Archangium gephyra
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10110 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G2ZY50|A0A0G2ZY50_9DELT 4-hydroxybutyrate coenzyme A transferase OS=Archangium gephyra OX=48 GN=AA314_06071 PE=3 SV=1
MM1 pKa = 7.8 ALGDD5 pKa = 3.71 FNGDD9 pKa = 2.42 HH10 pKa = 6.62 RR11 pKa = 11.84 AEE13 pKa = 4.08 VFRR16 pKa = 11.84 PDD18 pKa = 3.78 GANWWLASPNPGSWSYY34 pKa = 11.87 LNVSASTLANTRR46 pKa = 11.84 LADD49 pKa = 3.52 MNGDD53 pKa = 3.45 GKK55 pKa = 11.42 VDD57 pKa = 3.47 VFHH60 pKa = 7.66 ANGTGWWVSYY70 pKa = 10.99 GGTTGWNFLASSSLTVSQVALGDD93 pKa = 3.95 FNGDD97 pKa = 2.88 GQADD101 pKa = 3.65 VFYY104 pKa = 10.46 PDD106 pKa = 3.51 GANWWVFYY114 pKa = 10.7 SGSGVQTYY122 pKa = 10.81 LNVSASTLANTRR134 pKa = 11.84 LADD137 pKa = 3.52 MNGDD141 pKa = 3.45 GKK143 pKa = 11.42 VDD145 pKa = 3.47 VFHH148 pKa = 7.66 ANGTGWWVSYY158 pKa = 10.99 GGTTGWNFLASSSLTVSQVALGDD181 pKa = 4.05 LNGDD185 pKa = 3.44 GQADD189 pKa = 3.63 VFYY192 pKa = 10.46 PDD194 pKa = 3.78 GANWWLFPSGSGGPAFLHH212 pKa = 6.15 VSASTLANTEE222 pKa = 4.06 LADD225 pKa = 3.45 VDD227 pKa = 3.73 GDD229 pKa = 3.43 GRR231 pKa = 11.84 TDD233 pKa = 3.09 VFHH236 pKa = 7.73 ANGTGWWVSYY246 pKa = 11.11 GGVTDD251 pKa = 3.74 WNFLASSSYY260 pKa = 10.31 TFF262 pKa = 3.91
Molecular weight: 27.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.884
IPC_protein 3.91
Toseland 3.668
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.935
Rodwell 3.732
Grimsley 3.579
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.406
Thurlkill 3.745
EMBOSS 3.935
Sillero 4.05
Patrickios 1.1
IPC_peptide 3.923
IPC2_peptide 4.012
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|A0A0G2ZAC6|A0A0G2ZAC6_9DELT General secretion pathway protein G OS=Archangium gephyra OX=48 GN=AA314_00192 PE=4 SV=1
MM1 pKa = 7.38 VSPAGPFPRR10 pKa = 11.84 RR11 pKa = 11.84 VGLKK15 pKa = 9.89 LGAVVARR22 pKa = 11.84 VSLRR26 pKa = 11.84 SNGGLAGRR34 pKa = 11.84 SLRR37 pKa = 4.06
Molecular weight: 3.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.437
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.121
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10110
0
10110
3715422
37
12217
367.5
39.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.406 ± 0.034
0.942 ± 0.012
4.765 ± 0.018
6.803 ± 0.034
3.34 ± 0.014
8.834 ± 0.03
2.154 ± 0.013
2.862 ± 0.016
2.922 ± 0.023
11.343 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.937 ± 0.011
2.169 ± 0.015
6.164 ± 0.025
3.506 ± 0.017
8.025 ± 0.03
5.845 ± 0.026
5.459 ± 0.028
7.914 ± 0.023
1.441 ± 0.011
2.169 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here