Amino acid dipepetide frequency for Macrococcus bohemicus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.398AlaAla: 4.398 ± 0.113
0.56AlaCys: 0.56 ± 0.029
3.136AlaAsp: 3.136 ± 0.084
3.968AlaGlu: 3.968 ± 0.085
2.995AlaPhe: 2.995 ± 0.068
4.256AlaGly: 4.256 ± 0.097
1.428AlaHis: 1.428 ± 0.053
6.075AlaIle: 6.075 ± 0.12
4.552AlaLys: 4.552 ± 0.091
6.834AlaLeu: 6.834 ± 0.108
2.154AlaMet: 2.154 ± 0.054
2.644AlaAsn: 2.644 ± 0.06
1.865AlaPro: 1.865 ± 0.069
2.274AlaGln: 2.274 ± 0.064
2.366AlaArg: 2.366 ± 0.069
3.678AlaSer: 3.678 ± 0.133
3.52AlaThr: 3.52 ± 0.086
4.617AlaVal: 4.617 ± 0.092
0.401AlaTrp: 0.401 ± 0.025
2.215AlaTyr: 2.215 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.387CysAla: 0.387 ± 0.026
0.086CysCys: 0.086 ± 0.012
0.401CysAsp: 0.401 ± 0.028
0.39CysGlu: 0.39 ± 0.024
0.279CysPhe: 0.279 ± 0.021
0.61CysGly: 0.61 ± 0.032
0.193CysHis: 0.193 ± 0.017
0.579CysIle: 0.579 ± 0.03
0.342CysLys: 0.342 ± 0.018
0.594CysLeu: 0.594 ± 0.03
0.174CysMet: 0.174 ± 0.016
0.323CysAsn: 0.323 ± 0.018
0.294CysPro: 0.294 ± 0.024
0.174CysGln: 0.174 ± 0.017
0.199CysArg: 0.199 ± 0.017
0.39CysSer: 0.39 ± 0.024
0.402CysThr: 0.402 ± 0.025
0.416CysVal: 0.416 ± 0.026
0.044CysTrp: 0.044 ± 0.008
0.258CysTyr: 0.258 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
4.058AspAla: 4.058 ± 0.076
0.387AspCys: 0.387 ± 0.025
3.197AspAsp: 3.197 ± 0.077
4.983AspGlu: 4.983 ± 0.103
2.759AspPhe: 2.759 ± 0.062
3.338AspGly: 3.338 ± 0.087
1.157AspHis: 1.157 ± 0.041
5.261AspIle: 5.261 ± 0.089
4.728AspLys: 4.728 ± 0.113
5.045AspLeu: 5.045 ± 0.089
1.526AspMet: 1.526 ± 0.041
2.998AspAsn: 2.998 ± 0.067
1.519AspPro: 1.519 ± 0.061
1.667AspGln: 1.667 ± 0.05
1.941AspArg: 1.941 ± 0.054
2.824AspSer: 2.824 ± 0.141
3.108AspThr: 3.108 ± 0.069
4.088AspVal: 4.088 ± 0.082
0.449AspTrp: 0.449 ± 0.028
2.771AspTyr: 2.771 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
4.915GluAla: 4.915 ± 0.089
0.323GluCys: 0.323 ± 0.023
4.006GluAsp: 4.006 ± 0.076
5.532GluGlu: 5.532 ± 0.112
2.418GluPhe: 2.418 ± 0.063
3.599GluGly: 3.599 ± 0.086
1.667GluHis: 1.667 ± 0.054
6.036GluIle: 6.036 ± 0.103
6.146GluLys: 6.146 ± 0.119
6.189GluLeu: 6.189 ± 0.121
2.333GluMet: 2.333 ± 0.059
3.762GluAsn: 3.762 ± 0.074
1.66GluPro: 1.66 ± 0.055
3.108GluGln: 3.108 ± 0.07
3.4GluArg: 3.4 ± 0.081
3.75GluSer: 3.75 ± 0.076
4.095GluThr: 4.095 ± 0.074
4.994GluVal: 4.994 ± 0.091
0.553GluTrp: 0.553 ± 0.03
2.542GluTyr: 2.542 ± 0.067
0.0GluXaa: 0.0 ± 0.0
Phe
2.568PheAla: 2.568 ± 0.062
0.303PheCys: 0.303 ± 0.021
2.92PheAsp: 2.92 ± 0.062
3.256PheGlu: 3.256 ± 0.072
2.141PhePhe: 2.141 ± 0.068
3.162PheGly: 3.162 ± 0.075
0.917PheHis: 0.917 ± 0.034
4.609PheIle: 4.609 ± 0.107
3.368PheLys: 3.368 ± 0.07
3.901PheLeu: 3.901 ± 0.101
1.292PheMet: 1.292 ± 0.041
2.957PheAsn: 2.957 ± 0.068
1.306PhePro: 1.306 ± 0.043
1.231PheGln: 1.231 ± 0.045
1.307PheArg: 1.307 ± 0.043
2.8PheSer: 2.8 ± 0.067
2.422PheThr: 2.422 ± 0.06
3.119PheVal: 3.119 ± 0.067
0.336PheTrp: 0.336 ± 0.022
1.79PheTyr: 1.79 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.21GlyAla: 4.21 ± 0.098
0.486GlyCys: 0.486 ± 0.03
3.117GlyAsp: 3.117 ± 0.081
3.901GlyGlu: 3.901 ± 0.091
3.006GlyPhe: 3.006 ± 0.066
4.189GlyGly: 4.189 ± 0.092
1.365GlyHis: 1.365 ± 0.039
5.75GlyIle: 5.75 ± 0.106
4.695GlyLys: 4.695 ± 0.086
5.684GlyLeu: 5.684 ± 0.095
1.838GlyMet: 1.838 ± 0.055
2.699GlyAsn: 2.699 ± 0.067
1.483GlyPro: 1.483 ± 0.048
2.02GlyGln: 2.02 ± 0.052
2.239GlyArg: 2.239 ± 0.065
3.32GlySer: 3.32 ± 0.064
3.697GlyThr: 3.697 ± 0.126
4.477GlyVal: 4.477 ± 0.087
0.507GlyTrp: 0.507 ± 0.028
2.812GlyTyr: 2.812 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.338HisAla: 1.338 ± 0.042
0.137HisCys: 0.137 ± 0.016
1.265HisAsp: 1.265 ± 0.053
1.476HisGlu: 1.476 ± 0.042
1.16HisPhe: 1.16 ± 0.052
1.351HisGly: 1.351 ± 0.044
0.738HisHis: 0.738 ± 0.037
1.944HisIle: 1.944 ± 0.052
1.241HisLys: 1.241 ± 0.042
2.169HisLeu: 2.169 ± 0.057
0.584HisMet: 0.584 ± 0.027
1.088HisAsn: 1.088 ± 0.042
1.095HisPro: 1.095 ± 0.045
0.913HisGln: 0.913 ± 0.034
0.8HisArg: 0.8 ± 0.033
1.088HisSer: 1.088 ± 0.047
1.21HisThr: 1.21 ± 0.044
1.503HisVal: 1.503 ± 0.042
0.159HisTrp: 0.159 ± 0.015
1.083HisTyr: 1.083 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
6.202IleAla: 6.202 ± 0.102
0.69IleCys: 0.69 ± 0.032
5.794IleAsp: 5.794 ± 0.106
6.809IleGlu: 6.809 ± 0.11
3.795IlePhe: 3.795 ± 0.103
5.721IleGly: 5.721 ± 0.113
1.841IleHis: 1.841 ± 0.054
7.684IleIle: 7.684 ± 0.153
6.425IleLys: 6.425 ± 0.099
7.47IleLeu: 7.47 ± 0.133
2.277IleMet: 2.277 ± 0.06
4.911IleAsn: 4.911 ± 0.096
3.107IlePro: 3.107 ± 0.069
3.07IleGln: 3.07 ± 0.067
2.642IleArg: 2.642 ± 0.058
5.093IleSer: 5.093 ± 0.093
4.973IleThr: 4.973 ± 0.104
5.865IleVal: 5.865 ± 0.092
0.59IleTrp: 0.59 ± 0.032
3.148IleTyr: 3.148 ± 0.069
0.0IleXaa: 0.0 ± 0.0
Lys
4.846LysAla: 4.846 ± 0.091
0.371LysCys: 0.371 ± 0.02
5.049LysAsp: 5.049 ± 0.09
6.192LysGlu: 6.192 ± 0.13
2.74LysPhe: 2.74 ± 0.064
4.134LysGly: 4.134 ± 0.084
1.708LysHis: 1.708 ± 0.053
5.444LysIle: 5.444 ± 0.093
6.033LysLys: 6.033 ± 0.124
6.5LysLeu: 6.5 ± 0.102
2.5LysMet: 2.5 ± 0.058
3.975LysAsn: 3.975 ± 0.088
2.168LysPro: 2.168 ± 0.055
3.32LysGln: 3.32 ± 0.074
3.117LysArg: 3.117 ± 0.063
4.431LysSer: 4.431 ± 0.074
4.079LysThr: 4.079 ± 0.084
5.316LysVal: 5.316 ± 0.109
0.558LysTrp: 0.558 ± 0.029
3.128LysTyr: 3.128 ± 0.078
0.0LysXaa: 0.0 ± 0.0
Leu
5.911LeuAla: 5.911 ± 0.084
0.562LeuCys: 0.562 ± 0.029
5.038LeuAsp: 5.038 ± 0.103
5.925LeuGlu: 5.925 ± 0.105
4.634LeuPhe: 4.634 ± 0.12
5.657LeuGly: 5.657 ± 0.105
1.698LeuHis: 1.698 ± 0.053
8.031LeuIle: 8.031 ± 0.137
7.12LeuLys: 7.12 ± 0.113
9.055LeuLeu: 9.055 ± 0.156
2.867LeuMet: 2.867 ± 0.069
5.588LeuAsn: 5.588 ± 0.098
3.462LeuPro: 3.462 ± 0.069
2.803LeuGln: 2.803 ± 0.074
2.865LeuArg: 2.865 ± 0.074
6.554LeuSer: 6.554 ± 0.093
5.482LeuThr: 5.482 ± 0.091
5.419LeuVal: 5.419 ± 0.088
0.574LeuTrp: 0.574 ± 0.033
3.283LeuTyr: 3.283 ± 0.073
0.0LeuXaa: 0.0 ± 0.0
Met
1.773MetAla: 1.773 ± 0.053
0.152MetCys: 0.152 ± 0.017
1.633MetAsp: 1.633 ± 0.046
1.57MetGlu: 1.57 ± 0.051
1.239MetPhe: 1.239 ± 0.048
1.601MetGly: 1.601 ± 0.046
0.741MetHis: 0.741 ± 0.035
2.731MetIle: 2.731 ± 0.066
2.608MetLys: 2.608 ± 0.064
2.786MetLeu: 2.786 ± 0.072
1.073MetMet: 1.073 ± 0.045
1.903MetAsn: 1.903 ± 0.048
1.06MetPro: 1.06 ± 0.034
1.06MetGln: 1.06 ± 0.038
1.109MetArg: 1.109 ± 0.04
1.874MetSer: 1.874 ± 0.044
1.874MetThr: 1.874 ± 0.056
1.483MetVal: 1.483 ± 0.045
0.193MetTrp: 0.193 ± 0.018
1.042MetTyr: 1.042 ± 0.041
0.0MetXaa: 0.0 ± 0.0
Asn
3.3AsnAla: 3.3 ± 0.067
0.308AsnCys: 0.308 ± 0.024
3.282AsnAsp: 3.282 ± 0.063
4.098AsnGlu: 4.098 ± 0.087
2.13AsnPhe: 2.13 ± 0.056
3.193AsnGly: 3.193 ± 0.068
1.356AsnHis: 1.356 ± 0.05
4.909AsnIle: 4.909 ± 0.097
4.359AsnLys: 4.359 ± 0.091
4.267AsnLeu: 4.267 ± 0.079
1.41AsnMet: 1.41 ± 0.044
3.3AsnAsn: 3.3 ± 0.093
2.048AsnPro: 2.048 ± 0.05
2.342AsnGln: 2.342 ± 0.064
2.038AsnArg: 2.038 ± 0.051
2.254AsnSer: 2.254 ± 0.061
2.799AsnThr: 2.799 ± 0.097
3.515AsnVal: 3.515 ± 0.078
0.375AsnTrp: 0.375 ± 0.027
2.14AsnTyr: 2.14 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
1.818ProAla: 1.818 ± 0.054
0.178ProCys: 0.178 ± 0.018
1.794ProAsp: 1.794 ± 0.054
2.709ProGlu: 2.709 ± 0.066
1.826ProPhe: 1.826 ± 0.048
2.137ProGly: 2.137 ± 0.089
0.714ProHis: 0.714 ± 0.034
2.655ProIle: 2.655 ± 0.057
2.229ProLys: 2.229 ± 0.058
2.923ProLeu: 2.923 ± 0.074
0.903ProMet: 0.903 ± 0.045
1.65ProAsn: 1.65 ± 0.045
0.772ProPro: 0.772 ± 0.053
0.923ProGln: 0.923 ± 0.035
0.893ProArg: 0.893 ± 0.038
1.739ProSer: 1.739 ± 0.049
1.975ProThr: 1.975 ± 0.09
2.505ProVal: 2.505 ± 0.071
0.264ProTrp: 0.264 ± 0.02
1.275ProTyr: 1.275 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
2.123GlnAla: 2.123 ± 0.065
0.196GlnCys: 0.196 ± 0.017
1.846GlnAsp: 1.846 ± 0.056
2.145GlnGlu: 2.145 ± 0.06
1.839GlnPhe: 1.839 ± 0.046
1.838GlnGly: 1.838 ± 0.056
0.855GlnHis: 0.855 ± 0.035
2.84GlnIle: 2.84 ± 0.068
2.563GlnLys: 2.563 ± 0.058
3.866GlnLeu: 3.866 ± 0.09
1.225GlnMet: 1.225 ± 0.045
1.884GlnAsn: 1.884 ± 0.051
1.111GlnPro: 1.111 ± 0.046
1.537GlnGln: 1.537 ± 0.052
1.212GlnArg: 1.212 ± 0.045
2.346GlnSer: 2.346 ± 0.058
1.948GlnThr: 1.948 ± 0.061
2.134GlnVal: 2.134 ± 0.053
0.292GlnTrp: 0.292 ± 0.022
1.681GlnTyr: 1.681 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
2.144ArgAla: 2.144 ± 0.057
0.191ArgCys: 0.191 ± 0.017
2.04ArgAsp: 2.04 ± 0.055
2.481ArgGlu: 2.481 ± 0.071
1.695ArgPhe: 1.695 ± 0.05
2.064ArgGly: 2.064 ± 0.058
0.796ArgHis: 0.796 ± 0.038
3.077ArgIle: 3.077 ± 0.071
2.791ArgLys: 2.791 ± 0.068
3.419ArgLeu: 3.419 ± 0.071
1.143ArgMet: 1.143 ± 0.044
1.958ArgAsn: 1.958 ± 0.048
1.046ArgPro: 1.046 ± 0.043
1.286ArgGln: 1.286 ± 0.044
1.56ArgArg: 1.56 ± 0.051
1.763ArgSer: 1.763 ± 0.05
1.907ArgThr: 1.907 ± 0.053
2.329ArgVal: 2.329 ± 0.061
0.243ArgTrp: 0.243 ± 0.017
1.513ArgTyr: 1.513 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
3.266SerAla: 3.266 ± 0.127
0.35SerCys: 0.35 ± 0.025
3.289SerAsp: 3.289 ± 0.133
3.949SerGlu: 3.949 ± 0.078
2.92SerPhe: 2.92 ± 0.066
3.853SerGly: 3.853 ± 0.078
1.379SerHis: 1.379 ± 0.044
5.286SerIle: 5.286 ± 0.104
4.199SerLys: 4.199 ± 0.083
5.414SerLeu: 5.414 ± 0.102
1.578SerMet: 1.578 ± 0.047
2.999SerAsn: 2.999 ± 0.069
1.682SerPro: 1.682 ± 0.05
1.898SerGln: 1.898 ± 0.058
2.0SerArg: 2.0 ± 0.055
3.213SerSer: 3.213 ± 0.084
3.482SerThr: 3.482 ± 0.218
3.948SerVal: 3.948 ± 0.129
0.442SerTrp: 0.442 ± 0.027
2.301SerTyr: 2.301 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
3.599ThrAla: 3.599 ± 0.139
0.349ThrCys: 0.349 ± 0.024
3.179ThrAsp: 3.179 ± 0.097
3.853ThrGlu: 3.853 ± 0.1
2.769ThrPhe: 2.769 ± 0.068
3.897ThrGly: 3.897 ± 0.143
1.345ThrHis: 1.345 ± 0.05
4.939ThrIle: 4.939 ± 0.086
3.772ThrLys: 3.772 ± 0.078
5.746ThrLeu: 5.746 ± 0.127
1.435ThrMet: 1.435 ± 0.05
2.765ThrAsn: 2.765 ± 0.092
2.277ThrPro: 2.277 ± 0.083
1.754ThrGln: 1.754 ± 0.063
1.791ThrArg: 1.791 ± 0.052
3.52ThrSer: 3.52 ± 0.213
3.585ThrThr: 3.585 ± 0.181
4.083ThrVal: 4.083 ± 0.11
0.391ThrTrp: 0.391 ± 0.024
2.296ThrTyr: 2.296 ± 0.059
0.0ThrXaa: 0.0 ± 0.0
Val
4.233ValAla: 4.233 ± 0.091
0.545ValCys: 0.545 ± 0.03
3.927ValAsp: 3.927 ± 0.086
4.607ValGlu: 4.607 ± 0.093
2.803ValPhe: 2.803 ± 0.067
4.068ValGly: 4.068 ± 0.085
1.239ValHis: 1.239 ± 0.042
6.27ValIle: 6.27 ± 0.092
5.142ValLys: 5.142 ± 0.104
6.076ValLeu: 6.076 ± 0.099
1.982ValMet: 1.982 ± 0.066
3.522ValAsn: 3.522 ± 0.069
2.392ValPro: 2.392 ± 0.068
2.109ValGln: 2.109 ± 0.046
2.223ValArg: 2.223 ± 0.062
4.186ValSer: 4.186 ± 0.13
4.397ValThr: 4.397 ± 0.174
4.85ValVal: 4.85 ± 0.093
0.463ValTrp: 0.463 ± 0.029
2.36ValTyr: 2.36 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
0.411TrpAla: 0.411 ± 0.025
0.069TrpCys: 0.069 ± 0.009
0.366TrpAsp: 0.366 ± 0.026
0.356TrpGlu: 0.356 ± 0.021
0.378TrpPhe: 0.378 ± 0.025
0.443TrpGly: 0.443 ± 0.026
0.195TrpHis: 0.195 ± 0.018
0.711TrpIle: 0.711 ± 0.034
0.419TrpLys: 0.419 ± 0.024
0.826TrpLeu: 0.826 ± 0.035
0.288TrpMet: 0.288 ± 0.019
0.395TrpAsn: 0.395 ± 0.026
0.217TrpPro: 0.217 ± 0.019
0.296TrpGln: 0.296 ± 0.021
0.26TrpArg: 0.26 ± 0.017
0.432TrpSer: 0.432 ± 0.025
0.364TrpThr: 0.364 ± 0.021
0.411TrpVal: 0.411 ± 0.024
0.085TrpTrp: 0.085 ± 0.013
0.315TrpTyr: 0.315 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.436TyrAla: 2.436 ± 0.061
0.291TyrCys: 0.291 ± 0.02
2.527TyrAsp: 2.527 ± 0.061
2.827TyrGlu: 2.827 ± 0.07
2.175TyrPhe: 2.175 ± 0.059
2.384TyrGly: 2.384 ± 0.06
1.009TyrHis: 1.009 ± 0.038
3.276TyrIle: 3.276 ± 0.084
2.877TyrLys: 2.877 ± 0.06
3.763TyrLeu: 3.763 ± 0.085
0.925TyrMet: 0.925 ± 0.035
2.102TyrAsn: 2.102 ± 0.051
1.318TyrPro: 1.318 ± 0.047
1.698TyrGln: 1.698 ± 0.058
1.481TyrArg: 1.481 ± 0.043
2.195TyrSer: 2.195 ± 0.06
2.028TyrThr: 2.028 ± 0.058
2.322TyrVal: 2.322 ± 0.063
0.318TyrTrp: 0.318 ± 0.018
1.795TyrTyr: 1.795 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2430 proteins (708472 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski