Amycolatopsis acidiphila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A557ZZ67|A0A557ZZ67_9PSEU Uncharacterized protein OS=Amycolatopsis acidiphila OX=715473 GN=FNH06_31905 PE=4 SV=1
MM1 pKa = 7.16SQPIQTLHH9 pKa = 7.11DD10 pKa = 3.84FVLNLITDD18 pKa = 3.95DD19 pKa = 3.97SFGTSFLSDD28 pKa = 3.47PAGVLSGAGLGDD40 pKa = 3.51VTGLDD45 pKa = 3.7VQEE48 pKa = 4.39VTSLVADD55 pKa = 4.3HH56 pKa = 6.89LPAPVADD63 pKa = 3.53AVEE66 pKa = 4.52SGFAALPADD75 pKa = 3.65VTGVNDD81 pKa = 3.57LHH83 pKa = 6.89CALAHH88 pKa = 6.47LEE90 pKa = 4.16TVAAVAQDD98 pKa = 4.01LPVSVAGLSPSTTFAGDD115 pKa = 3.05AGGFTGAAALTGDD128 pKa = 4.69LVQTSGSLAGSTGGLAGGALADD150 pKa = 4.2TPAGAVTGAVAAATDD165 pKa = 4.06SVAAGVQSPLGTYY178 pKa = 10.04GVATDD183 pKa = 4.43SLPLSVPSFGSVSDD197 pKa = 3.97LGGTLDD203 pKa = 5.07SDD205 pKa = 4.28ALTHH209 pKa = 5.77GTSATAVAGGYY220 pKa = 8.35VASGGEE226 pKa = 3.8FAAAGIADD234 pKa = 3.96NSATLAGHH242 pKa = 7.17LSSVGDD248 pKa = 3.6VAAQPVAAGGDD259 pKa = 3.59EE260 pKa = 4.43LATHH264 pKa = 6.37VASGADD270 pKa = 3.34TLGDD274 pKa = 3.72VVSHH278 pKa = 6.06VPSVAAPAQLPADD291 pKa = 4.15LPVHH295 pKa = 6.57AVADD299 pKa = 4.65LPQSLPTPDD308 pKa = 3.66SVLPQVTHH316 pKa = 5.28TVEE319 pKa = 4.39STVSHH324 pKa = 6.82APLTDD329 pKa = 3.78SVSHH333 pKa = 6.53SSLIDD338 pKa = 3.65SVHH341 pKa = 6.24SALPDD346 pKa = 3.42VGDD349 pKa = 3.54LHH351 pKa = 7.01TDD353 pKa = 4.03LFHH356 pKa = 7.7GDD358 pKa = 3.95LPLGHH363 pKa = 7.28

Molecular weight: 34.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A558AAR2|A0A558AAR2_9PSEU Alpha/beta hydrolase OS=Amycolatopsis acidiphila OX=715473 GN=FNH06_17405 PE=4 SV=1
MM1 pKa = 7.37GRR3 pKa = 11.84RR4 pKa = 11.84ARR6 pKa = 11.84AAAWRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84ARR22 pKa = 11.84PPRR25 pKa = 11.84PRR27 pKa = 11.84RR28 pKa = 11.84PTVSASPQGPPQPPRR43 pKa = 11.84SPRR46 pKa = 11.84SPQPPRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84DD55 pKa = 3.44PVTGRR60 pKa = 11.84PTMHH64 pKa = 7.18

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7527

0

7527

2361779

26

2563

313.8

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.076 ± 0.036

0.801 ± 0.009

5.815 ± 0.024

5.788 ± 0.025

2.969 ± 0.014

9.304 ± 0.024

2.231 ± 0.013

3.536 ± 0.017

2.126 ± 0.018

10.584 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.728 ± 0.01

1.928 ± 0.014

5.825 ± 0.02

2.948 ± 0.017

7.887 ± 0.032

5.125 ± 0.02

5.86 ± 0.023

8.93 ± 0.026

1.474 ± 0.011

2.065 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski