Subsaximicrobium wynnwilliamsii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Subsaximicrobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6ZEX4|A0A5C6ZEX4_9FLAO tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB OS=Subsaximicrobium wynnwilliamsii OX=291179 GN=tsaB PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.36HH3 pKa = 5.51LTTLKK8 pKa = 10.87LIGFLILLLITSPVIHH24 pKa = 6.42AHH26 pKa = 6.19SLLSDD31 pKa = 4.22DD32 pKa = 5.18PLLDD36 pKa = 3.68TKK38 pKa = 11.55DD39 pKa = 5.19DD40 pKa = 3.69ITSSIDD46 pKa = 3.07PSVNVFIDD54 pKa = 3.42KK55 pKa = 10.78DD56 pKa = 3.94VLQAEE61 pKa = 4.43ALLNTTLNHH70 pKa = 7.14DD71 pKa = 4.38DD72 pKa = 4.02GVFHH76 pKa = 7.3LFTHH80 pKa = 6.65GKK82 pKa = 8.99PGQLLIDD89 pKa = 4.2GQWLQKK95 pKa = 10.69EE96 pKa = 4.48EE97 pKa = 3.96IVSFINSKK105 pKa = 10.74FNFPLGEE112 pKa = 3.98NSEE115 pKa = 4.3GQRR118 pKa = 11.84GLNIYY123 pKa = 9.6GCNFAQGEE131 pKa = 4.31KK132 pKa = 9.65GQKK135 pKa = 9.2AVAYY139 pKa = 10.05LEE141 pKa = 4.24KK142 pKa = 10.73QLGITIAASTNVTGKK157 pKa = 10.5DD158 pKa = 3.31GDD160 pKa = 3.76WQLEE164 pKa = 4.51SGTPTQLKK172 pKa = 10.17ALQNYY177 pKa = 9.12AYY179 pKa = 9.74NLQISCPNGLDD190 pKa = 3.16VHH192 pKa = 6.82IANDD196 pKa = 3.32VSGSVNSTEE205 pKa = 3.93FSQAKK210 pKa = 10.28DD211 pKa = 4.53FIAQLGASFDD221 pKa = 3.93NDD223 pKa = 3.1FGTLNTQSRR232 pKa = 11.84ISISNWSAGSNRR244 pKa = 11.84FVEE247 pKa = 4.27YY248 pKa = 10.33DD249 pKa = 3.78FPSAGQNYY257 pKa = 6.28TTSIADD263 pKa = 3.32ILSYY267 pKa = 10.73SSSSRR272 pKa = 11.84PFGGQTDD279 pKa = 4.35VYY281 pKa = 10.11TALLRR286 pKa = 11.84ANEE289 pKa = 4.23WVRR292 pKa = 11.84QNPVSDD298 pKa = 3.36RR299 pKa = 11.84DD300 pKa = 3.76VPKK303 pKa = 10.91VIVLLTDD310 pKa = 4.06ASCSQVANNISSLATQIKK328 pKa = 9.05NQGIYY333 pKa = 10.13IVVLAIDD340 pKa = 4.52DD341 pKa = 4.49AASCTALQGINVASAGGYY359 pKa = 9.79FSANNYY365 pKa = 7.57NTLQQDD371 pKa = 4.72AITFIQNIATASCDD385 pKa = 3.33NGGPAFFDD393 pKa = 3.61LTVSLSDD400 pKa = 5.65FVITDD405 pKa = 3.78CNTTAVASANYY416 pKa = 7.95TVSNISSGDD425 pKa = 3.52DD426 pKa = 3.42FNANLQVSFYY436 pKa = 10.87NGDD439 pKa = 3.32PTQPEE444 pKa = 3.99TQYY447 pKa = 11.36IFTDD451 pKa = 3.34NAGTQNLANGMGTYY465 pKa = 10.5SSTVNSDD472 pKa = 3.14ALINTSTLYY481 pKa = 11.02AVVNFDD487 pKa = 4.45GSLPGNAVPLSLSDD501 pKa = 4.48LSNQINVANEE511 pKa = 2.96GRR513 pKa = 11.84TFNNISTPVTRR524 pKa = 11.84DD525 pKa = 3.33DD526 pKa = 4.69GAGCQPFSTIDD537 pKa = 3.52VQVSNSGVGCDD548 pKa = 4.51DD549 pKa = 3.41EE550 pKa = 5.9VIYY553 pKa = 9.62TLQICNTGTADD564 pKa = 4.16ALMSPSDD571 pKa = 4.27IIPYY575 pKa = 10.05APAGFSLSSSTLIGTDD591 pKa = 3.18PFDD594 pKa = 3.91NLTGITASQLGQDD607 pKa = 3.72IDD609 pKa = 4.41GEE611 pKa = 4.32ALGDD615 pKa = 3.64GSGASVSMSADD626 pKa = 3.04GTTVAIGASANDD638 pKa = 3.59VNGSNSGHH646 pKa = 4.65VRR648 pKa = 11.84AYY650 pKa = 10.1HH651 pKa = 6.54LSGGTWNQRR660 pKa = 11.84GQDD663 pKa = 3.4IDD665 pKa = 3.95GEE667 pKa = 4.43AAFDD671 pKa = 3.53SSGFSVSMSADD682 pKa = 3.21GNTVAIGAIGTDD694 pKa = 3.81GNGSNSGHH702 pKa = 5.34VRR704 pKa = 11.84LYY706 pKa = 10.69DD707 pKa = 3.31WSGTAWIQRR716 pKa = 11.84GLDD719 pKa = 3.22IDD721 pKa = 4.35GEE723 pKa = 4.41AAGDD727 pKa = 3.6SSGFSVSMTSDD738 pKa = 3.1GNTLAIGARR747 pKa = 11.84NNDD750 pKa = 3.52GNTGIDD756 pKa = 3.38TDD758 pKa = 3.6NRR760 pKa = 11.84GHH762 pKa = 5.6VRR764 pKa = 11.84IYY766 pKa = 11.02DD767 pKa = 3.51WAGGSWNQRR776 pKa = 11.84GLDD779 pKa = 3.47IDD781 pKa = 4.29GEE783 pKa = 4.4AAGDD787 pKa = 3.58EE788 pKa = 4.74SGWSVSMSVDD798 pKa = 3.1GNTVAIGATDD808 pKa = 3.51NDD810 pKa = 4.66SNGSKK815 pKa = 9.95SGHH818 pKa = 4.23VRR820 pKa = 11.84IYY822 pKa = 10.7DD823 pKa = 3.4WSGTAWTQRR832 pKa = 11.84GLDD835 pKa = 3.25IDD837 pKa = 4.44GEE839 pKa = 4.27NSNDD843 pKa = 3.22EE844 pKa = 4.61SGWSVSISADD854 pKa = 3.25GNTVAIGAIDD864 pKa = 3.67NDD866 pKa = 4.19GGGGSSGHH874 pKa = 4.48VRR876 pKa = 11.84VYY878 pKa = 10.0EE879 pKa = 3.94WSGTAWTQRR888 pKa = 11.84GLDD891 pKa = 3.6IDD893 pKa = 5.48GEE895 pKa = 4.54DD896 pKa = 3.83NDD898 pKa = 5.34DD899 pKa = 3.52EE900 pKa = 5.01SGYY903 pKa = 9.83SVSMSADD910 pKa = 3.19GNTVATGAHH919 pKa = 6.2RR920 pKa = 11.84NDD922 pKa = 3.24GNAFRR927 pKa = 11.84AGHH930 pKa = 5.17VRR932 pKa = 11.84VYY934 pKa = 10.47DD935 pKa = 3.49WSGGSWTQRR944 pKa = 11.84GLDD947 pKa = 3.24IDD949 pKa = 4.4GEE951 pKa = 4.43AFGDD955 pKa = 3.75YY956 pKa = 10.26SGRR959 pKa = 11.84SVSMSADD966 pKa = 3.26GNTVAIGAPSNDD978 pKa = 3.25GNGTSSGHH986 pKa = 4.5VRR988 pKa = 11.84VYY990 pKa = 10.9SYY992 pKa = 11.86GSGAEE997 pKa = 3.95ILPASTCATYY1007 pKa = 10.34EE1008 pKa = 3.61YY1009 pKa = 9.46TYY1011 pKa = 10.8NITGVAATAGTPYY1024 pKa = 10.57DD1025 pKa = 3.53YY1026 pKa = 11.43SLAVNASAQSGNDD1039 pKa = 3.03PVTFTPNTNFSADD1052 pKa = 3.38GNTGLNGFDD1061 pKa = 3.22GAVNNTDD1068 pKa = 3.44DD1069 pKa = 3.77LTYY1072 pKa = 11.12DD1073 pKa = 3.94GVTTPCTAGDD1083 pKa = 3.79QIAVSVSMTGDD1094 pKa = 4.22GSCPGSFSTATVTVEE1109 pKa = 4.03NTSGTTISHH1118 pKa = 7.21ALLTLDD1124 pKa = 5.33LSGTDD1129 pKa = 2.82AFYY1132 pKa = 11.17NGEE1135 pKa = 4.47PYY1137 pKa = 11.01NFTNGMVLEE1146 pKa = 4.7EE1147 pKa = 4.72PNIFDD1152 pKa = 3.87PAYY1155 pKa = 9.08PAVPNALSNQTGSQQLAIFALPDD1178 pKa = 3.44GTSTFEE1184 pKa = 4.13LDD1186 pKa = 3.62VAVGTSVIDD1195 pKa = 3.96LSATIAQIPTNLNAGGSAIGSAGIAPAVEE1224 pKa = 4.44PSISGTPPGDD1234 pKa = 3.22VTTAATTIALSGYY1247 pKa = 9.75AATNATSVSWTSGSNGSFANASTASTTYY1275 pKa = 10.09TINDD1279 pKa = 3.05QDD1281 pKa = 3.26RR1282 pKa = 11.84AYY1284 pKa = 10.76GFVEE1288 pKa = 4.66LSLEE1292 pKa = 4.16ALSSGGCSTLTSFRR1306 pKa = 11.84VNITGGTYY1314 pKa = 10.1DD1315 pKa = 3.66YY1316 pKa = 11.67GDD1318 pKa = 3.94AQSSFDD1324 pKa = 3.71SGEE1327 pKa = 4.02TQLPVAGAALISNDD1341 pKa = 3.44VFLGTIPADD1350 pKa = 3.58AEE1352 pKa = 3.93AAAQPTADD1360 pKa = 3.76ATGDD1364 pKa = 3.72GADD1367 pKa = 3.75EE1368 pKa = 5.87DD1369 pKa = 4.59GLQTLTFGNFQPGNLYY1385 pKa = 9.04TLSVDD1390 pKa = 3.27ATNNSAALAYY1400 pKa = 9.81LHH1402 pKa = 7.27AYY1404 pKa = 8.96IDD1406 pKa = 3.81WNDD1409 pKa = 3.81DD1410 pKa = 2.94GDD1412 pKa = 4.43FIGDD1416 pKa = 3.63EE1417 pKa = 4.57GEE1419 pKa = 4.38KK1420 pKa = 10.57SLLVNVPAGSGAATYY1435 pKa = 8.78EE1436 pKa = 4.18VNFIIPSNGAAFTSSTIVRR1455 pKa = 11.84LRR1457 pKa = 11.84LSSDD1461 pKa = 2.83EE1462 pKa = 4.15FAAGRR1467 pKa = 11.84SYY1469 pKa = 11.01GAAANGEE1476 pKa = 4.2IEE1478 pKa = 5.46DD1479 pKa = 4.06FLIDD1483 pKa = 3.41EE1484 pKa = 4.53TLSVEE1489 pKa = 3.95NLEE1492 pKa = 4.45AKK1494 pKa = 10.37GISIYY1499 pKa = 10.32PNPASDD1505 pKa = 4.39FLIIDD1510 pKa = 4.21FGQNAGRR1517 pKa = 11.84FNTVEE1522 pKa = 4.48LYY1524 pKa = 10.64DD1525 pKa = 3.24ITGRR1529 pKa = 11.84LMSKK1533 pKa = 9.42TSLKK1537 pKa = 10.47EE1538 pKa = 3.77NPLDD1542 pKa = 3.69KK1543 pKa = 11.0VQVRR1547 pKa = 11.84IDD1549 pKa = 3.39QLNAGIYY1556 pKa = 10.18LLNLSSKK1563 pKa = 10.3EE1564 pKa = 3.93DD1565 pKa = 3.4QFSQRR1570 pKa = 11.84VIIRR1574 pKa = 3.56

Molecular weight: 163.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6ZE19|A0A5C6ZE19_9FLAO Xanthine dehydrogenase family protein molybdopterin-binding subunit OS=Subsaximicrobium wynnwilliamsii OX=291179 GN=ESY86_13270 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.05GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.41VSVSSEE48 pKa = 3.83VRR50 pKa = 11.84HH51 pKa = 6.04KK52 pKa = 10.77KK53 pKa = 10.01

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3955

0

3955

1316798

37

3305

332.9

37.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.762 ± 0.045

0.757 ± 0.014

5.814 ± 0.034

6.589 ± 0.041

5.293 ± 0.039

6.203 ± 0.045

1.803 ± 0.021

7.718 ± 0.038

7.463 ± 0.054

9.434 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.021

6.062 ± 0.041

3.299 ± 0.022

3.595 ± 0.023

3.467 ± 0.029

6.67 ± 0.031

5.784 ± 0.045

6.039 ± 0.031

1.011 ± 0.015

3.981 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski