Apis mellifera associated microvirus 58

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTT7|A0A3S8UTT7_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 58 OX=2494789 PE=4 SV=1
MM1 pKa = 7.38NLFNSVKK8 pKa = 7.63MTRR11 pKa = 11.84PKK13 pKa = 11.2GNTFDD18 pKa = 4.97LSHH21 pKa = 7.21DD22 pKa = 4.07LKK24 pKa = 11.23FSLDD28 pKa = 3.54AGKK31 pKa = 10.39LIPIINQAILPGDD44 pKa = 3.62SWNLSAAQMIRR55 pKa = 11.84VAPMIAPIMHH65 pKa = 6.46RR66 pKa = 11.84VDD68 pKa = 3.38VYY70 pKa = 8.41THH72 pKa = 6.56FFFVPNRR79 pKa = 11.84LLWKK83 pKa = 9.66GWQEE87 pKa = 4.59FITGGNGNDD96 pKa = 3.95PAPVHH101 pKa = 6.56PYY103 pKa = 10.45VILNNKK109 pKa = 5.73QTTVGKK115 pKa = 8.8LTDD118 pKa = 3.62YY119 pKa = 11.19LGIPTVPPASIPVPNPNTKK138 pKa = 9.88VSALDD143 pKa = 3.32IAAVGLVRR151 pKa = 11.84NEE153 pKa = 4.08YY154 pKa = 11.47YY155 pKa = 9.79MDD157 pKa = 4.01QNLQTPWDD165 pKa = 3.81VEE167 pKa = 4.54LNDD170 pKa = 4.64GDD172 pKa = 4.11NTSLLVAAGLIADD185 pKa = 4.4GTTGVGGDD193 pKa = 3.37PFRR196 pKa = 11.84RR197 pKa = 11.84SWEE200 pKa = 3.76HH201 pKa = 7.53DD202 pKa = 3.77YY203 pKa = 9.5FTAALPEE210 pKa = 4.29AQKK213 pKa = 11.22GPAATLPLGSEE224 pKa = 4.01ADD226 pKa = 3.84IIFDD230 pKa = 4.55PNGDD234 pKa = 3.63PSKK237 pKa = 11.29VFDD240 pKa = 3.72VSGNPLSINGHH251 pKa = 5.53LGKK254 pKa = 10.5SGSNDD259 pKa = 4.41LITEE263 pKa = 4.36DD264 pKa = 4.19ALNPGTFVDD273 pKa = 3.99TTLDD277 pKa = 3.1NSAQLKK283 pKa = 10.47ADD285 pKa = 4.3LSTATAATINVVRR298 pKa = 11.84LAFQLQRR305 pKa = 11.84WLEE308 pKa = 4.06RR309 pKa = 11.84NNIAGSRR316 pKa = 11.84YY317 pKa = 9.17IEE319 pKa = 4.4TILAHH324 pKa = 6.14FGVRR328 pKa = 11.84SSDD331 pKa = 3.19KK332 pKa = 10.06TLQRR336 pKa = 11.84PQYY339 pKa = 9.98LGGGKK344 pKa = 9.49SPVMISEE351 pKa = 4.27VLQTSEE357 pKa = 5.34SVTTPQGNLAGHH369 pKa = 6.93GLNVGQSHH377 pKa = 6.67NFTTFFEE384 pKa = 4.29EE385 pKa = 4.31HH386 pKa = 6.86GILLGFISVMPKK398 pKa = 9.73PAYY401 pKa = 9.52QQGLPRR407 pKa = 11.84NMFKK411 pKa = 10.43FDD413 pKa = 4.04RR414 pKa = 11.84IEE416 pKa = 4.19YY417 pKa = 7.9YY418 pKa = 9.65WPSFDD423 pKa = 5.77HH424 pKa = 7.79IGEE427 pKa = 3.99QAIKK431 pKa = 10.38GRR433 pKa = 11.84EE434 pKa = 3.68IFLTGDD440 pKa = 2.95QTYY443 pKa = 11.17DD444 pKa = 3.56DD445 pKa = 5.22KK446 pKa = 11.79DD447 pKa = 2.76WGYY450 pKa = 9.93IPRR453 pKa = 11.84YY454 pKa = 9.12SEE456 pKa = 3.48YY457 pKa = 10.21RR458 pKa = 11.84YY459 pKa = 9.98IPSRR463 pKa = 11.84VAGDD467 pKa = 3.58YY468 pKa = 11.17KK469 pKa = 10.29EE470 pKa = 4.38TLSYY474 pKa = 8.58WHH476 pKa = 7.49WGRR479 pKa = 11.84IFSNEE484 pKa = 3.73PEE486 pKa = 4.22LNEE489 pKa = 4.49EE490 pKa = 5.64FILCDD495 pKa = 3.19PTKK498 pKa = 10.7RR499 pKa = 11.84PFAVQDD505 pKa = 4.43LPDD508 pKa = 4.75SDD510 pKa = 5.61DD511 pKa = 3.99PNTPVRR517 pKa = 11.84DD518 pKa = 3.61WNTMYY523 pKa = 10.68CHH525 pKa = 6.86VVFQIKK531 pKa = 8.57ATRR534 pKa = 11.84RR535 pKa = 11.84MSKK538 pKa = 10.01YY539 pKa = 8.51ATPII543 pKa = 3.38

Molecular weight: 60.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTY7|A0A3S8UTY7_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 58 OX=2494789 PE=4 SV=1
MM1 pKa = 7.49CFEE4 pKa = 5.61PYY6 pKa = 9.17PLKK9 pKa = 11.04YY10 pKa = 10.22KK11 pKa = 10.22GAKK14 pKa = 9.28LHH16 pKa = 5.76LQGMTIPVPCGKK28 pKa = 10.13CPKK31 pKa = 8.62CTKK34 pKa = 10.46ARR36 pKa = 11.84INSWLFRR43 pKa = 11.84LTIHH47 pKa = 6.2ARR49 pKa = 11.84KK50 pKa = 9.52YY51 pKa = 9.66PYY53 pKa = 10.07PLFITLTYY61 pKa = 10.4DD62 pKa = 3.67DD63 pKa = 4.06KK64 pKa = 11.37HH65 pKa = 7.96LPVHH69 pKa = 6.3SKK71 pKa = 10.04SGRR74 pKa = 11.84VSLDD78 pKa = 2.85KK79 pKa = 10.99SDD81 pKa = 3.45PQKK84 pKa = 9.95WFKK87 pKa = 10.71RR88 pKa = 11.84LRR90 pKa = 11.84KK91 pKa = 9.97SLDD94 pKa = 3.15GQMDD98 pKa = 4.25LSYY101 pKa = 10.91FLVGEE106 pKa = 4.29YY107 pKa = 8.44GTKK110 pKa = 9.94RR111 pKa = 11.84KK112 pKa = 9.7RR113 pKa = 11.84PHH115 pKa = 5.26YY116 pKa = 10.13HH117 pKa = 4.97VLLYY121 pKa = 10.4GLPHH125 pKa = 6.45NKK127 pKa = 9.68SEE129 pKa = 5.46LIYY132 pKa = 9.24KK133 pKa = 8.33TWGLGFTMTLPVLDD147 pKa = 3.58GGMRR151 pKa = 11.84YY152 pKa = 8.56VLKK155 pKa = 11.14YY156 pKa = 8.09MAKK159 pKa = 9.53PRR161 pKa = 11.84SNYY164 pKa = 7.83TSKK167 pKa = 11.02LPEE170 pKa = 4.0FSLMSKK176 pKa = 10.71NIGANYY182 pKa = 7.71LTPQMIRR189 pKa = 11.84YY190 pKa = 8.67HH191 pKa = 8.25LEE193 pKa = 4.26DD194 pKa = 4.27PAHH197 pKa = 6.06TFASLGNGIKK207 pKa = 10.43LALPKK212 pKa = 10.33YY213 pKa = 8.45YY214 pKa = 9.97KK215 pKa = 10.46EE216 pKa = 4.44KK217 pKa = 10.63IFGIDD222 pKa = 2.86TPEE225 pKa = 4.01RR226 pKa = 11.84KK227 pKa = 9.3RR228 pKa = 11.84VTDD231 pKa = 3.58YY232 pKa = 10.61LQKK235 pKa = 10.09RR236 pKa = 11.84AEE238 pKa = 4.15MAQNSYY244 pKa = 10.4LRR246 pKa = 11.84RR247 pKa = 11.84LSLNNRR253 pKa = 11.84CLSQEE258 pKa = 4.25EE259 pKa = 4.37IIKK262 pKa = 10.49LAFLRR267 pKa = 11.84QHH269 pKa = 5.98SLSYY273 pKa = 10.85DD274 pKa = 3.06KK275 pKa = 10.89RR276 pKa = 11.84LQEE279 pKa = 4.37SFF281 pKa = 3.6

Molecular weight: 32.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1252

125

543

313.0

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.629 ± 0.818

0.639 ± 0.365

6.31 ± 1.12

4.872 ± 0.663

4.073 ± 0.452

6.629 ± 1.016

2.556 ± 0.875

5.112 ± 0.607

6.47 ± 1.44

9.824 ± 1.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.955 ± 0.511

5.751 ± 1.221

5.99 ± 0.928

4.633 ± 0.871

5.511 ± 0.594

6.15 ± 0.399

5.831 ± 0.742

4.313 ± 0.835

1.358 ± 0.403

4.393 ± 0.735
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski