 1 OX=1661062 PE=4 SV=1
MM1 pKa = 7.39 CGKK4 pKa = 10.11 RR5 pKa = 11.84 LRR7 pKa = 11.84 SQLSVGVSASHH18 pKa = 7.18 PKK20 pKa = 9.83 TGFTSITIAMEE31 pKa = 3.83 MRR33 pKa = 11.84 PSNVNVNSVGQSRR46 pKa = 11.84 QLNPSPLVGSPFDD59 pKa = 3.63 NANQKK64 pKa = 10.27 VGIPLVKK71 pKa = 9.52 TRR73 pKa = 11.84 HH74 pKa = 4.91
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 9.926
IPC_protein 11.257
Toseland 11.55
ProMoST 11.915
Dawson 11.55
Bjellqvist 11.462
Wikipedia 11.959
Rodwell 11.594
Grimsley 11.594
Solomon 11.974
Lehninger 11.886
Nozaki 11.535
DTASelect 11.462
Thurlkill 11.535
EMBOSS 12.018
Sillero 11.535
Patrickios 11.374
IPC_peptide 11.974
IPC2_peptide 10.921
IPC2.peptide.svr19 9.308
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2370
28
1665
592.5
67.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.966 ± 0.405
1.055 ± 0.255
4.895 ± 1.076
5.148 ± 1.157
5.907 ± 1.039
5.612 ± 0.637
1.646 ± 0.262
6.456 ± 0.575
8.059 ± 0.998
9.578 ± 0.786
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.616 ± 0.587
5.063 ± 0.827
4.726 ± 1.078
2.658 ± 0.639
4.937 ± 0.534
8.017 ± 0.964
6.582 ± 0.26
7.131 ± 0.526
2.068 ± 0.656
3.882 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here