Halomonas sp. es.049

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0GDB6|A0A2N0GDB6_9GAMM Fanconi-associated nuclease OS=Halomonas sp. es.049 OX=1761788 GN=ATG69_3493 PE=3 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84LTLIATATLAATTTLSTYY20 pKa = 10.75AIADD24 pKa = 3.4NHH26 pKa = 6.73GDD28 pKa = 3.51HH29 pKa = 7.28TDD31 pKa = 3.55TNDD34 pKa = 3.07QALMIVTSDD43 pKa = 3.83AIEE46 pKa = 4.27TQSMALILGNAMHH59 pKa = 6.16QQGTDD64 pKa = 3.38LHH66 pKa = 7.27VLLCDD71 pKa = 3.44AAGDD75 pKa = 4.06LAVDD79 pKa = 5.35GYY81 pKa = 11.78ASNAPINTPPGNPMEE96 pKa = 4.42KK97 pKa = 10.03LAPEE101 pKa = 4.81GLLKK105 pKa = 10.09MLMGEE110 pKa = 4.29GVSVDD115 pKa = 3.04VCAIYY120 pKa = 10.58LPNTDD125 pKa = 3.55YY126 pKa = 11.86AEE128 pKa = 5.24DD129 pKa = 4.29DD130 pKa = 3.87LLEE133 pKa = 4.56GVGVAAPGPMAEE145 pKa = 4.39MMRR148 pKa = 11.84DD149 pKa = 3.12PAIPVFSFF157 pKa = 3.42

Molecular weight: 16.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0G8D3|A0A2N0G8D3_9GAMM Transport permease protein OS=Halomonas sp. es.049 OX=1761788 GN=ATG69_1658 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.07NGRR28 pKa = 11.84AIITRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3282

0

3282

1130750

37

8810

344.5

37.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.736 ± 0.127

0.902 ± 0.017

6.028 ± 0.158

6.184 ± 0.056

3.499 ± 0.036

7.701 ± 0.077

2.423 ± 0.035

5.02 ± 0.039

3.098 ± 0.042

11.0 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.031

3.238 ± 0.067

4.666 ± 0.037

4.496 ± 0.038

6.164 ± 0.072

5.945 ± 0.042

5.395 ± 0.079

7.058 ± 0.042

1.445 ± 0.023

2.425 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski