Macrococcus bohemicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Macrococcus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328A501|A0A328A501_9STAP Phosphopentomutase OS=Macrococcus bohemicus OX=1903056 GN=deoB PE=3 SV=1
MM1 pKa = 7.14GHH3 pKa = 7.16IIFYY7 pKa = 7.18TQPNCSLCDD16 pKa = 3.73DD17 pKa = 4.72AKK19 pKa = 11.03LQISFAQEE27 pKa = 4.07DD28 pKa = 3.86INIKK32 pKa = 7.9VTEE35 pKa = 4.1VNIQQDD41 pKa = 3.55DD42 pKa = 4.16VLNEE46 pKa = 4.33LYY48 pKa = 10.49CLSVPVLIDD57 pKa = 3.29KK58 pKa = 8.26DD59 pKa = 3.6TGEE62 pKa = 4.74TIQEE66 pKa = 4.06GNIDD70 pKa = 4.23FVTIINFFEE79 pKa = 4.39SKK81 pKa = 10.95YY82 pKa = 10.08EE83 pKa = 3.84

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328A5J2|A0A328A5J2_9STAP Universal stress protein OS=Macrococcus bohemicus OX=1903056 GN=BHX94_04375 PE=3 SV=1
MM1 pKa = 7.3ARR3 pKa = 11.84IAGVDD8 pKa = 3.45VPRR11 pKa = 11.84EE12 pKa = 3.9KK13 pKa = 10.48RR14 pKa = 11.84VVIALTYY21 pKa = 10.35VYY23 pKa = 10.75GIGKK27 pKa = 6.57TTAQKK32 pKa = 10.44ILAAANVNEE41 pKa = 4.37STRR44 pKa = 11.84VRR46 pKa = 11.84DD47 pKa = 3.64LTEE50 pKa = 5.34DD51 pKa = 3.2EE52 pKa = 4.46LNRR55 pKa = 11.84IRR57 pKa = 11.84EE58 pKa = 3.99QVDD61 pKa = 4.13GYY63 pKa = 10.99KK64 pKa = 10.07VEE66 pKa = 4.32GDD68 pKa = 3.36LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.5INLNIKK78 pKa = 10.14RR79 pKa = 11.84LMEE82 pKa = 3.99IASYY86 pKa = 10.45RR87 pKa = 11.84GIRR90 pKa = 11.84HH91 pKa = 5.96RR92 pKa = 11.84RR93 pKa = 11.84GLPTRR98 pKa = 11.84GQNTKK103 pKa = 10.61NNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 10.16GPVKK114 pKa = 9.88TVANKK119 pKa = 10.03KK120 pKa = 9.77KK121 pKa = 10.67

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2430

0

2430

708472

22

3929

291.6

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.394 ± 0.049

0.653 ± 0.015

5.78 ± 0.047

7.103 ± 0.056

4.571 ± 0.045

6.149 ± 0.05

2.234 ± 0.026

8.811 ± 0.055

7.561 ± 0.055

9.253 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.951 ± 0.031

5.342 ± 0.04

3.188 ± 0.031

3.548 ± 0.031

3.544 ± 0.04

5.745 ± 0.063

5.735 ± 0.099

6.739 ± 0.044

0.724 ± 0.015

3.975 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski